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Gene information
General information
TC032343
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003069
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 2780030..2794364
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032343
    iBeetle screen iB_03492

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    head partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva cuticularisation decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Comment: Appendages are shorter and broader.
    larva segment partially fused - Penetrance (percentage of animals/eggs on slide): more than 80%
    Comment: appears like a segmentpolarity-phenotype    
    head partiallyanteroposterior-axis not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen mostlynot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    remarks: animals lost,
    Adult morphology
    wings not closed - Number of animals showing the phenotype: 4


    wings surface irregular - Number of animals showing the phenotype: 4


    remarks: animals lost,
    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032343 RA
      MDNTTVALT ERLQSANGS TRRTSPIPT MSPAGRPSS 
      LMDTHNQSV VVVQVVVNR DERGYGMKV SGDNPVYVQ 
      SVKEGGAAE KAGLHAGDK IIKVNDVNV ISSKHTDVV 
      DLIRSSSQV VLTVQQRTV SQKGMSSPS VHNRPLTTP 
      SRITGPQPV DHEKQYQLQ LEKEQHYRL MIEKEQRYI 
      DLLRSQIAS SPDEKKYSE LSKTEKNLQ TLQAMLLRS 
      QNEQSPPLH SPLTPLSTS LPRKSRASP NGNSEPQSP 
      PPLPKRNQR PSQVDTLNN RHPPYVNGK IVTDTNALF 
      LNNEINANL RQVEEIGVR RPSRSKFHS HSEETHLVD 
      NPLYSDHPP PLPPRAPLS TNNTNESDA VNSINKQMS 
      YPLVATCAT LVNDYLPNP THHRTKSSP ESLSAMSAG 
      EASRKLIAS ESMNDLRQE GWDPADTPP GTPPPPYPS 
      PTASRRRQY GSGESGENT FEETAGDCS SPDVSPFRG 
      TNRIIANSS PIHAATPQT TQQPIISME DDEISDQES 
      SQLEDHGYF KSLSRLWER LPHLAVFMN YILSNSDPN 
      SLLFYLLTD LYKEGNAKE MRKWAFEIH SCFLVPGAP 
      LRLGNVDEN IAREIDDVL TREFDKEEI LRKIFWKAR 
      SRAKEELTK QLVDFQQKR TAGLGTIFG PTDQVLSEI 
      YNDKTKEVK LYESLFLEK LEPFLEEIE KENYDPRRY 
      YTAAALTTV LVRIFGIRP ASHTIDRCP TFVNKEKSF 
      RTKFIGRYS RKLNVQGHQ YVAQQYYTV IVCNNCHQI 
      IYGIGPQGY QCSVCLINL HRPCVKLYD DSCPGPINK 
      KDRGLGKFI RMRHENNDN RRKHSSHFI LMERERRQA 
      EEKDNSFEL NESVPFVNG PYIGPLCNS RKLDIPAHS 
      PPEPCAFSL PVTDYTRTG TRTKSAVLF ECLLRLG
    • >TC032343 RB
      MCSVGKMDN TTVALTERL QSANGSTRR TSPIPTMSP 
      AGRPSSLMD THNQSVVVV QVVVNRDER GYGMKVSGD 
      NPVYVQSVK EGGAAEKAG LHAGDKIIK VNDVNVISS 
      KHTDVVDLI RSSSQVVLT VQQRTVSQK GMSSPSVHN 
      RPLTTPSRI TGPQPVDHE KQYQLQLEK EQHYRLMIE 
      KEQRYIDLL RSQIASSPD EKKYSELSK TEKNLQTLQ 
      AMLLRSQNE QSPPLHSPL TPLSTSLPR KSRASPNGN 
      SEPQSPPPL PKRNQRPSQ VDTLNNRHP PYVNGKIVT 
      DTNALFLNN EINANLRQV EEIGVRRPS RSKFHSHSE 
      ETHLVDNPL YSDHPPPLP PRAPLSTNN TNESDAVNS 
      INKQMSYPL VATCATLVN DYLPNPTHH RTKSSPESL 
      SAMSAGEAS RKLIASESM NDLRQEGWD PADTPPGTP 
      PPPYPSPTA SRRRQYGSG ESGENTFEE TAGDCSSPD 
      VSPFRGTNR IIANSSPIH AATPQTTQQ PIISMEDDE 
      ISDQESSQL EDHGYFKSL SRLWERLPH LAVFMNYIL 
      SNSDPNSLL FYLLTDLYK EGNAKEMRK WAFEIHSCF 
      LVPGAPLRL GNVDENIAR EIDDVLTRE FDKEEILRK 
      IFWKARSRA KEELTKQLV DFQQKRTAG LGTIFGPTD 
      QVLSEIYND KTKEVKLYE SLFLEKLEP FLEEIEKEN 
      YDPRRYYTA AALTTVLVR IFGIRPASH TIDRCPTFV 
      NKEKSFRTK FIGRYSRKL NVQGHQYVA QQYYTVIVC 
      NNCHQIIYG IGPQGYQCS VCLINLHRP CVKLYDDSC 
      PGPINKKDR GLGKFIRMR HENNDNRRK HSSHFILME 
      RERRQAEEK DNSFELNES VPFVNGPYI GPLCNSRKL 
      DIPAHSPPE PCAFSLPVT DYTRTGTRT KSAVLFECL 
      LRLG
    CDS sequence
    • >TC032343 RA
      atggataat acgacggtt gccttaact gaaagactt 
      caatcagca aatggatca acccggagg acgtctcca 
      atacccacc atgtctcct gcgggcaga ccctcatct 
      ctcatggat actcataat cagtcagtt gttgttgtc 
      caggtagtt gttaatcgc gacgaacga ggatatggc 
      atgaaggtt tcgggtgat aacccagta tacgtacaa 
      agtgttaaa gagggagga gctgccgaa aaagcgggc 
      ttgcacgct ggtgataaa ataatcaag gtcaatgat 
      gttaacgtg atatcgtcc aaacacacg gatgtggtc 
      gatttaata agatcttcg tcccaagtg gtgttaaca 
      gtccagcaa aggactgtt tcgcaaaaa gggatgagt 
      tctcccagt gtgcacaac cgacccctg acgactccg 
      tccagaatt accggacct cagccggtt gatcatgag 
      aagcaatac cagctccag ttggagaag gagcagcat 
      tatagactg atgattgag aaagaacag cgatacatc 
      gatttgctt aggagtcag atagcgtca tctcccgat 
      gagaagaag tactcggag ctgtccaag acagagaag 
      aatctgcag acgctccaa gctatgctc cttcgcagc 
      caaaacgag cagtctcca cctttacac tctccccta 
      actcccttg tcaacctcc ttacctcgc aagagtaga 
      gcgtctccc aacggcaat tcagagccg caatcgccg 
      ccaccgttg ccgaagaga aaccaacga ccgtcccag 
      gtggacact ttaaataac cgacaccct ccatacgtg 
      aatggcaaa atagtcact gatactaac gctttgttt 
      cttaacaac gaaataaac gccaatctg cgacaggtc 
      gaagagatt ggtgttcga cggccgtct agaagcaag 
      ttccactcg cattcggaa gagacacat ctcgttgac 
      aaccctctc tactcagac caccctcct cctttacct 
      cccagagcc cccctttcc acaaacaat acaaacgaa 
      tcggatgct gttaactcc ataaataag caaatgtcg 
      tatccctta gtggcaacc tgtgcgacg ttagttaat 
      gattatttg ccaaatcca acgcatcat cgcacaaaa 
      tctagtcct gaatctcta tcagcaatg tcggctggt 
      gaagcatca cggaaactg atagcttcc gaaagcatg 
      aacgatctg aggcaagaa gggtgggac cctgctgac 
      acaccacct ggaacacca ccgccacca tatcccagt 
      cctacggct tccaggaga agacagtat gggagtggt 
      gaaagcgga gaaaataca tttgaagaa acggctggt 
      gattgttcc tcgccggat gtttcaccg tttcgggga 
      accaatcgc attatcgcg aatagttca ccgattcat 
      gctgcaacg cctcaaacc acgcagcag ccaatcata 
      agcatggaa gatgacgaa atctcagat caggaatct 
      agccagttg gaagatcac ggctatttc aaatcgctc 
      tctagactc tgggagcgt ctaccacat ttggctgtg 
      ttcatgaat tatatttta tcgaattcg gatccgaat 
      agtcttctg ttttatttg ctcactgat ttgtataaa 
      gaagggaac gcgaaggag atgaggaag tgggctttt 
      gaaatacat tcgtgtttc ctcgtccct ggagcgcct 
      ttacgtttg ggaaatgtg gacgagaac atcgctcgc 
      gaaatcgac gacgttctc acacgcgag tttgataaa 
      gaggaaatt cttaggaaa attttttgg aaggcaaga 
      tctcgggcc aaagaagaa ctaacgaag caattggtt 
      gatttccag cagaaacgc actgcaggt ttaggcaca 
      atttttggc ccaactgac caagtcctg tccgaaatt 
      tacaacgat aagaccaaa gaagtgaaa ttgtacgag 
      agtttattt ttagaaaag ttggaacct tttttagaa 
      gaaattgag aaagagaat tacgatccg cgtcggtat 
      tacacagca gcagctctg acgacggtt ttagttcgc 
      atctttggt attcggccg gcttcgcat accatcgat 
      cgctgtcca acttttgta aacaaggag aagtcgttc 
      cgcacgaaa tttattgga agatattcc cgaaagtta 
      aacgttcaa ggacaccaa tacgtcgca caacaatat 
      tatactgtg atagtttgt aataattgc catcaaatt 
      atttatgga ataggacct caaggctat cagtgttct 
      gtttgtttg attaatctt caccgtccc tgtgtcaag 
      ttgtatgac gacagttgt ccaggtcca atcaataaa 
      aaggatcgg ggcttagga aaatttata cggatgaga 
      cacgagaat aatgacaat aggcgaaaa cacagctct 
      cattttata ttaatggaa agggaaagg aggcaagcc 
      gaggaaaaa gacaacagt ttcgagctc aacgaaagt 
      gtgcccttt gtcaacggt ccctatatt gggccttta 
      tgcaattca cgaaaattg gatataccg gcgcatagt 
      ccaccggag ccctgcgcg ttctcgttg ccagtcaca 
      gattacacg cggacggga acgagaacg aaaagtgcg 
      gtgttattt gagtgcctg cttaggtta ggttag
    • >TC032343 RB
      atgtgttct gttggcaaa atggataat acgacggtt 
      gccttaact gaaagactt caatcagca aatggatca 
      acccggagg acgtctcca atacccacc atgtctcct 
      gcgggcaga ccctcatct ctcatggat actcataat 
      cagtcagtt gttgttgtc caggtagtt gttaatcgc 
      gacgaacga ggatatggc atgaaggtt tcgggtgat 
      aacccagta tacgtacaa agtgttaaa gagggagga 
      gctgccgaa aaagcgggc ttgcacgct ggtgataaa 
      ataatcaag gtcaatgat gttaacgtg atatcgtcc 
      aaacacacg gatgtggtc gatttaata agatcttcg 
      tcccaagtg gtgttaaca gtccagcaa aggactgtt 
      tcgcaaaaa gggatgagt tctcccagt gtgcacaac 
      cgacccctg acgactccg tccagaatt accggacct 
      cagccggtt gatcatgag aagcaatac cagctccag 
      ttggagaag gagcagcat tatagactg atgattgag 
      aaagaacag cgatacatc gatttgctt aggagtcag 
      atagcgtca tctcccgat gagaagaag tactcggag 
      ctgtccaag acagagaag aatctgcag acgctccaa 
      gctatgctc cttcgcagc caaaacgag cagtctcca 
      cctttacac tctccccta actcccttg tcaacctcc 
      ttacctcgc aagagtaga gcgtctccc aacggcaat 
      tcagagccg caatcgccg ccaccgttg ccgaagaga 
      aaccaacga ccgtcccag gtggacact ttaaataac 
      cgacaccct ccatacgtg aatggcaaa atagtcact 
      gatactaac gctttgttt cttaacaac gaaataaac 
      gccaatctg cgacaggtc gaagagatt ggtgttcga 
      cggccgtct agaagcaag ttccactcg cattcggaa 
      gagacacat ctcgttgac aaccctctc tactcagac 
      caccctcct cctttacct cccagagcc cccctttcc 
      acaaacaat acaaacgaa tcggatgct gttaactcc 
      ataaataag caaatgtcg tatccctta gtggcaacc 
      tgtgcgacg ttagttaat gattatttg ccaaatcca 
      acgcatcat cgcacaaaa tctagtcct gaatctcta 
      tcagcaatg tcggctggt gaagcatca cggaaactg 
      atagcttcc gaaagcatg aacgatctg aggcaagaa 
      gggtgggac cctgctgac acaccacct ggaacacca 
      ccgccacca tatcccagt cctacggct tccaggaga 
      agacagtat gggagtggt gaaagcgga gaaaataca 
      tttgaagaa acggctggt gattgttcc tcgccggat 
      gtttcaccg tttcgggga accaatcgc attatcgcg 
      aatagttca ccgattcat gctgcaacg cctcaaacc 
      acgcagcag ccaatcata agcatggaa gatgacgaa 
      atctcagat caggaatct agccagttg gaagatcac 
      ggctatttc aaatcgctc tctagactc tgggagcgt 
      ctaccacat ttggctgtg ttcatgaat tatatttta 
      tcgaattcg gatccgaat agtcttctg ttttatttg 
      ctcactgat ttgtataaa gaagggaac gcgaaggag 
      atgaggaag tgggctttt gaaatacat tcgtgtttc 
      ctcgtccct ggagcgcct ttacgtttg ggaaatgtg 
      gacgagaac atcgctcgc gaaatcgac gacgttctc 
      acacgcgag tttgataaa gaggaaatt cttaggaaa 
      attttttgg aaggcaaga tctcgggcc aaagaagaa 
      ctaacgaag caattggtt gatttccag cagaaacgc 
      actgcaggt ttaggcaca atttttggc ccaactgac 
      caagtcctg tccgaaatt tacaacgat aagaccaaa 
      gaagtgaaa ttgtacgag agtttattt ttagaaaag 
      ttggaacct tttttagaa gaaattgag aaagagaat 
      tacgatccg cgtcggtat tacacagca gcagctctg 
      acgacggtt ttagttcgc atctttggt attcggccg 
      gcttcgcat accatcgat cgctgtcca acttttgta 
      aacaaggag aagtcgttc cgcacgaaa tttattgga 
      agatattcc cgaaagtta aacgttcaa ggacaccaa 
      tacgtcgca caacaatat tatactgtg atagtttgt 
      aataattgc catcaaatt atttatgga ataggacct 
      caaggctat cagtgttct gtttgtttg attaatctt 
      caccgtccc tgtgtcaag ttgtatgac gacagttgt 
      ccaggtcca atcaataaa aaggatcgg ggcttagga 
      aaatttata cggatgaga cacgagaat aatgacaat 
      aggcgaaaa cacagctct cattttata ttaatggaa 
      agggaaagg aggcaagcc gaggaaaaa gacaacagt 
      ttcgagctc aacgaaagt gtgcccttt gtcaacggt 
      ccctatatt gggccttta tgcaattca cgaaaattg 
      gatataccg gcgcatagt ccaccggag ccctgcgcg 
      ttctcgttg ccagtcaca gattacacg cggacggga 
      acgagaacg aaaagtgcg gtgttattt gagtgcctg 
      cttaggtta ggttag
    mRNA sequence
    • >TC032343 RA
      ttataattc tagacgcgt atactgtga tggtgctgt 
      tgttgcata attttttgt tactagaat tttaaagat 
      cgttcactg aagaccagt cagttgatt agatttact 
      gataacatg cagcatgat ggttctcaa aacttgttt 
      tattttatt actgttgag ttttggttt agtgcttac 
      gcagaataa cagtattga cagaatttg caaaaattg 
      tggtgatat cgtcaatag atattgctg cagatacca 
      gaaacgatg ttgtgaatg gcaaaatgg ataatacga 
      cggttgcct taactgaaa gacttcaat cagcaaatg 
      gatcaaccc ggaggacgt ctccaatac ccaccatgt 
      ctcctgcgg gcagaccct catctctca tggatactc 
      ataatcagt cagttgttg ttgtccagg tagttgtta 
      atcgcgacg aacgaggat atggcatga aggtttcgg 
      gtgataacc cagtatacg tacaaagtg ttaaagagg 
      gaggagctg ccgaaaaag cgggcttgc acgctggtg 
      ataaaataa tcaaggtca atgatgtta acgtgatat 
      cgtccaaac acacggatg tggtcgatt taataagat 
      cttcgtccc aagtggtgt taacagtcc agcaaagga 
      ctgtttcgc aaaaaggga tgagttctc ccagtgtgc 
      acaaccgac ccctgacga ctccgtcca gaattaccg 
      gacctcagc cggttgatc atgagaagc aataccagc 
      tccagttgg agaaggagc agcattata gactgatga 
      ttgagaaag aacagcgat acatcgatt tgcttagga 
      gtcagatag cgtcatctc ccgatgaga agaagtact 
      cggagctgt ccaagacag agaagaatc tgcagacgc 
      tccaagcta tgctccttc gcagccaaa acgagcagt 
      ctccacctt tacactctc ccctaactc ccttgtcaa 
      cctccttac ctcgcaaga gtagagcgt ctcccaacg 
      gcaattcag agccgcaat cgccgccac cgttgccga 
      agagaaacc aacgaccgt cccaggtgg acactttaa 
      ataaccgac accctccat acgtgaatg gcaaaatag 
      tcactgata ctaacgctt tgtttctta acaacgaaa 
      taaacgcca atctgcgac aggtcgaag agattggtg 
      ttcgacggc cgtctagaa gcaagttcc actcgcatt 
      cggaagaga cacatctcg ttgacaacc ctctctact 
      cagaccacc ctcctcctt tacctccca gagcccccc 
      tttccacaa acaatacaa acgaatcgg atgctgtta 
      actccataa ataagcaaa tgtcgtatc ccttagtgg 
      caacctgtg cgacgttag ttaatgatt atttgccaa 
      atccaacgc atcatcgca caaaatcta gtcctgaat 
      ctctatcag caatgtcgg ctggtgaag catcacgga 
      aactgatag cttccgaaa gcatgaacg atctgaggc 
      aagaagggt gggaccctg ctgacacac cacctggaa 
      caccaccgc caccatatc ccagtccta cggcttcca 
      ggagaagac agtatggga gtggtgaaa gcggagaaa 
      atacatttg aagaaacgg ctggtgatt gttcctcgc 
      cggatgttt caccgtttc ggggaacca atcgcatta 
      tcgcgaata gttcaccga ttcatgctg caacgcctc 
      aaaccacgc agcagccaa tcataagca tggaagatg 
      acgaaatct cagatcagg aatctagcc agttggaag 
      atcacggct atttcaaat cgctctcta gactctggg 
      agcgtctac cacatttgg ctgtgttca tgaattata 
      ttttatcga attcggatc cgaatagtc ttctgtttt 
      atttgctca ctgatttgt ataaagaag ggaacgcga 
      aggagatga ggaagtggg cttttgaaa tacattcgt 
      gtttcctcg tccctggag cgcctttac gtttgggaa 
      atgtggacg agaacatcg ctcgcgaaa tcgacgacg 
      ttctcacac gcgagtttg ataaagagg aaattctta 
      ggaaaattt tttggaagg caagatctc gggccaaag 
      aagaactaa cgaagcaat tggttgatt tccagcaga 
      aacgcactg caggtttag gcacaattt ttggcccaa 
      ctgaccaag tcctgtccg aaatttaca acgataaga 
      ccaaagaag tgaaattgt acgagagtt tatttttag 
      aaaagttgg aaccttttt tagaagaaa ttgagaaag 
      agaattacg atccgcgtc ggtattaca cagcagcag 
      ctctgacga cggttttag ttcgcatct ttggtattc 
      ggccggctt cgcatacca tcgatcgct gtccaactt 
      ttgtaaaca aggagaagt cgttccgca cgaaattta 
      ttggaagat attcccgaa agttaaacg ttcaaggac 
      accaatacg tcgcacaac aatattata ctgtgatag 
      tttgtaata attgccatc aaattattt atggaatag 
      gacctcaag gctatcagt gttctgttt gtttgatta 
      atcttcacc gtccctgtg tcaagttgt atgacgaca 
      gttgtccag gtccaatca ataaaaagg atcggggct 
      taggaaaat ttatacgga tgagacacg agaataatg 
      acaataggc gaaaacaca gctctcatt ttatattaa 
      tggaaaggg aaaggaggc aagccgagg aaaaagaca 
      acagtttcg agctcaacg aaagtgtgc cctttgtca 
      acggtccct atattgggc ctttatgca attcacgaa 
      aattggata taccggcgc atagtccac cggagccct 
      gcgcgttct cgttgccag tcacagatt acacgcgga 
      cgggaacga gaacgaaaa gtgcggtgt tatttgagt 
      gcctgctta ggttaggtt agtgtggtg gtggtgatc 
      ccagtttgt tatgtcttg aaagctccg cacaaatcc 
      gcctgatat gccttgcgt ccttcccgc gagcttaat 
      tggcacgtt tccgcaagg ataattgca aagtgcggt 
      cggtcttcc ccagctgac aggacgaat gcttaattg 
      tatcgattc ggataattg tcttgattc aattaactt 
      cgccttgaa cgatggtgc ctacactct ttggaaatc 
      acaaactcg taaagcctc catgcaatc agcctcgta 
      attggatcc gaatgtagc aagcgaaca ccggcgcgt 
      ttttatgac aaatttgca cattttatg gaggcgata 
      gtataactc ttgttgcga tggacatta aagggttgt 
      ccactgcca attgcgcat ttttcaccg tcctgttaa 
      ccatgttaa attagcgat tgtatcacc tccgcattt 
      cctgtgggt tttgctaaa aatagcgct ttttacaat 
      atttcggca agagttcca gtttgccgt tttccttgc 
      aattatcta atcgtgcgg agacataaa tgtgcaaag 
      tgtgcggcg tgctccaaa atccagttt tatggaggc 
      acaggatta cacttctcg gtccggcag cttgaagag 
      atccagtag cttcaagaa attactcaa attgccaaa 
      ttggggttc ggggagttc aggtaaata aagtgacgt 
      cgtgatg
    • >TC032343 RB
      ttataattc tagacgcgt atactgtga tggtgctgt 
      tgttgcata attttttgt tactagaat tttaaagat 
      cgttcactg aagaccagt cagttgatt agatttact 
      gataacatg cagcatgat ggttctcaa aacttgttt 
      tattttatt actgttgag ttttggttt agtgcttac 
      gcagaataa cagtattga cagaatttg caaaaattg 
      tggtgatat cgtcaatag atattgctg cagatacca 
      gaaacgatg ttgtgaata tttaaatcc gtctggttg 
      tcctacccc agcaagtcc agaagctcg gataagttg 
      tcgaacccc ccagttttg tcggtcaag tactgattg 
      tcccatgtt taaacaatg ctgtgatac gtccatatg 
      tggagcaac atgcatgaa gccaacatt ttctggata 
      cagatattg aaaaaagtg gtttaaact ccttttttt 
      tctataatt cgtgtcagc atctgacat aaaaagcca 
      atttgtaat acgttttga taattgagt tatgctata 
      attaatagt gtcgtaatc gtaaaacat aattgatta 
      ttctaatgg gagaagcag taaaatatg gcttgtatc 
      cagagaagt tttatctcg aatctcgaa aataaatca 
      tgaattaat aaattataa ttgttttat gggtaatat 
      acctacaaa gtgaagaat accttggct gtgcttatg 
      tcatagtaa taacaaact tgacaaata taactgtga 
      taacatctt cggtgattc atttgtatt attaattac 
      gtggtatct cgcttttat ttatttata acaaacatt 
      tgatgtatt cagcggcgt gtttttgta atcgaataa 
      aaatagaaa caatttcga ttttcaaga taaatgtat 
      ctgtatttg taaattgtt tagaaagca tcgctacag 
      ctaacgcgg tatatttag catcacggg tgtaagcaa 
      agttgcttt ttgtgccca acaccataa tgttaggtt 
      tagcacaaa actgttatt tcatcggct aatgatacg 
      ctattagcc aaattaaat tcgctgtta tttctttag 
      gcatttccc tgaaaaaaa aaagttgga aaaaattcc 
      tctcccagt actccctct gcgcagaat cctcttcgg 
      gaattaccc aagatggct gacacttgt tggtcgacg 
      gagttgtga ttgagtttg cgatcgaaa aaagtgcct 
      taatcgaac cgatttcga aaatttttg gcgcgaagg 
      cgaacccga ccggtgtta gtgatatgt tgagtgcgt 
      aaaattcgt gttagtgat gttagcctt cgagaacaa 
      tcgatatgt gttctgttg gcaaaatgg ataatacga 
      cggttgcct taactgaaa gacttcaat cagcaaatg 
      gatcaaccc ggaggacgt ctccaatac ccaccatgt 
      ctcctgcgg gcagaccct catctctca tggatactc 
      ataatcagt cagttgttg ttgtccagg tagttgtta 
      atcgcgacg aacgaggat atggcatga aggtttcgg 
      gtgataacc cagtatacg tacaaagtg ttaaagagg 
      gaggagctg ccgaaaaag cgggcttgc acgctggtg 
      ataaaataa tcaaggtca atgatgtta acgtgatat 
      cgtccaaac acacggatg tggtcgatt taataagat 
      cttcgtccc aagtggtgt taacagtcc agcaaagga 
      ctgtttcgc aaaaaggga tgagttctc ccagtgtgc 
      acaaccgac ccctgacga ctccgtcca gaattaccg 
      gacctcagc cggttgatc atgagaagc aataccagc 
      tccagttgg agaaggagc agcattata gactgatga 
      ttgagaaag aacagcgat acatcgatt tgcttagga 
      gtcagatag cgtcatctc ccgatgaga agaagtact 
      cggagctgt ccaagacag agaagaatc tgcagacgc 
      tccaagcta tgctccttc gcagccaaa acgagcagt 
      ctccacctt tacactctc ccctaactc ccttgtcaa 
      cctccttac ctcgcaaga gtagagcgt ctcccaacg 
      gcaattcag agccgcaat cgccgccac cgttgccga 
      agagaaacc aacgaccgt cccaggtgg acactttaa 
      ataaccgac accctccat acgtgaatg gcaaaatag 
      tcactgata ctaacgctt tgtttctta acaacgaaa 
      taaacgcca atctgcgac aggtcgaag agattggtg 
      ttcgacggc cgtctagaa gcaagttcc actcgcatt 
      cggaagaga cacatctcg ttgacaacc ctctctact 
      cagaccacc ctcctcctt tacctccca gagcccccc 
      tttccacaa acaatacaa acgaatcgg atgctgtta 
      actccataa ataagcaaa tgtcgtatc ccttagtgg 
      caacctgtg cgacgttag ttaatgatt atttgccaa 
      atccaacgc atcatcgca caaaatcta gtcctgaat 
      ctctatcag caatgtcgg ctggtgaag catcacgga 
      aactgatag cttccgaaa gcatgaacg atctgaggc 
      aagaagggt gggaccctg ctgacacac cacctggaa 
      caccaccgc caccatatc ccagtccta cggcttcca 
      ggagaagac agtatggga gtggtgaaa gcggagaaa 
      atacatttg aagaaacgg ctggtgatt gttcctcgc 
      cggatgttt caccgtttc ggggaacca atcgcatta 
      tcgcgaata gttcaccga ttcatgctg caacgcctc 
      aaaccacgc agcagccaa tcataagca tggaagatg 
      acgaaatct cagatcagg aatctagcc agttggaag 
      atcacggct atttcaaat cgctctcta gactctggg 
      agcgtctac cacatttgg ctgtgttca tgaattata 
      ttttatcga attcggatc cgaatagtc ttctgtttt 
      atttgctca ctgatttgt ataaagaag ggaacgcga 
      aggagatga ggaagtggg cttttgaaa tacattcgt 
      gtttcctcg tccctggag cgcctttac gtttgggaa 
      atgtggacg agaacatcg ctcgcgaaa tcgacgacg 
      ttctcacac gcgagtttg ataaagagg aaattctta 
      ggaaaattt tttggaagg caagatctc gggccaaag 
      aagaactaa cgaagcaat tggttgatt tccagcaga 
      aacgcactg caggtttag gcacaattt ttggcccaa 
      ctgaccaag tcctgtccg aaatttaca acgataaga 
      ccaaagaag tgaaattgt acgagagtt tatttttag 
      aaaagttgg aaccttttt tagaagaaa ttgagaaag 
      agaattacg atccgcgtc ggtattaca cagcagcag 
      ctctgacga cggttttag ttcgcatct ttggtattc 
      ggccggctt cgcatacca tcgatcgct gtccaactt 
      ttgtaaaca aggagaagt cgttccgca cgaaattta 
      ttggaagat attcccgaa agttaaacg ttcaaggac 
      accaatacg tcgcacaac aatattata ctgtgatag 
      tttgtaata attgccatc aaattattt atggaatag 
      gacctcaag gctatcagt gttctgttt gtttgatta 
      atcttcacc gtccctgtg tcaagttgt atgacgaca 
      gttgtccag gtccaatca ataaaaagg atcggggct 
      taggaaaat ttatacgga tgagacacg agaataatg 
      acaataggc gaaaacaca gctctcatt ttatattaa 
      tggaaaggg aaaggaggc aagccgagg aaaaagaca 
      acagtttcg agctcaacg aaagtgtgc cctttgtca 
      acggtccct atattgggc ctttatgca attcacgaa 
      aattggata taccggcgc atagtccac cggagccct 
      gcgcgttct cgttgccag tcacagatt acacgcgga 
      cgggaacga gaacgaaaa gtgcggtgt tatttgagt 
      gcctgctta ggttaggtt agtgtggtg gtggtgatc 
      ccagtttgt tatgtcttg aaagctccg cacaaatcc 
      gcctgatat gccttgcgt ccttcccgc gagcttaat 
      tggcacgtt tccgcaagg ataattgca aagtgcggt 
      cggtcttcc ccagctgac aggacgaat gcttaattg 
      tatcgattc ggataattg tcttgattc aattaactt 
      cgccttgaa cgatggtgc ctacactct ttggaaatc 
      acaaactcg taaagcctc catgcaatc agcctcgta 
      attggatcc gaatgtagc aagcgaaca ccggcgcgt 
      ttttatgac aaatttgca cattttatg gaggcgata 
      gtataactc ttgttgcga tggacatta aagggttgt 
      ccactgcca attgcgcat ttttcaccg tcctgttaa 
      ccatgttaa attagcgat tgtatcacc tccgcattt 
      cctgtgggt tttgctaaa aatagcgct ttttacaat 
      atttcggca agagttcca gtttgccgt tttccttgc 
      aattatcta atcgtgcgg agacataaa tgtgcaaag 
      tgtgcggcg tgctccaaa atccagttt tatggaggc 
      acaggatta cacttctcg gtccggcag cttgaagag 
      atccagtag cttcaagaa attactcaa attgccaaa 
      ttggggttc ggggagttc aggtaaata aagtgacgt 
      cgtgatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY