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Gene information
General information
TC003034 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4044182..4048845
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003034
    iBeetle screen iB_03489

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003034 RA
      MSKFKMFSD DYEQGYEYN SYDEYGAHT GDPNLDNEY 
      NKQHLKMPE IVKNFLIYF RNCVNEGLI FELQTLYET 
      SWPKLTEDY FEKRPWPEE SEVSALVDS DPVFMILYK 
      ELYFRHIYA RIQGGPTLE QRFNSFYNY CDLFNYILS 
      ADEPVPLEL PDLWLWELI DEFVYQFQS FAQYRARLQ 
      KKTAQELET LNSHNKVWN VLCVLNVLH SLVDKSNIK 
      QQLEVCASG GDPDSVAGE FGSHSLYKM LGYFSLIGL 
      LRLHSLLGD YYQSIKVLE NIELHKKSQ YAHIPACLI 
      STSYYVGFG YMMMRRYSD AIRTFSSIL LYIQRTKQL 
      FASKSYQHD QINKQTDQM YHLLAICLV LHPQCIDES 
      IQQTLREKS YHEKMYKMQ YGDLQEFEN CFVFACPKF 
      LSPCPPPVD SIPDDYSKE AIRHQTQVF MDEVQQQKM 
      LPTIRSYLK LYTTLPIAK LATFMAQNK NSEGKFDVD 
      KETQNLLIH LLCFKHKMK NVVWSKGSS GLEGKLESG 
      SELDFYIDN DMIHIADTK VAHRYGDFF IRKILKFED 
      LNRKLHNLK F
    CDS sequence
    • >TC003034 RA
      atgagtaaa ttcaaaatg ttttccgac gattatgag 
      caaggttat gaatacaac agctatgat gagtatggg 
      gctcacaca ggagacccc aacttggac aacgagtac 
      aataaacag cacctaaag atgccggaa atagtcaaa 
      aacttccta atctatttc cgcaattgc gtgaatgag 
      ggtttaatc tttgagttg caaactttg tatgaaact 
      tcgtggcca aaactaacc gaagattat ttcgagaaa 
      cgcccctgg ccagaagag tcagaagtg tcagcgcta 
      gtcgacagt gatcccgtg ttcatgatc ctgtacaaa 
      gagctctac ttccgccac atttacgcg cgcattcaa 
      gggggccca accctcgag cagcgcttc aattcgttc 
      tacaattac tgcgacctc ttcaactac atcctaagc 
      gctgacgaa ccggtgcct ctagaactc cccgacttg 
      tggctatgg gaattaatc gacgaattc gtctaccaa 
      ttccaatca ttcgcccaa taccgggcc cgccttcag 
      aagaaaacc gcccaagaa ctggagact ctaaactcg 
      cacaataaa gtgtggaac gtactttgc gttctgaac 
      gtgttgcat tctctggtc gacaagagc aacatcaag 
      caacaactg gaggtttgc gccagtggg ggcgacccc 
      gattcggtg gccggcgag ttcgggagt cactcgctc 
      tacaaaatg ttgggctat ttttccctg attgggctt 
      ctgaggctt cactctctg ctcggggac tactaccag 
      tcgatcaaa gtgctggaa aacattgag ctgcataag 
      aagtcgcag tatgcgcac atcccggct tgcttgatc 
      tcaacgtcg tactatgtg gggtttggg tacatgatg 
      atgaggcgc tattctgac gcaattcgc acgttttcg 
      tccattctc ttgtacatt cagagaacc aagcaattg 
      tttgcttct aaaagttat cagcacgac cagattaac 
      aagcaaacg gatcagatg taccacttg ttggccatt 
      tgcttggtt ttgcacccg cagtgtatc gacgaatcg 
      atccagcaa actctgaga gagaaaagc taccatgag 
      aaaatgtac aagatgcag tatggggac ctccaggag 
      tttgaaaat tgtttcgtt ttcgcttgt cctaagttt 
      ctttcgccg tgtcctccg ccggtggat tccatccct 
      gatgattat tcgaaggag gcgatccgc caccaaact 
      caagttttt atggacgag gtccagcaa caaaaaatg 
      ttgccaacg attagaagt tacttgaag ttgtacacc 
      actttgccc attgccaag ttggcgact ttcatggcg 
      cagaataag aacagtgaa gggaagttt gatgttgat 
      aaggagacg cagaatctt ttgattcat ttgttgtgc 
      ttcaagcac aaaatgaag aatgtggtg tggtcgaag 
      ggctcgtcg gggctggaa gggaagctg gagagtggc 
      tcagagctg gatttctac atcgataat gatatgatt 
      catatcgcc gacacgaaa gtggcgcac agatacggc 
      gatttcttt ataaggaaa attctcaag tttgaggat 
      ttgaacagg aagcttcac aatcttaaa ttctaa
    mRNA sequence
    • >TC003034 RA
      catcatgtt tattgatcg ttttatttt aaaacattt 
      ttctcactt actcagcgg aaatacttt tgaaaatga 
      gaaaaaaat ggaatttct tgcaccacc tcgagtaga 
      aacctgtaa tttgtaatt gtcaaacgt caactggct 
      tcgaggtta agtgcaaga ccatttgct cgctatagt 
      ggcgtaggc atataatga gtaaattca aaatgtttt 
      ccgacgatt atgagcaag gttatgaat acaacagct 
      atgatgagt atggggctc acacaggag accccaact 
      tggacaacg agtacaata aacagcacc taaagatgc 
      cggaaatag tcaaaaact tcctaatct atttccgca 
      attgcgtga atgagggtt taatctttg agttgcaaa 
      ctttgtatg aaacttcgt ggccaaaac taaccgaag 
      attatttcg agaaacgcc cctggccag aagagtcag 
      aagtgtcag cgctagtcg acagtgatc ccgtgttca 
      tgatcctgt acaaagagc tctacttcc gccacattt 
      acgcgcgca ttcaagggg gcccaaccc tcgagcagc 
      gcttcaatt cgttctaca attactgcg acctcttca 
      actacatcc taagcgctg acgaaccgg tgcctctag 
      aactccccg acttgtggc tatgggaat taatcgacg 
      aattcgtct accaattcc aatcattcg cccaatacc 
      gggcccgcc ttcagaaga aaaccgccc aagaactgg 
      agactctaa actcgcaca ataaagtgt ggaacgtac 
      tttgcgttc tgaacgtgt tgcattctc tggtcgaca 
      agagcaaca tcaagcaac aactggagg tttgcgcca 
      gtgggggcg accccgatt cggtggccg gcgagttcg 
      ggagtcact cgctctaca aaatgttgg gctattttt 
      ccctgattg ggcttctga ggcttcact ctctgctcg 
      gggactact accagtcga tcaaagtgc tggaaaaca 
      ttgagctgc ataagaagt cgcagtatg cgcacatcc 
      cggcttgct tgatctcaa cgtcgtact atgtggggt 
      ttgggtaca tgatgatga ggcgctatt ctgacgcaa 
      ttcgcacgt tttcgtcca ttctcttgt acattcaga 
      gaaccaagc aattgtttg cttctaaaa gttatcagc 
      acgaccaga ttaacaagc aaacggatc agatgtacc 
      acttgttgg ccatttgct tggttttgc acccgcagt 
      gtatcgacg aatcgatcc agcaaactc tgagagaga 
      aaagctacc atgagaaaa tgtacaaga tgcagtatg 
      gggacctcc aggagtttg aaaattgtt tcgttttcg 
      cttgtccta agtttcttt cgccgtgtc ctccgccgg 
      tggattcca tccctgatg attattcga aggaggcga 
      tccgccacc aaactcaag tttttatgg acgaggtcc 
      agcaacaaa aaatgttgc caacgatta gaagttact 
      tgaagttgt acaccactt tgcccattg ccaagttgg 
      cgactttca tggcgcaga ataagaaca gtgaaggga 
      agtttgatg ttgataagg agacgcaga atcttttga 
      ttcatttgt tgtgcttca agcacaaaa tgaagaatg 
      tggtgtggt cgaagggct cgtcggggc tggaaggga 
      agctggaga gtggctcag agctggatt tctacatcg 
      ataatgata tgattcata tcgccgaca cgaaagtgg 
      cgcacagat acggcgatt tctttataa ggaaaattc 
      tcaagtttg aggatttga acaggaagc ttcacaatc 
      ttaaattct aatgtagaa ttcggtgta atttttgca 
      ctcaacgaa taaaattgc ttaaaataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY