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Gene information
General information
TC032339
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003075,TC003076 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 1562216..1597575
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032339
    iBeetle screen iB_03493
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt not completely molted - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    iBeetle screen iB_03494
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032339 RA
      MWSYVMAAA RGDPRRRPG SVFTVQSAN CGPGPCRDG 
LNCARYPLN SGYNSAAFQ KDGISRGFS RDELSTTIS 
RDGSRDVLA EVRVQTPYS ERCQSPAES PFTQPLQEN 
VPQNRLFSA MRGRLRQVA ASLQAAIRF RRFSRRDSH 
PPDWFVDKS HQDENADAM DTPEPTCWG HRIVVDPAL 
PSYYKWLMV VSLAVLYNV VFVLGRAVF WELNNSVPW 
LWWTLDYFC DTIYIADIF VHAHEGYLE QGLLVTNSR 
KLRAHYFQT KLWRSDFLA LIPTDVAYI WWPPYECNV 
KVPCPIIVR LNRLFKLPR LWEWFERTE TDTNYPNVF 
RICKVVLAI LVLIHWNAC LYFAISYAI GFGIDNWVY 
NLDGPKNST LARQYIYSF YWSTLTLTT IGETPVPEN 
DAEYLFVVA DFLAGVLIF ATIVGNIGS MISNMNVAR 
VDFQNRMDG VKQYMAFRK VGRELEARV IRWFAYTWA 
ESGALDEER VLSALPDKL KAEIAIRVH LETLRKVRI 
FQDCEPGLL EALVLKLRL QVFSPGDYI CRKGDVGKE 
MYIVKRGRL QVVADDGIT VLATLGAGS VFGEVSVLD 
IAGNRTGNR RTANVRSLG YSDLFCLAK DDLLVALAD 
YPEARATLT ERGCQLLRK DGLLDEDVF KKAKETQDS 
VLDSIKKLE NTVEILQTR LARLLAEFS ASQAKLKQR 
LTRLELSKS EREQDNTPE TLAVPTGRR RVHSFEEDR 
IFTVAKKLD NVPFSSRHK TM
    CDS sequence
    • >TC032339 RA
      atgtggagc tatgtgatg gccgccgcc agaggtgac 
ccacgtagg cgacctggc tccgttttc acagtacag 
tcagccaat tgcgggcct ggaccgtgc agggacgga 
ctgaactgt gccagatat ccgctcaat tccggatac 
aattcggca gcgtttcaa aaggacggc ataagtcga 
ggcttttcc agagatgaa ctcagcacg actatctcg 
cgagacggc tccagagac gtcctggcg gaagtccgg 
gtgcagacg ccctattcg gagagatgc caaagtccc 
gccgagtct cccttcacg caaccactg caggaaaat 
gtcccgcaa aacaggctg ttttcggca atgcgaggg 
cggttaaga caagtggca gcatccctc caggcagcc 
attcgtttc cgtcggttc tcccgaagg gactcgcac 
cctcctgac tggttcgtg gacaaaagt caccaggat 
gaaaatgct gatgcgatg gatactcca gaacccact 
tgttggggt caccggatt gttgttgat ccagcgctt 
ccgtcctac tataagtgg ctgatggtt gtctctttg 
gctgtgctg tacaacgtt gtttttgtc ctgggaaga 
gctgtgttt tgggaactt aataactca gtgccgtgg 
ctttggtgg acgttggat tacttctgt gatacgatc 
tacattgca gatatattt gttcatgct cacgaaggt 
tatctggaa caaggactt ttggtaacg aattctcga 
aaactgcgc gcccattac ttccaaacg aagctgtgg 
aggagcgac tttttggcg ttgatacca acggatgtg 
gcttacatc tggtggcct ccgtacgag tgcaacgtt 
aaagtgccg tgtcctata attgtaaga ctgaatcga 
ctgtttaaa ttgccgcga ctttgggaa tggttcgag 
cggacagaa accgataca aattatccg aacgttttt 
agaatctgt aaagtagtt ttagcaatt cttgtgctt 
atacattgg aacgcgtgc ctctatttc gccataagc 
tacgcgatc ggattcgga atcgacaac tgggtctac 
aatttggat ggaccgaag aactccacg ttggctaga 
cagtacatc tacagcttt tactggtca acgctcacc 
ttaaccacc atcggggaa acaccagtg cctgaaaac 
gatgccgag tatctcttt gtggttgct gactttcta 
gccggggtc ttgatcttc gcaaccatc gtaggaaat 
atcggttcg atgatatcg aacatgaac gtggcccgt 
gtggatttc caaaaccga atggatggc gttaagcag 
tacatggcc ttcaggaaa gtgggtcgc gagttggaa 
gctagggta attcgttgg ttcgcatac acatgggca 
gaaagcgga gctttggac gaggagcgc gtcctatcg 
gctctacca gataaattg aaagcggaa attgctatt 
agagtacac ttagaaaca ctccgaaaa gtaaggata 
tttcaggac tgcgagcca ggattactg gaagctctc 
gtgctcaaa ttacgcctg caggtgttc tccccgggt 
gattacatt tgccgcaag ggcgacgtg ggcaaagag 
atgtacatt gtgaaacga ggtcgatta caagtggta 
gccgatgac ggcataact gttttagca acattaggc 
gcaggatca gtgtttggc gaagtgagc gtcttggat 
atcgccgga aatcgcacc ggaaacaga agaacggcc 
aacgtccgg tcgttaggc tactcggac ctattctgc 
ctggccaag gacgacctc ctagtcgct ttggcggat 
tatccagag gcgagggca acgctaacc gagcgcggc 
tgccagctt ttgcggaag gacggtcta ctggacgaa 
gatgtgttc aaaaaggcc aaagaaact caggattcg 
gtgctcgac agcatcaag aaattagag aatactgta 
gaaatattg caaacacgc ctcgctaga cttcttgcc 
gaattcagc gccagtcag gcgaagctg aagcagcga 
ctgacccgc ttggagctc agcaagtcg gaacgtgaa 
caagacaac acacctgag accttagca gttcctaca 
ggacgtcga agagtgcat tcgtttgaa gaagacagg 
atatttaca gtcgctaaa aaattagac aatgttccg 
ttcagtagt agacataaa accatgtaa
    mRNA sequence
    • >TC032339 RA
      ccagtttca cttttcgaa atgactttt ttgaattag 
agaagctcg atgagagcg aaaacgtgc gatgccctg 
caatgtgga gctatgtga tggccgccg ccagaggtg 
acccacgta ggcgacctg gctccgttt tcacagtac 
agtcagcca attgcgggc ctggaccgt gcagggacg 
gactgaact gtgccagat atccgctca attccggat 
acaattcgg cagcgtttc aaaaggacg gcataagtc 
gaggctttt ccagagatg aactcagca cgactatct 
cgcgagacg gctccagag acgtcctgg cggaagtcc 
gggtgcaga cgccctatt cggagagat gccaaagtc 
ccgccgagt ctcccttca cgcaaccac tgcaggaaa 
atgtcccgc aaaacaggc tgttttcgg caatgcgag 
ggcggttaa gacaagtgg cagcatccc tccaggcag 
ccattcgtt tccgtcggt tctcccgaa gggactcgc 
accctcctg actggttcg tggacaaaa gtcaccagg 
atgaaaatg ctgatgcga tggatactc cagaaccca 
cttgttggg gtcaccgga ttgttgttg atccagcgc 
ttccgtcct actataagt ggctgatgg ttgtctctt 
tggctgtgc tgtacaacg ttgtttttg tcctgggaa 
gagctgtgt tttgggaac ttaataact cagtgccgt 
ggctttggt ggacgttgg attacttct gtgatacga 
tctacattg cagatatat ttgttcatg ctcacgaag 
gttatctgg aacaaggac ttttggtaa cgaattctc 
gaaaactgc gcgcccatt acttccaaa cgaagctgt 
ggaggagcg actttttgg cgttgatac caacggatg 
tggcttaca tctggtggc ctccgtacg agtgcaacg 
ttaaagtgc cgtgtccta taattgtaa gactgaatc 
gactgttta aattgccgc gactttggg aatggttcg 
agcggacag aaaccgata caaattatc cgaacgttt 
ttagaatct gtaaagtag ttttagcaa ttcttgtgc 
ttatacatt ggaacgcgt gcctctatt tcgccataa 
gctacgcga tcggattcg gaatcgaca actgggtct 
acaatttgg atggaccga agaactcca cgttggcta 
gacagtaca tctacagct tttactggt caacgctca 
ccttaacca ccatcgggg aaacaccag tgcctgaaa 
acgatgccg agtatctct ttgtggttg ctgactttc 
tagccgggg tcttgatct tcgcaacca tcgtaggaa 
atatcggtt cgatgatat cgaacatga acgtggccc 
gtgtggatt tccaaaacc gaatggatg gcgttaagc 
agtacatgg ccttcagga aagtgggtc gcgagttgg 
aagctaggg taattcgtt ggttcgcat acacatggg 
cagaaagcg gagctttgg acgaggagc gcgtcctat 
cggctctac cagataaat tgaaagcgg aaattgcta 
ttagagtac acttagaaa cactccgaa aagtaagga 
tatttcagg actgcgagc caggattac tggaagctc 
tcgtgctca aattacgcc tgcaggtgt tctccccgg 
gtgattaca tttgccgca agggcgacg tgggcaaag 
agatgtaca ttgtgaaac gaggtcgat tacaagtgg 
tagccgatg acggcataa ctgttttag caacattag 
gcgcaggat cagtgtttg gcgaagtga gcgtcttgg 
atatcgccg gaaatcgca ccggaaaca gaagaacgg 
ccaacgtcc ggtcgttag gctactcgg acctattct 
gcctggcca aggacgacc tcctagtcg ctttggcgg 
attatccag aggcgaggg caacgctaa ccgagcgcg 
gctgccagc ttttgcgga aggacggtc tactggacg 
aagatgtgt tcaaaaagg ccaaagaaa ctcaggatt 
cggtgctcg acagcatca agaaattag agaatactg 
tagaaatat tgcaaacac gcctcgcta gacttcttg 
ccgaattca gcgccagtc aggcgaagc tgaagcagc 
gactgaccc gcttggagc tcagcaagt cggaacgtg 
aacaagaca acacacctg agaccttag cagttccta 
caggacgtc gaagagtgc attcgtttg aagaagaca 
ggatattta cagtcgcta aaaaattag acaatgttc 
cgttcagta gtagacata aaaccatgt aacgacata 
aaaaagtaa tgtacataa gttttaaat ttaagcgaa 
gctttacga gttctactg ctgtaacga tagagaaaa 
cagtttata ttttgtcag ttttttaac taatgtagg 
agttaagtg tttcaaaga gtaagtgac gaatatttc 
tgtttctaa aaatagagt tacgtttcc cgtttgatt 
aattcttat ttatatatt tttgtacac atttattta 
aaaaccact gcaacgtac gagtataca cttaattat 
gtgatgtaa tatagaatt aaataacga