| General information | |||
| TC003068 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 2801239..2809426 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003068 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03491 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC003068 RA
MFSICKQTH PATGVEHAI SCHFFSKSE KSLVTSGAN VIKVFRLIP DIDTKTRID KFNETNPPK SKLECVAQY TLFGNIMSM QSVNLANSP RDALLLAFK DAKLSVVEY DPETHDLKT LSLHYFEED DMKDGWTHH YHVPMVRAD PENRCAVMT VFGRKLVVL PFRRENAID DTDADIKPM IGGAYGSKA PILASYMIV LKDFIDKVD NIIDIQFLH GYYEPTLLI LFEPLKTFA GRVAVRTDT CAMAAISLN LQQKVHPII WSVANLPFD CVKAVPIKK PLGGTLIFA VNALIYLNQ SIPPYGVSL NSIAENSTN FPLKPQDDL CISLDCAQA TFLEDDTIV LSLKGGELY VLTLLADNM RYVRSFHFE KAAASVLTT CISVCENNF LFLGSRLGN SLLLRFTEK CNEVITLDE TIEPSAKRL KASNSTSEN EDDKVLDTL NDCMASDVL DIRDPEELE VYGNQKQAS LQISSYVFE VCDSLLNIG PCGNISLGE PAFLSEEFS ENLDLDLEL VTTAGYGKN GALCVLQKS VRPQIVTTF TLPGCSNMW TVHAGEDKH AFLILSQED GTMILQTGD EINEIDNTG FATHIPTVY AGNLGNLKY IVQVTSSAV RLLQGINQL QHIPLELGS PIVHVTSVD PYISLLTTD GQVITLMLR EARGVAKLV ISKSTLSNS PPVTTICMY RDVSGLFTS KIPEDFTHI PEHFINESE TKMEVENED DLLYGDDSD FKMPTLNPP QPKPKVYYN WWKKYLLDV RPSYWLFVV RENSNLEIY SIPDFKLCY YITNLCFGH KVLVDNLES VTISASTPI SAAHEANIQ RQFDVKEIL VVALGNHGS RPLLMVRLE RDLYIYEVF RFPRGNLKM RFRKIKHSL IYSPNVSGR IDTEDSDFF AIQERIIKM RYFTNIAGY NGVFVCGAN PHWIFMSAR GELRTHPMT IDGEVLSFA AFNNVNCPQ GFLYFNRKS ELRIGVLPT HLSYDAAWP VRKVPLRCT PHFVTYHLE SKTYCLVTS IAEPSNKYY KFNGEDKEL SVEDRGDRF PYPLQEKFS LMLFSPVSW DVIPNTKID LDEWEHVNC LKNVSLAYE GTRSGLKGY IAVGTNYNY GEDVTSRGR ILIFDIIEV VPEPGQPLT KNRFKEIYA KDQKGPVTA LSQVKGFLV SAVGQKIYI WQLKDNDLV GVAFIDTQI YTHQILTIK SLLLVADVY KSISLLRFQ EEYRTLSLV SRDFRPCEV FSVEYMIDN TTMGFLVSD SEKNLVLYM YQPESRESL GGQRLLRKA DFHLGQAVN SFFRIKCKL GELGEDKKN LTGADKRHI TMYATLDGG LGYIMPVPE KTYRRLLML QNVLVSQGA HIAGLNPKA FRTYKSWKK LQTNPARSV IDGELVYNY LQLSIPEKL EVSKKIGTK LEELLDDLS DIQKITNHF
- >TC003068 RA
atgttttct atttgtaaa caaacgcac cctgccacg ggggtggaa cacgcaatt tcgtgccat tttttcagc aagtctgag aaaagttta gttacgtca ggggccaac gtaatcaaa gtgtttcgc ctgatccca gacattgac acaaaaacc agaattgat aaatttaat gaaacaaac cctccgaag tctaagcta gaatgtgtg gctcaatac actttgttt ggaaatatc atgtccatg caaagcgta aatcttgca aattcgccc agagacgct ctgttatta gcgttcaaa gacgcaaaa ttatcagtg gtggaatat gaccccgaa acgcacgat ttgaagact ctgagcttg cactacttt gaggaagat gacatgaag gacggttgg acccaccat taccatgtg cctatggtg cgagctgat cctgaaaac cgctgcgct gttatgacc gttttcggc cgaaaattg gtagttctt cctttcagg cgtgaaaac gcaattgat gacaccgat gctgatatt aagccaatg attggtgga gcttacgga tcaaaagcg ccaatcttg gcctcttat atgattgtt ttaaaggac tttatcgat aaagttgac aacataata gacatccag ttcttgcac ggttattac gagccaacg ctcctgatt ttgttcgag cctttgaaa acttttgct gggcgcgtc gctgtgcga actgatacg tgtgccatg gctgcaatt tccctcaac ttgcagcaa aaagtgcac ccgattatt tggagtgtt gcaaatttg ccgtttgat tgtgtaaaa gccgttcct attaaaaaa ccgctcggt ggcacgttg atttttgca gtaaatgca ctaatttat ctaaatcaa agtattcca ccgtatggg gtttccttg aacagtatt gccgaaaat tcaacaaat tttccatta aaaccgcag gacgattta tgtataagt ttagattgt gcacaagcc acgtttttg gaagacgat acaattgtc ttgtctctg aaagggggc gaactttac gtcctaact ttgctggca gacaatatg cgatatgtt aggagtttt cattttgag aaagctgca gccagtgtt ctcacaaca tgtatctct gtatgtgag aacaacttt ttgttcctc ggctcgcgc ctcggaaac tcccttctt ttgcgcttc actgaaaaa tgcaacgaa gtcataact ctggatgaa acgatcgaa ccgagcgcc aaacgcctg aaagctagc aatagtaca agtgaaaat gaggacgat aaagtcctg gacacgtta aacgattgt atggctagt gatgtgtta gatattcgc gatccggaa gaactggaa gtgtacgga aatcaaaag caagccagt cttcaaata tcaagctac gtctttgaa gtctgtgat agtttactc aacattggc ccttgtggc aatatctcg ctaggagaa ccggctttt ctgagcgag gaattttcc gaaaacctt gatttagat ttggaactt gttacaaca gcagggtat gggaaaaac ggggcgctt tgtgtgtta cagaagtct gtaaggccg caaattgtg accactttt acgctacct gggtgctcc aatatgtgg actgtgcat gccggggag gacaaacat gcgtttctc attttgagt caagaggac ggcactatg attttgcaa acgggtgac gaaatcaac gaaatcgat aatacgggc tttgccact cacatcccg accgtttac gctggtaat ttaggaaac ttgaagtac attgtgcag gttacaagc agcgctgtg cgactttta caagggata aaccaattg caacacatt cccctggaa ttggggtcc cccatagtc cacgtgaca agtgtcgac ccctacatt tccctacta acaacagac ggtcaagtg atcacactc atgctccgg gaagcccga ggggtcgct aaactagtc ataagcaaa tcaactctg tcaaactcg ccccccgtc accaccata tgcatgtac cgcgacgtt tcgggcctt ttcacgtcg aaaatcccc gaggatttc acgcacatc cccgagcat ttcataaac gaatccgag acgaaaatg gaagtggaa aacgaggac gatctgctc tatggagac gacagcgat tttaaaatg ccaactctg aacccacca cagcccaaa ccgaaagtt tactacaac tggtggaaa aaatatttg ttggacgtc cggccctcg tattggctt ttcgtcgta cgcgaaaat tccaatttg gagatttac tcaatcccc gattttaaa ttgtgttat tacatcacg aatttgtgc ttcggtcat aaagtcctg gtcgataat ttggaatcg gtgacgatt tcagcctcg acacccatt tccgcggca catgaagcc aatatccag aggcagttc gatgttaag gaaattctc gtcgttgcg ttgggaaac cacggctca agaccactt ttaatggtc agattggag cgcgatttg tacatttat gaagtgttt aggtttccg agagggaat ttgaaaatg cgcttcagg aaaatcaaa cattcgctg atttattcg ccaaatgtg tctgggaga attgacacg gaagattcg gattttttc gcaatacag gagcgcatt attaaaatg cgatacttt accaatatt gcagggtat aatggcgtt ttcgtgtgt ggggccaac ccacattgg atttttatg tcagccagg ggtgagcta aggacacat ccgatgaca attgacggc gaagtgctc agttttgcg gcttttaat aatgtgaac tgtcctcaa ggttttctt tatttcaac aggaagtct gagttgaga attggagtt ctcccaacc cacctaagc tacgacgcc gcctggccg gtccgcaag gtccccctc aggtgcacc ccacacttc gtaacatac catttagaa tcaaaaacc tactgcttg gttacaagc attgccgaa ccgtcgaac aaatattac aaattcaac ggcgaagac aaagaattg tcagttgaa gacagaggc gaccgattt ccatacccc cttcaggaa aaattcagt ctcatgcta ttttcgccc gtatcttgg gacgtaatt cccaataca aaaattgat ttagacgag tgggagcac gtgaattgt ttgaaaaac gtttctttg gcttatgaa ggcacaagg tcgggtttg aaaggctac attgcagtg gggaccaat tataattac ggagaagat gtgacgtct cgtgggcgg attctgatt tttgatatc attgaggtg gtcccagaa ccgggccaa ccactgacc aaaaacagg ttcaaggaa atatatgcc aaggaccag aaagggcct gttacggcc ctaagtcaa gtcaaaggt tttctagtc tcagcagtg gggcaaaaa atttatatt tggcagctg aaagacaat gatttggtc ggtgtcgcg ttcatagac acgcaaata tacacgcac cagattcta acaattaag agtttactg cttgtggca gatgtttac aagtccata tcactcctc cgttttcaa gaggaatac cggacgttg tcacttgtt tcaagggat tttcggccg tgtgaggta ttttcggtt gagtacatg atcgataac acaacgatg ggctttctt gtctcagac agtgagaaa aatttggtt ttgtacatg tatcagccc gaatcgagg gagtcttta ggggggcaa aggctacta cgcaaagct gattttcac ctgggacaa gctgttaac tcgttcttt aggattaag tgcaaactt ggtgagttg ggagaagac aagaaaaat ttaacgggc gctgacaag cgccacatc actatgtat gcaactttg gatggaggt cttggctac ataatgccg gttcctgaa aaaacttac cgcagactg ttgatgttg caaaatgtg ctggtctcc caaggagca cacattgct ggattaaat cccaaagca tttaggacg tacaaaagc tggaagaag cttcagacc aatccggcc cgatctgta attgacggt gaactcgtc tacaactat ctacagtta tcaattcca gaaaaatta gaagtttcg aaaaagata ggaactaaa ctagaggag ttacttgat gatctatcg gacattcag aaaattaca aatcatttt taa
- >TC003068 RA
gttatttta tgtttttgc atttttgta atcttaatc caaaatgtt atggttcaa ataaagttc acaattcaa aataaactg aatgaactc cagccccca aatatgcta aaccgacaa aactattca taattccgc aacttaaac aatttcccg tgtgttctt gttgcaaat taaattatt ccaatgttt tctatttgt aaacaaacg caccctgcc acgggggtg gaacacgca atttcgtgc cattttttc agcaagtct gagaaaagt ttagttacg tcaggggcc aacgtaatc aaagtgttt cgcctgatc ccagacatt gacacaaaa accagaatt gataaattt aatgaaaca aaccctccg aagtctaag ctagaatgt gtggctcaa tacactttg tttggaaat atcatgtcc atgcaaagc gtaaatctt gcaaattcg cccagagac gctctgtta ttagcgttc aaagacgca aaattatca gtggtggaa tatgacccc gaaacgcac gatttgaag actctgagc ttgcactac tttgaggaa gatgacatg aaggacggt tggacccac cattaccat gtgcctatg gtgcgagct gatcctgaa aaccgctgc gctgttatg accgttttc ggccgaaaa ttggtagtt cttcctttc aggcgtgaa aacgcaatt gatgacacc gatgctgat attaagcca atgattggt ggagcttac ggatcaaaa gcgccaatc ttggcctct tatatgatt gttttaaag gactttatc gataaagtt gacaacata atagacatc cagttcttg cacggttat tacgagcca acgctcctg attttgttc gagcctttg aaaactttt gctgggcgc gtcgctgtg cgaactgat acgtgtgcc atggctgca atttccctc aacttgcag caaaaagtg cacccgatt atttggagt gttgcaaat ttgccgttt gattgtgta aaagccgtt cctattaaa aaaccgctc ggtggcacg ttgattttt gcagtaaat gcactaatt tatctaaat caaagtatt ccaccgtat ggggtttcc ttgaacagt attgccgaa aattcaaca aattttcca ttaaaaccg caggacgat ttatgtata agtttagat tgtgcacaa gccacgttt ttggaagac gatacaatt gtcttgtct ctgaaaggg ggcgaactt tacgtccta actttgctg gcagacaat atgcgatat gttaggagt tttcatttt gagaaagct gcagccagt gttctcaca acatgtatc tctgtatgt gagaacaac tttttgttc ctcggctcg cgcctcgga aactccctt cttttgcgc ttcactgaa aaatgcaac gaagtcata actctggat gaaacgatc gaaccgagc gccaaacgc ctgaaagct agcaatagt acaagtgaa aatgaggac gataaagtc ctggacacg ttaaacgat tgtatggct agtgatgtg ttagatatt cgcgatccg gaagaactg gaagtgtac ggaaatcaa aagcaagcc agtcttcaa atatcaagc tacgtcttt gaagtctgt gatagttta ctcaacatt ggcccttgt ggcaatatc tcgctagga gaaccggct tttctgagc gaggaattt tccgaaaac cttgattta gatttggaa cttgttaca acagcaggg tatgggaaa aacggggcg ctttgtgtg ttacagaag tctgtaagg ccgcaaatt gtgaccact tttacgcta cctgggtgc tccaatatg tggactgtg catgccggg gaggacaaa catgcgttt ctcattttg agtcaagag gacggcact atgattttg caaacgggt gacgaaatc aacgaaatc gataatacg ggctttgcc actcacatc ccgaccgtt tacgctggt aatttagga aacttgaag tacattgtg caggttaca agcagcgct gtgcgactt ttacaaggg ataaaccaa ttgcaacac attcccctg gaattgggg tcccccata gtccacgtg acaagtgtc gacccctac atttcccta ctaacaaca gacggtcaa gtgatcaca ctcatgctc cgggaagcc cgaggggtc gctaaacta gtcataagc aaatcaact ctgtcaaac tcgcccccc gtcaccacc atatgcatg taccgcgac gtttcgggc cttttcacg tcgaaaatc cccgaggat ttcacgcac atccccgag catttcata aacgaatcc gagacgaaa atggaagtg gaaaacgag gacgatctg ctctatgga gacgacagc gattttaaa atgccaact ctgaaccca ccacagccc aaaccgaaa gtttactac aactggtgg aaaaaatat ttgttggac gtccggccc tcgtattgg cttttcgtc gtacgcgaa aattccaat ttggagatt tactcaatc cccgatttt aaattgtgt tattacatc acgaatttg tgcttcggt cataaagtc ctggtcgat aatttggaa tcggtgacg atttcagcc tcgacaccc atttccgcg gcacatgaa gccaatatc cagaggcag ttcgatgtt aaggaaatt ctcgtcgtt gcgttggga aaccacggc tcaagacca cttttaatg gtcagattg gagcgcgat ttgtacatt tatgaagtg tttaggttt ccgagaggg aatttgaaa atgcgcttc aggaaaatc aaacattcg ctgatttat tcgccaaat gtgtctggg agaattgac acggaagat tcggatttt ttcgcaata caggagcgc attattaaa atgcgatac tttaccaat attgcaggg tataatggc gttttcgtg tgtggggcc aacccacat tggattttt atgtcagcc aggggtgag ctaaggaca catccgatg acaattgac ggcgaagtg ctcagtttt gcggctttt aataatgtg aactgtcct caaggtttt ctttatttc aacaggaag tctgagttg agaattgga gttctccca acccaccta agctacgac gccgcctgg ccggtccgc aaggtcccc ctcaggtgc accccacac ttcgtaaca taccattta gaatcaaaa acctactgc ttggttaca agcattgcc gaaccgtcg aacaaatat tacaaattc aacggcgaa gacaaagaa ttgtcagtt gaagacaga ggcgaccga tttccatac ccccttcag gaaaaattc agtctcatg ctattttcg cccgtatct tgggacgta attcccaat acaaaaatt gatttagac gagtgggag cacgtgaat tgtttgaaa aacgtttct ttggcttat gaaggcaca aggtcgggt ttgaaaggc tacattgca gtggggacc aattataat tacggagaa gatgtgacg tctcgtggg cggattctg atttttgat atcattgag gtggtccca gaaccgggc caaccactg accaaaaac aggttcaag gaaatatat gccaaggac cagaaaggg cctgttacg gccctaagt caagtcaaa ggttttcta gtctcagca gtggggcaa aaaatttat atttggcag ctgaaagac aatgatttg gtcggtgtc gcgttcata gacacgcaa atatacacg caccagatt ctaacaatt aagagttta ctgcttgtg gcagatgtt tacaagtcc atatcactc ctccgtttt caagaggaa taccggacg ttgtcactt gtttcaagg gattttcgg ccgtgtgag gtattttcg gttgagtac atgatcgat aacacaacg atgggcttt cttgtctca gacagtgag aaaaatttg gttttgtac atgtatcag cccgaatcg agggagtct ttagggggg caaaggcta ctacgcaaa gctgatttt cacctggga caagctgtt aactcgttc tttaggatt aagtgcaaa cttggtgag ttgggagaa gacaagaaa aatttaacg ggcgctgac aagcgccac atcactatg tatgcaact ttggatgga ggtcttggc tacataatg ccggttcct gaaaaaact taccgcaga ctgttgatg ttgcaaaat gtgctggtc tcccaagga gcacacatt gctggatta aatcccaaa gcatttagg acgtacaaa agctggaag aagcttcag accaatccg gcccgatct gtaattgac ggtgaactc gtctacaac tatctacag ttatcaatt ccagaaaaa ttagaagtt tcgaaaaag ataggaact aaactagag gagttactt gatgatcta tcggacatt cagaaaatt acaaatcat ttttaagtt cgtttttta ccaaaata
