General information | |||
TC032339
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 2640727..2676086 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC032339 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03493 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 6 (11 dpi)
pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt not completely molted - Number of animals showing the phenotype: 1 (16 dpi)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03494 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 9 (11 dpi)
remarks: later than schedule,
- >TC032339 RA
MWSYVMAAA RGDPRRRPG SVFTVQSAN CGPGPCRDG LNCARYPLN SGYNSAAFQ KDGISRGFS RDELSTTIS RDGSRDVLA EVRVQTPYS ERCQSPAES PFTQPLQEN VPQNRLFSA MRGRLRQVA ASLQAAIRF RRFSRRDSH PPDWFVDKS HQDENADAM DTPEPTCWG HRIVVDPAL PSYYKWLMV VSLAVLYNV VFVLGRAVF WELNNSVPW LWWTLDYFC DTIYIADIF VHAHEGYLE QGLLVTNSR KLRAHYFQT KLWRSDFLA LIPTDVAYI WWPPYECNV KVPCPIIVR LNRLFKLPR LWEWFERTE TDTNYPNVF RICKVVLAI LVLIHWNAC LYFAISYAI GFGIDNWVY NLDGPKNST LARQYIYSF YWSTLTLTT IGETPVPEN DAEYLFVVA DFLAGVLIF ATIVGNIGS MISNMNVAR VDFQNRMDG VKQYMAFRK VGRELEARV IRWFAYTWA ESGALDEER VLSALPDKL KAEIAIRVH LETLRKVRI FQDCEPGLL EALVLKLRL QVFSPGDYI CRKGDVGKE MYIVKRGRL QVVADDGIT VLATLGAGS VFGEVSVLD IAGNRTGNR RTANVRSLG YSDLFCLAK DDLLVALAD YPEARATLT ERGCQLLRK DGLLDEDVF KKAKETQDS VLDSIKKLE NTVEILQTR LARLLAEFS ASQAKLKQR LTRLELSKS EREQDNTPE TLAVPTGRR RVHSFEEDR IFTVAKKLD NVPFSSRHK TM
- >TC032339 RA
atgtggagc tatgtgatg gccgccgcc agaggtgac ccacgtagg cgacctggc tccgttttc acagtacag tcagccaat tgcgggcct ggaccgtgc agggacgga ctgaactgt gccagatat ccgctcaat tccggatac aattcggca gcgtttcaa aaggacggc ataagtcga ggcttttcc agagatgaa ctcagcacg actatctcg cgagacggc tccagagac gtcctggcg gaagtccgg gtgcagacg ccctattcg gagagatgc caaagtccc gccgagtct cccttcacg caaccactg caggaaaat gtcccgcaa aacaggctg ttttcggca atgcgaggg cggttaaga caagtggca gcatccctc caggcagcc attcgtttc cgtcggttc tcccgaagg gactcgcac cctcctgac tggttcgtg gacaaaagt caccaggat gaaaatgct gatgcgatg gatactcca gaacccact tgttggggt caccggatt gttgttgat ccagcgctt ccgtcctac tataagtgg ctgatggtt gtctctttg gctgtgctg tacaacgtt gtttttgtc ctgggaaga gctgtgttt tgggaactt aataactca gtgccgtgg ctttggtgg acgttggat tacttctgt gatacgatc tacattgca gatatattt gttcatgct cacgaaggt tatctggaa caaggactt ttggtaacg aattctcga aaactgcgc gcccattac ttccaaacg aagctgtgg aggagcgac tttttggcg ttgatacca acggatgtg gcttacatc tggtggcct ccgtacgag tgcaacgtt aaagtgccg tgtcctata attgtaaga ctgaatcga ctgtttaaa ttgccgcga ctttgggaa tggttcgag cggacagaa accgataca aattatccg aacgttttt agaatctgt aaagtagtt ttagcaatt cttgtgctt atacattgg aacgcgtgc ctctatttc gccataagc tacgcgatc ggattcgga atcgacaac tgggtctac aatttggat ggaccgaag aactccacg ttggctaga cagtacatc tacagcttt tactggtca acgctcacc ttaaccacc atcggggaa acaccagtg cctgaaaac gatgccgag tatctcttt gtggttgct gactttcta gccggggtc ttgatcttc gcaaccatc gtaggaaat atcggttcg atgatatcg aacatgaac gtggcccgt gtggatttc caaaaccga atggatggc gttaagcag tacatggcc ttcaggaaa gtgggtcgc gagttggaa gctagggta attcgttgg ttcgcatac acatgggca gaaagcgga gctttggac gaggagcgc gtcctatcg gctctacca gataaattg aaagcggaa attgctatt agagtacac ttagaaaca ctccgaaaa gtaaggata tttcaggac tgcgagcca ggattactg gaagctctc gtgctcaaa ttacgcctg caggtgttc tccccgggt gattacatt tgccgcaag ggcgacgtg ggcaaagag atgtacatt gtgaaacga ggtcgatta caagtggta gccgatgac ggcataact gttttagca acattaggc gcaggatca gtgtttggc gaagtgagc gtcttggat atcgccgga aatcgcacc ggaaacaga agaacggcc aacgtccgg tcgttaggc tactcggac ctattctgc ctggccaag gacgacctc ctagtcgct ttggcggat tatccagag gcgagggca acgctaacc gagcgcggc tgccagctt ttgcggaag gacggtcta ctggacgaa gatgtgttc aaaaaggcc aaagaaact caggattcg gtgctcgac agcatcaag aaattagag aatactgta gaaatattg caaacacgc ctcgctaga cttcttgcc gaattcagc gccagtcag gcgaagctg aagcagcga ctgacccgc ttggagctc agcaagtcg gaacgtgaa caagacaac acacctgag accttagca gttcctaca ggacgtcga agagtgcat tcgtttgaa gaagacagg atatttaca gtcgctaaa aaattagac aatgttccg ttcagtagt agacataaa accatgtaa
- >TC032339 RA
ccagtttca cttttcgaa atgactttt ttgaattag agaagctcg atgagagcg aaaacgtgc gatgccctg caatgtgga gctatgtga tggccgccg ccagaggtg acccacgta ggcgacctg gctccgttt tcacagtac agtcagcca attgcgggc ctggaccgt gcagggacg gactgaact gtgccagat atccgctca attccggat acaattcgg cagcgtttc aaaaggacg gcataagtc gaggctttt ccagagatg aactcagca cgactatct cgcgagacg gctccagag acgtcctgg cggaagtcc gggtgcaga cgccctatt cggagagat gccaaagtc ccgccgagt ctcccttca cgcaaccac tgcaggaaa atgtcccgc aaaacaggc tgttttcgg caatgcgag ggcggttaa gacaagtgg cagcatccc tccaggcag ccattcgtt tccgtcggt tctcccgaa gggactcgc accctcctg actggttcg tggacaaaa gtcaccagg atgaaaatg ctgatgcga tggatactc cagaaccca cttgttggg gtcaccgga ttgttgttg atccagcgc ttccgtcct actataagt ggctgatgg ttgtctctt tggctgtgc tgtacaacg ttgtttttg tcctgggaa gagctgtgt tttgggaac ttaataact cagtgccgt ggctttggt ggacgttgg attacttct gtgatacga tctacattg cagatatat ttgttcatg ctcacgaag gttatctgg aacaaggac ttttggtaa cgaattctc gaaaactgc gcgcccatt acttccaaa cgaagctgt ggaggagcg actttttgg cgttgatac caacggatg tggcttaca tctggtggc ctccgtacg agtgcaacg ttaaagtgc cgtgtccta taattgtaa gactgaatc gactgttta aattgccgc gactttggg aatggttcg agcggacag aaaccgata caaattatc cgaacgttt ttagaatct gtaaagtag ttttagcaa ttcttgtgc ttatacatt ggaacgcgt gcctctatt tcgccataa gctacgcga tcggattcg gaatcgaca actgggtct acaatttgg atggaccga agaactcca cgttggcta gacagtaca tctacagct tttactggt caacgctca ccttaacca ccatcgggg aaacaccag tgcctgaaa acgatgccg agtatctct ttgtggttg ctgactttc tagccgggg tcttgatct tcgcaacca tcgtaggaa atatcggtt cgatgatat cgaacatga acgtggccc gtgtggatt tccaaaacc gaatggatg gcgttaagc agtacatgg ccttcagga aagtgggtc gcgagttgg aagctaggg taattcgtt ggttcgcat acacatggg cagaaagcg gagctttgg acgaggagc gcgtcctat cggctctac cagataaat tgaaagcgg aaattgcta ttagagtac acttagaaa cactccgaa aagtaagga tatttcagg actgcgagc caggattac tggaagctc tcgtgctca aattacgcc tgcaggtgt tctccccgg gtgattaca tttgccgca agggcgacg tgggcaaag agatgtaca ttgtgaaac gaggtcgat tacaagtgg tagccgatg acggcataa ctgttttag caacattag gcgcaggat cagtgtttg gcgaagtga gcgtcttgg atatcgccg gaaatcgca ccggaaaca gaagaacgg ccaacgtcc ggtcgttag gctactcgg acctattct gcctggcca aggacgacc tcctagtcg ctttggcgg attatccag aggcgaggg caacgctaa ccgagcgcg gctgccagc ttttgcgga aggacggtc tactggacg aagatgtgt tcaaaaagg ccaaagaaa ctcaggatt cggtgctcg acagcatca agaaattag agaatactg tagaaatat tgcaaacac gcctcgcta gacttcttg ccgaattca gcgccagtc aggcgaagc tgaagcagc gactgaccc gcttggagc tcagcaagt cggaacgtg aacaagaca acacacctg agaccttag cagttccta caggacgtc gaagagtgc attcgtttg aagaagaca ggatattta cagtcgcta aaaaattag acaatgttc cgttcagta gtagacata aaaccatgt aacgacata aaaaagtaa tgtacataa gttttaaat ttaagcgaa gctttacga gttctactg ctgtaacga tagagaaaa cagtttata ttttgtcag ttttttaac taatgtagg agttaagtg tttcaaaga gtaagtgac gaatatttc tgtttctaa aaatagagt tacgtttcc cgtttgatt aattcttat ttatatatt tttgtacac atttattta aaaaccact gcaacgtac gagtataca cttaattat gtgatgtaa tatagaatt aaataacga
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
