Status
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Gene information
General information
TC032339
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003075,TC003076
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 2640727..2676086
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032339
    iBeetle screen iB_03493

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not completely molted - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection



    Fertility
    fertility after larval injection decreased (no living offspring) -
    iBeetle screen iB_03494

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection



    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032339 RA
      MWSYVMAAA RGDPRRRPG SVFTVQSAN CGPGPCRDG 
      LNCARYPLN SGYNSAAFQ KDGISRGFS RDELSTTIS 
      RDGSRDVLA EVRVQTPYS ERCQSPAES PFTQPLQEN 
      VPQNRLFSA MRGRLRQVA ASLQAAIRF RRFSRRDSH 
      PPDWFVDKS HQDENADAM DTPEPTCWG HRIVVDPAL 
      PSYYKWLMV VSLAVLYNV VFVLGRAVF WELNNSVPW 
      LWWTLDYFC DTIYIADIF VHAHEGYLE QGLLVTNSR 
      KLRAHYFQT KLWRSDFLA LIPTDVAYI WWPPYECNV 
      KVPCPIIVR LNRLFKLPR LWEWFERTE TDTNYPNVF 
      RICKVVLAI LVLIHWNAC LYFAISYAI GFGIDNWVY 
      NLDGPKNST LARQYIYSF YWSTLTLTT IGETPVPEN 
      DAEYLFVVA DFLAGVLIF ATIVGNIGS MISNMNVAR 
      VDFQNRMDG VKQYMAFRK VGRELEARV IRWFAYTWA 
      ESGALDEER VLSALPDKL KAEIAIRVH LETLRKVRI 
      FQDCEPGLL EALVLKLRL QVFSPGDYI CRKGDVGKE 
      MYIVKRGRL QVVADDGIT VLATLGAGS VFGEVSVLD 
      IAGNRTGNR RTANVRSLG YSDLFCLAK DDLLVALAD 
      YPEARATLT ERGCQLLRK DGLLDEDVF KKAKETQDS 
      VLDSIKKLE NTVEILQTR LARLLAEFS ASQAKLKQR 
      LTRLELSKS EREQDNTPE TLAVPTGRR RVHSFEEDR 
      IFTVAKKLD NVPFSSRHK TM
    CDS sequence
    • >TC032339 RA
      atgtggagc tatgtgatg gccgccgcc agaggtgac 
      ccacgtagg cgacctggc tccgttttc acagtacag 
      tcagccaat tgcgggcct ggaccgtgc agggacgga 
      ctgaactgt gccagatat ccgctcaat tccggatac 
      aattcggca gcgtttcaa aaggacggc ataagtcga 
      ggcttttcc agagatgaa ctcagcacg actatctcg 
      cgagacggc tccagagac gtcctggcg gaagtccgg 
      gtgcagacg ccctattcg gagagatgc caaagtccc 
      gccgagtct cccttcacg caaccactg caggaaaat 
      gtcccgcaa aacaggctg ttttcggca atgcgaggg 
      cggttaaga caagtggca gcatccctc caggcagcc 
      attcgtttc cgtcggttc tcccgaagg gactcgcac 
      cctcctgac tggttcgtg gacaaaagt caccaggat 
      gaaaatgct gatgcgatg gatactcca gaacccact 
      tgttggggt caccggatt gttgttgat ccagcgctt 
      ccgtcctac tataagtgg ctgatggtt gtctctttg 
      gctgtgctg tacaacgtt gtttttgtc ctgggaaga 
      gctgtgttt tgggaactt aataactca gtgccgtgg 
      ctttggtgg acgttggat tacttctgt gatacgatc 
      tacattgca gatatattt gttcatgct cacgaaggt 
      tatctggaa caaggactt ttggtaacg aattctcga 
      aaactgcgc gcccattac ttccaaacg aagctgtgg 
      aggagcgac tttttggcg ttgatacca acggatgtg 
      gcttacatc tggtggcct ccgtacgag tgcaacgtt 
      aaagtgccg tgtcctata attgtaaga ctgaatcga 
      ctgtttaaa ttgccgcga ctttgggaa tggttcgag 
      cggacagaa accgataca aattatccg aacgttttt 
      agaatctgt aaagtagtt ttagcaatt cttgtgctt 
      atacattgg aacgcgtgc ctctatttc gccataagc 
      tacgcgatc ggattcgga atcgacaac tgggtctac 
      aatttggat ggaccgaag aactccacg ttggctaga 
      cagtacatc tacagcttt tactggtca acgctcacc 
      ttaaccacc atcggggaa acaccagtg cctgaaaac 
      gatgccgag tatctcttt gtggttgct gactttcta 
      gccggggtc ttgatcttc gcaaccatc gtaggaaat 
      atcggttcg atgatatcg aacatgaac gtggcccgt 
      gtggatttc caaaaccga atggatggc gttaagcag 
      tacatggcc ttcaggaaa gtgggtcgc gagttggaa 
      gctagggta attcgttgg ttcgcatac acatgggca 
      gaaagcgga gctttggac gaggagcgc gtcctatcg 
      gctctacca gataaattg aaagcggaa attgctatt 
      agagtacac ttagaaaca ctccgaaaa gtaaggata 
      tttcaggac tgcgagcca ggattactg gaagctctc 
      gtgctcaaa ttacgcctg caggtgttc tccccgggt 
      gattacatt tgccgcaag ggcgacgtg ggcaaagag 
      atgtacatt gtgaaacga ggtcgatta caagtggta 
      gccgatgac ggcataact gttttagca acattaggc 
      gcaggatca gtgtttggc gaagtgagc gtcttggat 
      atcgccgga aatcgcacc ggaaacaga agaacggcc 
      aacgtccgg tcgttaggc tactcggac ctattctgc 
      ctggccaag gacgacctc ctagtcgct ttggcggat 
      tatccagag gcgagggca acgctaacc gagcgcggc 
      tgccagctt ttgcggaag gacggtcta ctggacgaa 
      gatgtgttc aaaaaggcc aaagaaact caggattcg 
      gtgctcgac agcatcaag aaattagag aatactgta 
      gaaatattg caaacacgc ctcgctaga cttcttgcc 
      gaattcagc gccagtcag gcgaagctg aagcagcga 
      ctgacccgc ttggagctc agcaagtcg gaacgtgaa 
      caagacaac acacctgag accttagca gttcctaca 
      ggacgtcga agagtgcat tcgtttgaa gaagacagg 
      atatttaca gtcgctaaa aaattagac aatgttccg 
      ttcagtagt agacataaa accatgtaa
    mRNA sequence
    • >TC032339 RA
      ccagtttca cttttcgaa atgactttt ttgaattag 
      agaagctcg atgagagcg aaaacgtgc gatgccctg 
      caatgtgga gctatgtga tggccgccg ccagaggtg 
      acccacgta ggcgacctg gctccgttt tcacagtac 
      agtcagcca attgcgggc ctggaccgt gcagggacg 
      gactgaact gtgccagat atccgctca attccggat 
      acaattcgg cagcgtttc aaaaggacg gcataagtc 
      gaggctttt ccagagatg aactcagca cgactatct 
      cgcgagacg gctccagag acgtcctgg cggaagtcc 
      gggtgcaga cgccctatt cggagagat gccaaagtc 
      ccgccgagt ctcccttca cgcaaccac tgcaggaaa 
      atgtcccgc aaaacaggc tgttttcgg caatgcgag 
      ggcggttaa gacaagtgg cagcatccc tccaggcag 
      ccattcgtt tccgtcggt tctcccgaa gggactcgc 
      accctcctg actggttcg tggacaaaa gtcaccagg 
      atgaaaatg ctgatgcga tggatactc cagaaccca 
      cttgttggg gtcaccgga ttgttgttg atccagcgc 
      ttccgtcct actataagt ggctgatgg ttgtctctt 
      tggctgtgc tgtacaacg ttgtttttg tcctgggaa 
      gagctgtgt tttgggaac ttaataact cagtgccgt 
      ggctttggt ggacgttgg attacttct gtgatacga 
      tctacattg cagatatat ttgttcatg ctcacgaag 
      gttatctgg aacaaggac ttttggtaa cgaattctc 
      gaaaactgc gcgcccatt acttccaaa cgaagctgt 
      ggaggagcg actttttgg cgttgatac caacggatg 
      tggcttaca tctggtggc ctccgtacg agtgcaacg 
      ttaaagtgc cgtgtccta taattgtaa gactgaatc 
      gactgttta aattgccgc gactttggg aatggttcg 
      agcggacag aaaccgata caaattatc cgaacgttt 
      ttagaatct gtaaagtag ttttagcaa ttcttgtgc 
      ttatacatt ggaacgcgt gcctctatt tcgccataa 
      gctacgcga tcggattcg gaatcgaca actgggtct 
      acaatttgg atggaccga agaactcca cgttggcta 
      gacagtaca tctacagct tttactggt caacgctca 
      ccttaacca ccatcgggg aaacaccag tgcctgaaa 
      acgatgccg agtatctct ttgtggttg ctgactttc 
      tagccgggg tcttgatct tcgcaacca tcgtaggaa 
      atatcggtt cgatgatat cgaacatga acgtggccc 
      gtgtggatt tccaaaacc gaatggatg gcgttaagc 
      agtacatgg ccttcagga aagtgggtc gcgagttgg 
      aagctaggg taattcgtt ggttcgcat acacatggg 
      cagaaagcg gagctttgg acgaggagc gcgtcctat 
      cggctctac cagataaat tgaaagcgg aaattgcta 
      ttagagtac acttagaaa cactccgaa aagtaagga 
      tatttcagg actgcgagc caggattac tggaagctc 
      tcgtgctca aattacgcc tgcaggtgt tctccccgg 
      gtgattaca tttgccgca agggcgacg tgggcaaag 
      agatgtaca ttgtgaaac gaggtcgat tacaagtgg 
      tagccgatg acggcataa ctgttttag caacattag 
      gcgcaggat cagtgtttg gcgaagtga gcgtcttgg 
      atatcgccg gaaatcgca ccggaaaca gaagaacgg 
      ccaacgtcc ggtcgttag gctactcgg acctattct 
      gcctggcca aggacgacc tcctagtcg ctttggcgg 
      attatccag aggcgaggg caacgctaa ccgagcgcg 
      gctgccagc ttttgcgga aggacggtc tactggacg 
      aagatgtgt tcaaaaagg ccaaagaaa ctcaggatt 
      cggtgctcg acagcatca agaaattag agaatactg 
      tagaaatat tgcaaacac gcctcgcta gacttcttg 
      ccgaattca gcgccagtc aggcgaagc tgaagcagc 
      gactgaccc gcttggagc tcagcaagt cggaacgtg 
      aacaagaca acacacctg agaccttag cagttccta 
      caggacgtc gaagagtgc attcgtttg aagaagaca 
      ggatattta cagtcgcta aaaaattag acaatgttc 
      cgttcagta gtagacata aaaccatgt aacgacata 
      aaaaagtaa tgtacataa gttttaaat ttaagcgaa 
      gctttacga gttctactg ctgtaacga tagagaaaa 
      cagtttata ttttgtcag ttttttaac taatgtagg 
      agttaagtg tttcaaaga gtaagtgac gaatatttc 
      tgtttctaa aaatagagt tacgtttcc cgtttgatt 
      aattcttat ttatatatt tttgtacac atttattta 
      aaaaccact gcaacgtac gagtataca cttaattat 
      gtgatgtaa tatagaatt aaataacga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY