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Gene information
General information
TC032396
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002941,TC002942
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 1415713..1420807
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032396
    iBeetle screen iB_03473
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032396 RA
      MSSTGEGND TVTAKAVAM GVLFAASMT LGTIPIKLS 
      EWFDWKSGT KNSVYVQML LSLGGGVLL CTTFLHLLP 
      EVIEGIENQ ESFTKYQVL PVAEFLMCA GFFVMYFVE 
      ECVHFYLHQ REQNFHIKS ARSSISVSR GQLDSKSDE 
      DLVPPSDVV IPEQGYQQY HHHIDHSHV DHSHMVIED 
      TTIISIRGF LVVLALSVH ELFEGLSVG LESSSSNVW 
      YMFGAVSAH KLVIAFCIG IELVTSGMK TMLVVIYVF 
      VFAVVSPLG IGIGIAVTE ESESSTTVV SVILQGLAS 
      GTLLYQQPD QFSASTSAM TSDKTITAK SVAMCVLFT 
      ASLTLGTLP ILLSRIFRW SSDAQQNPY VRKLLCLGG 
      GVLLCTTFM HLLPEVSER IEKIDSIPT KDVHYAELL 
      MCAGFFTMY FVEECVHAC LHHHHHKDE SVLARTLSV 
      RRGDKDPEK SKTNSARTT VEGPITVCV EDKKHMHDH 
      SHGHSHFVP DTEDSTMVT IRGLFVVLA LSVHELFEG 
      LAVGLESSS QNVWYMFGA VSAHKLVIA FCIGVELVS 
      SGLRTAVVI LYVFIFAVV SPLGIGMGI VISTEGQSA 
      TELPSVFLQ GIASGTLLY VVFFEILSS ERKGGLGQF 
      LSVIVGFLI MLTIKIFED
    CDS sequence
    • >TC032396 RA
      atgtcttcc accggcgaa ggaaacgat acagtaacc 
      gcaaaagca gtagcaatg ggcgtgctt tttgccgca 
      tccatgact ctcggtacc attcctata aaactatcc 
      gaatggttc gactggaaa tctggtacc aaaaacagc 
      gtttacgtc caaatgctc ctctctcta ggcggcggt 
      gttctatta tgcacgacc tttttgcat ttgcttcca 
      gaagtgatt gaaggcatt gaaaatcag gaaagcttc 
      acaaagtac caagtcctc cctgttgct gagtttctc 
      atgtgcgct ggctttttc gtcatgtat tttgtggaa 
      gaatgcgtc cacttttac ctgcatcag agagaacag 
      aattttcat ataaaatca gctcgcagt agcatttct 
      gtgagcagg ggtcaactg gattcgaaa agtgacgag 
      gatctggta ccaccttcg gatgtggta atccccgaa 
      caagggtac cagcagtac caccaccac atcgatcac 
      agccatgtg gatcatagc cacatggtg atcgaggac 
      acgacgatt atctcaatt agggggttt ttggtggtt 
      ttggctctg tcagtccac gagttattt gaaggcctg 
      tcggtgggt ttggagtct tctagcagt aatgtgtgg 
      tacatgttt ggggctgtc agtgcccat aaactagtc 
      attgcgttc tgtattggc atcgaattg gtcacttcg 
      ggcatgaag acgatgctg gtggtgata tatgtattt 
      gtttttgcc gtggttagt cctttgggg attgggatc 
      gggattgcg gttactgaa gaaagcgaa tcttcgacc 
      acggtcgtg tctgtgata ttgcaagga ttggcgagc 
      gggaccttg ctctaccag cagcccgat cagttcagt 
      gcctccacc tccgcaatg accagtgac aaaacaatc 
      accgccaag tcggtggcg atgtgcgtc ctcttcacc 
      gcctcgctc accctcggc accctcccg atcctcctc 
      tcccgaatc ttccgctgg agctccgac gcccagcag 
      aacccctac gtgcggaag ctgctctgt ctgggcggc 
      ggcgtcctc ctctgcacc accttcatg cacctcctg 
      cccgaggtg tccgagagg atcgagaaa atcgacagc 
      atccccacc aaggacgtc cactacgcc gagctcctc 
      atgtgcgcc ggcttcttc accatgtac ttcgtggag 
      gagtgcgtc cacgcctgc ctgcaccac caccaccac 
      aaggacgag tccgtgctt gccaggacc ctctcggtg 
      cggcggggg gacaaggac ccggaaaag tccaagacc 
      aacagcgcc aggaccacg gtcgaaggc cccatcacc 
      gtctgcgtc gaagacaag aagcacatg cacgaccac 
      agccacggc cacagccac ttcgtccca gacacggaa 
      gactcgacc atggtgacc atcagaggc ctgttcgtg 
      gttttagct ctgtcagtc cacgagtta tttgaaggt 
      ctggcagtg ggtttggag tcctcgagt cagaatgtt 
      tggtacatg tttggggct gtcagtgcc cataaactt 
      gtcattgcc ttctgtatt ggggtagag cttgtctct 
      tccgggttg aggaccgca gtggtcata ctttacgtt 
      tttattttc gcggttgtt agtccctta gggatcggg 
      atggggatt gttatcagt acggaaggg caatcagcc 
      actgagttg ccttcggtg tttttgcaa gggatcgcg 
      tcagggaca ctgctgtat gttgtcttt ttcgaaata 
      ttgagctcc gagaggaaa ggtgggctt ggacagttc 
      ctttccgtt atcgtaggg tttcttatc atgctcact 
      attaaaatt ttcgaagac tga
    mRNA sequence
    • >TC032396 RA
      acttactcc tatttcaaa ataaggtaa cacacaacc 
      aaaatagta actagatag ttaagataa aatgtcttc 
      caccggcga aggaaacga tacagtaac cgcaaaagc 
      agtagcaat gggcgtgct ttttgccgc atccatgac 
      tctcggtac cattcctat aaaactatc cgaatggtt 
      cgactggaa atctggtac caaaaacag cgtttacgt 
      ccaaatgct cctctctct aggcggcgg tgttctatt 
      atgcacgac ctttttgca tttgcttcc agaagtgat 
      tgaaggcat tgaaaatca ggaaagctt cacaaagta 
      ccaagtcct ccctgttgc tgagtttct catgtgcgc 
      tggcttttt cgtcatgta ttttgtgga agaatgcgt 
      ccactttta cctgcatca gagagaaca gaattttca 
      tataaaatc agctcgcag tagcatttc tgtgagcag 
      gggtcaact ggattcgaa aagtgacga ggatctggt 
      accaccttc ggatgtggt aatccccga acaagggta 
      ccagcagta ccaccacca catcgatca cagccatgt 
      ggatcatag ccacatggt gatcgagga cacgacgat 
      tatctcaat tagggggtt tttggtggt tttggctct 
      gtcagtcca cgagttatt tgaaggcct gtcggtggg 
      tttggagtc ttctagcag taatgtgtg gtacatgtt 
      tggggctgt cagtgccca taaactagt cattgcgtt 
      ctgtattgg catcgaatt ggtcacttc gggcatgaa 
      gacgatgct ggtggtgat atatgtatt tgtttttgc 
      cgtggttag tcctttggg gattgggat cgggattgc 
      ggttactga agaaagcga atcttcgac cacggtcgt 
      gtctgtgat attgcaagg attggcgag cgggacctt 
      gctctacca gcagcccga tcagttcag tgcctccac 
      ctccgcaat gaccagtga caaaacaat caccgccaa 
      gtcggtggc gatgtgcgt cctcttcac cgcctcgct 
      caccctcgg caccctccc gatcctcct ctcccgaat 
      cttccgctg gagctccga cgcccagca gaaccccta 
      cgtgcggaa gctgctctg tctgggcgg cggcgtcct 
      cctctgcac caccttcat gcacctcct gcccgaggt 
      gtccgagag gatcgagaa aatcgacag catccccac 
      caaggacgt ccactacgc cgagctcct catgtgcgc 
      cggcttctt caccatgta cttcgtgga ggagtgcgt 
      ccacgcctg cctgcacca ccaccacca caaggacga 
      gtccgtgct tgccaggac cctctcggt gcggcgggg 
      ggacaagga cccggaaaa gtccaagac caacagcgc 
      caggaccac ggtcgaagg ccccatcac cgtctgcgt 
      cgaagacaa gaagcacat gcacgacca cagccacgg 
      ccacagcca cttcgtccc agacacgga agactcgac 
      catggtgac catcagagg cctgttcgt ggttttagc 
      tctgtcagt ccacgagtt atttgaagg tctggcagt 
      gggtttgga gtcctcgag tcagaatgt ttggtacat 
      gtttggggc tgtcagtgc ccataaact tgtcattgc 
      cttctgtat tggggtaga gcttgtctc ttccgggtt 
      gaggaccgc agtggtcat actttacgt ttttatttt 
      cgcggttgt tagtccctt agggatcgg gatggggat 
      tgttatcag tacggaagg gcaatcagc cactgagtt 
      gccttcggt gtttttgca agggatcgc gtcagggac 
      actgctgta tgttgtctt tttcgaaat attgagctc 
      cgagaggaa aggtgggct tggacagtt cctttccgt 
      tatcgtagg gtttcttat catgctcac tattaaaat 
      tttcgaaga ctgaaatgt acaagcgac ttaaatgtt 
      ctaaaaatc tctgtatgt gatactatg tggaaagat 
      atttgatgt gaatgttga cacttttta agattagtt 
      ttatgtact taacaaatt attttgtaa cataaaata 
      aatagaatt aggtatc