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Gene information
General information
TC002959 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 1227538..1232325
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002959
    iBeetle screen iB_03476
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: all larvae were dead
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not completely molted - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    Comment: all four beatles dead

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    Comment: died with pupal and adult features

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Pupal morphology
    oblique dorsal thoracic muscles partiallyrounded - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002959 RA
      MSDIFQQLE SNNKEEVEA VKQKILDQF LSVKDSWLV 
      NGLYDYYLS TNSANTIVV LINIKEPHH QYLFDRLSD 
      SIAKSSKTD VKVQALTLL GHIVRNQPT WLYRLSEHH 
      LLKDLLRLL KEEVEILPL ISALLVLIV LIPMIPYSM 
      GELLNHVFE IFSRLASWN CNPGRLVEE QMIHMQVAL 
      YALFLRLYG MFPCNFLAY LRTQYKDKN RAVFIHTIK 
      PMLETVKMN PSLVTTSKD NETTSERWR KMAVHDVIV 
      ECERFSLDV SDTFCAHDS CQLASGFRS RSGTANSTI 
      DAVYFQSLK NLAASMSSE KGEYFTPSM VFNPNTPPV 
      GGVIPTYVP QPEFTHDEN VVPQAGSSP PEAAIEATP 
      ETTPILQEE TQIRNQVKP LASPSPQSP LRKEKSPFN 
      FAIEVMHAS LDTKGDLQL SALTFEKSP VGSIKLKSL 
      SPTSPQKIK DRSPTKSSG SSDPEEIPT ERKTETSHQ 
      PALKQCDSV LHESEDSAS ENLEDDRSS PCAAGGLHM 
      PNSKSMKDF AKRVQRLRH HSQCTAESE VQSGSSPEM 
      DSTKVRRAN SCPEMKKSP VSSIKDNVS RTLDETDEE 
      TNLERAESA MSNGVDAMS DVSGNKLAT IETQTDNFW 
      PMPYEHLFL GIFPTLGTE VKSSPGPVT TIGGAQERY 
      SQPSIYEVL DKYVETAIQ DHEKDNIKG LKNQLKLVH 
      QQLLFERHR REIHAFKNR RFLADAKNT QVLEEHNSA 
      LRDQVQLQQ ADIDTLRYQ LERIQRERE LESKMFTNT 
      ITSLEDKCK SLEVQNRAL QEVEAERLE AIESLKTKC 
      MSLTTDKQK AEAMLIDAL AEVKFAKEQ ASAGEKGRA 
      ELENVNKQL VLIGELQLK YKERLDDLS SLRRSDHEV 
      ELLKESYNS EIKGLHQRY REKMTKLEA CTSKISDLE 
      QTIRQNEET ISLQKRTLN SINEETEEK LKVLESKYQ 
      TQLTISRAF EEKILEMYK QLEMSTRKP IRSPDTSSC 
      QEVQVPSDK VSGAVALSQ RSSPLSCSL TSSDGSLAL 
      VQPDMRGLQ ALVDEESPV EVKPNIPKD GDNLPSTSG 
      TKK
    CDS sequence
    • >TC002959 RA
      atgtcagat atttttcaa caattagaa tctaacaat 
      aaggaagaa gtagaagct gttaaacag aaaattctt 
      gaccagttt cttagcgtc aaagattcc tggcttgtt 
      aatggcttg tatgactat tatttaagt acaaattcg 
      gccaacact atcgtcgtg ttgatcaat attaaggaa 
      ccacaccac caatattta tttgatagg cttagtgat 
      tctattgca aaaagttca aaaactgat gtcaaagtc 
      caggctctc actctcttg ggccacatt gtccgcaac 
      caacccacc tggttgtac agactgtca gagcatcat 
      ctcttaaaa gacttgttg agattgcta aaggaagaa 
      gtggagatt ttgccactg ataagtgca ctactcgtt 
      ttaatcgtc ttaatcccc atgattccc tattccatg 
      ggggagctc ctaaaccac gtctttgaa attttcagc 
      cggttggcc tcgtggaac tgcaatcct ggaagacta 
      gttgaagaa cagatgatt cacatgcaa gtcgctctg 
      tatgcttta tttctgaga ctttacggc atgtttcct 
      tgcaatttt ctcgcatat ctaagaaca cagtacaaa 
      gacaaaaac agagcagta tttattcac accattaag 
      ccgatgctt gaaacggtt aagatgaat ccctctctc 
      gtgacgaca tccaaagat aatgagaca acaagcgag 
      aggtggaga aaaatggcc gtccacgat gttatagta 
      gaatgcgaa cgattttct ttggatgtt tctgatact 
      ttctgtgct cacgattct tgtcaactt gcgtcaggg 
      ttccggtct cggtctggc actgccaac tcaaccatc 
      gatgcggtt tattttcaa tcgttgaaa aacttggca 
      gcgtcgatg tccagcgaa aagggcgaa tatttcacg 
      cccagcatg gtttttaac cccaacacg cccccagtc 
      ggaggagtc attcccact tatgtgcca cagccggaa 
      tttacacat gatgaaaat gttgtccca caagcaggg 
      tcgtcgccc cctgaggct gcgatcgaa gccacccct 
      gaaactacc cccatactg caagaagag acacaaatc 
      aggaatcaa gtcaaacct ttagcctcc ccgtcaccc 
      caatcaccc ctacggaag gagaaatcg ccgtttaat 
      ttcgcgatc gaagtcatg cacgcaagt ttagacacg 
      aaaggcgat ttgcagtta tcagccctg acgtttgaa 
      aagtctccc gttggtagt attaaactc aagtcacta 
      tcaccaaca agtccgcaa aaaatcaaa gacaggagt 
      ccgaccaaa agtagcggc tcttcagac ccggaggag 
      atcccgact gagagaaaa accgaaact agtcatcaa 
      cccgcactt aaacaatgc gacagcgtt ttgcacgaa 
      agcgaagac tctgcgagc gagaatttg gaagacgac 
      cgcagctct ccctgcgct gccggaggc ctgcacatg 
      cccaactcc aagtccatg aaggatttc gcaaagcgg 
      gtccagcgt ttacgccac cacagccaa tgcacggcc 
      gagtccgaa gtccagagc ggaagtagt cctgaaatg 
      gacagcacg aaagtccgc cgtgcgaat tcctgtccc 
      gaaatgaag aaaagtcca gtttcgtcg attaaagac 
      aacgtgagt cggactctg gacgaaacg gacgaagaa 
      acaaacctg gaaagagcc gagagtgcc atgtcaaac 
      ggcgtagat gccatgtct gatgtcagc ggcaataaa 
      ctcgcaact attgaaacc cagactgat aatttctgg 
      cccatgcca tatgaacat ttgttcttg ggaattttt 
      ccgacgtta gggactgaa gtcaagtct agtccgggg 
      ccggtgacg acaatcgga ggggcgcaa gagcggtat 
      agtcagcct tccatctat gaagtattg gataagtat 
      gtagagacg gctattcaa gaccatgag aaggataac 
      ataaagggg cttaaaaat cagttgaag cttgtgcat 
      caacaacta ttgtttgaa cggcatagg agagaaata 
      catgctttt aaaaaccgg aggtttttg gccgatgcc 
      aagaacact caagttcta gaagaacat aactcagct 
      ttgcgagat caggtgcag ttacaacag gctgatatt 
      gatactttg aggtatcag ttggaacgc attcagaga 
      gagcgcgag cttgaaagt aaaatgttt acaaatact 
      attacaagc ctggaggat aagtgcaaa tcactagaa 
      gttcaaaac agagcactg caagaagtt gaggcagaa 
      cggctggag gctatcgag agtttaaaa acgaaatgt 
      atgtcgttg actacagac aaacaaaaa gccgaggca 
      atgctcatt gatgctctt gcagaagtc aagtttgcc 
      aaagaacaa gcttcagcg ggcgagaaa ggtcgcgcc 
      gaacttgaa aacgtaaac aaacaactc gttttaata 
      ggagaactc cagctgaaa tacaaggaa agactggac 
      gatctgtcc tcgctccgg cgatctgac cacgaagtg 
      gaattgctg aaggaatcg tacaacagt gaaataaaa 
      ggtttgcac cagcgttac cgcgagaaa atgaccaaa 
      ctggaggct tgcaccagc aaaatctcc gacttggag 
      caaacgatc cggcagaat gaggagacc atctccctg 
      caaaaacgc actttgaac agcatcaac gaagagaca 
      gaggagaaa ctcaaggtt ttggagagc aagtaccaa 
      actcagttg acaataagt cgcgccttt gaggagaaa 
      attcttgaa atgtacaaa cagctggaa atgtcgacg 
      cggaaaccg attcgctcg ccagacacg agcagttgt 
      caggaggtc caagtgccg agcgataaa gtgagcggg 
      gctgtggca ctaagtcag cggtcgtcg cctctgtcg 
      tgctcgttg acttcgtcg gacgggagt ctggcttta 
      gtacagccg gacatgcgg gggttgcag gctttggta 
      gacgaagaa tctcccgtg gaggtcaaa ccaaatatt 
      cccaaggat ggggacaac ctgccttct acttcaggc 
      acaaagaaa tga
    mRNA sequence
    • >TC002959 RA
      gattgtgat aaaaaaatt atggaataa cttttaata 
      tttctgagc tgatttttt gacttaacc tcaccacat 
      gtcatgtca ttcatgtca tgatgactt ctgtcacct 
      tgtcaaaag tcaaaaaac tcgaagtat tttcgaatt 
      aaagagatt acacttttt cgaaaaaac acaaattcc 
      ttaatttaa aatccacca catttacaa ctttttgta 
      aacagatta tggttctgc gcatgccct atgataaaa 
      atgaatcca tttgatgac acttgaatc ccgaacagt 
      gtgaatttc tgcaaattt gcaactaca tttattagt 
      gtaattagg tattttcca aattaaaca aaattacat 
      gacgaaatc tatttgctc gccacaact aaccatcgc 
      tccactgct aaatatagg cactgcgta ttttttgag 
      gatgcactt tagccacag caaaaagtt caactttca 
      cattgttac tgcctaaca tctactttc atcaattag 
      ctaacctaa cactttgac tgatcaaac taccaaaat 
      gtcagatat ttttcaaca attagaatc taacaataa 
      ggaagaagt agaagctgt taaacagaa aattcttga 
      ccagtttct tagcgtcaa agattcctg gcttgttaa 
      tggcttgta tgactatta tttaagtac aaattcggc 
      caacactat cgtcgtgtt gatcaatat taaggaacc 
      acaccacca atatttatt tgataggct tagtgattc 
      tattgcaaa aagttcaaa aactgatgt caaagtcca 
      ggctctcac tctcttggg ccacattgt ccgcaacca 
      acccacctg gttgtacag actgtcaga gcatcatct 
      cttaaaaga cttgttgag attgctaaa ggaagaagt 
      ggagatttt gccactgat aagtgcact actcgtttt 
      aatcgtctt aatccccat gattcccta ttccatggg 
      ggagctcct aaaccacgt ctttgaaat tttcagccg 
      gttggcctc gtggaactg caatcctgg aagactagt 
      tgaagaaca gatgattca catgcaagt cgctctgta 
      tgctttatt tctgagact ttacggcat gtttccttg 
      caattttct cgcatatct aagaacaca gtacaaaga 
      caaaaacag agcagtatt tattcacac cattaagcc 
      gatgcttga aacggttaa gatgaatcc ctctctcgt 
      gacgacatc caaagataa tgagacaac aagcgagag 
      gtggagaaa aatggccgt ccacgatgt tatagtaga 
      atgcgaacg attttcttt ggatgtttc tgatacttt 
      ctgtgctca cgattcttg tcaacttgc gtcagggtt 
      ccggtctcg gtctggcac tgccaactc aaccatcga 
      tgcggttta ttttcaatc gttgaaaaa cttggcagc 
      gtcgatgtc cagcgaaaa gggcgaata tttcacgcc 
      cagcatggt ttttaaccc caacacgcc cccagtcgg 
      aggagtcat tcccactta tgtgccaca gccggaatt 
      tacacatga tgaaaatgt tgtcccaca agcagggtc 
      gtcgccccc tgaggctgc gatcgaagc cacccctga 
      aactacccc catactgca agaagagac acaaatcag 
      gaatcaagt caaaccttt agcctcccc gtcacccca 
      atcacccct acggaagga gaaatcgcc gtttaattt 
      cgcgatcga agtcatgca cgcaagttt agacacgaa 
      aggcgattt gcagttatc agccctgac gtttgaaaa 
      gtctcccgt tggtagtat taaactcaa gtcactatc 
      accaacaag tccgcaaaa aatcaaaga caggagtcc 
      gaccaaaag tagcggctc ttcagaccc ggaggagat 
      cccgactga gagaaaaac cgaaactag tcatcaacc 
      cgcacttaa acaatgcga cagcgtttt gcacgaaag 
      cgaagactc tgcgagcga gaatttgga agacgaccg 
      cagctctcc ctgcgctgc cggaggcct gcacatgcc 
      caactccaa gtccatgaa ggatttcgc aaagcgggt 
      ccagcgttt acgccacca cagccaatg cacggccga 
      gtccgaagt ccagagcgg aagtagtcc tgaaatgga 
      cagcacgaa agtccgccg tgcgaattc ctgtcccga 
      aatgaagaa aagtccagt ttcgtcgat taaagacaa 
      cgtgagtcg gactctgga cgaaacgga cgaagaaac 
      aaacctgga aagagccga gagtgccat gtcaaacgg 
      cgtagatgc catgtctga tgtcagcgg caataaact 
      cgcaactat tgaaaccca gactgataa tttctggcc 
      catgccata tgaacattt gttcttggg aatttttcc 
      gacgttagg gactgaagt caagtctag tccggggcc 
      ggtgacgac aatcggagg ggcgcaaga gcggtatag 
      tcagccttc catctatga agtattgga taagtatgt 
      agagacggc tattcaaga ccatgagaa ggataacat 
      aaaggggct taaaaatca gttgaagct tgtgcatca 
      acaactatt gtttgaacg gcataggag agaaataca 
      tgcttttaa aaaccggag gtttttggc cgatgccaa 
      gaacactca agttctaga agaacataa ctcagcttt 
      gcgagatca ggtgcagtt acaacaggc tgatattga 
      tactttgag gtatcagtt ggaacgcat tcagagaga 
      gcgcgagct tgaaagtaa aatgtttac aaatactat 
      tacaagcct ggaggataa gtgcaaatc actagaagt 
      tcaaaacag agcactgca agaagttga ggcagaacg 
      gctggaggc tatcgagag tttaaaaac gaaatgtat 
      gtcgttgac tacagacaa acaaaaagc cgaggcaat 
      gctcattga tgctcttgc agaagtcaa gtttgccaa 
      agaacaagc ttcagcggg cgagaaagg tcgcgccga 
      acttgaaaa cgtaaacaa acaactcgt tttaatagg 
      agaactcca gctgaaata caaggaaag actggacga 
      tctgtcctc gctccggcg atctgacca cgaagtgga 
      attgctgaa ggaatcgta caacagtga aataaaagg 
      tttgcacca gcgttaccg cgagaaaat gaccaaact 
      ggaggcttg caccagcaa aatctccga cttggagca 
      aacgatccg gcagaatga ggagaccat ctccctgca 
      aaaacgcac tttgaacag catcaacga agagacaga 
      ggagaaact caaggtttt ggagagcaa gtaccaaac 
      tcagttgac aataagtcg cgcctttga ggagaaaat 
      tcttgaaat gtacaaaca gctggaaat gtcgacgcg 
      gaaaccgat tcgctcgcc agacacgag cagttgtca 
      ggaggtcca agtgccgag cgataaagt gagcggggc 
      tgtggcact aagtcagcg gtcgtcgcc tctgtcgtg 
      ctcgttgac ttcgtcgga cgggagtct ggctttagt 
      acagccgga catgcgggg gttgcaggc tttggtaga 
      cgaagaatc tcccgtgga ggtcaaacc aaatattcc 
      caaggatgg ggacaacct gccttctac ttcaggcac 
      aaagaaatg actgatcct cgttcagaa tactgttaa 
      agaggtttg tagttttgg taacaatca cttcatccc 
      ggcgaagtg attggtacg gagaattac agtcacgtg 
      ttaaattga atttgtttt gatgtattt tttaatgta 
      tttaaatat ttttgtcta tacggtaat aaaactact 
      ctacttga