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Gene information
General information
TC002943 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5961140..5971056
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002943
    iBeetle screen iB_03474

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     larva constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax complete not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs segment shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number # of remaining segments decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: in most cases 7 abdominal segments remaining, in some few cases less segments
    abdomen irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002943 RA
      MSAKMEAVK SEDNNDLIL AKAVAMCVL GLASFLIGI 
LPVKLTKLI NIKAVDGDK NLLISLLLC FGGGVLLFT 
TFIHLQPEV RESFMRLES HNKIPSIGS GVPMSELVF 
CVGFFFVYL IEELVHLFL HRKLNDDAL HRSLSIRCS 
NKKRNLTIP RVTLNKFDD GNISYISNS NKELLNSQS 
TINFESHSH SHSHIDGAL KNSFSGLLA VLALSFHAV 
FEGLAVGLE GSVQKVWYL FAAIATHKL VIAFCVGVE 
LVTSKTKLL LVVLYIGTF AVVTPLGIG IGLVLSESG 
NSSEDITSV VLQGMAAGT LLYVVFFEV LARERGNNH 
SGVWQLLAI LLGFAVMFC LQILTGHEH SHGDAHSHG 
HVHHEHHVE VTTQHHHHH EHPHLI
    CDS sequence
    • >TC002943 RA
      atgtcggcg aagatggaa gccgtgaaa tcggaagac 
aataacgat ctaattctg gccaaagcg gtggcgatg 
tgcgtcctc ggcctcgcg tctttcctc atcgggatc 
ctcccggtg aagctgacc aagctcatc aacatcaaa 
gccgtggac ggtgacaag aacttgctg atctccttg 
ctgttgtgc ttcgggggt ggcgtgctg ttattcacg 
acttttatc cacttgcaa ccggaagtg cgggagagt 
ttcatgcgt ctggagtcg cacaacaag atccccagc 
atcgggagc ggggtcccg atgtcggag ctggtgttc 
tgcgtcggc ttcttcttc gtgtacctc atcgaggag 
ctggtgcac ctcttcctg caccgcaag ctcaacgac 
gacgctctg caccgctcg ctgtccatc cggtgcagc 
aacaagaag cgcaacctg accatcccc cgggtcacc 
ctcaacaag ttcgatgac gggaacatc agctacatc 
agcaactcc aacaaggag ctgctcaac tcgcagtcc 
accatcaac ttcgagagc cactcgcac tcgcactcg 
cacatcgac ggcgccttg aagaactcc ttcagcggg 
ctgctggcg gtgctggct ttgagcttc cacgccgtt 
ttcgagggc cttgcggtc gggctcgag ggcagcgtc 
cagaaggtc tggtacttg ttcgcggcc atagccacg 
cacaagttg gtcattgca ttctgcgtg ggagtcgaa 
ctggtgacc tcgaaaacc aagctgctg ctagtcgtc 
ctgtacatc gggacattc gccgttgtc actcctcta 
ggcatcggg atcgggctg gtgctgagc gagtctggc 
aacagcagc gaggacatc acctcggtg gtgctgcag 
ggaatggca gcggggacg ctcctctac gtcgtcttc 
ttcgaggtt cttgccaga gagcgcggc aacaaccac 
agcggggtt tggcagctt ttggccatc ctgctgggc 
tttgccgtc atgttctgc ttgcagatt ttaactgga 
cacgagcac agccacggc gacgcccac tcgcacggc 
cacgtgcat cacgaacac cacgtggaa gttaccact 
caacatcac caccaccac gagcatccc catttaatt 
tag
    mRNA sequence
    • >TC002943 RA
      ttagcattg ccgcgaccg ctctcttga aacatgtga 
ggatgaact gttttgtta attactaat taatatcgc 
gcgaaaaat tagtactgt gcggaaatt tacgaacgc 
catttcagg agcagcacg agcgccttg gggccatga 
agagctaat cacttcgaa ccgagcaag tgccaagtg 
aagtgtaaa atgtcggcg aagatggaa gccgtgaaa 
tcggaagac aataacgat ctaattctg gccaaagcg 
gtggcgatg tgcgtcctc ggcctcgcg tctttcctc 
atcgggatc ctcccggtg aagctgacc aagctcatc 
aacatcaaa gccgtggac ggtgacaag aacttgctg 
atctccttg ctgttgtgc ttcgggggt ggcgtgctg 
ttattcacg acttttatc cacttgcaa ccggaagtg 
cgggagagt ttcatgcgt ctggagtcg cacaacaag 
atccccagc atcgggagc ggggtcccg atgtcggag 
ctggtgttc tgcgtcggc ttcttcttc gtgtacctc 
atcgaggag ctggtgcac ctcttcctg caccgcaag 
ctcaacgac gacgctctg caccgctcg ctgtccatc 
cggtgcagc aacaagaag cgcaacctg accatcccc 
cgggtcacc ctcaacaag ttcgatgac gggaacatc 
agctacatc agcaactcc aacaaggag ctgctcaac 
tcgcagtcc accatcaac ttcgagagc cactcgcac 
tcgcactcg cacatcgac ggcgccttg aagaactcc 
ttcagcggg ctgctggcg gtgctggct ttgagcttc 
cacgccgtt ttcgagggc cttgcggtc gggctcgag 
ggcagcgtc cagaaggtc tggtacttg ttcgcggcc 
atagccacg cacaagttg gtcattgca ttctgcgtg 
ggagtcgaa ctggtgacc tcgaaaacc aagctgctg 
ctagtcgtc ctgtacatc gggacattc gccgttgtc 
actcctcta ggcatcggg atcgggctg gtgctgagc 
gagtctggc aacagcagc gaggacatc acctcggtg 
gtgctgcag ggaatggca gcggggacg ctcctctac 
gtcgtcttc ttcgaggtt cttgccaga gagcgcggc 
aacaaccac agcggggtt tggcagctt ttggccatc 
ctgctgggc tttgccgtc atgttctgc ttgcagatt 
ttaactgga cacgagcac agccacggc gacgcccac 
tcgcacggc cacgtgcat cacgaacac cacgtggaa 
gttaccact caacatcac caccaccac gagcatccc 
catttaatt tagtttaat ttatttttt tactttttt 
ataattggc aattgattt tgtttgtta agcgtacct 
gaaaattac gaatgtaga aaccttggc attatttct 
gtaaatagt gttaatgcc attatcatg taaatattt 
taaatgaca gcaataaca ttctaataa ttaggtgcc 
aacaaaagc atgcagtac ttatactct tcttcacac 
attttaatt gatacaaca tcccgattc ccatgtttt 
ctcacgtta cataatgaa taatggatt caatcagtt 
cttgtatgt aaatattgt aaaattagc aataaattt 
gtataaaaa at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY