Status
Please wait ...
Gene information
General information
TC003092 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1892075..1917392
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003092
    iBeetle screen iB_03497

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition >5 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003092 RA
      MSSDKARGA PTELMPSIM GAFDAIIES NKSGNTPRK 
      SPQVGNFAN LSPCVQKIL ANVPDQELS KRFNSEETL 
      GPRRGNRTS IYRSLRSPE KHLNRSNES LDIISPNVQ 
      KMISNFPDA ELVLPSSER SKPSRNGSF LHSKSDGTP 
      KISQDFCVN SECDSLSVG EADSCDGFS SKPLGSYLH 
      TSHGIASRT PVGRKNMGK YLQVPSETS VGTSSTTSS 
      EVSRPVSLT SLGSCSSSG SSGPHQPSS AYLASAESL 
      DSDPEPTGS QGSADSGIA EQPTMSPEA RVLQEVLDT 
      ETVYVADLN EVIQGYLVP WKEDPDCLL CEHLPHLFS 
      NLEEIYRFN RSFLDQLRA ADFNPTKIA NVFIQNDSG 
      FAVYNEYCT NYPRTMEVL SELTRDEKM ASLFREKQM 
      ALSHALPLG SYLLKPVQR ILKYHLLLQ RLSKQCDTD 
      HKPAVDLAL ATMTAVASS INSMKRKHE HAVRVHEIQ 
      SQLYGWTGP DLTTLGELI AEGTFRVNG ARGRRHVFL 
      FDKVLLMAK NKQDGALAY KSHIECSNL MLVEQVRGE 
      PLSFQVLPF DNPRLQCTL RARSPQNKR EWTLQIKRV 
      ILENYSAVI PNHARQLVM QLGQDVQET EDTTEKWSP 
      LNKNSTPHY LERRSRVRK SRDLSNRRA SSQDRAFPS 
      LGSWRRKSE PSMGMIPQY TAKTIPKKI SKLKKAKES 
      GAKFYTDLS DSENYDVIG ESNESLAVS PSVDSPDRC 
      ENGGEEPPK SNNLEKIVT DLLMQNEEF QKALNRQRR 
      TRASEPEPP TWFEDEARL KLPSKADSL PRSFQLNDQ 
      IDDDKNKNQ YHVLDESLK ENPDPDEQH ENDLASQLD 
      DTDYPEHKI YRKTAIRFS LLQRIRTLM SEEEKRTTK 
      YPLHKQGSK SMGEKIAHP DYADPQKLF TSCSSSRTN 
      LNVELSECE MMKEPERLD MTISEKEVL NEFEKRLND 
      STKLAPDTG QDDELNGSS QSELLEQEN VNSSQNSDS 
      YYESILEKS LVEEYTKDE NGRLVAKQD SFKGNENCF 
      YVQKFRSEK EKTLREVNG NGKSAIKRP SKAPPPIPV 
      KPSRLVIST KNISNVIMS TETIKSGTT NIQKLYIEQ 
      SKTKADLSH ETRNVNVSE KSWVKAMVG RFE
    CDS sequence
    • >TC003092 RA
      atgagctcg gacaaggcc cgaggagcg cctaccgag 
      ctgatgccg agtattatg ggcgccttt gacgcgatc 
      atagaaagc aacaaatct ggaaacacg ccccgaaaa 
      tcgccacaa gttggaaac ttcgccaat ctctccccc 
      tgcgtccag aagattcta gccaacgtc cccgaccaa 
      gagctcagc aagcgcttc aactcggag gaaaccctg 
      ggaccaaga cgcggcaac aggacgtcg atttacagg 
      tcgctgcgg tcgccggaa aagcacctg aaccggagc 
      aacgaatcg ctggatatc atttcacca aacgtgcaa 
      aaaatgatt tcgaatttt cccgacgct gagcttgtg 
      ctgccaagc agtgagagg agtaagcct agtcgaaac 
      ggctcgttt ttgcactcc aagagtgac gggacgccg 
      aaaatttct caagacttt tgtgtgaat agtgaatgt 
      gatagtttg agtgttggg gaagcggac tcctgtgac 
      ggttttagt tccaagccg ctcgggtcc tacctgcac 
      acctcccac gggatcgcc tccaggacc ccagtcggg 
      aggaagaac atggggaag tacctccag gttccgagt 
      gagacaagt gtgggcaca agcagtacg acgtcgtcg 
      gaggtttcc cgccctgtt tcgctcaca agcctgggg 
      tcgtgttcg tctagcggc agcagtggc ccccatcag 
      cccagttcg gcatacctt gcctctgcc gaaagcctg 
      gacagcgac cccgagccc accggaagc caaggttcg 
      gcagacagt ggcattgca gagcagcca acaatgagt 
      ccagaggcc cgagttctg caggaagtg ttagataca 
      gaaaccgta tacgtcgcc gacctcaac gaggtcatc 
      cagggctat ttggtgccg tggaaggaa gaccccgac 
      tgtttgctg tgcgagcat ttgcctcac ctattcagc 
      aacttggag gagatttat agattcaac aggtcgttc 
      cttgatcag ctccgtgcc gctgatttc aatccgaca 
      aaaatcgcc aacgtcttc attcagaac gactcaggt 
      ttcgccgtt tacaacgaa tactgcacc aactatcct 
      cgcacaatg gaagtacta agcgaattg acgcgtgat 
      gaaaagatg gcctcttta ttccgcgag aagcaaatg 
      gctctaagt cacgctttg cctttaggc tcgtattta 
      ctcaaacca gtccaaaga atacttaaa taccacctt 
      ttgctccag aggctttcg aagcaatgt gatacggat 
      cataagccg gcagtggac ttggctttg gccacaatg 
      actgcagtc gcgtcaagc attaactcg atgaaaagg 
      aagcacgaa catgcagtt agagtgcat gaaatacag 
      tcgcagctt tatgggtgg acggggccg gacctcacc 
      acccttggc gaactgatc gcagaggga acgttcagg 
      gttaatggg gctaggggc agaaggcat gtctttctc 
      tttgataag gttttactc atggccaag aacaagcaa 
      gatggggct ctagcctac aagtcgcac attgagtgc 
      tcaaatttg atgcttgtg gagcaagta cggggcgaa 
      cccttatca ttccaagtc ctgcctttc gataacccc 
      cgattgcag tgcactctt cgggcgcgg tcgccccaa 
      aacaagcga gagtggacg ctccaaatc aaaagagtc 
      attttagaa aattacagt gctgttata ccaaaccat 
      gcccgtcaa ctggtcatg caactgggc caagatgtt 
      caagaaaca gaagataca acagaaaag tggtcacct 
      ttgaacaaa aactcgacc ccgcattac ctcgaacgc 
      cgatcaaga gtgcgaaaa tctcgggac ttgtccaac 
      cgtcgcgct tcgtcccaa gaccgcgct ttcccatcg 
      ctgggcagc tggcggcgg aagtccgag cccagcatg 
      ggcatgatc ccgcagtac accgccaag accatcccc 
      aagaagatt tccaaactc aagaaagcc aaagagagc 
      ggtgctaaa ttctacacg gacttgtcc gattcggaa 
      aactacgac gtaatcggg gagtccaac gagagtttg 
      gcggtctcg ccgagtgtt gacagtcca gaccggtgc 
      gagaatggc ggtgaggag ccgccgaag agcaacaat 
      ctggagaag atcgtcacc gatttgctg atgcagaat 
      gaagagttc caaaaagcg ctgaacagg caaaggagg 
      acgcgggcc agtgaaccc gaaccaccg acgtggttt 
      gaggacgag gctagactg aagttaccc agtaaggcg 
      gattctttg cccagatct ttccaactc aatgatcaa 
      atcgacgac gataaaaac aaaaaccaa tatcacgtt 
      ttggatgag tcattgaaa gagaatccc gaccctgat 
      gagcaacac gaaaatgat ctcgcgtct caactcgat 
      gacaccgac tatccggaa cacaaaata taccgcaag 
      actgccatt cgcttcagt ttactgcaa cgaattcgg 
      acgctcatg tccgaggag gaaaagcgc accaccaag 
      tacccctta cacaagcaa ggctcaaaa agcatgggc 
      gagaaaatc gcccatccc gattacgcc gacccgcag 
      aaacttttc acctcgtgt agctccagc cggacgaac 
      ctaaacgtc gaactatcg gaatgcgaa atgatgaag 
      gagccggaa cgcttagat atgacaata agtgaaaaa 
      gaagtactt aacgaattc gagaagcgc cttaatgat 
      agtacaaaa cttgcacca gacacgggc caagatgac 
      gagttaaat ggttctagt cagagcgag ttgctagaa 
      caggaaaat gtcaattct agtcaaaat tcggatagt 
      tattatgag agcatactt gagaagagt ctagttgag 
      gagtatacg aaggacgag aacgggcga ctggtcgcc 
      aaacaggac agttttaaa ggtaacgag aactgtttc 
      tacgtccag aagttccga tcggagaag gagaaaacg 
      ctgagggaa gtgaatggc aatggcaaa agtgcaatc 
      aaaaggccc agcaaagct ccaccgccc attcccgtc 
      aaaccgagc agattggtt atcagtaca aagaacatt 
      tcaaacgta atcatgtca acggagaca ataaagtcg 
      ggtacaacc aacatccag aaactttac atagagcaa 
      agcaaaact aaagctgac ttaagtcac gagacgaga 
      aatgtgaac gtcagtgag aagagttgg gtgaaggca 
      atggttgga cgattcgaa tga
    mRNA sequence
    • >TC003092 RA
      tgggaagat taatggtcg atcgacagg tggatacaa 
      tgtacacca aaatgagct cggacaagg cccgaggag 
      cgcctaccg agctgatgc cgagtatta tgggcgcct 
      ttgacgcga tcatagaaa gcaacaaat ctggaaaca 
      cgccccgaa aatcgccac aagttggaa acttcgcca 
      atctctccc cctgcgtcc agaagattc tagccaacg 
      tccccgacc aagagctca gcaagcgct tcaactcgg 
      aggaaaccc tgggaccaa gacgcggca acaggacgt 
      cgatttaca ggtcgctgc ggtcgccgg aaaagcacc 
      tgaaccgga gcaacgaat cgctggata tcatttcac 
      caaacgtgc aaaaaatga tttcgaatt ttcccgacg 
      ctgagcttg tgctgccaa gcagtgaga ggagtaagc 
      ctagtcgaa acggctcgt ttttgcact ccaagagtg 
      acgggacgc cgaaaattt ctcaagact tttgtgtga 
      atagtgaat gtgatagtt tgagtgttg gggaagcgg 
      actcctgtg acggtttta gttccaagc cgctcgggt 
      cctacctgc acacctccc acgggatcg cctccagga 
      ccccagtcg ggaggaaga acatgggga agtacctcc 
      aggttccga gtgagacaa gtgtgggca caagcagta 
      cgacgtcgt cggaggttt cccgccctg tttcgctca 
      caagcctgg ggtcgtgtt cgtctagcg gcagcagtg 
      gcccccatc agcccagtt cggcatacc ttgcctctg 
      ccgaaagcc tggacagcg accccgagc ccaccggaa 
      gccaaggtt cggcagaca gtggcattg cagagcagc 
      caacaatga gtccagagg cccgagttc tgcaggaag 
      tgttagata cagaaaccg tatacgtcg ccgacctca 
      acgaggtca tccagggct atttggtgc cgtggaagg 
      aagaccccg actgtttgc tgtgcgagc atttgcctc 
      acctattca gcaacttgg aggagattt atagattca 
      acaggtcgt tccttgatc agctccgtg ccgctgatt 
      tcaatccga caaaaatcg ccaacgtct tcattcaga 
      acgactcag gtttcgccg tttacaacg aatactgca 
      ccaactatc ctcgcacaa tggaagtac taagcgaat 
      tgacgcgtg atgaaaaga tggcctctt tattccgcg 
      agaagcaaa tggctctaa gtcacgctt tgcctttag 
      gctcgtatt tactcaaac cagtccaaa gaatactta 
      aataccacc ttttgctcc agaggcttt cgaagcaat 
      gtgatacgg atcataagc cggcagtgg acttggctt 
      tggccacaa tgactgcag tcgcgtcaa gcattaact 
      cgatgaaaa ggaagcacg aacatgcag ttagagtgc 
      atgaaatac agtcgcagc tttatgggt ggacggggc 
      cggacctca ccacccttg gcgaactga tcgcagagg 
      gaacgttca gggttaatg gggctaggg gcagaaggc 
      atgtctttc tctttgata aggttttac tcatggcca 
      agaacaagc aagatgggg ctctagcct acaagtcgc 
      acattgagt gctcaaatt tgatgcttg tggagcaag 
      tacggggcg aacccttat cattccaag tcctgcctt 
      tcgataacc cccgattgc agtgcactc ttcgggcgc 
      ggtcgcccc aaaacaagc gagagtgga cgctccaaa 
      tcaaaagag tcattttag aaaattaca gtgctgtta 
      taccaaacc atgcccgtc aactggtca tgcaactgg 
      gccaagatg ttcaagaaa cagaagata caacagaaa 
      agtggtcac ctttgaaca aaaactcga ccccgcatt 
      acctcgaac gccgatcaa gagtgcgaa aatctcggg 
      acttgtcca accgtcgcg cttcgtccc aagaccgcg 
      ctttcccat cgctgggca gctggcggc ggaagtccg 
      agcccagca tgggcatga tcccgcagt acaccgcca 
      agaccatcc ccaagaaga tttccaaac tcaagaaag 
      ccaaagaga gcggtgcta aattctaca cggacttgt 
      ccgattcgg aaaactacg acgtaatcg gggagtcca 
      acgagagtt tggcggtct cgccgagtg ttgacagtc 
      cagaccggt gcgagaatg gcggtgagg agccgccga 
      agagcaaca atctggaga agatcgtca ccgatttgc 
      tgatgcaga atgaagagt tccaaaaag cgctgaaca 
      ggcaaagga ggacgcggg ccagtgaac ccgaaccac 
      cgacgtggt ttgaggacg aggctagac tgaagttac 
      ccagtaagg cggattctt tgcccagat ctttccaac 
      tcaatgatc aaatcgacg acgataaaa acaaaaacc 
      aatatcacg ttttggatg agtcattga aagagaatc 
      ccgaccctg atgagcaac acgaaaatg atctcgcgt 
      ctcaactcg atgacaccg actatccgg aacacaaaa 
      tataccgca agactgcca ttcgcttca gtttactgc 
      aacgaattc ggacgctca tgtccgagg aggaaaagc 
      gcaccacca agtacccct tacacaagc aaggctcaa 
      aaagcatgg gcgagaaaa tcgcccatc ccgattacg 
      ccgacccgc agaaacttt tcacctcgt gtagctcca 
      gccggacga acctaaacg tcgaactat cggaatgcg 
      aaatgatga aggagccgg aacgcttag atatgacaa 
      taagtgaaa aagaagtac ttaacgaat tcgagaagc 
      gccttaatg atagtacaa aacttgcac cagacacgg 
      gccaagatg acgagttaa atggttcta gtcagagcg 
      agttgctag aacaggaaa atgtcaatt ctagtcaaa 
      attcggata gttattatg agagcatac ttgagaaga 
      gtctagttg aggagtata cgaaggacg agaacgggc 
      gactggtcg ccaaacagg acagtttta aaggtaacg 
      agaactgtt tctacgtcc agaagttcc gatcggaga 
      aggagaaaa cgctgaggg aagtgaatg gcaatggca 
      aaagtgcaa tcaaaaggc ccagcaaag ctccaccgc 
      ccattcccg tcaaaccga gcagattgg ttatcagta 
      caaagaaca tttcaaacg taatcatgt caacggaga 
      caataaagt cgggtacaa ccaacatcc agaaacttt 
      acatagagc aaagcaaaa ctaaagctg acttaagtc 
      acgagacga gaaatgtga acgtcagtg agaagagtt 
      gggtgaagg caatggttg gacgattcg aatgacaca 
      attcataat caaactttc ccatgtgct atatgtggc 
      tgttctagt tttatcctt gttcgtttg aagagtgtt 
      tatgatagt tgataaaat atttatttt taaatacaa 
      agcaaaaca aaggaaatt attgtacag ttttaacgc 
      tacggttaa aaatgtaag cattttaaa ctacgtatt 
      taccatagt aaattaaac gtgccaaat gggtcctta 
      ttttcagga ccttttaaa ttatttgtt aaaggtagc 
      actaatttt gtaaaagta tca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY