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Gene information
General information
TC003008 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4765819..4768609
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003008
    iBeetle screen iB_03483

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003008 RA
      MDPEELPEN SDSSDEDYV PDKKEENVS EVESDGDPE 
      SDFSDSENV SGKAKKRRK QSSKPRKKQ KPIEEEEVV 
      NNSAPKQVS EEEKKKLAD DIWADFMKD TGFQSKKDV 
      AKNLPTNSN KPTTVDNAK KTEEKKPET VKITQVFKF 
      AGEEVKVEK EVAKDSIDA RLSNKSSEP GKPVKSKGG 
      LSGILSQLG KKTKISTLE KSKLDWDQF KKEENLQEE 
      LQNHNKGKD GYLERQDFL QRADLRRFE IEKEIRSKE 
      RSKRLNNA
    CDS sequence
    • >TC003008 RA
      atggatcct gaagaactt ccggaaaac agtgacagt 
      agtgatgaa gattacgtt cccgacaaa aaagaagaa 
      aacgtttct gaagttgaa tctgacgga gatccagaa 
      tcggatttt tccgacagt gaaaacgtg agtggcaaa 
      gcaaaaaaa agaagaaaa cagtcatca aaacccaga 
      aagaagcaa aaaccgatt gaagaagaa gaagtagtc 
      aataatagc gcaccgaaa caggtatca gaggaagaa 
      aagaagaaa ctggcggat gatatatgg gccgatttt 
      atgaaagac actggattt caatcaaag aaagatgta 
      gctaaaaat ttgccaaca aatagtaat aagccaaca 
      acagtagac aatgctaaa aaaactgaa gaaaagaaa 
      ccggagacg gttaaaata actcaagtt tttaagttt 
      gctggtgag gaagttaaa gtagaaaaa gaagttgca 
      aaagattca attgacgcg agactatca aataaatct 
      agtgagccg gggaaacct gtcaaaagt aaaggtggt 
      ttaagtgga attttaagc cagttgggg aaaaaaact 
      aaaattagt actttggaa aagtctaag ttagactgg 
      gatcagttt aaaaaagag gagaatctt caggaagaa 
      cttcagaac cataataaa ggcaaggat ggttatttg 
      gaacggcag gattttttg caaagggct gacttacga 
      agatttgag attgagaaa gaaatacgc agcaaggag 
      aggagtaaa agattaaac aacgcctaa
    mRNA sequence
    • >TC003008 RA
      ttggctttt ctcgttaac tgcaaagtt ctagaaact 
      ggagttaca aaaatagaa ttttttatc ggctctgtg 
      tttttgaag tgcattcta atatggcga taacttccc 
      tcgtaatcg gtcgcttgt gctccagct gagttaatt 
      tgtgttttt ttagtaaat tattaagct tgatacact 
      gtccggctt gtacttaat ttgccgaaa aataatcac 
      attttgtgt attttgccg ataagttaa cgataatgg 
      atcctgaag aacttccgg aaaacagtg acagtagtg 
      atgaagatt acgttcccg acaaaaaag aagaaaacg 
      tttctgaag ttgaatctg acggagatc cagaatcgg 
      atttttccg acagtgaaa acgtgagtg gcaaagcaa 
      aaaaaagaa gaaaacagt catcaaaac ccagaaaga 
      agcaaaaac cgattgaag aagaagaag tagtcaata 
      atagcgcac cgaaacagg tatcagagg aagaaaaga 
      agaaactgg cggatgata tatgggccg attttatga 
      aagacactg gatttcaat caaagaaag atgtagcta 
      aaaatttgc caacaaata gtaataagc caacaacag 
      tagacaatg ctaaaaaaa ctgaagaaa agaaaccgg 
      agacggtta aaataactc aagttttta agtttgctg 
      gtgaggaag ttaaagtag aaaaagaag ttgcaaaag 
      attcaattg acgcgagac tatcaaata aatctagtg 
      agccgggga aacctgtca aaagtaaag gtggtttaa 
      gtggaattt taagccagt tggggaaaa aaactaaaa 
      ttagtactt tggaaaagt ctaagttag actgggatc 
      agtttaaaa aagaggaga atcttcagg aagaacttc 
      agaaccata ataaaggca aggatggtt atttggaac 
      ggcaggatt ttttgcaaa gggctgact tacgaagat 
      ttgagattg agaaagaaa tacgcagca aggagagga 
      gtaaaagat taaacaacg cctaagtga cgattctca 
      gtattgctt gtatttatt tatttattt ttacattcc 
      gattgtttt atatcgtgc tcctcaatt gattttaat 
      cattaggtg tgttttttt aatttgact tcgttatag 
      atttttgta tcctcttgt gtaaattaa gacattaaa 
      taaatgaga cagctcaaa gaaatatat ttatttata 
      caacataca gttattatt gtcaaaagt ataggaaaa 
      attgtacaa aaaacaagt aataaagtt gtatagtac 
      acagttctt ttatgtaag aaatgtaaa tatgtacct 
      aaaggttaa caatactaa aaagtgtgc aggaggaag 
      acagttgtt taaggttta tcctaaata taagaaata 
      gtactacgt agttcgtac atcacattt aaatgggtc 
      ctcggcaac tatttgagt ttttgaaag tttgcaagt 
      attaggcca tgattgcaa ctcccataa aacattttt 
      aactgaacg aagtagtcc ctaaagtac tattttttg 
      cctgcagac ctacaaggc ctgtaatgt tggttgcct 
      atcgaaatg ttgtaagaa attacgatt tcagaagtt 
      tattacaaa aatcacttc tctgaaaaa tgtttgctt 
      tgagaaatg gtttgaaca ctaatttaa tttgattct 
      tcgcccgaa taaaattta aaatacggt tcattgact 
      tgtttttaa tatacaaat ataaacata ttttttctt 
      gcgtgctta caagtaatt ataggtcgt tgttcccaa 
      attatacct acaccaccc gtaagtgtt aaaggacat 
      gattttttg ttttgtttt tatttctat aggttttta 
      attagtatt taatcatag aatccatta ctactttaa 
      taagagtca attactgta caaaacaag ttatttatt 
      tgcccgata agcatgata aattattag ggctgttcc 
      agatggaat tttaatttt ttcaatcac tctttgccc 
      acacttatc gtattatta tggctttgt ttataatca 
      aacgtggca gtttatttg cggatctag attttaaaa 
      tttcgttag aaaatatat ttttgaacc aagtaccca 
      cagcacgca tctgttcgt tatgtaacg gcgcaaatt 
      aaaaacgct gtagttttg acgcgatta ttttaagcc 
      aaactgtaa caatttttt tatcctgag taatttttc 
      ttttatttc caatatgcg cctttgaaa aataaagaa 
      agacaattt tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY