Status
Please wait ...
Gene information
General information
TC002986 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5237298..5240695
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002986
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03480

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva dorsal bent - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva cuticle dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number no/almost no eggs -
    vitellarium not present - Number of animals showing the phenotype: 4

    tropharium partiallypresent - Number of animals showing the phenotype: 4
    tropharium partiallyshape irregular - Number of animals showing the phenotype: 4
    egg chamber partiallynot present - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002986 RA
      MEKKKLVTR YIPAGMNLA SLNPQPAKY RTKKCKPIP 
      FDKILERDI FLSEDEDVS TQVVTSPPR KEEKERQKM 
      KPPRTICAN KSLCFDEYN IKITETPGF EKIPSEPPL 
      VKETTEAPV SFKAKMFER LKVTETPAL GKIANEPSL 
      VEETITPPV SFKAKMCER LKVTETPGL GKIASEPSL 
      VVETTSAPV SFKAKMCER LQRHKLKKQ NASPDSTAH 
      SSERQVKIQ KCECSDCDK FASCTFWLH NNVTFDETG 
      KTVTKGLPL LKGDPTDKE ISIFKTFNK IILHGVVIP 
      KPLFSLDET NIDANIQKA MVKINCASS KDVQSYMWP 
      AVQRGLNTF MIAAPKSGK TLAYLPAFC SFAMEKNDR 
      YAELAQYTG PLIIILCPT SKNCNELHD LITELVVEC 
      WDAPVVEVL TENSPVQSD CDILVTVPS VLEECLKAG 
      KVSLKRLCH LAIEDGYFL LKNHNQSME KLLKIINIA 
      QTNRPHNKN IQIVVSSEK WSSDLELLL RKLYVTPLV 
      CFEDFLEAA VYSKVEFKK KKLKSLYKL FYLDDVLKG 
      HYMTHKAVV FCEEEEIGT IKEHLEISG VECITISND 
      LIEDEIFEN EDMWRSLPT GQYKVFLCT DALFERTAR 
      VLNANWVVH YSLPSNWTQ FQKRFRCLV ENLCSPFDV 
      SDNIYKTND KCSFLLIDE ACDNQIFLD FFEVLTRNL 
      VTHLQLK
    CDS sequence
    • >TC002986 RA
      atggaaaag aaaaaattg gtgacgagg tacatccct 
      gctgggatg aatcttgct tctctgaac ccccaacca 
      gcaaaatat agaacaaaa aaatgtaaa ccaatccct 
      tttgataaa atattggaa agggatatt ttcttgtca 
      gaagacgag gatgtgagc acacaagtg gtcactagt 
      cctccaaga aaggaggag aaagagcga caaaaaatg 
      aaaccacca cgcacgatt tgtgcaaac aagtcgctt 
      tgtttcgac gaatataac attaaaata actgaaact 
      ccagggttt gagaaaata ccaagtgaa ccaccactt 
      gtgaaagaa accactgaa gcacctgtt tcctttaag 
      gctaaaatg tttgagaga cttaaagta accgaaact 
      ccagcgtta gggaaaata gcaaatgaa ccatctctt 
      gtagaagaa accattaca ccacctgtt tcttttaag 
      gctaaaatg tgtgagaga cttaaagta actgaaact 
      ccagggtta gggaaaata gcaagtgaa ccatcactt 
      gtggtagaa accacttca gcacctgtt tcatttaag 
      gctaaaatg tgtgaaaga ctccagaga cataaactc 
      aaaaaacaa aacgccagt cctgattcc acagcacat 
      tccagtgaa agacaagtt aaaatacaa aagtgcgag 
      tgtagtgac tgtgataaa tttgcgtct tgtacattt 
      tggctgcat aataacgta acatttgac gaaactggc 
      aagactgtt acaaagggg ctaccacta ctgaagggc 
      gacccgact gataaagag atttcaatt ttcaaaact 
      ttcaataaa atcatactt catggggtg gtcatccca 
      aaaccgctg ttttcttta gatgaaacc aacattgac 
      gccaacatt cagaaagca atggtcaaa ataaattgc 
      gcctcatct aaagacgtc cagtcttac atgtggcca 
      gccgtccaa agaggcctg aacactttt atgatcgct 
      gctcccaaa tcgggaaaa actttagca tatttgccg 
      gcattttgc tcttttgcg atggaaaaa aatgaccga 
      tatgccgag ctggcacag tatacggga cccctaatt 
      atcatatta tgcccaact agtaaaaat tgtaatgaa 
      ttgcatgac ttaatcacg gaactggtc gttgagtgc 
      tgggacgcc cccgtggtt gaagtttta acagaaaat 
      tctccagtt cagagcgac tgtgatatt ttggtaacg 
      gtgccctca gtactagaa gagtgcctt aaagcggga 
      aaagtcagt ttaaagcgg ctgtgccac ctggccata 
      gaagatgga tattttctc ttgaagaac cacaaccag 
      agcatggaa aagttgctt aaaattata aatattgca 
      caaacaaat cgaccccat aacaagaat atccaaatt 
      gtggtcagt tccgagaag tggtcgtct gatcttgag 
      ctgctgtta agaaagctg tacgtgact ccacttgtc 
      tgttttgaa gattttctc gaagcggct gtttactct 
      aaagtggag ttcaagaaa aagaaattg aagtcgttg 
      tataaactc ttttaccta gacgatgtg ctgaaaggg 
      cactacatg acccacaaa gccgttgtg ttttgtgaa 
      gaggaggag atcggaacg atcaaagag cacctggaa 
      attagcggc gtggaatgt attacgatt tctaatgac 
      ctaattgaa gacgagatt tttgaaaat gaagacatg 
      tggcgatcg ttgcccaca ggacaatat aaagtcttt 
      ttgtgtact gatgcccta tttgagagg acggcacgc 
      gtattgaat gccaactgg gtcgttcac tacagcctc 
      ccgtcaaat tggacgcag ttccagaaa cgtttcagg 
      tgtttggtt gaaaatttg tgctcaccg ttcgatgtc 
      agcgacaac atttacaag accaacgat aaatgcagt 
      tttcttcta attgacgaa gcttgtgat aaccagata 
      ttcctagat ttttttgaa gtgttgacc cgcaattta 
      gtgacgcat ttacagtta aagtaa
    mRNA sequence
    • >TC002986 RA
      cgccaaaaa gttgaggtt acgtcgatg tttactttt 
      tttgtacaa aaagaaaat tttaacaag taatattcg 
      tcgggagta aaatttagg cgaattatg gaaaagaaa 
      aaattggtg acgaggtac atccctgct gggatgaat 
      cttgcttct ctgaacccc caaccagca aaatataga 
      acaaaaaaa tgtaaacca atccctttt gataaaata 
      ttggaaagg gatattttc ttgtcagaa gacgaggat 
      gtgagcaca caagtggtc actagtcct ccaagaaag 
      gaggagaaa gagcgacaa aaaatgaaa ccaccacgc 
      acgatttgt gcaaacaag tcgctttgt ttcgacgaa 
      tataacatt aaaataact gaaactcca gggtttgag 
      aaaatacca agtgaacca ccacttgtg aaagaaacc 
      actgaagca cctgtttcc tttaaggct aaaatgttt 
      gagagactt aaagtaacc gaaactcca gcgttaggg 
      aaaatagca aatgaacca tctcttgta gaagaaacc 
      attacacca cctgtttct tttaaggct aaaatgtgt 
      gagagactt aaagtaact gaaactcca gggttaggg 
      aaaatagca agtgaacca tcacttgtg gtagaaacc 
      acttcagca cctgtttca tttaaggct aaaatgtgt 
      gaaagactc cagagacat aaactcaaa aaacaaaac 
      gccagtcct gattccaca gcacattcc agtgaaaga 
      caagttaaa atacaaaag tgcgagtgt agtgactgt 
      gataaattt gcgtcttgt acattttgg ctgcataat 
      aacgtaaca tttgacgaa actggcaag actgttaca 
      aaggggcta ccactactg aagggcgac ccgactgat 
      aaagagatt tcaattttc aaaactttc aataaaatc 
      atacttcat ggggtggtc atcccaaaa ccgctgttt 
      tctttagat gaaaccaac attgacgcc aacattcag 
      aaagcaatg gtcaaaata aattgcgcc tcatctaaa 
      gacgtccag tcttacatg tggccagcc gtccaaaga 
      ggcctgaac acttttatg atcgctgct cccaaatcg 
      ggaaaaact ttagcatat ttgccggca ttttgctct 
      tttgcgatg gaaaaaaat gaccgatat gccgagctg 
      gcacagtat acgggaccc ctaattatc atattatgc 
      ccaactagt aaaaattgt aatgaattg catgactta 
      atcacggaa ctggtcgtt gagtgctgg gacgccccc 
      gtggttgaa gttttaaca gaaaattct ccagttcag 
      agcgactgt gatattttg gtaacggtg ccctcagta 
      ctagaagag tgccttaaa gcgggaaaa gtcagttta 
      aagcggctg tgccacctg gccatagaa gatggatat 
      tttctcttg aagaaccac aaccagagc atggaaaag 
      ttgcttaaa attataaat attgcacaa acaaatcga 
      ccccataac aagaatatc caaattgtg gtcagttcc 
      gagaagtgg tcgtctgat cttgagctg ctgttaaga 
      aagctgtac gtgactcca cttgtctgt tttgaagat 
      tttctcgaa gcggctgtt tactctaaa gtggagttc 
      aagaaaaag aaattgaag tcgttgtat aaactcttt 
      tacctagac gatgtgctg aaagggcac tacatgacc 
      cacaaagcc gttgtgttt tgtgaagag gaggagatc 
      ggaacgatc aaagagcac ctggaaatt agcggcgtg 
      gaatgtatt acgatttct aatgaccta attgaagac 
      gagattttt gaaaatgaa gacatgtgg cgatcgttg 
      cccacagga caatataaa gtctttttg tgtactgat 
      gccctattt gagaggacg gcacgcgta ttgaatgcc 
      aactgggtc gttcactac agcctcccg tcaaattgg 
      acgcagttc cagaaacgt ttcaggtgt ttggttgaa 
      aatttgtgc tcaccgttc gatgtcagc gacaacatt 
      tacaagacc aacgataaa tgcagtttt cttctaatt 
      gacgaagct tgtgataac cagatattc ctagatttt 
      tttgaagtg ttgacccgc aatttagtg acgcattta 
      cagttaaag taaactgtg ttttatctt cggggaaag 
      gcaacacaa tgagatcag aaactctta aactgagga 
      cgtacttta tccggaaac ccaaaaaag tcatcactt 
      ttgagtatg tcacaagta aaagtcatt acgcctaaa 
      cggtctttc ttttgctgt tttagcaat ttctggaga 
      attacacca ataggtgtc ctagttaaa ctaataaac 
      attctagga aaatttcaa caaaattcc gatttgatt 
      attttatta atttactcg ttaccattt tttttgctt 
      tgttcgaac ttttcagtt tgtttttga gccaaaatc 
      ttttgctaa aacagaaac gggtttttc gaaaacctt 
      gttaaagac aagtcaaaa aataaatgc gggataaaa 
      tgcgtatgt agtaatttg tagtaacat aaacacata 
      acatcactt aaactatca cacttacta ctcctagga 
      gcataattt agactgttg taaaaatta aaatagtgt 
      ttttctaag gatttttac gaacttttg tacgttcca 
      tatcctttt ttgatcgtt ttaagtaat tttaaaagt 
      ccagatact gaaactctt cagtaaacc ctgccatga 
      ttcaacaaa aattaagtt gtgacttta tcctcctcc 
      gtggacttt ttgatctca ttatactca cgtttgtta 
      ccatttgat tgtgattga ttaattcat attcaaagc 
      ttgattcgc tttgagtga ctggctgaa tgtttttta 
      tccaagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY