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Gene information
General information
TC003133 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 223694..237724
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003133
    iBeetle screen iB_03503
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003133 RA
      MANTAHLVR RQSSRDLNP QRSVDRLEL REMRGRLVV 
      ENGTTATYG TANAAFEDS SPNTKIKSS CSSKRGSQD 
      GKAIFRPES GEDERESWD SKLTFLLAT VGYAVGLGN 
      VWRFPYLAQ KNGGGAFLI PYFVMLAVE GIPIFYLEL 
      AIGQRLRKG AIGVWNQVS PFLSGIGIS SAVVSFNVA 
      LYYNTIIAW CLFYFVQSF QSQLPWAEC PKVYFPNGS 
      YTSEPECVV SSPTQYFWY RTTLMISDD INSPEAFNW 
      KIALALVVA WILVYMCMI KGIASSGKV VYVTATFPY 
      IVLIIFFFR GITLKGAGD GLRHLFTPS WHKILEPVV 
      WLEAGTQIF FSLGLAFGG LIAFSSYNP VNNNCYRDA 
      LMVSLTNCF TSMFAGIVV FSIIGFKAT MVYDKCMST 
      RNSTLAALF GNNDFDESR LPPKGTVFN VSGEDGSIV 
      SVVMPLLPV CDLDKELDN SASGTGLAF IIFTEAINQ 
      FPGAQFWSV LFFLMLFTL GIDSQFGTL EGVVTSIVD 
      LKLFPNLPK EILTGGICL TCCLISMAF AHGAGSYIF 
      VLFDGYSGN FPLLIIALF ECIGVAYVY GLKRFADDI 
      EMMTGQRPA LYWLLCWKY LSPLAMMSI LVSSFIEIA 
      VDGSKYDAW VASKGETEK HDWPIWSVV LILVLVFSS 
      IAWIPGAAI ARLFGTVLI DDNEKAWFP ANDLKEFHG 
      IMPHEVSKA ETLLFCIHA DGTEGLCCP TKYTNDDDE 
      DLD
    CDS sequence
    • >TC003133 RA
      atggcaaac acggcccat ttagtacgt agacagagc 
      tcccgcgac ctaaacccc caacgttcg gtagaccgg 
      ctcgaattg cgggaaatg cgaggccgt ttagtggtg 
      gaaaacggc accacagcc acctacggc actgcaaac 
      gccgccttc gaggactcc agccccaac actaagatc 
      aagtccagc tgcagcagc aaacgcggc tcccaggac 
      ggaaaagcc attttccgg cccgagtcc ggcgaagac 
      gagcgcgag tcctgggac agcaagctg actttcctc 
      ctggcgacc gtgggctac gccgtgggg ctgggcaac 
      gtctggagg ttcccctac ttggcgcag aagaacggg 
      ggcggcgcc ttcctcatc ccttatttc gtgatgttg 
      gcggttgaa ggcatcccg attttttat ctggagctg 
      gctatcggc cagaggctg agaaagggg gccatcggt 
      gtctggaat caagtgtcg ccctttctg tccggaatt 
      gggataagc agtgcagtg gtgtcgttc aacgtggcg 
      ctttattac aacacgata attgcctgg tgtttgttt 
      tatttcgtg cagagtttc caatcgcaa ctgccgtgg 
      gctgagtgc cccaaggtg tatttccct aatggctcc 
      tacacttcc gaaccggaa tgtgtggtc agcagcccc 
      acgcagtac ttctggtac cgcacgacc ctaatgatc 
      tctgacgac atcaacagt ccggaagct ttcaactgg 
      aagattgct ctggctttg gtcgtggcc tggatacta 
      gtttacatg tgcatgatc aaaggaatt gcctcttca 
      gggaaagtc gtttacgtg acggcgacc ttcccttac 
      atcgtcctt ataatcttc ttttttcgc gggataacg 
      ctgaagggt gccggcgat ggcctaaga catctgttt 
      acgccctcc tggcacaag atactggaa ccggtggtt 
      tggctggag gctggcact caaattttc ttctccttg 
      ggacttgca tttggaggc cttatcgcg ttttcttcg 
      tataatcct gttaataat aattgttat agagacgcc 
      ttgatggtg tctctaacg aactgtttt acttcgatg 
      tttgctgga attgtggtt ttttccatc attgggttc 
      aaagctacg atggtttat gacaagtgc atgagcacg 
      aggaatagc actcttgct gcactgttt ggcaacaac 
      gattttgac gaaagtaga ctacctccg aaagggaca 
      gttttcaat gtttcgggc gaagatggc tcgattgtt 
      agcgttgtg atgccttta ctgccagta tgtgactta 
      gacaaagaa ctggacaat tcagcctct ggtacagga 
      ttagctttt ataatattt acagaagca ataaatcaa 
      tttcccggc gcccaattc tggtctgtt cttttcttc 
      ctgatgctg ttcacattg ggaattgat tcgcagttc 
      gggacgttg gagggagtc gtcacgtcc attgtggac 
      ttgaaacta tttccaaac ttgccgaaa gagatactg 
      actggtgga atttgttta acctgttgc cttatttca 
      atggcgttt gctcatggg gccggaagt tacattttt 
      gtgcttttt gatggctac agcgggaac tttccgcta 
      ctaatcata gctttgttc gaatgcatt ggggtagcg 
      tacgtatac gggttaaaa agatttgcc gacgacatt 
      gaaatgatg acaggacaa cgtccggcc ctttattgg 
      ctattatgt tggaagtac ttatcgccg ttggcgatg 
      atgtccatt ctcgtgtcc agttttatc gaaattgct 
      gttgacggc tccaagtat gatgcctgg gttgcctcc 
      aagggggag acggaaaaa cacgattgg cctatttgg 
      tcggttgtt cttattctg gttctagtt ttcagttcc 
      attgcctgg attcctggc gctgctatt gccagattg 
      tttggaaca gttctaatc gatgataat gaaaaggcg 
      tggtttcca gccaacgat ttgaaggag tttcacggt 
      attatgccg catgaagtg tccaaagct gaaactctg 
      ttattttgc atacacgct gacggaacc gaagggctt 
      tgctgtcca acgaagtac acaaatgac gatgacgaa 
      gaccttgac taa
    mRNA sequence
    • >TC003133 RA
      ccgacagga ggttgatgc cgtcctcaa catccacag 
      ctcaagatg ccaaaccat aaagacaat aatatttag 
      gattaagta aagcactcg aaccgagat ggcaaacac 
      ggcccattt agtacgtag acagagctc ccgcgacct 
      aaaccccca acgttcggt agaccggct cgaattgcg 
      ggaaatgcg aggccgttt agtggtgga aaacggcac 
      cacagccac ctacggcac tgcaaacgc cgccttcga 
      ggactccag ccccaacac taagatcaa gtccagctg 
      cagcagcaa acgcggctc ccaggacgg aaaagccat 
      tttccggcc cgagtccgg cgaagacga gcgcgagtc 
      ctgggacag caagctgac tttcctcct ggcgaccgt 
      gggctacgc cgtggggct gggcaacgt ctggaggtt 
      cccctactt ggcgcagaa gaacggggg cggcgcctt 
      cctcatccc ttatttcgt gatgttggc ggttgaagg 
      catcccgat tttttatct ggagctggc tatcggcca 
      gaggctgag aaagggggc catcggtgt ctggaatca 
      agtgtcgcc ctttctgtc cggaattgg gataagcag 
      tgcagtggt gtcgttcaa cgtggcgct ttattacaa 
      cacgataat tgcctggtg tttgtttta tttcgtgca 
      gagtttcca atcgcaact gccgtgggc tgagtgccc 
      caaggtgta tttccctaa tggctccta cacttccga 
      accggaatg tgtggtcag cagccccac gcagtactt 
      ctggtaccg cacgaccct aatgatctc tgacgacat 
      caacagtcc ggaagcttt caactggaa gattgctct 
      ggctttggt cgtggcctg gatactagt ttacatgtg 
      catgatcaa aggaattgc ctcttcagg gaaagtcgt 
      ttacgtgac ggcgacctt cccttacat cgtccttat 
      aatcttctt ttttcgcgg gataacgct gaagggtgc 
      cggcgatgg cctaagaca tctgtttac gccctcctg 
      gcacaagat actggaacc ggtggtttg gctggaggc 
      tggcactca aattttctt ctccttggg acttgcatt 
      tggaggcct tatcgcgtt ttcttcgta taatcctgt 
      taataataa ttgttatag agacgcctt gatggtgtc 
      tctaacgaa ctgttttac ttcgatgtt tgctggaat 
      tgtggtttt ttccatcat tgggttcaa agctacgat 
      ggtttatga caagtgcat gagcacgag gaatagcac 
      tcttgctgc actgtttgg caacaacga ttttgacga 
      aagtagact acctccgaa agggacagt tttcaatgt 
      ttcgggcga agatggctc gattgttag cgttgtgat 
      gcctttact gccagtatg tgacttaga caaagaact 
      ggacaattc agcctctgg tacaggatt agcttttat 
      aatatttac agaagcaat aaatcaatt tcccggcgc 
      ccaattctg gtctgttct tttcttcct gatgctgtt 
      cacattggg aattgattc gcagttcgg gacgttgga 
      gggagtcgt cacgtccat tgtggactt gaaactatt 
      tccaaactt gccgaaaga gatactgac tggtggaat 
      ttgtttaac ctgttgcct tatttcaat ggcgtttgc 
      tcatggggc cggaagtta catttttgt gctttttga 
      tggctacag cgggaactt tccgctact aatcatagc 
      tttgttcga atgcattgg ggtagcgta cgtatacgg 
      gttaaaaag atttgccga cgacattga aatgatgac 
      aggacaacg tccggccct ttattggct attatgttg 
      gaagtactt atcgccgtt ggcgatgat gtccattct 
      cgtgtccag ttttatcga aattgctgt tgacggctc 
      caagtatga tgcctgggt tgcctccaa gggggagac 
      ggaaaaaca cgattggcc tatttggtc ggttgttct 
      tattctggt tctagtttt cagttccat tgcctggat 
      tcctggcgc tgctattgc cagattgtt tggaacagt 
      tctaatcga tgataatga aaaggcgtg gtttccagc 
      caacgattt gaaggagtt tcacggtat tatgccgca 
      tgaagtgtc caaagctga aactctgtt attttgcat 
      acacgctga cggaaccga agggctttg ctgtccaac 
      gaagtacac aaatgacga tgacgaaga ccttgacta 
      aagttcaag acttagcat taaccaata tttatgaac 
      attccatat cacgtattc gtatactca tatcatttt 
      tccttagga ttgaaattg gtcttattt tttaaacga 
      attttgtca atttcagcc gaaaagaga caataaatt 
      ttaggacaa tagttgact gaaaaagac tagaaaatg 
      aacgtgttc aaaccaaat tttgatttg ttcgaacta 
      aaatgtgat atatttgca caaatgttg ttttgtcac 
      gcagcgaat gacagactc gtctttgca accattcgg 
      ctctttgat tttttatcc acaatcttg tgctaacga 
      catatcctt cagtaaatc agagcgaaa ctccacgcc 
      atttagagt ctgtcctaa gtctagtca atttgaaat 
      aattagaca ataatagtt ggtaagaat atcgatgtg 
      tagctcaat gtgattagt tgatttgtt gctctttgt 
      tgttaaccc ggcaggttt gtaaattct tggccggtt 
      ttaaagtaa tgtatgaca ataattaca cttatgatg 
      cataaatat ctgtgttac gagataaaa attatttta 
      tttattgct atgccgcta gtccttgtt gaatacgta 
      atcatgatg aattgtata aatgtataa aagtatgta 
      aatacaaat ctgttgaaa tgtggaaaa taaaatctg 
      aactgtaaa tcaagcaac aaactgtgt cttattttg 
      acagcaagt gtaaatttt tgtgacaat gcccagtat 
      caaggaaca gttggaaaa gcaaccccc tttaagaat 
      tcaaacaga tactgagat tccgattgt tacgctgtt 
      agcaggtag ttggggatt tatcacaat tgcaaatat 
      cacccccga gttttaggt accgtggga attacgtta 
      cttcttgtt tgttgctgt tgtggcggt gggggcggt 
      ggtcgaaac ccctaaaca ttaaaaatg tggctgtac 
      attatttag gtaataaag cgtccaaaa aaaa