Status
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Gene information
General information
TC003091 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1963642..1968299
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003091
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03496

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants without visible segmentation but polarity - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs number increased - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment potentially transformed - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003091 RA
      MVKCKECGC NCKRCLAHD HDVHLHVEI ENLKQKLVE 
      RENHIVTME TNFLNEANK YPSGELVAL REELLTWQD 
      KYRRLYDAH RRVQRVNQG LEDKLLKLV DVCETEKST 
      LTKHVATLS QKLAEANHT IKKLTEDNE RYKNDVSLA 
      IQFLQCKQS NFVTRKFDS LPHEVQVQV SSHMTNKRK 
      PEEKKVSYE MKSIKVPIP TFPPTAMVY SVPKSPKPD 
      RKLELESES PPVDLVSAA IMAKVLEER QKEKAYAKH 
      CDTCTCSKK LSLVCDIAQ YTVGTQTGE YKSSLCLRC 
      NENLPQSPQ KMRIVKHDP SNKKVPIYP NESTEISGN 
      NIVKVQPNN FLIDETPNS LLSQPDINF FSINEAPRV 
      RNKSPKLGI KVEHHHLCN KTSPTYEPS DTSAKNGSS 
      DNLKGPRYC SMRLQSGSR NILLDNAHN NVAPVLYKQ 
      HKVKREETP TIHETSHTE SCSSEDLKN MSVTSDHSG 
      HNQQVADWI RNNLENDIS SSENSQSEP LIHGKRGNL 
      RGVDQIKYA EMEENVKRF LFGESEFLK TVEIGKLKY 
      QSYREKDGG NGNESNSSR SNSHTETEI
    CDS sequence
    • >TC003091 RA
      atggtgaag tgtaaggaa tgtggctgt aattgtaaa 
      agatgtttg gctcacgac catgacgta catttacac 
      gtcgaaata gaaaatctc aaacaaaag ttagtggag 
      cgagaaaat cacattgtt acaatggag actaatttt 
      ttaaacgag gctaataag taccccagt ggcgagttg 
      gtggccctt agggaggag ctactgaca tggcaggat 
      aagtacagg aggttgtat gatgctcac agacgagtc 
      caaagagta aaccaaggt ctagaagat aagctgttg 
      aaattagta gatgtgtgt gagactgag aaaagtacg 
      ttaactaaa catgtagct actttatcc cagaaacta 
      gcagaggct aatcatacc attaagaaa ttaacagaa 
      gataatgaa agatataaa aatgatgtt tctttagca 
      atacaattt cttcaatgc aaacagagt aattttgta 
      acgcgtaaa tttgattca ttaccccat gaagtacaa 
      gtgcaagtg tcttcacat atgacaaat aagcgaaaa 
      cccgaagaa aaaaaagtt tcttatgaa atgaaaagt 
      ataaaagtg ccgatcccg acatttccg ccaacagca 
      atggtctat tcagttcct aagtctcca aaacctgat 
      agaaaacta gaactagag tcagagtca cctcctgta 
      gatctagta tccgctgca ataatggca aaagtcctt 
      gaagagcgc caaaaggag aaagcttac gcgaaacat 
      tgtgatacc tgtacttgt tcaaaaaaa ctgtctcta 
      gtttgcgac atagcccag tacactgtt ggcacccaa 
      accggcgaa tacaaaagc tcactgtgt cttagatgt 
      aacgagaat cttccacaa agcccccaa aaaatgcgc 
      attgtaaag cacgacccg tcgaacaaa aaggtacca 
      atttacccc aacgaatcc accgaaatt tccggaaac 
      aacatcgtc aaagtgcaa ccaaataac ttcctgatt 
      gacgaaacg cccaacagt ttgctgtcc caacctgat 
      atcaatttt tttagtata aacgaagct cctcgtgtt 
      agaaacaag tccccgaag ctggggatc aaagtcgag 
      caccaccac ctgtgcaac aagacatcg cccacttac 
      gaaccctcg gacacaagt gccaaaaac gggtcttcc 
      gacaacctc aaaggcccc agatactgt tcaatgcgt 
      ttacagagc ggttctagg aacatactt ttagacaac 
      gctcacaat aacgtagct ccagttttg tataaacag 
      cacaaagtt aaacgagag gaaactcca accattcat 
      gaaacgtcg cacacggag tcttgctca agcgaagat 
      ttgaaaaac atgtccgta actagcgac cattcgggt 
      cataatcaa caagtcgca gactggata cgaaacaat 
      ctggaaaat gatataagt agttcggaa aattcgcag 
      tctgagccg ctcattcat ggaaaacgg ggcaatttg 
      agaggagtt gatcaaatc aagtacgcc gagatggag 
      gaaaatgtt aagcggttt ttatttgga gagagcgag 
      tttttgaaa actgtggaa atcgggaag ttgaagtat 
      cagagttac agggagaag gatgggggg aatggaaat 
      gaaagtaat agttctagg agtaattcg catacggaa 
      accgagatt taa
    mRNA sequence
    • >TC003091 RA
      agacacgta ttgtttaaa tttattgtt actatttca 
      ttgtgttac gtgcttaac aacatctaa gtgctcctc 
      cacatatta tcaagtgac caaaaaatt tgcctcaag 
      taataacct taaacactt tcgaagtga aagttttat 
      aagtttttt gttgtttaa tttgatatc cgggaagat 
      ttctcatct cacaattac gtggacgta acattattt 
      atttagtct tcaatatgg tgaagtgta aggaatgtg 
      gctgtaatt gtaaaagat gtttggctc acgaccatg 
      acgtacatt tacacgtcg aaatagaaa atctcaaac 
      aaaagttag tggagcgag aaaatcaca ttgttacaa 
      tggagacta attttttaa acgaggcta ataagtacc 
      ccagtggcg agttggtgg cccttaggg aggagctac 
      tgacatggc aggataagt acaggaggt tgtatgatg 
      ctcacagac gagtccaaa gagtaaacc aaggtctag 
      aagataagc tgttgaaat tagtagatg tgtgtgaga 
      ctgagaaaa gtacgttaa ctaaacatg tagctactt 
      tatcccaga aactagcag aggctaatc ataccatta 
      agaaattaa cagaagata atgaaagat ataaaaatg 
      atgtttctt tagcaatac aatttcttc aatgcaaac 
      agagtaatt ttgtaacgc gtaaatttg attcattac 
      cccatgaag tacaagtgc aagtgtctt cacatatga 
      caaataagc gaaaacccg aagaaaaaa aagtttctt 
      atgaaatga aaagtataa aagtgccga tcccgacat 
      ttccgccaa cagcaatgg tctattcag ttcctaagt 
      ctccaaaac ctgatagaa aactagaac tagagtcag 
      agtcacctc ctgtagatc tagtatccg ctgcaataa 
      tggcaaaag tccttgaag agcgccaaa aggagaaag 
      cttacgcga aacattgtg atacctgta cttgttcaa 
      aaaaactgt ctctagttt gcgacatag cccagtaca 
      ctgttggca cccaaaccg gcgaataca aaagctcac 
      tgtgtctta gatgtaacg agaatcttc cacaaagcc 
      cccaaaaaa tgcgcattg taaagcacg acccgtcga 
      acaaaaagg taccaattt accccaacg aatccaccg 
      aaatttccg gaaacaaca tcgtcaaag tgcaaccaa 
      ataacttcc tgattgacg aaacgccca acagtttgc 
      tgtcccaac ctgatatca attttttta gtataaacg 
      aagctcctc gtgttagaa acaagtccc cgaagctgg 
      ggatcaaag tcgagcacc accacctgt gcaacaaga 
      catcgccca cttacgaac cctcggaca caagtgcca 
      aaaacgggt cttccgaca acctcaaag gccccagat 
      actgttcaa tgcgtttac agagcggtt ctaggaaca 
      tacttttag acaacgctc acaataacg tagctccag 
      ttttgtata aacagcaca aagttaaac gagaggaaa 
      ctccaacca ttcatgaaa cgtcgcaca cggagtctt 
      gctcaagcg aagatttga aaaacatgt ccgtaacta 
      gcgaccatt cgggtcata atcaacaag tcgcagact 
      ggatacgaa acaatctgg aaaatgata taagtagtt 
      cggaaaatt cgcagtctg agccgctca ttcatggaa 
      aacggggca atttgagag gagttgatc aaatcaagt 
      acgccgaga tggaggaaa atgttaagc ggtttttat 
      ttggagaga gcgagtttt tgaaaactg tggaaatcg 
      ggaagttga agtatcaga gttacaggg agaaggatg 
      gggggaatg gaaatgaaa gtaatagtt ctaggagta 
      attcgcata cggaaaccg agatttaat aattttatg 
      gttttttaa tgtatgttt tttacacaa agtgcaatc 
      aatagtaga atttatttt tatgtagtg gtaggatcc 
      agagtgtga ggtgagtca agggcacaa agtgtgctt 
      tcactcttg aaactttgc aaatattaa aatactgac 
      cactaaaat tttcacgac ttggagaaa aaaaattta 
      aattggcgt ttagttaga tagactttt ttcaaagac 
      tgtttaact ttctataag aattttttg tcttgtatc 
      ttcaatatt ttctcaccg ttttgggaa atatgaggc 
      tgtgcgaaa attgatttg atttaattt ctttgtgag 
      tgaagagtt tgtgttaga actgaatcc tactgaaaa 
      aatttggcc caaaaaatt attttctcg aaaaaaaac 
      tggcgatat ttattgaac tatctgcct ttaaaaccc 
      agatgagtt aagaacact agatattct agtttctgc 
      ttcggctac aattttttt tttcgcaga atatctgga 
      aaaacatca gccagtggc tagctatta tcgtttatg 
      gttaaaatg agacggtat ctaattgcc tttgaaacc 
      ctaaatttt gttctttga tcaatgaaa gcgtcttgg 
      ctttttttt tggttctgt ttgtaagaa tttcacacc 
      taatatcat aaagtgaga acgttcatt tatctatta 
      ctattaatc atttcatcg ggttttcaa taactgata 
      taaaaaact acttcttta tttcttcaa aatattttt 
      ttatcgcat tttttcgga ttttattcg gtttaattt 
      attttggct tttagtctg tttgctatc cttaggtga 
      acgataact tctcaatta tgtcgtgct ataaaaata 
      ataatattt aataataat tcttacacg ttttacaca 
      agttattat agtctcata tttaaaaga aattaaaat 
      tacatttaa gaaactcat tcaagctct acaagcagt 
      aatattact tttaatctt gttttttaa taacttgcg 
      ttgcaattt aataatgta ggagtatta gttattatt 
      ttttctcaa agtgagtag tttaggtaa agaacgctt 
      ggtttcaca ctttttaca atcacgtca atgataata 
      aaattttct tgacagtcg cgctaaaat aaagggggg 
      aatacggaa ttatacttg ataaaaata gtgatcgtg 
      tattgtatt ttaaaattt ttaactttg agggacttg 
      tttatgtaa aaaataaaa atgtagcta attttgacg 
      agttctatt tgtggctaa ggacacaac ttttagaaa 
      attgaagct cgatataca aaattttta tttcactaa 
      ttttttaaa cttttctat ggtattgaa tcatttaat 
      acgctgtag atttttgcg cttgactca tttcatact 
      ttggaactt gctgtaatt atgcgaatg tgattcaaa 
      aaggtctaa atgaaatat tgtggcgaa aactagggg 
      aacatctgt aatatcaca agtagcatt aaatagtaa 
      aactagcat tgaagaatg gaacaaatt aagatgaaa 
      gcaatgcca gtatcagaa ttatttgca aatagacaa 
      aaaattagt acaaatagt tattagttc tactaaaat 
      tattagcac gtctttaaa caaaggttc ctggcgata 
      tgtgtcgga gtgtttaca gcagaaccc aatttatcc 
      gtgtaattc ggaaacaca catatcatg caaagtgtg 
      atggaaaca aaaaggcaa aattatgga taaacgggg 
      ttctattta acgtgcaat ttttcatac ctggtttgt 
      gtaaannnn nnnnnnnnn nnnnnnnnn
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY