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Gene information
General information
TC002950 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5877953..5882182
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002950
    iBeetle screen iB_03475

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002950 RA
      MLFKNSNKC LSSSPNLIF DEACIDITN LLNETASVL 
      GQYSECHNC DFQNLTTLE PHKNTSIVV NTRYPLELF 
      FVADAKKQC SVKKLLYEH YRYGWNITE KCSNLYIKE 
      PADSAYLPI LTAFIVLFC FGTLWYMVK CIYKNSGRL 
      RGLVSWNAE IESDLGGSS AGTPLVIER TPSIRKHPH 
      RIKSIDVFR GFCIMIMIF VNYGGGKYW FFSHSVWNG 
      LTVADLVFP WFLWLMGVS FAVSLQAKL RRAVPRRQL 
      VIGVMRRSF ILILLGIII NSNQNLQTI GSLRFPGVL 
      QRIGVCYFI VGMLEIIFT KRSEVESVS CIYDVAVAW 
      PQWLCVTVL VVIHTCVTF LGDVPGCGR GYLGPGGLD 
      DNGRFYNCT GGVAGYIDR QVFGEHMHK NPVCKKLYE 
      IDVYFDPEG ILGTLTSVL TVYFGVQAG RTLNTYQNV 
      KAKVIRWVV WGSLAGLLG GALCEFKQN DGLIPLNKQ 
      LWSLSFALV LSGMAFIIQ AFLFVLVDI LRKWGGRPF 
      FYPGMNSLF LYVGHELFK DTFPFAWTP TSETHGAYL 
      LMNLWGTAV WVAIAIFLY KRNVFFAL
    CDS sequence
    • >TC002950 RA
      atgctgttc aaaaatagt aataaatgc ctgtcctca 
      agccccaac ctgatcttc gatgaggct tgcatcgac 
      attactaat ctcctgaac gagacagcc tcggttttg 
      gggcagtat tcggagtgc cacaactgt gattttcaa 
      aatttaacc acgctcgag ccgcataaa aatacttcg 
      attgttgta aacacgcga tatcccctt gagctgttc 
      tttgttgcc gatgccaaa aagcagtgc agcgttaag 
      aagctgtta tatgagcat tatcgctac gggtggaat 
      attacagag aagtgttcg aatttgtat attaaggag 
      cccgcagat agcgcctac ttgccgatt ctcacggct 
      tttatagtg cttttttgc ttcggtacg ctatggtac 
      atggtcaag tgtatttac aaaaattcc gggcgcttg 
      agaggactc gtttcgtgg aatgcagaa atcgaaagc 
      gacttgggt gggtcatcg gcggggact cccctggtc 
      atcgaacga acgccatct atccgaaaa cacccgcac 
      cgtatcaag tccattgat gttttccgc ggcttttgc 
      atcatgatc atgattttc gtgaattac ggcgggggg 
      aagtactgg tttttcagc cattctgtg tggaatggc 
      ctaacagtc gcagacttg gtattcccc tggtttttg 
      tggctgatg ggtgtctcg tttgccgtt tcgttgcaa 
      gcgaaattg aggcgggcg gtgcctagg cgccagcta 
      gtcataggt gtgatgcga cgttcgttc attttaatt 
      ctgctgggg attattata aattcgaac caaaacttg 
      caaaccatc ggctctttg cgattcccg ggcgtgttg 
      cagcgaatt ggcgtctgt tatttcatc gtcggaatg 
      ttggaaatt attttcaca aaacggtcg gaagttgag 
      agcgtctcc tgcatttat gacgtggca gtggcatgg 
      ccccagtgg ctttgtgtc accgttctt gtggtgata 
      cacacttgt gtcacgttt ttgggggat gtgccaggc 
      tgtgggagg ggctatttg gggcccggg gggctcgat 
      gacaacggg aggttttac aactgcacg gggggcgtt 
      gctggatac atcgatcgg caagttttt ggcgaacat 
      atgcacaag aatcccgtg tgtaaaaaa ttgtacgaa 
      attgatgtt tacttcgac cctgaagga atacttgga 
      acgctaact tccgtcttg actgtttat ttcggggtg 
      caggcaggc cgcacactc aacacctat caaaacgtg 
      aaggctaag gtgattcgt tgggtggtt tggggctca 
      cttgcgggc ttgcttggt ggagcgctt tgcgagttc 
      aagcaaaac gacggcttg attccccta aacaaacag 
      ctgtggtcg ctgtctttc gcgttggtt ttgtccggg 
      atggcgttc attattcag gcgtttttg tttgttctt 
      gtggatatt ctaaggaaa tggggagga aggccgttc 
      ttttatcca gggatgaat tcgttgttt ttgtatgtg 
      ggacatgaa ttgtttaag gatactttt ccattcgct 
      tggactccc acgagtgag acgcatgga gcttattta 
      ttgatgaat ctttgggga accgcagtc tgggttgcc 
      attgctatt ttcttgtat aaacgtaac gtatttttt 
      gcactttaa
    mRNA sequence
    • >TC002950 RA
      acagttgat agttggcgg cactgcctg actcaactg 
      tcagataaa taacaaggt tacatttgt caattctca 
      ttaaaatcc aaaacaagg cgaagtgaa ttgtgttat 
      cctgtttgt ttttgtgtg cactaataa ccgttgcat 
      cgcctgtta ttaaaaatc accagtgat cagcaattc 
      ccaacccgt gtgataaga caaaaaatt ttaagcagc 
      agcgatatc acatcctga aatgggcaa tcaagtcga 
      agcgtagtc tccgtgagt cacattctt ggcacgcta 
      agctttcca ttggtgtaa acaacaaaa tgctgttca 
      aaaatagta ataaatgcc tgtcctcaa gccccaacc 
      tgatcttcg atgaggctt gcatcgaca ttactaatc 
      tcctgaacg agacagcct cggttttgg ggcagtatt 
      cggagtgcc acaactgtg attttcaaa atttaacca 
      cgctcgagc cgcataaaa atacttcga ttgttgtaa 
      acacgcgat atccccttg agctgttct ttgttgccg 
      atgccaaaa agcagtgca gcgttaaga agctgttat 
      atgagcatt atcgctacg ggtggaata ttacagaga 
      agtgttcga atttgtata ttaaggagc ccgcagata 
      gcgcctact tgccgattc tcacggctt ttatagtgc 
      ttttttgct tcggtacgc tatggtaca tggtcaagt 
      gtatttaca aaaattccg ggcgcttga gaggactcg 
      tttcgtgga atgcagaaa tcgaaagcg acttgggtg 
      ggtcatcgg cggggactc ccctggtca tcgaacgaa 
      cgccatcta tccgaaaac acccgcacc gtatcaagt 
      ccattgatg ttttccgcg gcttttgca tcatgatca 
      tgattttcg tgaattacg gcgggggga agtactggt 
      ttttcagcc attctgtgt ggaatggcc taacagtcg 
      cagacttgg tattcccct ggtttttgt ggctgatgg 
      gtgtctcgt ttgccgttt cgttgcaag cgaaattga 
      ggcgggcgg tgcctaggc gccagctag tcataggtg 
      tgatgcgac gttcgttca ttttaattc tgctgggga 
      ttattataa attcgaacc aaaacttgc aaaccatcg 
      gctctttgc gattcccgg gcgtgttgc agcgaattg 
      gcgtctgtt atttcatcg tcggaatgt tggaaatta 
      ttttcacaa aacggtcgg aagttgaga gcgtctcct 
      gcatttatg acgtggcag tggcatggc cccagtggc 
      tttgtgtca ccgttcttg tggtgatac acacttgtg 
      tcacgtttt tgggggatg tgccaggct gtgggaggg 
      gctatttgg ggcccgggg ggctcgatg acaacggga 
      ggttttaca actgcacgg ggggcgttg ctggataca 
      tcgatcggc aagtttttg gcgaacata tgcacaaga 
      atcccgtgt gtaaaaaat tgtacgaaa ttgatgttt 
      acttcgacc ctgaaggaa tacttggaa cgctaactt 
      ccgtcttga ctgtttatt tcggggtgc aggcaggcc 
      gcacactca acacctatc aaaacgtga aggctaagg 
      tgattcgtt gggtggttt ggggctcac ttgcgggct 
      tgcttggtg gagcgcttt gcgagttca agcaaaacg 
      acggcttga ttcccctaa acaaacagc tgtggtcgc 
      tgtctttcg cgttggttt tgtccggga tggcgttca 
      ttattcagg cgtttttgt ttgttcttg tggatattc 
      taaggaaat ggggaggaa ggccgttct tttatccag 
      ggatgaatt cgttgtttt tgtatgtgg gacatgaat 
      tgtttaagg atacttttc cattcgctt ggactccca 
      cgagtgaga cgcatggag cttatttat tgatgaatc 
      tttggggaa ccgcagtct gggttgcca ttgctattt 
      tcttgtata aacgtaacg tattttttg cactttaag 
      tcgagtttt tactagtga tattgtaac atttaactt 
      gatcaataa ttattatct tatgactga tttcaaatt 
      tcctaaaat tattagtga gtgaatttt tattttagt 
      aatgtaagg gtttctcgt ttttattca ttatagagt 
      tcgtgattc atgagaata tatagttta tttaaatta 
      ttgggggtt tcattttat taccctttc aaccaatta 
      ttgaaacac aaataacca ctgaaatat gtattttaa 
      ttaaattaa ttaatcttt aaaatatac atataaact 
      tcaataaaa tgagattca taacacgga gacacacag 
      atatgtgtt aaagtacac ttgacaaca atagtaaaa 
      ttagacatg catttgctg agtacttta gccataatt 
      aaaacagaa gcaataatt tttattgca aatagatta 
      caaatggca cgtatgtga ataaatcat tacaaccgt 
      tcctcctaa aagtgataa gagacacaa tcacagtat 
      tacgtttga cacaattat tgagaaaaa acacagctc 
      atccttgaa acttacata aacatttgg ttaaaatgt 
      gtcgttgtg atcagaagg tggtaacaa ttttagaat 
      aatttaata gacatgaat tatttggca cctttctct 
      accttatcc gtcgtaaaa atacaattt tagaatatt 
      taaacgtta ttttttaca atgtaatca tattaatgt 
      ttagattgt cattgtatt tacataata ccacacttt 
      ccctaggtt attaaggtg tataaagaa ataataaat 
      ggtcataca aattgtact tgtctaggt cctattttc 
      agcagtcat gaagtgaga ttttgcatt gctgaacac 
      acggtgttt aagtgcatg tatgcattt aaatgaagg 
      taatcataa aacaaaatt aaataaagt gttcgaaaa 
      aaggtacgg aaattaata ttttatcta gattagtgg 
      tttatattt tgtacaact ttaaacatt aaaataaga 
      agttttata gaaaacatg tagctgcta cagattttc 
      ttagtaata tctttaact tttttcgat cactttgtt 
      tcaagcggt taataagca taccttagt tgatattta 
      taataacaa ataaaatgt atgttcaac a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY