Status
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Gene information
General information
TC032331
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003095,TC003096
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1811780..1876600
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032331
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03498

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    iBeetle screen iB_03499

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032331 RA
      MQDQVDGME LETLYQRYC IRLKHALFL SCLTVAVVT 
      SAGLLVTTC VLQELEISL LPIVTMSVL TFSLFCILF 
      ASQFPFILE SETWALVSS LFITTIVTT SMLLLGQRH 
      APLPLFALL LAIHTMLPL SRAVALALA SIVTLAYMA 
      LSIVDRVND DKHPYYLQL IPEGVLLVA ASCTGLYYR 
      HMTEGAHRR TFVGTRTCI ESRVKLECE KEQQEQLLL 
      SVIPAYIAA EVKRSIMLK MADACNEHS NRSFHEMYV 
      QRHNNVSIL YADIVNFTP LSEQLSASD LVKTLNELF 
      GRFDQIAQD NQCMRIKIL GDCYYCVSG LPVSRPNHA 
      YNCVNMGLQ MIEAIRFVR EATGFNVDM RIGIHTGNV 
      LCGVLGLRK WQFDVWSDD VTLANHMES GGIAGRVHI 
      TRATLHQLG DRFEVEPGD GMNRESYLA DHKIETFLI 
      VPPKKPETE AQNGNARGP LATIAERHN SIGTEGGIY 
      GMATPSGPP TRSRPSSKM TKYVECWGA DKPFANIAE 
      STLAKNIEL TTLAMIESN LLPDRSTCL ECKKCWHSE 
      DLHPALLWF RNRKQESEY RSQPDPEFR CYIAFATSI 
      FLAMCIMQI ATIPRTVVL YGTLAPTLV VLTVFVYLC 
      WLDGCCGPR PPSDTPTDE PGACSPPGE VVTGSRCLR 
      LAIFLVSVT LISACAVVT LVDYNPEPL PATMRELQA 
      EKNGSVIDN RTLSTLINE FDAEIYSME PTYLYSSAL 
      ALAAISVFL RMGFILKLG LMVASVILH IFVYSYLDF 
      FRDYHNDKF FAIPFELKS ALVLAIVVI FFHILDRQI 
      EFTSRTDYL WKAKLKVEQ EEVETMRGI NKILLENIL 
      PAHVAEHFL ASRDTQELY HERYSCIAV MFASIPNYK 
      EFYDESDVN KQGLECLRL LNEIICEFD KLLLKPKFS 
      CIEKIKTIG STYMLASGL RPGAEENSK EASRKEEHI 
      IIALVDFAV SLMTSLDQI NRDAFQRFK LRVGLNHGP 
      VIAGVVGAQ KPQYDIWGN TVNVASRMD SCGIMGKIQ 
      VTEETAKIL MNAGYQCEC RGVTYVKGK GNLTTYFVK 
      TPFDDK
    CDS sequence
    • >TC032331 RA
      atgcaggac caagtggac gggatggaa ctggagaca 
      ctctaccaa agatattgt atccgcctc aaacacgct 
      ctcttcttg tcctgcttg acggtggcg gtcgtcacc 
      agcgcgggg ctgctggtc accacctgt gtcctccag 
      gagctggag atcagcctg ctgcccatc gtcaccatg 
      tcagtcctc accttctcc ctcttctgc atcttattc 
      gcctctcaa ttcccgttt atcctcgaa tcggaaacc 
      tgggctctg gtttcctcc ctctttatc accaccatc 
      gtcacaaca tccatgctc ctgctgggt caaagacac 
      gcaccgctc cctctcttc gccctcctg ctggccatc 
      cacaccatg ctgccctta tctagggca gtggccctc 
      gccctagcc tcgatcgtc accctggcc tacatggcc 
      ctgtcgatc gtcgacaga gtgaatgac gacaaacac 
      ccctattac ctccagctg attcccgag ggagtgctg 
      ctggtggcg gcgagctgc accggcctc tactaccgc 
      cacatgacc gagggcgcc cacaggagg acttttgtc 
      ggcactagg acctgcata gagtcccgg gtgaagctg 
      gagtgcgag aaggaacag caggagcag cttcttctg 
      agcgtcatt ccggcgtat attgcagca gaggtgaag 
      cgcagcatt atgttgaag atggcagac gcctgcaac 
      gagcactcg aaccggagt ttccacgag atgtacgtc 
      cagcgtcac aacaacgtc tcaatcctg tacgcagac 
      atcgtcaat ttcacgcca ttgtccgag caattatcg 
      gcttctgac ctcgtcaag accctcaac gagctgttc 
      ggccgtttc gaccaaata gcgcaggac aaccaatgc 
      atgaggatc aagatcctt ggagactgc tattattgt 
      gtgtcagga ttgccggtg tctcgaccc aaccacgcc 
      tacaattgt gtcaacatg ggcctgcaa atgatcgag 
      gccatccga tttgttcgc gaggcgact ggattcaac 
      gtggatatg aggatcggc atccacacc ggaaacgtg 
      ctttgtggt gttttggga ttacgaaaa tggcaattt 
      gacgtctgg agcgacgat gttacgctt gccaaccac 
      atggaatcc ggcggcata gcggggagg gttcacata 
      acacgagcc accttacac cagcttggg gatcggttt 
      gaagtggag ccgggagat ggtatgaac cgggagtct 
      tacctggct gatcataaa attgaaact ttcctcatt 
      gtgcccccg aagaaacct gaaactgaa gcgcagaac 
      gggaacgcc agaggccct ctggcgacg atcgctgaa 
      agacacaac agcatcggc acagagggc ggaatctac 
      gggatggca accccaagc ggaccgccg acgaggtcc 
      aggccgtcc agcaagatg accaagtac gtggagtgc 
      tggggggct gataagccc tttgccaac attgccgaa 
      tccacgctc gccaaaaac atcgagcta acgactttg 
      gccatgatc gagtctaat ctcttgccg gaccgtagc 
      acctgcctg gaatgcaag aaatgctgg cacagcgag 
      gatcttcat ccagccctc ctgtggttc cgcaaccga 
      aagcaagaa agcgaatac cgcagccag ccagacccg 
      gagttccgc tgctacata gctttcgcg acctccatt 
      ttcctagcg atgtgcatt atgcagatt gccaccata 
      cccaggact gtggttttg tacggcacg ctggcgcct 
      acgttggtg gttttgacc gtgtttgtg tatttgtgt 
      tggctggac ggctgttgt gggcctagg ccaccctca 
      gacaccccg accgatgaa ccgggggct tgttcaccg 
      cctggtgaa gtggtgacc ggaagcaga tgtctccga 
      cttgccatt ttcctggtg tctgtgacg ctgatttcg 
      gcctgcgct gttgttaca ctggtggat tataacccg 
      gagccgcta ccggcgact atgagagaa ctccaggcg 
      gaaaaaaat ggatccgtc atagacaac cgaacttta 
      agcactctg ataaacgaa tttgatgcg gagatttac 
      tcgatggaa ccgacctat ttgtacagc tcggcattg 
      gctttagct gcgatctct gtgttttta aggatgggc 
      ttcatcctg aagctggga ctcatggtc gcctctgtg 
      atattacat atcttcgtt tacagctat ttggacttc 
      tttagggac taccataat gataagttt ttcgcaata 
      ccgttcgaa ttaaaatcc gccctggtc ctcgcaatc 
      gtcgtgata tttttccac attctggac cgacaaatc 
      gagttcact tcacggacc gattatttg tggaaagcc 
      aagctcaag gtggagcaa gaagaagtg gaaacaatg 
      cgaggaatc aacaaaatt ctcttggaa aacatctta 
      cccgcccat gtggccgag cactttttg gcctccaga 
      gacacccaa gagctgtac cacgagcgg tactcatgt 
      atagccgta atgttcgct tcaataccc aactacaaa 
      gaattttac gatgaaagt gacgtcaat aaacaagga 
      ctggaatgt ctccggttg ctcaacgag atcatttgc 
      gagtttgac aaacttttg ctcaaacca aagtttagt 
      tgtatagag aagattaaa acaataggg agtacttac 
      atgttggcc tcgggcctc cgacctgga gccgaagaa 
      aacagcaag gaggcaagt cggaaggaa gagcacatt 
      attatcgct ctggtggat tttgctgtg tcgctgatg 
      acgagcctt gatcaaatt aaccgagac gcgttccaa 
      agattcaaa ctgagagtt ggtttgaat cacgggccg 
      gtgattgcg ggtgttgtg ggggcgcag aagccccag 
      tacgacatt tgggggaac acagtgaac gtcgcctcc 
      cgcatggac tcctgcggc atcatgggc aagatccag 
      gtgaccgaa gagacggcc aaaattttg atgaatgcg 
      ggataccag tgcgagtgc agaggcgtc acttacgtc 
      aaaggaaag ggcaactta acgacttac tttgtcaaa 
      acgcccttc gacgacaag tga
    mRNA sequence
    • >TC032331 RA
      cgtcgcgac gtgacgcgt ctttgcgat ttttcatga 
      ttcactctg gcagtgctt gagcgagaa aaatccacc 
      aaaatgcag gaccaagtg gacgggatg gaactggag 
      acactctac caaagatat tgtatccgc ctcaaacac 
      gctctcttc ttgtcctgc ttgacggtg gcggtcgtc 
      accagcgcg gggctgctg gtcaccacc tgtgtcctc 
      caggagctg gagatcagc ctgctgccc atcgtcacc 
      atgtcagtc ctcaccttc tccctcttc tgcatctta 
      ttcgcctct caattcccg tttatcctc gaatcggaa 
      acctgggct ctggtttcc tccctcttt atcaccacc 
      atcgtcaca acatccatg ctcctgctg ggtcaaaga 
      cacgcaccg ctccctctc ttcgccctc ctgctggcc 
      atccacacc atgctgccc ttatctagg gcagtggcc 
      ctcgcccta gcctcgatc gtcaccctg gcctacatg 
      gccctgtcg atcgtcgac agagtgaat gacgacaaa 
      cacccctat tacctccag ctgattccc gagggagtg 
      ctgctggtg gcggcgagc tgcaccggc ctctactac 
      cgccacatg accgagggc gcccacagg aggactttt 
      gtcggcact aggacctgc atagagtcc cgggtgaag 
      ctggagtgc gagaaggaa cagcaggag cagcttctt 
      ctgagcgtc attccggcg tatattgca gcagaggtg 
      aagcgcagc attatgttg aagatggca gacgcctgc 
      aacgagcac tcgaaccgg agtttccac gagatgtac 
      gtccagcgt cacaacaac gtctcaatc ctgtacgca 
      gacatcgtc aatttcacg ccattgtcc gagcaatta 
      tcggcttct gacctcgtc aagaccctc aacgagctg 
      ttcggccgt ttcgaccaa atagcgcag gacaaccaa 
      tgcatgagg atcaagatc cttggagac tgctattat 
      tgtgtgtca ggattgccg gtgtctcga cccaaccac 
      gcctacaat tgtgtcaac atgggcctg caaatgatc 
      gaggccatc cgatttgtt cgcgaggcg actggattc 
      aacgtggat atgaggatc ggcatccac accggaaac 
      gtgctttgt ggtgttttg ggattacga aaatggcaa 
      tttgacgtc tggagcgac gatgttacg cttgccaac 
      cacatggaa tccggcggc atagcgggg agggttcac 
      ataacacga gccacctta caccagctt ggggatcgg 
      tttgaagtg gagccggga gatggtatg aaccgggag 
      tcttacctg gctgatcat aaaattgaa actttcctc 
      attgtgccc ccgaagaaa cctgaaact gaagcgcag 
      aacgggaac gccagaggc cctctggcg acgatcgct 
      gaaagacac aacagcatc ggcacagag ggcggaatc 
      tacgggatg gcaacccca agcggaccg ccgacgagg 
      tccaggccg tccagcaag atgaccaag tacgtggag 
      tgctggggg gctgataag ccctttgcc aacattgcc 
      gaatccacg ctcgccaaa aacatcgag ctaacgact 
      ttggccatg atcgagtct aatctcttg ccggaccgt 
      agcacctgc ctggaatgc aagaaatgc tggcacagc 
      gaggatctt catccagcc ctcctgtgg ttccgcaac 
      cgaaagcaa gaaagcgaa taccgcagc cagccagac 
      ccggagttc cgctgctac atagctttc gcgacctcc 
      attttccta gcgatgtgc attatgcag attgccacc 
      atacccagg actgtggtt ttgtacggc acgctggcg 
      cctacgttg gtggttttg accgtgttt gtgtatttg 
      tgttggctg gacggctgt tgtgggcct aggccaccc 
      tcagacacc ccgaccgat gaaccgggg gcttgttca 
      ccgcctggt gaagtggtg accggaagc agatgtctc 
      cgacttgcc attttcctg gtgtctgtg acgctgatt 
      tcggcctgc gctgttgtt acactggtg gattataac 
      ccggagccg ctaccggcg actatgaga gaactccag 
      gcggaaaaa aatggatcc gtcatagac aaccgaact 
      ttaagcact ctgataaac gaatttgat gcggagatt 
      tactcgatg gaaccgacc tatttgtac agctcggca 
      ttggcttta gctgcgatc tctgtgttt ttaaggatg 
      ggcttcatc ctgaagctg ggactcatg gtcgcctct 
      gtgatatta catatcttc gtttacagc tatttggac 
      ttctttagg gactaccat aatgataag tttttcgca 
      ataccgttc gaattaaaa tccgccctg gtcctcgca 
      atcgtcgtg atatttttc cacattctg gaccgacaa 
      atcgagttc acttcacgg accgattat ttgtggaaa 
      gccaagctc aaggtggag caagaagaa gtggaaaca 
      atgcgagga atcaacaaa attctcttg gaaaacatc 
      ttacccgcc catgtggcc gagcacttt ttggcctcc 
      agagacacc caagagctg taccacgag cggtactca 
      tgtatagcc gtaatgttc gcttcaata cccaactac 
      aaagaattt tacgatgaa agtgacgtc aataaacaa 
      ggactggaa tgtctccgg ttgctcaac gagatcatt 
      tgcgagttt gacaaactt ttgctcaaa ccaaagttt 
      agttgtata gagaagatt aaaacaata gggagtact 
      tacatgttg gcctcgggc ctccgacct ggagccgaa 
      gaaaacagc aaggaggca agtcggaag gaagagcac 
      attattatc gctctggtg gattttgct gtgtcgctg 
      atgacgagc cttgatcaa attaaccga gacgcgttc 
      caaagattc aaactgaga gttggtttg aatcacggg 
      ccggtgatt gcgggtgtt gtgggggcg cagaagccc 
      cagtacgac atttggggg aacacagtg aacgtcgcc 
      tcccgcatg gactcctgc ggcatcatg ggcaagatc 
      caggtgacc gaagagacg gccaaaatt ttgatgaat 
      gcgggatac cagtgcgag tgcagaggc gtcacttac 
      gtcaaagga aagggcaac ttaacgact tactttgtc 
      aaaacgccc ttcgacgac aagtgaccc ggttgtgat 
      ccaaccacc aattccacg tactaatga gactgagga 
      tgttttggg tgattacgg gaactctca gctaatact 
      cacgatccc gtaatcacg agcaacggc actacattg 
      atagttaat ttattactt aatgtgttg tagtggagc 
      ggctgagcc gcttcggtt tcggaaatt tttatgtac 
      ctgactgaa tatttataa tgtaaatag taggtgttg 
      cttttaggt gaaagtttg aaacaccat taggtggtt 
      agactcgat ggctcctaa acttaatca ttttcggca 
      ttctaaatg tgattaggt aaatgtgtt agaccccgt 
      cgacaatcc tctccccgg tttattttt gtaacagta 
      tgacatttt taacgtata tttgtaatt ttgatgtga 
      agataaatg tgcgactag tttacgttt ggaataaat 
      cttgtttta tttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY