| General information | |||
| TC002938 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 6004787..6013508 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC002938 | ||
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03472 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC002938 RA
MNPEKPKPE VPAGAGSSW RNSTDNARG TPKYFGPRR TLGQRWIPP SGSKTNNQD ATVQLRQTK SMGFKFLED LSNNDPEKI ILVLMKERE GLLRLLERE VSNDVIVLL ISILRKVCF SSFDETKYS VLQSTLNEK FTNKIKTFI ACLPTQDKF QRRTNSFFW KNPVEFWMN LTDICQDLL NTTPTFACK NLPVVLNAI KIYAPIIEA EQQIEVSDE VKRKVEKML QVMQTLKES EEKEKSKQD EAATTNEPP DDFRQISVY PTPAEIVGG RGFLRENIV KGPYQSVNH YLDVQFRLL REDFVGPLR DGINNYRSS RPSNEIENV KIHKDVYFL KTETINEFY CLRTQFSSK KKKINFEQS KRFMFGSLL CFTKDNFQT LIFGRVAQR KIEDLDKGE VIVAFDEKI KVDFRAAYL MVECSVYFE PYFHVLTVL KGMTEEMFP MERYIIRVH PSVQPPAYL TAPNCKYNI EDYRFNPLS EWPHEDFYG LNDSQMVAF KAAITQEIA VIQGPPGTG KTYLGLKIA QTQLENMDC WYNHTPMLV ICFTNHALD QFLEGLLPF TKEIIRVGG RSQNEKLDS YNLRNNTQR DSAVSEKRF QVQLCLREI KRITEHLNQ IEYYNRVLS FRAFREIPG FSDSWFVHA SNREVVHWL LQGADYGQD LSSIIDNLQ VMQAQEEMY RIGINDNDE TLGYDFNEH ISTIVDSTF DVTVVSHKD DYIITLDSM RNYLQQYKD NLHDLQQIQ NPSLQDLIR EEELHYQIM VLQNKYDYV KHQLSDWKN IQGDIEPPQ NIDLHEPYN MKFEDRWRL YFHWLNLHK QRESDVLRM TCEDFPAIY KEYEVMREM EDVQVMKKA LVVGMTTTS AARLRSSLQ TLKSPIVIV EEAAEVLEA HIVTSITKH CQHLILIGD HKQLKPNTA NYSLEKQYH LGISLFERM IRNNIHCYT LNVQHRMRP EISSLIRPT IYDFLEDHP SVYNRPKIS GVDNCVFFI DHTHAEEEC EGLSKTNLH EVSFFIYLA RHLILNGYN PANITILAA YLGQFFAFQ REKREHKDL LKDVRVAVL DNYQGEESD IILLSLVRN NNENKIGFL KIENRVCVA LSRARNGLY IMGNMTQLC FENKLWRKI KASLEQQKA LGTELPLRC QIHQDQVTH VKSESDFFS VSEGGCNKK CGAELKCGH NCNYLCHIR DRSHMIYKC QAECKKNLC DKDPAHLCK KMCFEDCGL CDYLVHRVL ACGHEANIQ CHIDPEIYK CEILVSTEL PCGHMADKP CFSNPVTFR CPHICDVQV QPCGHACEL LCHIRKDPD HLEYKCQKP CTRFMKYCT VNDEDHRCR KKCFEDCDV CTVVVRKKR TVCNHYYDV RCCEDVDDI DCEKPCAKM RNCGHPCKR KCNEPCQEC QIKVTKIHP ICNHPVKMK CCEKPDRKY CNKNCPLTL PCGHPCTKK CNEPCTPAC GVLVDCSGT EAECGHLVT RMECHMATS SVDPKRLIH FCNAPCKQE LKCGHLCSG SCGECHQGR IHKRCSQKC GAPLVCDHP CDIPCRQAC RPCRRVCSY TCGHSICKK KCGEVCTSC KEPCLRRCP HQWCTKLCG ERCSVPPCT QPCQKALKC GHLCIGFCG DPCPPKCLV CDKEALLEV FFGTESEDA RYVLLNDCK HIVENEGME HWLKQNEFQ ISYKLCPMC KTAIKTTQR YRDYIKMAI KDVAQVKIK ANGSPAEIR EKTDKIKET LQALETKCR LLKMYCGPI TDVLRSLKS RIQQSNKGK RQHLNIFDV QSLMAKLQI VVHIANICC NEKVIVETL HEIFFPQVS LIVEILMRD VDITNQETS DLSYEINRL SRIVDFSCL QKTPQFSYH KANNATAKS LISTVENKL FSCQKFTNE TNDLLKDCL RELNDVMKS GLAISDQER REILQAMGF SKGHWYKCP NGHVYAIGE CGGAMQEAT CNECGARIG GSQHRLLEN NAVATEMDG AVHGAWTIA TNFHNYAL
- >TC002938 RA
atgaatccc gaaaaacca aaacctgaa gttccagca ggggccgga agcagttgg agaaattcc acagataat gccagaggc acgcctaaa tacttcggc ccaaggaga actcttggc cagcggtgg ataccaccg agtgggtcc aaaactaac aaccaggat gcaacagtt cagttgaga caaacaaaa tcaatggga tttaagttc cttgaggat ttatcaaac aacgatccc gagaaaata atattggtg ttgatgaag gaacgtgag ggcctttta cgtttattg gaaagagaa gttagcaac gacgttatt gtcctttta atctcaatt ctgcgcaaa gtttgcttc tcttcattc gatgaaaca aaatacagt gtactccag tcaaccctc aatgaaaaa ttcactaat aaaataaaa acattcatt gcttgcttg cccacacag gacaaattc cagcgccgc acaaactca tttttctgg aaaaaccct gtcgaattt tggatgaat ttgaccgat atttgccaa gatttgtta aacacgacc ccaactttt gcttgtaaa aacctacca gttgtactt aacgcgatt aaaatttac gcaccaatc atcgaggct gagcaacaa atagaggtg tcagacgaa gttaaaaga aaagttgaa aaaatgtta caagtgatg caaacgctt aaagaaagc gaggagaaa gagaaatcg aagcaagac gaagcagcg accactaac gaacctcca gacgacttc cgccaaatt agtgtttat ccaacccct gccgagatc gtgggaggg cgaggtttc ctccgggaa aacatcgtc aaaggcccc taccaaagc gtaaaccac tatttagac gtccagttt cggctctta cgcgaggat ttcgtgggc cctttacga gacggcata aacaactat cgatcatca agaccatca aacgaaatc gagaacgtg aaaattcac aaagacgtc tacttcctg aaaacggaa accataaac gagttttat tgcctccga actcagttc agctccaag aagaaaaag atcaatttt gagcagtcg aaacgcttc atgtttggg tcgttgctg tgcttcacg aaagacaac tttcaaact ttgattttc gggagagtt gcccaaaga aaaatcgag gacttggat aagggcgaa gtaatcgtc gctttcgac gaaaaaatc aaggttgat ttcagggct gcttacctg atggttgaa tgtagcgtc tacttcgag ccgtatttt cacgtcctc actgttttg aaaggcatg actgaggag atgtttccg atggagagg tacattatc cgggttcac ccgtccgtg caaccccct gcatattta acagccccc aactgcaag tacaacatt gaagactat cgatttaac cctttgtca gagtggccc cacgaggac ttttacggt ttgaatgac tcccagatg gtggcgttc aaagcggca atcacccag gaaatcgcc gttattcag ggaccccca gggactgga aaaacgtat cttggacta aagatagca caaacgcaa ttggaaaac atggactgt tggtacaat catacaccc atgctggtg atttgtttc acgaaccac gcacttgac cagtttttg gagggtttg cttccattt acaaaagag attatacga gttggtgga cggtcccaa aacgagaaa ctggattca tacaatttg agaaacaac actcagcga gatagcgct gtttcagaa aaacgtttc caagtacag ctctgttta agagaaata aaacgcatc actgagcat ttgaatcag atcgaatat tacaacagg gttttgtct tttcgtgct tttcgagaa attccgggt ttttctgat tcgtggttt gtacatgcg tccaatcga gaggtagtt cactggtta ctccagggc gcagattac gggcaagat ttgtccagt ataattgac aatttgcaa gttatgcag gctcaggag gaaatgtac agaattggc atcaacgac aatgatgag actcttgga tacgacttt aatgaacat ataagtaca attgttgat tcgacgttt gatgttact gttgtatca cataaagat gattacata attaccttg gactccatg cgcaattac ttgcagcag tacaaagac aacctacat gacctacaa cagatacaa aacccatca ctccaggat cttatacga gaagaagag ttgcattac caaataatg gttttgcaa aacaaatac gactacgtc aaacaccaa ttgagcgac tggaaaaac atccaaggt gacatcgag ccaccacag aatatagat ttgcatgag ccctacaat atgaagttt gaagacaga tggaggctt tattttcac tggctgaat ttgcataaa cagcgtgaa tcagacgtt ctgcggatg acttgtgag gattttccg gcgatttat aaggagtat gaagtaatg cgagaaatg gaagacgtg caagtaatg aaaaaagcg ttagtagtg ggaatgacg accaccagt gcagctcga ttaagatcg tccttgcaa actctgaaa agcccgatt gttattgta gaggaagcg gctgaagtt ctggaagcc cacattgta acctcgatc accaaacac tgccaacac ttgattttg atcggggac cataagcag ctgaaacca aacacggcc aattatagc ctggaaaag cagtaccac ctgggaatc agtctgttc gaaagaatg attcgaaac aatattcac tgttacacc cttaacgtg cagcaccgc atgcgaccg gaaatttcg tctctgatc cggcccaca atttacgat tttttggaa gaccatccc tcggtgtac aacagaccg aaaatatca ggcgtcgac aactgcgta tttttcatc gatcatacg cacgctgag gaagagtgc gagggtttg agcaaaacc aacttgcac gaagtctcg ttttttatt taccttgca aggcatttg attttgaat ggttacaac cctgcaaac atcacgatt ctggcggct tatttgggc cagtttttt gcgttccag agggagaag agggagcat aaggacttg ctgaaggac gtcagggtt gctgttttg gataattac caaggagag gagtcggat atcattctt ctttcgtta gttcgaaat aacaatgaa aataaaatt ggttttcta aaaatcgaa aaccgtgta tgcgtcgct ctttcgagg gccaggaat gggttgtat ataatggga aatatgacg caactgtgt tttgaaaat aaactttgg cgcaaaatc aaagcgagt cttgagcaa caaaaggct ctcgggact gaattacct ctcaggtgt caaattcac caggaccaa gtcacgcat gtgaaatct gagagtgat ttcttttcg gtttctgaa ggcggttgc aataaaaag tgtggcgcg gagctgaag tgtggtcac aactgcaac tacttgtgc cacattcgg gacaggagc cacatgatt tacaaatgc caagcagaa tgtaaaaag aatttgtgc gacaaagac ccagcacat ctctgcaag aaaatgtgt tttgaagat tgcggtctt tgcgattac ctagtccac agagtttta gcttgcggt cacgaagct aacatccaa tgccacatt gatccagaa atctacaaa tgcgaaatt ttggtttca accgaattg ccttgtggc cacatggct gacaaaccc tgcttttcc aatcctgta actttcagg tgtccacac atttgcgat gttcaagta cagccttgt ggtcatgca tgcgaatta ttatgccac atccgtaaa gatcctgac catttagaa tataagtgt cagaaacct tgcacccga tttatgaaa tattgcacc gtcaatgat gaggatcat agatgcagg aagaagtgt ttcgaagac tgcgacgtt tgcacagtt gttgtaaga aagaagaga actgtgtgc aaccactat tacgacgtc cgttgctgt gaagatgtg gacgacatc gactgtgaa aaaccttgt gctaagatg cgaaactgt ggccaccct tgcaaacgc aagtgcaat gaaccttgc caagagtgc caaatcaag gtgactaaa atccacccc atctgcaac catcccgtg aagatgaaa tgctgcgag aagcctgat cgcaagtac tgcaacaaa aactgtcct ttgacactg ccttgtggc cacccttgc accaaaaag tgtaacgag ccctgcacc cccgcctgt ggtgtcctg gtggactgt tcgggcacc gaagcggaa tgtggccac ctggtgacc agaatggag tgccacatg gccacatct tccgtcgac cccaaacgt ctaatacac ttctgcaac gctccctgc aaacaagag ctaaaatgt ggccatttg tgctcgggc tcctgcggt gagtgccac caaggccgc atccacaag cgatgctcg caaaaatgt ggggccccg ttggtgtgc gaccaccct tgcgatatc ccctgcaga caggcctgc agaccctgc agacgggtc tgttcctac acttgtggg cacagcatt tgcaaaaag aagtgcggc gaagtctgc acgtcttgc aaagagccg tgtttgagg cggtgcccc caccagtgg tgcactaag ctgtgtggg gagcgctgc agtgtgccc ccatgcacg caaccgtgc cagaaggcg ctcaaatgt gggcacttg tgtattggt ttttgtggg gatccgtgt ccgccaaag tgtttggtg tgcgacaag gaggcgttg cttgaagtg ttctttgga acggaaagt gaagacgcc agatatgtt ctactgaac gactgcaaa cacattgtt gaaaatgag ggcatggag cactggctc aaacaaaac gagtttcag attagttac aaattgtgt ccaatgtgc aaaactgcg attaaaacc actcagcgg taccgggat tacatcaaa atggccatc aaagatgtg gctcaagtg aagatcaaa gcgaacggg agtcctgcc gaaatcagg gaaaaaact gacaaaatc aaagaaact ttacaagcc ttggaaacc aaatgtaga ttacttaaa atgtactgc ggacccatc acagacgtg ttgaggtcc ctcaagtca cgaattcaa cagtccaac aagggcaag agacaacac ttgaacatt ttcgatgtc cagtccctt atggcgaag ctgcaaata gtagtccac attgctaac atttgctgc aacgagaaa gtaattgtg gaaacactg catgaaatt tttttcccg caagtgtcc ctcattgtc gaaattctg atgcgagac gtggacata acaaatcaa gaaacaagc gatttgagt tacgaaatc aacagactg tcgcgaatt gtggatttc agctgcctt caaaaaacc ccacaattt tcctatcat aaagccaac aatgcgact gccaaatca ttgataagc actgtggaa aataagctt ttttcttgc cagaaattt acaaacgag accaacgat ttgttgaag gattgttta agggaattg aacgatgtt atgaaatcg ggactggct atttcggat caggaaaga agagaaatt ttgcaggct atgggtttt agtaaaggg cactggtac aagtgtccc aatgggcac gtctatgct attggtgaa tgtgggggc gctatgcag gaagcaacg tgcaatgag tgcggggct cgaattgga ggctcccag catagactt ctggagaat aatgctgtg gccactgaa atggatggg gctgttcat ggggcttgg accatcgca actaatttt cataattat gctctatga
- >TC002938 RA
ataaaagta cattggttc aaatgcaat ttggtaaca cgtcatcgt cattgtctg gataaagtg tgttgcaac gtgcaatat taattttac ctttgtctt tgtcttgat gagccttgt actttgtta cttaattat tacctgcta ttaatttgg tatgaatcc cgaaaaacc aaaacctga agttccagc aggggccgg aagcagttg gagaaattc cacagataa tgccagagg cacgcctaa atacttcgg cccaaggag aactcttgg ccagcggtg gataccacc gagtgggtc caaaactaa caaccagga tgcaacagt tcagttgag acaaacaaa atcaatggg atttaagtt ccttgagga tttatcaaa caacgatcc cgagaaaat aatattggt gttgatgaa ggaacgtga gggcctttt acgtttatt ggaaagaga agttagcaa cgacgttat tgtcctttt aatctcaat tctgcgcaa agtttgctt ctcttcatt cgatgaaac aaaatacag tgtactcca gtcaaccct caatgaaaa attcactaa taaaataaa aacattcat tgcttgctt gcccacaca ggacaaatt ccagcgccg cacaaactc atttttctg gaaaaaccc tgtcgaatt ttggatgaa tttgaccga tatttgcca agatttgtt aaacacgac cccaacttt tgcttgtaa aaacctacc agttgtact taacgcgat taaaattta cgcaccaat catcgaggc tgagcaaca aatagaggt gtcagacga agttaaaag aaaagttga aaaaatgtt acaagtgat gcaaacgct taaagaaag cgaggagaa agagaaatc gaagcaaga cgaagcagc gaccactaa cgaacctcc agacgactt ccgccaaat tagtgttta tccaacccc tgccgagat cgtgggagg gcgaggttt cctccggga aaacatcgt caaaggccc ctaccaaag cgtaaacca ctatttaga cgtccagtt tcggctctt acgcgagga tttcgtggg ccctttacg agacggcat aaacaacta tcgatcatc aagaccatc aaacgaaat cgagaacgt gaaaattca caaagacgt ctacttcct gaaaacgga aaccataaa cgagtttta ttgcctccg aactcagtt cagctccaa gaagaaaaa gatcaattt tgagcagtc gaaacgctt catgtttgg gtcgttgct gtgcttcac gaaagacaa ctttcaaac tttgatttt cgggagagt tgcccaaag aaaaatcga ggacttgga taagggcga agtaatcgt cgctttcga cgaaaaaat caaggttga tttcagggc tgcttacct gatggttga atgtagcgt ctacttcga gccgtattt tcacgtcct cactgtttt gaaaggcat gactgagga gatgtttcc gatggagag gtacattat ccgggttca cccgtccgt gcaaccccc tgcatattt aacagcccc caactgcaa gtacaacat tgaagacta tcgatttaa ccctttgtc agagtggcc ccacgagga cttttacgg tttgaatga ctcccagat ggtggcgtt caaagcggc aatcaccca ggaaatcgc cgttattca gggaccccc agggactgg aaaaacgta tcttggact aaagatagc acaaacgca attggaaaa catggactg ttggtacaa tcatacacc catgctggt gatttgttt cacgaacca cgcacttga ccagttttt ggagggttt gcttccatt tacaaaaga gattatacg agttggtgg acggtccca aaacgagaa actggattc atacaattt gagaaacaa cactcagcg agatagcgc tgtttcaga aaaacgttt ccaagtaca gctctgttt aagagaaat aaaacgcat cactgagca tttgaatca gatcgaata ttacaacag ggttttgtc ttttcgtgc ttttcgaga aattccggg tttttctga ttcgtggtt tgtacatgc gtccaatcg agaggtagt tcactggtt actccaggg cgcagatta cgggcaaga tttgtccag tataattga caatttgca agttatgca ggctcagga ggaaatgta cagaattgg catcaacga caatgatga gactcttgg atacgactt taatgaaca tataagtac aattgttga ttcgacgtt tgatgttac tgttgtatc acataaaga tgattacat aattacctt ggactccat gcgcaatta cttgcagca gtacaaaga caacctaca tgacctaca acagataca aaacccatc actccagga tcttatacg agaagaaga gttgcatta ccaaataat ggttttgca aaacaaata cgactacgt caaacacca attgagcga ctggaaaaa catccaagg tgacatcga gccaccaca gaatataga tttgcatga gccctacaa tatgaagtt tgaagacag atggaggct ttattttca ctggctgaa tttgcataa acagcgtga atcagacgt tctgcggat gacttgtga ggattttcc ggcgattta taaggagta tgaagtaat gcgagaaat ggaagacgt gcaagtaat gaaaaaagc gttagtagt gggaatgac gaccaccag tgcagctcg attaagatc gtccttgca aactctgaa aagcccgat tgttattgt agaggaagc ggctgaagt tctggaagc ccacattgt aacctcgat caccaaaca ctgccaaca cttgatttt gatcgggga ccataagca gctgaaacc aaacacggc caattatag cctggaaaa gcagtacca cctgggaat cagtctgtt cgaaagaat gattcgaaa caatattca ctgttacac ccttaacgt gcagcaccg catgcgacc ggaaatttc gtctctgat ccggcccac aatttacga ttttttgga agaccatcc ctcggtgta caacagacc gaaaatatc aggcgtcga caactgcgt atttttcat cgatcatac gcacgctga ggaagagtg cgagggttt gagcaaaac caacttgca cgaagtctc gttttttat ttaccttgc aaggcattt gattttgaa tggttacaa ccctgcaaa catcacgat tctggcggc ttatttggg ccagttttt tgcgttcca gagggagaa gagggagca taaggactt gctgaagga cgtcagggt tgctgtttt ggataatta ccaaggaga ggagtcgga tatcattct tctttcgtt agttcgaaa taacaatga aaataaaat tggttttct aaaaatcga aaaccgtgt atgcgtcgc tctttcgag ggccaggaa tgggttgta tataatggg aaatatgac gcaactgtg ttttgaaaa taaactttg gcgcaaaat caaagcgag tcttgagca acaaaaggc tctcgggac tgaattacc tctcaggtg tcaaattca ccaggacca agtcacgca tgtgaaatc tgagagtga tttcttttc ggtttctga aggcggttg caataaaaa gtgtggcgc ggagctgaa gtgtggtca caactgcaa ctacttgtg ccacattcg ggacaggag ccacatgat ttacaaatg ccaagcaga atgtaaaaa gaatttgtg cgacaaaga cccagcaca tctctgcaa gaaaatgtg ttttgaaga ttgcggtct ttgcgatta cctagtcca cagagtttt agcttgcgg tcacgaagc taacatcca atgccacat tgatccaga aatctacaa atgcgaaat tttggtttc aaccgaatt gccttgtgg ccacatggc tgacaaacc ctgcttttc caatcctgt aactttcag gtgtccaca catttgcga tgttcaagt acagccttg tggtcatgc atgcgaatt attatgcca catccgtaa agatcctga ccatttaga atataagtg tcagaaacc ttgcacccg atttatgaa atattgcac cgtcaatga tgaggatca tagatgcag gaagaagtg tttcgaaga ctgcgacgt ttgcacagt tgttgtaag aaagaagag aactgtgtg caaccacta ttacgacgt ccgttgctg tgaagatgt ggacgacat cgactgtga aaaaccttg tgctaagat gcgaaactg tggccaccc ttgcaaacg caagtgcaa tgaaccttg ccaagagtg ccaaatcaa ggtgactaa aatccaccc catctgcaa ccatcccgt gaagatgaa atgctgcga gaagcctga tcgcaagta ctgcaacaa aaactgtcc tttgacact gccttgtgg ccacccttg caccaaaaa gtgtaacga gccctgcac ccccgcctg tggtgtcct ggtggactg ttcgggcac cgaagcgga atgtggcca cctggtgac cagaatgga gtgccacat ggccacatc ttccgtcga ccccaaacg tctaataca cttctgcaa cgctccctg caaacaaga gctaaaatg tggccattt gtgctcggg ctcctgcgg tgagtgcca ccaaggccg catccacaa gcgatgctc gcaaaaatg tggggcccc gttggtgtg cgaccaccc ttgcgatat cccctgcag acaggcctg cagaccctg cagacgggt ctgttccta cacttgtgg gcacagcat ttgcaaaaa gaagtgcgg cgaagtctg cacgtcttg caaagagcc gtgtttgag gcggtgccc ccaccagtg gtgcactaa gctgtgtgg ggagcgctg cagtgtgcc cccatgcac gcaaccgtg ccagaaggc gctcaaatg tgggcactt gtgtattgg tttttgtgg ggatccgtg tccgccaaa gtgtttggt gtgcgacaa ggaggcgtt gcttgaagt gttctttgg aacggaaag tgaagacgc cagatatgt tctactgaa cgactgcaa acacattgt tgaaaatga gggcatgga gcactggct caaacaaaa cgagtttca gattagtta caaattgtg tccaatgtg caaaactgc gattaaaac cactcagcg gtaccggga ttacatcaa aatggccat caaagatgt ggctcaagt gaagatcaa agcgaacgg gagtcctgc cgaaatcag ggaaaaaac tgacaaaat caaagaaac tttacaagc cttggaaac caaatgtag attacttaa aatgtactg cggacccat cacagacgt gttgaggtc cctcaagtc acgaattca acagtccaa caagggcaa gagacaaca cttgaacat tttcgatgt ccagtccct tatggcgaa gctgcaaat agtagtcca cattgctaa catttgctg caacgagaa agtaattgt ggaaacact gcatgaaat ttttttccc gcaagtgtc cctcattgt cgaaattct gatgcgaga cgtggacat aacaaatca agaaacaag cgatttgag ttacgaaat caacagact gtcgcgaat tgtggattt cagctgcct tcaaaaaac cccacaatt ttcctatca taaagccaa caatgcgac tgccaaatc attgataag cactgtgga aaataagct tttttcttg ccagaaatt tacaaacga gaccaacga tttgttgaa ggattgttt aagggaatt gaacgatgt tatgaaatc gggactggc tatttcgga tcaggaaag aagagaaat tttgcaggc tatgggttt tagtaaagg gcactggta caagtgtcc caatgggca cgtctatgc tattggtga atgtggggg cgctatgca ggaagcaac gtgcaatga gtgcggggc tcgaattgg aggctccca gcatagact tctggagaa taatgctgt ggccactga aatggatgg ggctgttca tggggcttg gaccatcgc aactaattt tcataatta tgctctatg ataaactaa gtggaattt taattacat gtattttat cagtaatcc aaaactttt tttttacaa tgtatctgc taaatacta aaaaatgat gtgttcgta atgtaattt gttctaata aagaaattt aagcaat
