Status
Please wait ...
Gene information
General information
TC002984 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5258437..5289576
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002984
    iBeetle screen iB_03479

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002984 RA
      MVVCQCKGL LKPLRNMCN FPLTYFILG VILVTNGIS 
      AEEKLGCRF PGAPAHSSV VFSDENLGP GAVATYSCE 
      RGFELLGPS RRVCEHGQW LPEGIPFCV LNVAAGKAP 
      MQVSTESGG IPQKAIDGS TSAFFNADT CTLTKAERT 
      PWWYVNLLE PYMVQLVRL DFGKPCCGA NKPATIVVR 
      VGNNRPDLG TNPICNRFT GFLEEGQPL FLPCNPPMP 
      GAFVSVHLE VPVASQLSI CEAFVYTDQ ALPIERCPQ 
      FRDQPPGST ATYNGKCYI FYNRQPMKF RDALTFCRS 
      RGGTLVDES NPALQGFIS WELWRRHRS DASGQYWMG 
      AVRDKQQGG WQWLKGEEV TVSFWNLPV GNGDCARYD 
      GTKGWLWSD TDCNMHLNY ICQHQPKAC GRPEQPPNS 
      TMLAQNYNV GTTIEYICD EGHLLVGPT TRTCLDTGF 
      YNEFPPVCK RIQCGYPAD IANGEYLLV NDSVGYLSR 
      VVYTCDEGY EMIGRAQLA CDIDERWNG PPPRCEPVQ 
      CEPPLEIEN GKFESATNE TLFGSVVEY KCKFGFRLI 
      GPKAITCLA NGQYDALPP KCEAEVLAS TTSPPSISR 
      TPSRTTPRI RTRPPRPSP TTIRADTDS SRLPNKTRP 
      TTTPSSRTP SSSSVPQII VAGHPQDNE IAGSSNIQH 
      GEVPNVNIP LSVDGERRE TFGAKLNLG AVIALGAFG 
      GFVFLAAII TTIVILVRR NRSAKHYRH RASPDCNTV 
      ASFDSSSSE SRNGLNRYY RQAWENLHE SAGSKAGLK 
      AASSNHSPL KRKETLDEP YRNGERHRD GSELVVTEA 
      FSGNKAAEK KRHHHHHHH HDGRQHKDG REWRDPKPP 
      LHRDQKRY
    CDS sequence
    • >TC002984 RA
      atggtcgtg tgtcagtgc aaggggttg ctgaaaccc 
      cttcgaaac atgtgcaat tttccattg acttatttt 
      attttggga gtaattctc gtgacgaac ggaatttcc 
      gccgaagag aaactcgga tgccgcttt cccggcgcc 
      ccggcgcac tcgtcggtg gtgttctcc gacgagaac 
      ctcggcccc ggagccgtg gccacctac tcctgcgag 
      cggggcttc gaactcctg gggccctcc agacgcgtg 
      tgcgaacac gggcagtgg ctgcccgaa ggaatcccg 
      ttttgtgtt ttgaacgtg gctgctggg aaggccccc 
      atgcaagtg agcacagag tccggggga atccctcag 
      aaggccatc gatgggagc acaagcgcg tttttcaac 
      gccgacacc tgcaccctc accaaagct gaacgcact 
      ccctggtgg tacgttaac ctcctggag ccctacatg 
      gtccagttg gtgagactg gatttcgga aaaccctgc 
      tgcggagcg aacaaacct gccacaatc gtcgtccgc 
      gtgggcaac aaccggccc gacttgggc accaacccc 
      atctgcaac cgcttcacc ggcttcctg gaagagggc 
      caacccctc ttcctgccc tgcaacccc cccatgccg 
      ggcgccttc gtcagcgtg cacctggaa gtgcccgtg 
      gccagccag ctctccatc tgcgaagcc ttcgtctac 
      accgaccag gccctgcct attgaacgc tgcccccag 
      ttcagggac cagcccccg ggcagcacc gccacctac 
      aacggcaaa tgctacatt ttctacaac cgacagccc 
      atgaaattc agggacgct ttgaccttt tgcagaagt 
      cggggcggg acgttggtt gacgaaagc aatccggct 
      ttgcaaggg ttcatcagt tgggagttg tggcggcgg 
      cacaggagc gacgccagt gggcagtac tggatgggg 
      gcggtcagg gacaagcag caagggggc tggcagtgg 
      ctgaaagga gaggaggtg accgtttcc ttctggaac 
      ctcccggtt ggaaacggg gactgtgcc aggtatgac 
      ggcaccaaa ggctggctc tggtcggac accgactgc 
      aacatgcat ctgaactac atctgtcag catcagcct 
      aaagcttgt ggaaggccg gagcaaccg ccgaattcg 
      accatgttg gcccagaac tacaacgtg ggcaccacc 
      attgagtac atctgtgac gagggccac ctcctggtg 
      ggccccacg acacgaaca tgtctcgat acagggttc 
      tataacgag ttcccccct gtctgcaaa aggatccag 
      tgcgggtac cctgctgat atagccaac ggagagtac 
      ctcctggtt aatgacagt gtcggttat ctgagccga 
      gttgtgtac acgtgtgat gaaggctat gagatgatt 
      ggaagggca cagcttgcc tgcgatatt gacgaaagg 
      tggaatggg ccaccacca cgttgtgaa cctgtccag 
      tgcgaaccg ccccttgag attgaaaat gggaaattt 
      gaatcggcg accaatgaa acacttttc ggttcggtt 
      gttgagtat aagtgcaaa tttgggttt aggttgata 
      gggccgaag gcgatcacg tgtttggcg aatggacag 
      tatgatgct ctgcctccc aaatgtgaa gctgaagtc 
      ctggcctcc accacctca cccccctcg atctcccgc 
      accccctcg cgaacaact ccccgcatc cgcacgcgc 
      cccccgcga ccatccccc acgaccatc cgcgccgac 
      accgacagc tcccgcctc ccgaacaaa acgcgccca 
      acaaccacc ccttcaagc cgcacgccc agctcttcc 
      tccgtgccc cagatcatc gtcgccggc cacccccag 
      gacaacgag atcgccggc agctccaac atccagcac 
      ggcgaagtg cccaacgtg aacatcccg ctgtccgtg 
      gacggcgag cggcgcgag accttcggc gccaaactc 
      aacctcggc gccgtcatc gccctcggc gccttcgga 
      gggttcgtc ttcctggcg gccatcatc accaccatc 
      gtgatctta gtgcgaaga aatcgaagt gccaaacat 
      taccgtcac cgtgcctcc cccgactgc aacaccgtg 
      gcctcgttc gactcctcc agctcggaa tcgcgcaac 
      ggcttgaac cgctactac cgccaagct tgggaaaac 
      ctgcacgag tccgccggc agcaaagcc ggcctgaag 
      gccgccagt tcgaaccac tcgcccctt aaacgcaag 
      gagacgctc gacgagccc taccggaac ggcgagcgc 
      caccgggac ggctccgag ctggtcgtg accgaagct 
      ttcagcggc aacaaggcc gccgagaag aagcgccac 
      caccatcac caccaccac cacgacggg cgccagcac 
      aaggacggg agggagtgg cgcgacccc aagccgccc 
      ctgcaccgc gaccagaag agatattga
    mRNA sequence
    • >TC002984 RA
      gcgctagtt cttggtcgt ggtaaagtc ggtcggcct 
      gattccaaa ccaccggtc ttcggcttc tgcagtcag 
      tatcgccgg tgagctgac aggtgcaaa accgccgag 
      tgctaacag ttcttcgcc ggtccgcgc gatggtcgt 
      gtgtcagtg caaggggtt gctgaaacc ccttcgaaa 
      catgtgcaa ttttccatt gacttattt tattttggg 
      agtaattct cgtgacgaa cggaatttc cgccgaaga 
      gaaactcgg atgccgctt tcccggcgc cccggcgca 
      ctcgtcggt ggtgttctc cgacgagaa cctcggccc 
      cggagccgt ggccaccta ctcctgcga gcggggctt 
      cgaactcct ggggccctc cagacgcgt gtgcgaaca 
      cgggcagtg gctgcccga aggaatccc gttttgtgt 
      tttgaacgt ggctgctgg gaaggcccc catgcaagt 
      gagcacaga gtccggggg aatccctca gaaggccat 
      cgatgggag cacaagcgc gtttttcaa cgccgacac 
      ctgcaccct caccaaagc tgaacgcac tccctggtg 
      gtacgttaa cctcctgga gccctacat ggtccagtt 
      ggtgagact ggatttcgg aaaaccctg ctgcggagc 
      gaacaaacc tgccacaat cgtcgtccg cgtgggcaa 
      caaccggcc cgacttggg caccaaccc catctgcaa 
      ccgcttcac cggcttcct ggaagaggg ccaacccct 
      cttcctgcc ctgcaaccc ccccatgcc gggcgcctt 
      cgtcagcgt gcacctgga agtgcccgt ggccagcca 
      gctctccat ctgcgaagc cttcgtcta caccgacca 
      ggccctgcc tattgaacg ctgccccca gttcaggga 
      ccagccccc gggcagcac cgccaccta caacggcaa 
      atgctacat tttctacaa ccgacagcc catgaaatt 
      cagggacgc tttgacctt ttgcagaag tcggggcgg 
      gacgttggt tgacgaaag caatccggc tttgcaagg 
      gttcatcag ttgggagtt gtggcggcg gcacaggag 
      cgacgccag tgggcagta ctggatggg ggcggtcag 
      ggacaagca gcaaggggg ctggcagtg gctgaaagg 
      agaggaggt gaccgtttc cttctggaa cctcccggt 
      tggaaacgg ggactgtgc caggtatga cggcaccaa 
      aggctggct ctggtcgga caccgactg caacatgca 
      tctgaacta catctgtca gcatcagcc taaagcttg 
      tggaaggcc ggagcaacc gccgaattc gaccatgtt 
      ggcccagaa ctacaacgt gggcaccac cattgagta 
      catctgtga cgagggcca cctcctggt gggccccac 
      gacacgaac atgtctcga tacagggtt ctataacga 
      gttcccccc tgtctgcaa aaggatcca gtgcgggta 
      ccctgctga tatagccaa cggagagta cctcctggt 
      taatgacag tgtcggtta tctgagccg agttgtgta 
      cacgtgtga tgaaggcta tgagatgat tggaagggc 
      acagcttgc ctgcgatat tgacgaaag gtggaatgg 
      gccaccacc acgttgtga acctgtcca gtgcgaacc 
      gccccttga gattgaaaa tgggaaatt tgaatcggc 
      gaccaatga aacactttt cggttcggt tgttgagta 
      taagtgcaa atttgggtt taggttgat agggccgaa 
      ggcgatcac gtgtttggc gaatggaca gtatgatgc 
      tctgcctcc caaatgtga agctgaagt cctggcctc 
      caccacctc acccccctc gatctcccg caccccctc 
      gcgaacaac tccccgcat ccgcacgcg ccccccgcg 
      accatcccc cacgaccat ccgcgccga caccgacag 
      ctcccgcct cccgaacaa aacgcgccc aacaaccac 
      cccttcaag ccgcacgcc cagctcttc ctccgtgcc 
      ccagatcat cgtcgccgg ccaccccca ggacaacga 
      gatcgccgg cagctccaa catccagca cggcgaagt 
      gcccaacgt gaacatccc gctgtccgt ggacggcga 
      gcggcgcga gaccttcgg cgccaaact caacctcgg 
      cgccgtcat cgccctcgg cgccttcgg agggttcgt 
      cttcctggc ggccatcat caccaccat cgtgatctt 
      agtgcgaag aaatcgaag tgccaaaca ttaccgtca 
      ccgtgcctc ccccgactg caacaccgt ggcctcgtt 
      cgactcctc cagctcgga atcgcgcaa cggcttgaa 
      ccgctacta ccgccaagc ttgggaaaa cctgcacga 
      gtccgccgg cagcaaagc cggcctgaa ggccgccag 
      ttcgaacca ctcgcccct taaacgcaa ggagacgct 
      cgacgagcc ctaccggaa cggcgagcg ccaccggga 
      cggctccga gctggtcgt gaccgaagc tttcagcgg 
      caacaaggc cgccgagaa gaagcgcca ccaccatca 
      ccaccacca ccacgacgg gcgccagca caaggacgg 
      gagggagtg gcgcgaccc caagccgcc cctgcaccg 
      cgaccagaa gagatattg acaaccttg tatgattat 
      aacagtaca attggacgc ctagggaat tttaaatta 
      tttatttat tgctaactg ggatgtgaa gcgagctga 
      tggttttaa ccaaatgtg ggtttggag aaaggactc 
      gttggtggc tatttgagg gtcgcaagt ggcctttga 
      atattctta actagtgta aataattag cagcaggag 
      cgcagaagg atgatggtt gaattcctt ggccttact 
      ttacgagtc aattgtttt agcttaaag ttttattta 
      aagtaaggc taatgtgat tcacagtac aaaagtgaa 
      gagcaatgg acgactgat tcgtatatc tgtgtataa 
      aaatgtagt tctccctga tttatgatt ttaaactta 
      gtttaaggt aacttatta gggaaggta accacttag 
      tgccattgg attaggcaa ctgtaaaat tctacgcaa 
      aaacatacc taggacttt atggcagca tgagtattt 
      tatgcatca tacccaaaa agatagcaa taagtacct 
      ataattagc gtcgtatgt aagataaat gtatcatgt 
      aatatacaa attcaaata ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY