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Gene information
General information
TC002932 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 1495839..1498751
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002932
    iBeetle screen iB_03471
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002932 RA
      MNCEDQVIQ PINRDTVHR ICSGQVVLS LAIAVKELV 
      ENAIDAGAT IIDIQLKEY GSEIIEVSD NGSGVLKEN 
      FQALTLKHY TSKIKQFDD LENLSTLGF RGEALSSLC 
      ALSDLSIVT KHTSAENAT KITYDRSGK IISETVSAR 
      ESGTTVTLE NLFSTLPVR RREFTKNLK REFNKMCQL 
      LYAYCLVSK GIKFSCTNT TSKGSKNTV VATEGLNMV 
      KDNITNVFG PKQLPSLIE VEMVRPDET ILAEYGIKL 
      TEGDPLPFT FEFFISSVM HGSGRSTND RQFFYINSR 
      PCDPSKVAR LVNEIYKQY NNNQYPFVY LNITSKSSL 
      VDVNVTPDK RQVFLEKEK VLLSTIKAS LIEAFKMFP 
      STYKMQNLD LTQNTTIKD FFKDSDCSP RGLKRSVTE 
      SELKKGSTL ERFKKRSKT ENDKDLLAS PNHSSSLKE 
      KRKVDNNSQ LDTFIKIAS EIIARDKRE EKSFNALCE 
      EEDIEETKT VCTNTFIKT TSQIIAKDD CGTNKSEEK 
      SLNTLCEED IIEEIKTVC SSTTKEAEN IVTIPKTIN 
      ECEDKEEIE KTNEIKITK SPSQLDNRI EEKKEKKDS 
      GEEPTKNES KFEKSVAED KLLQDAPLK SRESRKTVN 
      LDVSLEDIR KSIEQKVTK DNDIKVRFR SKINPDSNK 
      SAEEELRKH ISKEDFANM DVIGQFNLG FIITKLKND 
      LFIIDQHAT DEKYNFEQL QASTVMENQ VLVNPKPLQ 
      LTAGNESLL IENEDIFNK NGFTFKIDE SAPCTQKVS 
      LTSIPLSKS MVFGKQDIE EMLFMLQDS NHTMCRPSR 
      IRAMFATRA CRKSVMIGK PLSKSDMRR LVNHMGEIE 
      QPWNCPHGR PTMRHLINL DLIQEET
    CDS sequence
    • >TC002932 RA
      atgaattgt gaggaccaa gtgatacaa ccaataaat 
      cgcgataca gttcaccga atatgttct ggtcaagta 
      gtcttaagt ttagcaatc gctgtgaaa gaactggtc 
      gagaacgcc attgacgcg ggggccaca atcatcgac 
      attcagctt aaagaatac ggctctgaa atcattgaa 
      gtttcagac aacgggagt ggagttcta aaagagaac 
      tttcaagct ctcactctg aaacactac acttcaaag 
      ataaaacag tttgatgat ctggaaaac ctctcaact 
      ttggggttt aggggcgag gcccttagc agtttatgc 
      gctttgtct gatcttagc attgtaaca aaacacaca 
      agtgcggaa aacgcgact aaaattacc tacgatagg 
      agtggcaaa attatcagt gagacggta agtgcgcgg 
      gaaagtggc acaacagtc actttggaa aatctgttt 
      tcaacactg ccagtgagg cgccgagaa ttcactaaa 
      aacttgaaa cgagaattt aataaaatg tgccagcta 
      ctatatgct tactgtttg gtatcaaaa gggattaag 
      ttttcgtgt acaaatact acgagtaaa ggtagcaag 
      aacacggta gtggccaca gagggctta aacatggtc 
      aaagacaac ataactaac gtttttggg cctaaacaa 
      ctcccttct ttaattgag gttgaaatg gttagacct 
      gacgagacg atattggct gaatatgga atcaaacta 
      actgaagga gatcccttg ccttttact tttgagttc 
      tttatatca agtgtcatg catggtagt ggacgcagt 
      accaatgat agacagttt ttttacata aactcacgg 
      ccctgtgat ccctccaaa gtcgcaaga ttagtaaat 
      gaaatttat aaacaatac aataacaat cagtatccg 
      ttcgtttac ctaaatatc acaagcaag tcgtcttta 
      gttgatgta aatgtaact ccagataag cgacaagta 
      tttttagaa aaagaaaaa gttttgtta agcacaatt 
      aaagcctct ttgattgaa gcttttaaa atgtttcct 
      tccacgtat aaaatgcaa aatttggac cttacacaa 
      aacacgaca atcaaagat ttttttaaa gacagtgat 
      tgttcaccg agaggttta aaaagaagt gtcactgag 
      agtgaactt aaaaaaggt agcactttg gagagattc 
      aaaaaaagg tctaaaacc gaaaacgat aaagacttg 
      ttggcaagt cccaatcat agttctagc ttgaaagaa 
      aaaagaaaa gttgataat aattcccag ctggatact 
      tttattaaa atagcttca gaaattatt gctagagat 
      aaacgagaa gagaaatca tttaatgcc ctttgtgaa 
      gaagaggat attgaagaa acaaagaca gtatgtacc 
      aatactttt attaaaaca acttcacaa attattgct 
      aaagatgat tgtggtaca aataaatca gaggagaaa 
      tcccttaat accctttgt gaagaagat attattgaa 
      gaaataaag acagtatgt agcagtact accaaggaa 
      gcggaaaat atcgtcaca attcctaaa accatcaat 
      gaatgtgaa gataaagaa gaaatcgaa aaaacaaat 
      gaaattaaa attacgaaa tcaccttca cagctagat 
      aatagaata gaagaaaag aaagaaaag aaagattct 
      ggagaagag ccaacaaaa aacgaatca aagttcgaa 
      aaaagtgta gccgaggat aaattatta caagatgcg 
      ccactgaaa agtcgtgag tctcgaaaa acagtaaat 
      ttagatgtt tccttagaa gacattaga aaatcaata 
      gaacaaaaa gtcacaaaa gacaatgat atcaaagtg 
      cgttttcgt tcgaaaatc aatccagac tcaaataaa 
      tctgcagaa gaagagttg cgaaaacac atctctaag 
      gaagacttt gctaacatg gacgtaatc ggtcagttt 
      aatttaggt tttattatc actaaatta aaaaacgat 
      ttatttatc attgaccaa cacgcaacc gacgaaaaa 
      tataatttc gaacaactg caagctagt acagtcatg 
      gaaaaccaa gtccttgtc aatccaaag cctctccag 
      ttaacggca ggaaacgaa agtctgctc atcgaaaat 
      gaggacatt ttcaataaa aacggcttc acgttcaaa 
      atcgacgag tcggccccg tgtactcaa aaagtgtct 
      ctaacttca ataccacta agcaagagt atggtattt 
      ggtaagcag gacattgag gagatgctc tttatgtta 
      caagattcg aatcacaca atgtgtagg ccatctaga 
      attcgggca atgtttgca actagggct tgtcgcaag 
      tccgtcatg attggaaaa cctttatca aagagcgat 
      atgaggagg ctagttaac catatgggc gaaatagaa 
      caaccttgg aattgtcca cacggacga ccaacaatg 
      cgtcattta attaattta gacttgata caagaagaa 
      acgtag
    mRNA sequence
    • >TC002932 RA
      aaatcacga tgaattgtg aggaccaag tgatacaac 
      caataaatc gcgatacag ttcaccgaa tatgttctg 
      gtcaagtag tcttaagtt tagcaatcg ctgtgaaag 
      aactggtcg agaacgcca ttgacgcgg gggccacaa 
      tcatcgaca ttcagctta aagaatacg gctctgaaa 
      tcattgaag tttcagaca acgggagtg gagttctaa 
      aagagaact ttcaagctc tcactctga aacactaca 
      cttcaaaga taaaacagt ttgatgatc tggaaaacc 
      tctcaactt tggggttta ggggcgagg cccttagca 
      gtttatgcg ctttgtctg atcttagca ttgtaacaa 
      aacacacaa gtgcggaaa acgcgacta aaattacct 
      acgatagga gtggcaaaa ttatcagtg agacggtaa 
      gtgcgcggg aaagtggca caacagtca ctttggaaa 
      atctgtttt caacactgc cagtgaggc gccgagaat 
      tcactaaaa acttgaaac gagaattta ataaaatgt 
      gccagctac tatatgctt actgtttgg tatcaaaag 
      ggattaagt tttcgtgta caaatacta cgagtaaag 
      gtagcaaga acacggtag tggccacag agggcttaa 
      acatggtca aagacaaca taactaacg tttttgggc 
      ctaaacaac tcccttctt taattgagg ttgaaatgg 
      ttagacctg acgagacga tattggctg aatatggaa 
      tcaaactaa ctgaaggag atcccttgc cttttactt 
      ttgagttct ttatatcaa gtgtcatgc atggtagtg 
      gacgcagta ccaatgata gacagtttt tttacataa 
      actcacggc cctgtgatc cctccaaag tcgcaagat 
      tagtaaatg aaatttata aacaataca ataacaatc 
      agtatccgt tcgtttacc taaatatca caagcaagt 
      cgtctttag ttgatgtaa atgtaactc cagataagc 
      gacaagtat ttttagaaa aagaaaaag ttttgttaa 
      gcacaatta aagcctctt tgattgaag cttttaaaa 
      tgtttcctt ccacgtata aaatgcaaa atttggacc 
      ttacacaaa acacgacaa tcaaagatt tttttaaag 
      acagtgatt gttcaccga gaggtttaa aaagaagtg 
      tcactgaga gtgaactta aaaaaggta gcactttgg 
      agagattca aaaaaaggt ctaaaaccg aaaacgata 
      aagacttgt tggcaagtc ccaatcata gttctagct 
      tgaaagaaa aaagaaaag ttgataata attcccagc 
      tggatactt ttattaaaa tagcttcag aaattattg 
      ctagagata aacgagaag agaaatcat ttaatgccc 
      tttgtgaag aagaggata ttgaagaaa caaagacag 
      tatgtacca atactttta ttaaaacaa cttcacaaa 
      ttattgcta aagatgatt gtggtacaa ataaatcag 
      aggagaaat cccttaata ccctttgtg aagaagata 
      ttattgaag aaataaaga cagtatgta gcagtacta 
      ccaaggaag cggaaaata tcgtcacaa ttcctaaaa 
      ccatcaatg aatgtgaag ataaagaag aaatcgaaa 
      aaacaaatg aaattaaaa ttacgaaat caccttcac 
      agctagata atagaatag aagaaaaga aagaaaaga 
      aagattctg gagaagagc caacaaaaa acgaatcaa 
      agttcgaaa aaagtgtag ccgaggata aattattac 
      aagatgcgc cactgaaaa gtcgtgagt ctcgaaaaa 
      cagtaaatt tagatgttt ccttagaag acattagaa 
      aatcaatag aacaaaaag tcacaaaag acaatgata 
      tcaaagtgc gttttcgtt cgaaaatca atccagact 
      caaataaat ctgcagaag aagagttgc gaaaacaca 
      tctctaagg aagactttg ctaacatgg acgtaatcg 
      gtcagttta atttaggtt ttattatca ctaaattaa 
      aaaacgatt tatttatca ttgaccaac acgcaaccg 
      acgaaaaat ataatttcg aacaactgc aagctagta 
      cagtcatgg aaaaccaag tccttgtca atccaaagc 
      ctctccagt taacggcag gaaacgaaa gtctgctca 
      tcgaaaatg aggacattt tcaataaaa acggcttca 
      cgttcaaaa tcgacgagt cggccccgt gtactcaaa 
      aagtgtctc taacttcaa taccactaa gcaagagta 
      tggtatttg gtaagcagg acattgagg agatgctct 
      ttatgttac aagattcga atcacacaa tgtgtaggc 
      catctagaa ttcgggcaa tgtttgcaa ctagggctt 
      gtcgcaagt ccgtcatga ttggaaaac ctttatcaa 
      agagcgata tgaggaggc tagttaacc atatgggcg 
      aaatagaac aaccttgga attgtccac acggacgac 
      caacaatgc gtcatttaa ttaatttag acttgatac 
      aagaagaaa cgtagatga tttcacaaa tcattttag 
      acttgatta ttttcatta aattgtcca catggcc