Status
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Gene information
General information
TC003068 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 2801239..2809426
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003068
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03491

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003068 RA
      MFSICKQTH PATGVEHAI SCHFFSKSE KSLVTSGAN 
      VIKVFRLIP DIDTKTRID KFNETNPPK SKLECVAQY 
      TLFGNIMSM QSVNLANSP RDALLLAFK DAKLSVVEY 
      DPETHDLKT LSLHYFEED DMKDGWTHH YHVPMVRAD 
      PENRCAVMT VFGRKLVVL PFRRENAID DTDADIKPM 
      IGGAYGSKA PILASYMIV LKDFIDKVD NIIDIQFLH 
      GYYEPTLLI LFEPLKTFA GRVAVRTDT CAMAAISLN 
      LQQKVHPII WSVANLPFD CVKAVPIKK PLGGTLIFA 
      VNALIYLNQ SIPPYGVSL NSIAENSTN FPLKPQDDL 
      CISLDCAQA TFLEDDTIV LSLKGGELY VLTLLADNM 
      RYVRSFHFE KAAASVLTT CISVCENNF LFLGSRLGN 
      SLLLRFTEK CNEVITLDE TIEPSAKRL KASNSTSEN 
      EDDKVLDTL NDCMASDVL DIRDPEELE VYGNQKQAS 
      LQISSYVFE VCDSLLNIG PCGNISLGE PAFLSEEFS 
      ENLDLDLEL VTTAGYGKN GALCVLQKS VRPQIVTTF 
      TLPGCSNMW TVHAGEDKH AFLILSQED GTMILQTGD 
      EINEIDNTG FATHIPTVY AGNLGNLKY IVQVTSSAV 
      RLLQGINQL QHIPLELGS PIVHVTSVD PYISLLTTD 
      GQVITLMLR EARGVAKLV ISKSTLSNS PPVTTICMY 
      RDVSGLFTS KIPEDFTHI PEHFINESE TKMEVENED 
      DLLYGDDSD FKMPTLNPP QPKPKVYYN WWKKYLLDV 
      RPSYWLFVV RENSNLEIY SIPDFKLCY YITNLCFGH 
      KVLVDNLES VTISASTPI SAAHEANIQ RQFDVKEIL 
      VVALGNHGS RPLLMVRLE RDLYIYEVF RFPRGNLKM 
      RFRKIKHSL IYSPNVSGR IDTEDSDFF AIQERIIKM 
      RYFTNIAGY NGVFVCGAN PHWIFMSAR GELRTHPMT 
      IDGEVLSFA AFNNVNCPQ GFLYFNRKS ELRIGVLPT 
      HLSYDAAWP VRKVPLRCT PHFVTYHLE SKTYCLVTS 
      IAEPSNKYY KFNGEDKEL SVEDRGDRF PYPLQEKFS 
      LMLFSPVSW DVIPNTKID LDEWEHVNC LKNVSLAYE 
      GTRSGLKGY IAVGTNYNY GEDVTSRGR ILIFDIIEV 
      VPEPGQPLT KNRFKEIYA KDQKGPVTA LSQVKGFLV 
      SAVGQKIYI WQLKDNDLV GVAFIDTQI YTHQILTIK 
      SLLLVADVY KSISLLRFQ EEYRTLSLV SRDFRPCEV 
      FSVEYMIDN TTMGFLVSD SEKNLVLYM YQPESRESL 
      GGQRLLRKA DFHLGQAVN SFFRIKCKL GELGEDKKN 
      LTGADKRHI TMYATLDGG LGYIMPVPE KTYRRLLML 
      QNVLVSQGA HIAGLNPKA FRTYKSWKK LQTNPARSV 
      IDGELVYNY LQLSIPEKL EVSKKIGTK LEELLDDLS 
      DIQKITNHF
    CDS sequence
    • >TC003068 RA
      atgttttct atttgtaaa caaacgcac cctgccacg 
      ggggtggaa cacgcaatt tcgtgccat tttttcagc 
      aagtctgag aaaagttta gttacgtca ggggccaac 
      gtaatcaaa gtgtttcgc ctgatccca gacattgac 
      acaaaaacc agaattgat aaatttaat gaaacaaac 
      cctccgaag tctaagcta gaatgtgtg gctcaatac 
      actttgttt ggaaatatc atgtccatg caaagcgta 
      aatcttgca aattcgccc agagacgct ctgttatta 
      gcgttcaaa gacgcaaaa ttatcagtg gtggaatat 
      gaccccgaa acgcacgat ttgaagact ctgagcttg 
      cactacttt gaggaagat gacatgaag gacggttgg 
      acccaccat taccatgtg cctatggtg cgagctgat 
      cctgaaaac cgctgcgct gttatgacc gttttcggc 
      cgaaaattg gtagttctt cctttcagg cgtgaaaac 
      gcaattgat gacaccgat gctgatatt aagccaatg 
      attggtgga gcttacgga tcaaaagcg ccaatcttg 
      gcctcttat atgattgtt ttaaaggac tttatcgat 
      aaagttgac aacataata gacatccag ttcttgcac 
      ggttattac gagccaacg ctcctgatt ttgttcgag 
      cctttgaaa acttttgct gggcgcgtc gctgtgcga 
      actgatacg tgtgccatg gctgcaatt tccctcaac 
      ttgcagcaa aaagtgcac ccgattatt tggagtgtt 
      gcaaatttg ccgtttgat tgtgtaaaa gccgttcct 
      attaaaaaa ccgctcggt ggcacgttg atttttgca 
      gtaaatgca ctaatttat ctaaatcaa agtattcca 
      ccgtatggg gtttccttg aacagtatt gccgaaaat 
      tcaacaaat tttccatta aaaccgcag gacgattta 
      tgtataagt ttagattgt gcacaagcc acgtttttg 
      gaagacgat acaattgtc ttgtctctg aaagggggc 
      gaactttac gtcctaact ttgctggca gacaatatg 
      cgatatgtt aggagtttt cattttgag aaagctgca 
      gccagtgtt ctcacaaca tgtatctct gtatgtgag 
      aacaacttt ttgttcctc ggctcgcgc ctcggaaac 
      tcccttctt ttgcgcttc actgaaaaa tgcaacgaa 
      gtcataact ctggatgaa acgatcgaa ccgagcgcc 
      aaacgcctg aaagctagc aatagtaca agtgaaaat 
      gaggacgat aaagtcctg gacacgtta aacgattgt 
      atggctagt gatgtgtta gatattcgc gatccggaa 
      gaactggaa gtgtacgga aatcaaaag caagccagt 
      cttcaaata tcaagctac gtctttgaa gtctgtgat 
      agtttactc aacattggc ccttgtggc aatatctcg 
      ctaggagaa ccggctttt ctgagcgag gaattttcc 
      gaaaacctt gatttagat ttggaactt gttacaaca 
      gcagggtat gggaaaaac ggggcgctt tgtgtgtta 
      cagaagtct gtaaggccg caaattgtg accactttt 
      acgctacct gggtgctcc aatatgtgg actgtgcat 
      gccggggag gacaaacat gcgtttctc attttgagt 
      caagaggac ggcactatg attttgcaa acgggtgac 
      gaaatcaac gaaatcgat aatacgggc tttgccact 
      cacatcccg accgtttac gctggtaat ttaggaaac 
      ttgaagtac attgtgcag gttacaagc agcgctgtg 
      cgactttta caagggata aaccaattg caacacatt 
      cccctggaa ttggggtcc cccatagtc cacgtgaca 
      agtgtcgac ccctacatt tccctacta acaacagac 
      ggtcaagtg atcacactc atgctccgg gaagcccga 
      ggggtcgct aaactagtc ataagcaaa tcaactctg 
      tcaaactcg ccccccgtc accaccata tgcatgtac 
      cgcgacgtt tcgggcctt ttcacgtcg aaaatcccc 
      gaggatttc acgcacatc cccgagcat ttcataaac 
      gaatccgag acgaaaatg gaagtggaa aacgaggac 
      gatctgctc tatggagac gacagcgat tttaaaatg 
      ccaactctg aacccacca cagcccaaa ccgaaagtt 
      tactacaac tggtggaaa aaatatttg ttggacgtc 
      cggccctcg tattggctt ttcgtcgta cgcgaaaat 
      tccaatttg gagatttac tcaatcccc gattttaaa 
      ttgtgttat tacatcacg aatttgtgc ttcggtcat 
      aaagtcctg gtcgataat ttggaatcg gtgacgatt 
      tcagcctcg acacccatt tccgcggca catgaagcc 
      aatatccag aggcagttc gatgttaag gaaattctc 
      gtcgttgcg ttgggaaac cacggctca agaccactt 
      ttaatggtc agattggag cgcgatttg tacatttat 
      gaagtgttt aggtttccg agagggaat ttgaaaatg 
      cgcttcagg aaaatcaaa cattcgctg atttattcg 
      ccaaatgtg tctgggaga attgacacg gaagattcg 
      gattttttc gcaatacag gagcgcatt attaaaatg 
      cgatacttt accaatatt gcagggtat aatggcgtt 
      ttcgtgtgt ggggccaac ccacattgg atttttatg 
      tcagccagg ggtgagcta aggacacat ccgatgaca 
      attgacggc gaagtgctc agttttgcg gcttttaat 
      aatgtgaac tgtcctcaa ggttttctt tatttcaac 
      aggaagtct gagttgaga attggagtt ctcccaacc 
      cacctaagc tacgacgcc gcctggccg gtccgcaag 
      gtccccctc aggtgcacc ccacacttc gtaacatac 
      catttagaa tcaaaaacc tactgcttg gttacaagc 
      attgccgaa ccgtcgaac aaatattac aaattcaac 
      ggcgaagac aaagaattg tcagttgaa gacagaggc 
      gaccgattt ccatacccc cttcaggaa aaattcagt 
      ctcatgcta ttttcgccc gtatcttgg gacgtaatt 
      cccaataca aaaattgat ttagacgag tgggagcac 
      gtgaattgt ttgaaaaac gtttctttg gcttatgaa 
      ggcacaagg tcgggtttg aaaggctac attgcagtg 
      gggaccaat tataattac ggagaagat gtgacgtct 
      cgtgggcgg attctgatt tttgatatc attgaggtg 
      gtcccagaa ccgggccaa ccactgacc aaaaacagg 
      ttcaaggaa atatatgcc aaggaccag aaagggcct 
      gttacggcc ctaagtcaa gtcaaaggt tttctagtc 
      tcagcagtg gggcaaaaa atttatatt tggcagctg 
      aaagacaat gatttggtc ggtgtcgcg ttcatagac 
      acgcaaata tacacgcac cagattcta acaattaag 
      agtttactg cttgtggca gatgtttac aagtccata 
      tcactcctc cgttttcaa gaggaatac cggacgttg 
      tcacttgtt tcaagggat tttcggccg tgtgaggta 
      ttttcggtt gagtacatg atcgataac acaacgatg 
      ggctttctt gtctcagac agtgagaaa aatttggtt 
      ttgtacatg tatcagccc gaatcgagg gagtcttta 
      ggggggcaa aggctacta cgcaaagct gattttcac 
      ctgggacaa gctgttaac tcgttcttt aggattaag 
      tgcaaactt ggtgagttg ggagaagac aagaaaaat 
      ttaacgggc gctgacaag cgccacatc actatgtat 
      gcaactttg gatggaggt cttggctac ataatgccg 
      gttcctgaa aaaacttac cgcagactg ttgatgttg 
      caaaatgtg ctggtctcc caaggagca cacattgct 
      ggattaaat cccaaagca tttaggacg tacaaaagc 
      tggaagaag cttcagacc aatccggcc cgatctgta 
      attgacggt gaactcgtc tacaactat ctacagtta 
      tcaattcca gaaaaatta gaagtttcg aaaaagata 
      ggaactaaa ctagaggag ttacttgat gatctatcg 
      gacattcag aaaattaca aatcatttt taa
    mRNA sequence
    • >TC003068 RA
      gttatttta tgtttttgc atttttgta atcttaatc 
      caaaatgtt atggttcaa ataaagttc acaattcaa 
      aataaactg aatgaactc cagccccca aatatgcta 
      aaccgacaa aactattca taattccgc aacttaaac 
      aatttcccg tgtgttctt gttgcaaat taaattatt 
      ccaatgttt tctatttgt aaacaaacg caccctgcc 
      acgggggtg gaacacgca atttcgtgc cattttttc 
      agcaagtct gagaaaagt ttagttacg tcaggggcc 
      aacgtaatc aaagtgttt cgcctgatc ccagacatt 
      gacacaaaa accagaatt gataaattt aatgaaaca 
      aaccctccg aagtctaag ctagaatgt gtggctcaa 
      tacactttg tttggaaat atcatgtcc atgcaaagc 
      gtaaatctt gcaaattcg cccagagac gctctgtta 
      ttagcgttc aaagacgca aaattatca gtggtggaa 
      tatgacccc gaaacgcac gatttgaag actctgagc 
      ttgcactac tttgaggaa gatgacatg aaggacggt 
      tggacccac cattaccat gtgcctatg gtgcgagct 
      gatcctgaa aaccgctgc gctgttatg accgttttc 
      ggccgaaaa ttggtagtt cttcctttc aggcgtgaa 
      aacgcaatt gatgacacc gatgctgat attaagcca 
      atgattggt ggagcttac ggatcaaaa gcgccaatc 
      ttggcctct tatatgatt gttttaaag gactttatc 
      gataaagtt gacaacata atagacatc cagttcttg 
      cacggttat tacgagcca acgctcctg attttgttc 
      gagcctttg aaaactttt gctgggcgc gtcgctgtg 
      cgaactgat acgtgtgcc atggctgca atttccctc 
      aacttgcag caaaaagtg cacccgatt atttggagt 
      gttgcaaat ttgccgttt gattgtgta aaagccgtt 
      cctattaaa aaaccgctc ggtggcacg ttgattttt 
      gcagtaaat gcactaatt tatctaaat caaagtatt 
      ccaccgtat ggggtttcc ttgaacagt attgccgaa 
      aattcaaca aattttcca ttaaaaccg caggacgat 
      ttatgtata agtttagat tgtgcacaa gccacgttt 
      ttggaagac gatacaatt gtcttgtct ctgaaaggg 
      ggcgaactt tacgtccta actttgctg gcagacaat 
      atgcgatat gttaggagt tttcatttt gagaaagct 
      gcagccagt gttctcaca acatgtatc tctgtatgt 
      gagaacaac tttttgttc ctcggctcg cgcctcgga 
      aactccctt cttttgcgc ttcactgaa aaatgcaac 
      gaagtcata actctggat gaaacgatc gaaccgagc 
      gccaaacgc ctgaaagct agcaatagt acaagtgaa 
      aatgaggac gataaagtc ctggacacg ttaaacgat 
      tgtatggct agtgatgtg ttagatatt cgcgatccg 
      gaagaactg gaagtgtac ggaaatcaa aagcaagcc 
      agtcttcaa atatcaagc tacgtcttt gaagtctgt 
      gatagttta ctcaacatt ggcccttgt ggcaatatc 
      tcgctagga gaaccggct tttctgagc gaggaattt 
      tccgaaaac cttgattta gatttggaa cttgttaca 
      acagcaggg tatgggaaa aacggggcg ctttgtgtg 
      ttacagaag tctgtaagg ccgcaaatt gtgaccact 
      tttacgcta cctgggtgc tccaatatg tggactgtg 
      catgccggg gaggacaaa catgcgttt ctcattttg 
      agtcaagag gacggcact atgattttg caaacgggt 
      gacgaaatc aacgaaatc gataatacg ggctttgcc 
      actcacatc ccgaccgtt tacgctggt aatttagga 
      aacttgaag tacattgtg caggttaca agcagcgct 
      gtgcgactt ttacaaggg ataaaccaa ttgcaacac 
      attcccctg gaattgggg tcccccata gtccacgtg 
      acaagtgtc gacccctac atttcccta ctaacaaca 
      gacggtcaa gtgatcaca ctcatgctc cgggaagcc 
      cgaggggtc gctaaacta gtcataagc aaatcaact 
      ctgtcaaac tcgcccccc gtcaccacc atatgcatg 
      taccgcgac gtttcgggc cttttcacg tcgaaaatc 
      cccgaggat ttcacgcac atccccgag catttcata 
      aacgaatcc gagacgaaa atggaagtg gaaaacgag 
      gacgatctg ctctatgga gacgacagc gattttaaa 
      atgccaact ctgaaccca ccacagccc aaaccgaaa 
      gtttactac aactggtgg aaaaaatat ttgttggac 
      gtccggccc tcgtattgg cttttcgtc gtacgcgaa 
      aattccaat ttggagatt tactcaatc cccgatttt 
      aaattgtgt tattacatc acgaatttg tgcttcggt 
      cataaagtc ctggtcgat aatttggaa tcggtgacg 
      atttcagcc tcgacaccc atttccgcg gcacatgaa 
      gccaatatc cagaggcag ttcgatgtt aaggaaatt 
      ctcgtcgtt gcgttggga aaccacggc tcaagacca 
      cttttaatg gtcagattg gagcgcgat ttgtacatt 
      tatgaagtg tttaggttt ccgagaggg aatttgaaa 
      atgcgcttc aggaaaatc aaacattcg ctgatttat 
      tcgccaaat gtgtctggg agaattgac acggaagat 
      tcggatttt ttcgcaata caggagcgc attattaaa 
      atgcgatac tttaccaat attgcaggg tataatggc 
      gttttcgtg tgtggggcc aacccacat tggattttt 
      atgtcagcc aggggtgag ctaaggaca catccgatg 
      acaattgac ggcgaagtg ctcagtttt gcggctttt 
      aataatgtg aactgtcct caaggtttt ctttatttc 
      aacaggaag tctgagttg agaattgga gttctccca 
      acccaccta agctacgac gccgcctgg ccggtccgc 
      aaggtcccc ctcaggtgc accccacac ttcgtaaca 
      taccattta gaatcaaaa acctactgc ttggttaca 
      agcattgcc gaaccgtcg aacaaatat tacaaattc 
      aacggcgaa gacaaagaa ttgtcagtt gaagacaga 
      ggcgaccga tttccatac ccccttcag gaaaaattc 
      agtctcatg ctattttcg cccgtatct tgggacgta 
      attcccaat acaaaaatt gatttagac gagtgggag 
      cacgtgaat tgtttgaaa aacgtttct ttggcttat 
      gaaggcaca aggtcgggt ttgaaaggc tacattgca 
      gtggggacc aattataat tacggagaa gatgtgacg 
      tctcgtggg cggattctg atttttgat atcattgag 
      gtggtccca gaaccgggc caaccactg accaaaaac 
      aggttcaag gaaatatat gccaaggac cagaaaggg 
      cctgttacg gccctaagt caagtcaaa ggttttcta 
      gtctcagca gtggggcaa aaaatttat atttggcag 
      ctgaaagac aatgatttg gtcggtgtc gcgttcata 
      gacacgcaa atatacacg caccagatt ctaacaatt 
      aagagttta ctgcttgtg gcagatgtt tacaagtcc 
      atatcactc ctccgtttt caagaggaa taccggacg 
      ttgtcactt gtttcaagg gattttcgg ccgtgtgag 
      gtattttcg gttgagtac atgatcgat aacacaacg 
      atgggcttt cttgtctca gacagtgag aaaaatttg 
      gttttgtac atgtatcag cccgaatcg agggagtct 
      ttagggggg caaaggcta ctacgcaaa gctgatttt 
      cacctggga caagctgtt aactcgttc tttaggatt 
      aagtgcaaa cttggtgag ttgggagaa gacaagaaa 
      aatttaacg ggcgctgac aagcgccac atcactatg 
      tatgcaact ttggatgga ggtcttggc tacataatg 
      ccggttcct gaaaaaact taccgcaga ctgttgatg 
      ttgcaaaat gtgctggtc tcccaagga gcacacatt 
      gctggatta aatcccaaa gcatttagg acgtacaaa 
      agctggaag aagcttcag accaatccg gcccgatct 
      gtaattgac ggtgaactc gtctacaac tatctacag 
      ttatcaatt ccagaaaaa ttagaagtt tcgaaaaag 
      ataggaact aaactagag gagttactt gatgatcta 
      tcggacatt cagaaaatt acaaatcat ttttaagtt 
      cgtttttta ccaaaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY