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Gene information
General information
TC003033 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4032302..4034249
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003033
    iBeetle screen iB_03488

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003033 RA
      MTFIDDYLT EEAKKDSIP EKSPPEGKQ WGYDLYPER 
      RKPDTTPNV SGLLDKKGY ENIQKIKCE RNVYKCIKQ 
      SPIIKLMMG ALKSSGCPV DIRRHISCE ECDPSVSGG 
      FDPVLNQVV VCQNGAKKP AHVQGVLLH EMIHMFDYC 
      RHDLNFRNL DHLACTEIR AANLAHCSF MSAWVQGDA 
      SLFNIKATH GDCVKSKAL SSILATRNV STEEAVDAI 
      ERVFPKCYR DLEPVGRRI RRNSDDMYK AYTEGFYYG 
      YDSV
    CDS sequence
    • >TC003033 RA
      atgactttt atcgacgat tatttaacc gaagaagca 
      aaaaaggat agcatacct gaaaaatcg cctccagaa 
      ggaaaacag tggggctac gacctgtac cccgaacgt 
      cgtaagcct gacacaacc cccaacgtg agcgggctg 
      ctggacaaa aaaggctac gaaaacatt caaaaaata 
      aaatgtgaa cgcaacgtt tacaaatgt atcaaacaa 
      agccccatt attaaatta atgatgggg gcactgaag 
      agctcagga tgccctgtg gacatcaga cggcacatc 
      tcctgcgag gaatgcgac cccagtgta agtggaggc 
      ttcgacccc gtcctaaac caagtagta gtgtgccag 
      aacggggcg aaaaaaccc gcacatgtc caaggcgtt 
      ttgctccac gaaatgatc cacatgttc gattattgc 
      cgccatgac ttgaatttt agaaacctg gaccatttg 
      gcgtgcact gaaattagg gctgccaat ttggcacac 
      tgcagtttt atgtcggcg tgggttcag ggagacgcc 
      tcgcttttc aatattaag gcaacgcat ggtgactgt 
      gttaagagt aaagcgctt agttctatt ctagcaact 
      aggaatgtt tccactgag gaggctgtc gatgccata 
      gaaagggtg tttccgaag tgttatcgc gacctggag 
      cctgtgggg aggcggata cggaggaat tctgatgac 
      atgtacaaa gcctacacg gaagggttc tactacgga 
      tatgatagt gtttag
    mRNA sequence
    • >TC003033 RA
      tcaggaacc gaaatagaa ctgcacatt tttaaatcg 
      gaaatcgaa gtacatgac gtaatagta ctccaaaag 
      ttatgggta tttgtcatg ggacaacca cgttgtaca 
      aatcaccaa aaagatatt ataaataaa aatttaaaa 
      taatagaaa caaaacgtg cagaaaaac acaaatcca 
      gaaataggg cccaaaatg tggcaaatt cagggagca 
      ttccgtaga cacagcccc ctgatatga gctccacca 
      tcatgtcac aaatgatgt aattactag ttccgtaaa 
      atgtaaagt ggggctgta tacagcttc gatttagaa 
      tccgttttg gtgtggtaa tggtgctct gtaaacgat 
      tccgagtaa aattttcaa aattcagtt tttctaacc 
      tcaaagctt ttgcaatgg tagttttct ttctgaatt 
      ctcactttc ttctaagct tgttggatg ctcttaagg 
      tcccattta ccatatttg tagcctaaa attaaaatt 
      taatataat aatataagg aaaaaaccc aagcactat 
      aggttggta gtccttgga gaattcggg cttttcagc 
      tttcgggaa ttcccgaat tctcgtaag cgttcattc 
      ctccaaatc aactctcgc gtcagcgac ctgactctg 
      tttgacatt tgtgacagc tcctaacct ataaaaatt 
      tacaacaca aaaaatgac ttttatcga cgattattt 
      aaccgaaga agcaaaaaa ggatagcat acctgaaaa 
      atcgcctcc agaaggaaa acagtgggg ctacgacct 
      gtaccccga acgtcgtaa gcctgacac aacccccaa 
      cgtgagcgg gctgctgga caaaaaagg ctacgaaaa 
      cattcaaaa aataaaatg tgaacgcaa cgtttacaa 
      atgtatcaa acaaagccc cattattaa attaatgat 
      gggggcact gaagagctc aggatgccc tgtggacat 
      cagacggca catctcctg cgaggaatg cgaccccag 
      tgtaagtgg aggcttcga ccccgtcct aaaccaagt 
      agtagtgtg ccagaacgg ggcgaaaaa acccgcaca 
      tgtccaagg cgttttgct ccacgaaat gatccacat 
      gttcgatta ttgccgcca tgacttgaa ttttagaaa 
      cctggacca tttggcgtg cactgaaat tagggctgc 
      caatttggc acactgcag ttttatgtc ggcgtgggt 
      tcagggaga cgcctcgct tttcaatat taaggcaac 
      gcatggtga ctgtgttaa gagtaaagc gcttagttc 
      tattctagc aactaggaa tgtttccac tgaggaggc 
      tgtcgatgc catagaaag ggtgtttcc gaagtgtta 
      tcgcgacct ggagcctgt ggggaggcg gatacggag 
      gaattctga tgacatgta caaagccta cacggaagg 
      gttctacta cggatatga tagtgttta gctaacatg 
      ttatttatt gttgtacat agaaacatt taataaata 
      ttttaacac gctacattt atcataatt taacttgat 
      acggttggt cacagggtt atttctata agtttacgt 
      tattatcaa tgtagccca caaactcct gtagtttga 
      attttgata aaatctaca attccctga ataagtatc 
      gctcaagat ctatgtttt tataaatta agcacacaa 
      ctgaactac a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY