Status
Please wait ...
Gene information
General information
TC003125 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1383108..1386535
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003125
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03501

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003125 RA
      MEPPQKGAS KPCLEGPII KISEDYGGL GAHFLDTLF 
      ENLNKINQI DTVTGITES NGSVRSRAI QIAHEIRHL 
      GVVENDIVV ICCRSHADQ TIVVLACLL IGAIVAPID 
      SELHHRECV GIVTQLKPK MCFCDLRTL KQIERILAE 
      TGITSKLVH FGDQQQYAI SFRKLLSNR QYPEAFKPI 
      TVEQPRKKA AFILATQGT TDTPRLVCL SHHFIYCQI 
      LVFLEAMGS PEKIISYYP LSWVMQVVL MCLCFEVPV 
      VRVLTGAFV ERTACKLIH DLKIDTAFL NTELALQLV 
      EHVAVKDFN LSCLKCVFI GSLATTSHD IKLLVTRLP 
      KVRFTQMYC TTETGAVIA CPPRDYSES LERCGTVGK 
      VVQNCKLRV VDIDSRNDV EVECSGELL VTSDCLMLG 
      YFKNMGATQ TVMEGGYFK TGDLARYDA SGWLYIEGR 
      ICEFIYIQG CKISPRKIE EIIMTHPFV KDTAVVSNS 
      KEVVACVIT KPDTKLDEN RLITFISER LPVQNWPTR 
      IVFMSDFPT TPLGKIRRD ELKEEVLTV KIEHSESQW 
      HVLG
    CDS sequence
    • >TC003125 RA
      atggagcct ccacaaaaa ggtgccagc aagccgtgc 
      cttgaagga ccgattatt aaaattagt gaggactat 
      ggcggactt ggggcccat tttttagac acattattc 
      gaaaactta aacaaaata aaccagata gacacagta 
      actggtata acggaaagt aacggctcc gttcgttca 
      agagccatt caaatagcg cacgaaatt cgccatttg 
      ggagtcgtc gaaaacgac atagtcgta atttgctgc 
      cgaagtcac gctgaccaa acgattgta gtcctcgcg 
      tgtcttctg atcggcgca atagtcgcc cccattgac 
      tcagaactg caccacagg gaatgtgtg ggaattgtg 
      actcaactc aagccaaaa atgtgcttt tgcgatctg 
      cgaacgttg aaacaaatc gaacgaatt ttggctgaa 
      actggaatc acgtccaaa cttgtgcac tttggcgac 
      cagcaacag tacgccata tccttccga aagttgctg 
      tcaaatcgt caatacccc gaagctttt aaacctata 
      accgttgaa caaccgaga aaaaaagcg gcttttatt 
      ttggcaact caagggaca acggacacg ccacgacta 
      gtctgcctc tcgcatcac ttcatctat tgccaaatt 
      ttggtgttt cttgaagcc atgggttcc cccgagaaa 
      atcatctcg tactaccct ttgtcctgg gtgatgcaa 
      gtcgttctg atgtgtctg tgtttcgaa gttccggtc 
      gtcagagtt ctgactggc gcttttgtc gaaagaacg 
      gcttgtaaa ctgatacat gatcttaaa attgacact 
      gctttcctg aacacggaa cttgcccta caactggtg 
      gagcacgta gctgtgaaa gattttaat ttatcatgt 
      ctcaagtgc gtttttatc ggaagtttg gccacaact 
      tcgcacgat attaaacta ctagttacc cgattaccg 
      aaagttagg tttactcaa atgtactgc actacggaa 
      acgggagct gttattgcg tgcccgcct agagactac 
      agcgagtct ttagaacgg tgtggaact gttggaaaa 
      gtggtgcag aattgtaaa ctcagagtg gttgatatt 
      gatagtcgg aatgatgtg gaagttgag tgttctgga 
      gaactgcta gttacaagc gattgtttg atgcttggg 
      tattttaaa aacatggga gcaacgcag acagtgatg 
      gaaggtggg tacttcaaa actggtgat cttgccaga 
      tacgatgct tccggctgg ctgtacatt gagggaaga 
      atctgcgag tttatatac atccaaggc tgtaaaata 
      tcgccacgg aaaattgag gaaataata atgacacac 
      ccgtttgtc aaagacaca gcagttgtc agcaacagt 
      aaagaagtg gtggcttgt gtgataacc aaacctgac 
      acaaaattg gacgaaaac cgattgatc acgtttatt 
      tcagagaga cttcctgtc cagaactgg ccaacacgt 
      atcgtgttt atgtcagac tttcctaca actccacta 
      ggaaaaatc cgacgggac gaattaaag gaggaggtc 
      ctaacggtt aaaatcgaa cacagcgaa agccagtgg 
      cacgttttg ggctaa
    mRNA sequence
    • >TC003125 RA
      aaagtcaag aaatgccaa aaatgtcat taaaagtga 
      cactttaat caaaaataa aaatgtaaa tgcgagtca 
      aaattttct gaataaaac tacactttt tgtaactgt 
      attatggag cctccacaa aaaggtgcc agcaagccg 
      tgccttgaa ggaccgatt attaaaatt agtgaggac 
      tatggcgga cttggggcc catttttta gacacatta 
      ttcgaaaac ttaaacaaa ataaaccag atagacaca 
      gtaactggt ataacggaa agtaacggc tccgttcgt 
      tcaagagcc attcaaata gcgcacgaa attcgccat 
      ttgggagtc gtcgaaaac gacatagtc gtaatttgc 
      tgccgaagt cacgctgac caaacgatt gtagtcctc 
      gcgtgtctt ctgatcggc gcaatagtc gcccccatt 
      gactcagaa ctgcaccac agggaatgt gtgggaatt 
      gtgactcaa ctcaagcca aaaatgtgc ttttgcgat 
      ctgcgaacg ttgaaacaa atcgaacga attttggct 
      gaaactgga atcacgtcc aaacttgtg cactttggc 
      gaccagcaa cagtacgcc atatccttc cgaaagttg 
      ctgtcaaat cgtcaatac cccgaagct tttaaacct 
      ataaccgtt gaacaaccg agaaaaaaa gcggctttt 
      attttggca actcaaggg acaacggac acgccacga 
      ctagtctgc ctctcgcat cacttcatc tattgccaa 
      attttggtg tttcttgaa gccatgggt tcccccgag 
      aaaatcatc tcgtactac cctttgtcc tgggtgatg 
      caagtcgtt ctgatgtgt ctgtgtttc gaagttccg 
      gtcgtcaga gttctgact ggcgctttt gtcgaaaga 
      acggcttgt aaactgata catgatctt aaaattgac 
      actgctttc ctgaacacg gaacttgcc ctacaactg 
      gtggagcac gtagctgtg aaagatttt aatttatca 
      tgtctcaag tgcgttttt atcggaagt ttggccaca 
      acttcgcac gatattaaa ctactagtt acccgatta 
      ccgaaagtt aggtttact caaatgtac tgcactacg 
      gaaacggga gctgttatt gcgtgcccg cctagagac 
      tacagcgag tctttagaa cggtgtgga actgttgga 
      aaagtggtg cagaattgt aaactcaga gtggttgat 
      attgatagt cggaatgat gtggaagtt gagtgttct 
      ggagaactg ctagttaca agcgattgt ttgatgctt 
      gggtatttt aaaaacatg ggagcaacg cagacagtg 
      atggaaggt gggtacttc aaaactggt gatcttgcc 
      agatacgat gcttccggc tggctgtac attgaggga 
      agaatctgc gagtttata tacatccaa ggctgtaaa 
      atatcgcca cggaaaatt gaggaaata ataatgaca 
      cacccgttt gtcaaagac acagcagtt gtcagcaac 
      agtaaagaa gtggtggct tgtgtgata accaaacct 
      gacacaaaa ttggacgaa aaccgattg atcacgttt 
      atttcagag agacttcct gtccagaac tggccaaca 
      cgtatcgtg tttatgtca gactttcct acaactcca 
      ctaggaaaa atccgacgg gacgaatta aaggaggag 
      gtcctaacg gttaaaatc gaacacagc gaaagccag 
      tggcacgtt ttgggctaa atacatatt gacacaaaa 
      ttttgtttt tttacgtga ttgaaaatc gataagttg 
      agttttatt agccgcatg atgcgttta agaaaacca 
      tcagtttgt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY