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Gene information
General information
TC002914 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 1783650..1822035
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002914
    iBeetle screen iB_03469
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not completely molted - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    Pupal morphology
    oblique dorsal thoracic muscles median broader - Number of animals showing the phenotype: 5

    Comment: muscle potentially split/not fused or ectopic muscle present
    oblique dorsal thoracic muscles median shortened - Number of animals showing the phenotype: 5
    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002914 RA
      MASSHKFLL LLLRMAGLW LVNAAGALR EQKFAMEPQ 
      DQTAVEGSR VTLPCRVID KSGILQWTK DDFGLGTHR 
      NLSGFDRYS MIGSDEEGD YSLDIYPVT LDDDAKYQC 
      QVSPGPQGQ PGIRSHYAT VTVLVPPDP PKIVQGDYL 
      VTTEDREIE LECISHGGK PAAEITWID GLGNVLTDG 
      IEYIVEELS DGRRFTAKS ILKLTPRKD HHNTTFTCQ 
      AQNTAERTH RSAKLKLEV KYAPKVTVS ILAGATQSG 
      RIPEGAEVR LGCHADANP ADVTYRWFL NDEPLIGGY 
      TTEMVIHNA TRKYHDAIV KCEVHNAVG KSEESETLD 
      ISYGPQFRT KPRSVQADL GTSVTLTCD VDGNPLPEI 
      SWFDEKHKR IIHNSPNLT LRVDQETAG RYFCKAHVP 
      GFPEIGADA TIYLKGPPS IVSHRTQFG IEGDNVRVE 
      CTIFSIPLP ERIVWTFNG REIDIHDQD YTILEDPSP 
      EGIKSTLVI RESQEKHFG MYNCSVTNP YGSDVVEIN 
      LMQQKNVPS IIIIFGVAA LVILVFIVI LVVFLCQKQ 
      SKKKPPPET DANTIEKQC KESDRSSNI SDLKLDLRT 
      GSSNSNVHC DMDYIPGAE SETGSESVI TRIGVPLAG 
      PVNVSGQEI YRYSADYTE PSFPPKDGQ NNNGYVPYV 
      DYSRDYNPP MMQPPISSS LDPGRESLQ STRLQLNSL 
      QAHQVSGQN LPLNDLSDL HLAQGVPNG GLPGLGPID 
      PRFSATYGN PYLRNPNVG LPTPNTANP AATPAPPPY 
      SRDSSRIAS SSAMMTASA NVANTVVAN GANGSVPQV 
      TRLPNSPTS QYIVPNSNM ATIKRGTMA THV
    • >TC002914 RB
      MASSHKFLL LLLRMAGLW LVNAAGALR EQKFAMEPQ 
      DQTAVEGSR VTLPCRVID KSGILQWTK DDFGLGTHR 
      NLSGFDRYS MIGSDEEGD YSLDIYPVT LDDDAKYQC 
      QVSPGPQGQ PGIRSHYAT VTVLVPPDP PKIVQGDYL 
      VTTEDREIE LECISHGGK PAAEITWID GLGNVLTDG 
      IEYIVEELS DGRRFTAKS ILKLTPRKD HHNTTFTCQ 
      AQNTAERTH RSAKLKLEV KYAPKVTVS ILAGATQSG 
      RIPEGAEVR LGCHADANP ADVTYRWFL NDEPLIGGY 
      TTEMVIHNA TRKYHDAIV KCEVHNAVG KSEESETLD 
      ISYGPQFRT KPRSVQADL GTSVTLTCD VDGNPLPEI 
      SWFDEKHKR IIHNSPNLT LRVDQETAG RYFCKAHVP 
      GFPEIGADA TIYLKGPPS IVSHRTQFG IEGDNVRVE 
      CTIFSIPLP ERIVWTFNG REIDIHDQD YTILEDPSP 
      EGIKSTLVI RESQEKHFG MYNCSVTNP YGSDVVEIN 
      LMQQKNVPS IIIIFGVAA LVILVFIVI LVVFLCQKQ 
      SKKKPPPET DANTIEKQC KESDRSSNI SDLKLDLRT 
      GSSNSNVHC DMDYIPGAE SETGRQEIY RYSADYTEP 
      SFPPKDGQN NNGYVPYVD YSRDYNPPM MQPPISSSL 
      DPGRESLQS TRLQLNSLQ AHQVSGQNL PLNDLSDLH 
      LAQGVPNGG LPGLGPIDP RFSATYGNP YLRNPNVGL 
      PTPNTANPA ATPAPPPYS RDSSRIASS SAMMTASAN 
      VANTVVANG ANGSVPQVT RLPNSPTSQ YIVPNSNMA 
      TIKRGTMAT HV
    • >TC002914 RC
      MASSHKFLL LLLRMAGLW LVNAAGALR EQKFAMEPQ 
      DQTAVEGSR VTLPCRVID KSGILQWTK DDFGLGTHR 
      NLSGFDRYS MIGSDEEGD YSLDIYPVT LDDDAKYQC 
      QVSPGPQGQ PGIRSHYAT VTVLVPPDP PKIVQGDYL 
      VTTEDREIE LECISHGGK PAAEITWID GLGNVLTDG 
      IEYIVEELS DGRRFTAKS ILKLTPRKD HHNTTFTCQ 
      AQNTAERTH RSAKLKLEV KYAPKVTVS ILAGATQSG 
      RIPEGAEVR LGCHADANP ADVTYRWFL NDEPLIGGY 
      TTEMVIHNA TRKYHDAIV KCEVHNAVG KSEESETLD 
      ISYGPQFRT KPRSVQADL GTSVTLTCD VDGNPLPEI 
      SWFDEKHKR IIHNSPNLT LRVDQETAG RYFCKAHVP 
      GFPEIGADA TIYLKGPPS IVSHRTQFG IEGDNVRVE 
      CTIFSIPLP ERIVWTFNG REIDIHDQD YTILEDPSP 
      EGIKSTLVI RESQEKHFG MYNCSVTNP YGSDVVEIN 
      LMQQKNVPS IIIIFGVAA LVILVFIVI LVVFLCQKQ 
      SKKKPPPEK KQCKESDRS SNISDLKLD LRTGSSNSN 
      VHCDMDYIP GAESETGSE SVITRIGVP LAGPVNVSG 
      QEIYRYSAD YTEPSFPPK DGQNNNGYV PYVDYSRDY 
      NPPMMQPPI SSSLDPGRE SLQSTRLQL NSLQAHQVS 
      GQNLPLNDL SDLHLAQGV PNGGLPGLG PIDPRFSAT 
      YGNPYLRNP NVGLPTPNT ANPAATPAP PPYSRDSSR 
      IASSSAMMT ASANVANTV VANGANGSV PQVTRLPNS 
      PTSQYIVPN SNMATIKRG TMATHV
    CDS sequence
    • >TC002914 RA
      atggcatca tcgcacaag ttcctgctg ctcctcctg 
      cggatggcc ggtctgtgg cttgtgaat gcagcagga 
      gcgctccgc gaacaaaag ttcgcaatg gagccccaa 
      gaccagacg gccgtggaa gggtccagg gtgacgctg 
      ccctgcaga gttatcgac aagagcggc atcctgcag 
      tggaccaag gacgacttc ggcctgggc acgcacagg 
      aatctcagc ggattcgat cgctactcc atgattggc 
      agcgatgaa gaaggcgac tattccctg gacatctac 
      cccgtgacg ttggacgac gacgccaaa taccaatgc 
      caagtgtct cccggtccc cagggccag ccgggaatt 
      cgcagccat tacgcgacg gtgactgtg ctcgtgccc 
      cccgatccg cccaagatc gtgcaaggg gattacctg 
      gtcacgacg gaagacagg gaaatagaa ctggagtgc 
      ataagtcac ggcgggaag ccggcggcg gaaataacc 
      tggattgat ggcctggga aacgtccta acagacgga 
      attgaatat atcgtcgag gagctatca gacggcagg 
      agattcacg gccaagtca atactcaaa ctgacgccc 
      agaaaggac caccacaac accaccttc acctgccag 
      gcccagaac acggccgag cggacgcac cggtccgcc 
      aaactcaag ctggaggta aaatatgcg ccgaaagtc 
      acggtctct atcctggcg ggagccacg cagagcgga 
      aggattcct gagggggcg gaggtcaga ctggggtgt 
      catgcggat gctaaccct gcagacgtc acctacagg 
      tggttcttg aacgatgaa ccgttaatt gggggatac 
      accaccgag atggttatt cacaacgcg accaggaaa 
      tatcacgac gctattgtc aaatgcgaa gtgcacaat 
      gcagttggc aagagcgag gagagcgaa acgctggat 
      attagttac ggtccccag tttcgcaca aagccgcgt 
      tcagtgcag gccgacctg ggcacaagc gtaactctg 
      acctgcgac gtggacggt aatcccctt ccggagata 
      tcgtggttc gacgaaaag cacaaacga atcatccac 
      aacagcccc aatttaacg ttgcgcgtc gatcaagag 
      acggcggga agatatttt tgcaaagcc cacgttccc 
      ggatttcct gaaatcggt gcggatgca acaatttac 
      ctcaaaggc cctccgtct atagttagc caccgcact 
      cagttcggc atcgagggc gataatgtt cgcgtcgag 
      tgcacgata ttttccata ccgctgcca gagcgaatc 
      gtctggaca tttaacgga cgagagatc gacattcac 
      gaccaagat tatacgata ttggaagat ccctctcct 
      gagggcatc aaatctaca ttggtaata agggaatct 
      caggagaaa cattttggc atgtataat tgcagcgtc 
      acgaatccg tacggaagc gacgtcgtt gaaattaat 
      ctgatgcaa caaaaaaac gtcccgtca atcatcatc 
      attttcggc gtcgctgcg ctggttata ctcgtcttt 
      attgtgatt ttggtcgtt ttcctgtgc cagaaacag 
      agcaagaag aagccgcca ccagaaact gatgccaat 
      acaatagaa aaacaatgc aaagaatct gacagaagt 
      tccaatatc tccgacttg aaactggat ctgaggacg 
      ggctcttca aacagcaat gtgcactgt gacatggat 
      tatattccc ggggcggag tccgaaaca ggaagtgag 
      tctgttata acgcgaatc ggtgtccct ttagctggc 
      cccgtcaat gtttcaggg caagaaatc taccgctac 
      tccgccgat tacaccgaa ccaagcttc ccgcctaaa 
      gacggccag aacaacaac ggctacgtg ccatacgtg 
      gactactcg cgggactac aaccccccg atgatgcag 
      ccccccatc tcgtcctcg ctggacccc gggcgcgag 
      agcctgcag tccacgcgc ctgcagctc aactcgctg 
      caggcgcac caggtgtcc gggcagaac ctgccgttg 
      aacgacctc tccgacctg cacctggcg cagggcgtg 
      cccaacggg gggctgccg gggctgggg cccatcgac 
      ccgcgcttc agcgccacc tacggcaac ccctacctg 
      cgcaacccc aacgtgggg ctgcccacc cccaacacc 
      gccaacccg gcggccacg cccgcgccg cccccctac 
      tcgcgggac agctcccgg atagctagt tctagtgct 
      atgatgact gccagtgcg aacgtggcg aacacggtg 
      gtggcgaac ggtgccaac gggtcggtg ccccaggtg 
      acgcggctt ccgaactcc cccacgagt caatacata 
      gttcccaat tcgaacatg gcaacgatc aagagaggt 
      actatggcc acgcacgta tag
    • >TC002914 RB
      atggcatca tcgcacaag ttcctgctg ctcctcctg 
      cggatggcc ggtctgtgg cttgtgaat gcagcagga 
      gcgctccgc gaacaaaag ttcgcaatg gagccccaa 
      gaccagacg gccgtggaa gggtccagg gtgacgctg 
      ccctgcaga gttatcgac aagagcggc atcctgcag 
      tggaccaag gacgacttc ggcctgggc acgcacagg 
      aatctcagc ggattcgat cgctactcc atgattggc 
      agcgatgaa gaaggcgac tattccctg gacatctac 
      cccgtgacg ttggacgac gacgccaaa taccaatgc 
      caagtgtct cccggtccc cagggccag ccgggaatt 
      cgcagccat tacgcgacg gtgactgtg ctcgtgccc 
      cccgatccg cccaagatc gtgcaaggg gattacctg 
      gtcacgacg gaagacagg gaaatagaa ctggagtgc 
      ataagtcac ggcgggaag ccggcggcg gaaataacc 
      tggattgat ggcctggga aacgtccta acagacgga 
      attgaatat atcgtcgag gagctatca gacggcagg 
      agattcacg gccaagtca atactcaaa ctgacgccc 
      agaaaggac caccacaac accaccttc acctgccag 
      gcccagaac acggccgag cggacgcac cggtccgcc 
      aaactcaag ctggaggta aaatatgcg ccgaaagtc 
      acggtctct atcctggcg ggagccacg cagagcgga 
      aggattcct gagggggcg gaggtcaga ctggggtgt 
      catgcggat gctaaccct gcagacgtc acctacagg 
      tggttcttg aacgatgaa ccgttaatt gggggatac 
      accaccgag atggttatt cacaacgcg accaggaaa 
      tatcacgac gctattgtc aaatgcgaa gtgcacaat 
      gcagttggc aagagcgag gagagcgaa acgctggat 
      attagttac ggtccccag tttcgcaca aagccgcgt 
      tcagtgcag gccgacctg ggcacaagc gtaactctg 
      acctgcgac gtggacggt aatcccctt ccggagata 
      tcgtggttc gacgaaaag cacaaacga atcatccac 
      aacagcccc aatttaacg ttgcgcgtc gatcaagag 
      acggcggga agatatttt tgcaaagcc cacgttccc 
      ggatttcct gaaatcggt gcggatgca acaatttac 
      ctcaaaggc cctccgtct atagttagc caccgcact 
      cagttcggc atcgagggc gataatgtt cgcgtcgag 
      tgcacgata ttttccata ccgctgcca gagcgaatc 
      gtctggaca tttaacgga cgagagatc gacattcac 
      gaccaagat tatacgata ttggaagat ccctctcct 
      gagggcatc aaatctaca ttggtaata agggaatct 
      caggagaaa cattttggc atgtataat tgcagcgtc 
      acgaatccg tacggaagc gacgtcgtt gaaattaat 
      ctgatgcaa caaaaaaac gtcccgtca atcatcatc 
      attttcggc gtcgctgcg ctggttata ctcgtcttt 
      attgtgatt ttggtcgtt ttcctgtgc cagaaacag 
      agcaagaag aagccgcca ccagaaact gatgccaat 
      acaatagaa aaacaatgc aaagaatct gacagaagt 
      tccaatatc tccgacttg aaactggat ctgaggacg 
      ggctcttca aacagcaat gtgcactgt gacatggat 
      tatattccc ggggcggag tccgaaaca ggaaggcaa 
      gaaatctac cgctactcc gccgattac accgaacca 
      agcttcccg cctaaagac ggccagaac aacaacggc 
      tacgtgcca tacgtggac tactcgcgg gactacaac 
      cccccgatg atgcagccc cccatctcg tcctcgctg 
      gaccccggg cgcgagagc ctgcagtcc acgcgcctg 
      cagctcaac tcgctgcag gcgcaccag gtgtccggg 
      cagaacctg ccgttgaac gacctctcc gacctgcac 
      ctggcgcag ggcgtgccc aacgggggg ctgccgggg 
      ctggggccc atcgacccg cgcttcagc gccacctac 
      ggcaacccc tacctgcgc aaccccaac gtggggctg 
      cccaccccc aacaccgcc aacccggcg gccacgccc 
      gcgccgccc ccctactcg cgggacagc tcccggata 
      gctagttct agtgctatg atgactgcc agtgcgaac 
      gtggcgaac acggtggtg gcgaacggt gccaacggg 
      tcggtgccc caggtgacg cggcttccg aactccccc 
      acgagtcaa tacatagtt cccaattcg aacatggca 
      acgatcaag agaggtact atggccacg cacgtatag
    • >TC002914 RC
      atggcatca tcgcacaag ttcctgctg ctcctcctg 
      cggatggcc ggtctgtgg cttgtgaat gcagcagga 
      gcgctccgc gaacaaaag ttcgcaatg gagccccaa 
      gaccagacg gccgtggaa gggtccagg gtgacgctg 
      ccctgcaga gttatcgac aagagcggc atcctgcag 
      tggaccaag gacgacttc ggcctgggc acgcacagg 
      aatctcagc ggattcgat cgctactcc atgattggc 
      agcgatgaa gaaggcgac tattccctg gacatctac 
      cccgtgacg ttggacgac gacgccaaa taccaatgc 
      caagtgtct cccggtccc cagggccag ccgggaatt 
      cgcagccat tacgcgacg gtgactgtg ctcgtgccc 
      cccgatccg cccaagatc gtgcaaggg gattacctg 
      gtcacgacg gaagacagg gaaatagaa ctggagtgc 
      ataagtcac ggcgggaag ccggcggcg gaaataacc 
      tggattgat ggcctggga aacgtccta acagacgga 
      attgaatat atcgtcgag gagctatca gacggcagg 
      agattcacg gccaagtca atactcaaa ctgacgccc 
      agaaaggac caccacaac accaccttc acctgccag 
      gcccagaac acggccgag cggacgcac cggtccgcc 
      aaactcaag ctggaggta aaatatgcg ccgaaagtc 
      acggtctct atcctggcg ggagccacg cagagcgga 
      aggattcct gagggggcg gaggtcaga ctggggtgt 
      catgcggat gctaaccct gcagacgtc acctacagg 
      tggttcttg aacgatgaa ccgttaatt gggggatac 
      accaccgag atggttatt cacaacgcg accaggaaa 
      tatcacgac gctattgtc aaatgcgaa gtgcacaat 
      gcagttggc aagagcgag gagagcgaa acgctggat 
      attagttac ggtccccag tttcgcaca aagccgcgt 
      tcagtgcag gccgacctg ggcacaagc gtaactctg 
      acctgcgac gtggacggt aatcccctt ccggagata 
      tcgtggttc gacgaaaag cacaaacga atcatccac 
      aacagcccc aatttaacg ttgcgcgtc gatcaagag 
      acggcggga agatatttt tgcaaagcc cacgttccc 
      ggatttcct gaaatcggt gcggatgca acaatttac 
      ctcaaaggc cctccgtct atagttagc caccgcact 
      cagttcggc atcgagggc gataatgtt cgcgtcgag 
      tgcacgata ttttccata ccgctgcca gagcgaatc 
      gtctggaca tttaacgga cgagagatc gacattcac 
      gaccaagat tatacgata ttggaagat ccctctcct 
      gagggcatc aaatctaca ttggtaata agggaatct 
      caggagaaa cattttggc atgtataat tgcagcgtc 
      acgaatccg tacggaagc gacgtcgtt gaaattaat 
      ctgatgcaa caaaaaaac gtcccgtca atcatcatc 
      attttcggc gtcgctgcg ctggttata ctcgtcttt 
      attgtgatt ttggtcgtt ttcctgtgc cagaaacag 
      agcaagaag aagccgcca ccagaaaaa aaacaatgc 
      aaagaatct gacagaagt tccaatatc tccgacttg 
      aaactggat ctgaggacg ggctcttca aacagcaat 
      gtgcactgt gacatggat tatattccc ggggcggag 
      tccgaaaca ggaagtgag tctgttata acgcgaatc 
      ggtgtccct ttagctggc cccgtcaat gtttcaggg 
      caagaaatc taccgctac tccgccgat tacaccgaa 
      ccaagcttc ccgcctaaa gacggccag aacaacaac 
      ggctacgtg ccatacgtg gactactcg cgggactac 
      aaccccccg atgatgcag ccccccatc tcgtcctcg 
      ctggacccc gggcgcgag agcctgcag tccacgcgc 
      ctgcagctc aactcgctg caggcgcac caggtgtcc 
      gggcagaac ctgccgttg aacgacctc tccgacctg 
      cacctggcg cagggcgtg cccaacggg gggctgccg 
      gggctgggg cccatcgac ccgcgcttc agcgccacc 
      tacggcaac ccctacctg cgcaacccc aacgtgggg 
      ctgcccacc cccaacacc gccaacccg gcggccacg 
      cccgcgccg cccccctac tcgcgggac agctcccgg 
      atagctagt tctagtgct atgatgact gccagtgcg 
      aacgtggcg aacacggtg gtggcgaac ggtgccaac 
      gggtcggtg ccccaggtg acgcggctt ccgaactcc 
      cccacgagt caatacata gttcccaat tcgaacatg 
      gcaacgatc aagagaggt actatggcc acgcacgta 
      tag
    mRNA sequence
    • >TC002914 RA
      cgccgcccg tctgagatg ctttgaagc agagcaatt 
      atttagaga taaaataag acaatcaag atggcatca 
      tcgcacaag ttcctgctg ctcctcctg cggatggcc 
      ggtctgtgg cttgtgaat gcagcagga gcgctccgc 
      gaacaaaag ttcgcaatg gagccccaa gaccagacg 
      gccgtggaa gggtccagg gtgacgctg ccctgcaga 
      gttatcgac aagagcggc atcctgcag tggaccaag 
      gacgacttc ggcctgggc acgcacagg aatctcagc 
      ggattcgat cgctactcc atgattggc agcgatgaa 
      gaaggcgac tattccctg gacatctac cccgtgacg 
      ttggacgac gacgccaaa taccaatgc caagtgtct 
      cccggtccc cagggccag ccgggaatt cgcagccat 
      tacgcgacg gtgactgtg ctcgtgccc cccgatccg 
      cccaagatc gtgcaaggg gattacctg gtcacgacg 
      gaagacagg gaaatagaa ctggagtgc ataagtcac 
      ggcgggaag ccggcggcg gaaataacc tggattgat 
      ggcctggga aacgtccta acagacgga attgaatat 
      atcgtcgag gagctatca gacggcagg agattcacg 
      gccaagtca atactcaaa ctgacgccc agaaaggac 
      caccacaac accaccttc acctgccag gcccagaac 
      acggccgag cggacgcac cggtccgcc aaactcaag 
      ctggaggta aaatatgcg ccgaaagtc acggtctct 
      atcctggcg ggagccacg cagagcgga aggattcct 
      gagggggcg gaggtcaga ctggggtgt catgcggat 
      gctaaccct gcagacgtc acctacagg tggttcttg 
      aacgatgaa ccgttaatt gggggatac accaccgag 
      atggttatt cacaacgcg accaggaaa tatcacgac 
      gctattgtc aaatgcgaa gtgcacaat gcagttggc 
      aagagcgag gagagcgaa acgctggat attagttac 
      ggtccccag tttcgcaca aagccgcgt tcagtgcag 
      gccgacctg ggcacaagc gtaactctg acctgcgac 
      gtggacggt aatcccctt ccggagata tcgtggttc 
      gacgaaaag cacaaacga atcatccac aacagcccc 
      aatttaacg ttgcgcgtc gatcaagag acggcggga 
      agatatttt tgcaaagcc cacgttccc ggatttcct 
      gaaatcggt gcggatgca acaatttac ctcaaaggc 
      cctccgtct atagttagc caccgcact cagttcggc 
      atcgagggc gataatgtt cgcgtcgag tgcacgata 
      ttttccata ccgctgcca gagcgaatc gtctggaca 
      tttaacgga cgagagatc gacattcac gaccaagat 
      tatacgata ttggaagat ccctctcct gagggcatc 
      aaatctaca ttggtaata agggaatct caggagaaa 
      cattttggc atgtataat tgcagcgtc acgaatccg 
      tacggaagc gacgtcgtt gaaattaat ctgatgcaa 
      caaaaaaac gtcccgtca atcatcatc attttcggc 
      gtcgctgcg ctggttata ctcgtcttt attgtgatt 
      ttggtcgtt ttcctgtgc cagaaacag agcaagaag 
      aagccgcca ccagaaact gatgccaat acaatagaa 
      aaacaatgc aaagaatct gacagaagt tccaatatc 
      tccgacttg aaactggat ctgaggacg ggctcttca 
      aacagcaat gtgcactgt gacatggat tatattccc 
      ggggcggag tccgaaaca ggaagtgag tctgttata 
      acgcgaatc ggtgtccct ttagctggc cccgtcaat 
      gtttcaggg caagaaatc taccgctac tccgccgat 
      tacaccgaa ccaagcttc ccgcctaaa gacggccag 
      aacaacaac ggctacgtg ccatacgtg gactactcg 
      cgggactac aaccccccg atgatgcag ccccccatc 
      tcgtcctcg ctggacccc gggcgcgag agcctgcag 
      tccacgcgc ctgcagctc aactcgctg caggcgcac 
      caggtgtcc gggcagaac ctgccgttg aacgacctc 
      tccgacctg cacctggcg cagggcgtg cccaacggg 
      gggctgccg gggctgggg cccatcgac ccgcgcttc 
      agcgccacc tacggcaac ccctacctg cgcaacccc 
      aacgtgggg ctgcccacc cccaacacc gccaacccg 
      gcggccacg cccgcgccg cccccctac tcgcgggac 
      agctcccgg atagctagt tctagtgct atgatgact 
      gccagtgcg aacgtggcg aacacggtg gtggcgaac 
      ggtgccaac gggtcggtg ccccaggtg acgcggctt 
      ccgaactcc cccacgagt caatacata gttcccaat 
      tcgaacatg gcaacgatc aagagaggt actatggcc 
      acgcacgta tagtgcgct gggacttaa cgcaaaaga 
      gaagactga agcctgtat caatcgaaa tttttcact 
      acttgtttt ggtaggatt ttacgccga aattcgtgt 
      acatgtcta gacaattaa attaattga ccgaattat 
      tgattaaaa cttaatcag agcgtacag tactgctag 
      cctgttact cagcctcag tttgaatac aagtgacgt 
      ctcagaacg gaacatatc atattgata tcattagca 
      atcggacga attgaatga tgcaatttt tacgtgcaa 
      tacgcgaaa ttgactatt ttttattac ttgtatttg 
      aatatggag acaggtttt catattacc ttagactag 
      tgtacatat caaggtcag cagacgcgt ttttaacat 
      gtcaattct taaaatctt tcaaaattt ctacgattc 
      agtgtcata ctacttgaa aatttcgtt gtctagagg 
      gagattaac ctgccgttg tgacgagaa atattaatc 
      atctatgcg cttccagat aatatgtaa aacatgtct 
      gtgttcgtt ttatttcat tccagattt tgttacgtc 
      ttaggagaa tttattttt ttcatggtt tcgctaaat 
      tgtgagtag ctcggcttt ttatggcac actgttatg 
      aactattga aagaaataa atttgttat gaatggaaa 
      ttttagata aatatacca aagatattt tttgtatat 
      tactataac aaatgtgta tatttttaa gtgagtcca 
      acgacgcat ccactgatt tttataccg tatcgtact 
      tgcattttg tccttggtg caattcatt tactccttg 
      tacttaaat gtatgcaat tcgttgctt tactatttg 
      ctcattttt tattttgta tttacttgt atatttgta 
      tattttgct gtacatttt taataccta tcaaatttg 
      attacatat cagttacgt tatcttcca gtctaattt 
      gcatgtttt gtgtaaatg acgttttat cagtttgta 
      gaacgaatg agtgatatg ttataaagg atgatagat 
      ttagctcaa cttgtacat atagaattt tacgacgtt 
      ttctatgct agaaaacac tgttactac tgtgtataa 
      attttttta aatcttaac gaacagcaa tatttataa 
      aactttgtg attgtaaaa taaatatgc ctacttatg
    • >TC002914 RB
      cgccgcccg tctgagatg ctttgaagc agagcaatt 
      atttagaga taaaataag acaatcaag atggcatca 
      tcgcacaag ttcctgctg ctcctcctg cggatggcc 
      ggtctgtgg cttgtgaat gcagcagga gcgctccgc 
      gaacaaaag ttcgcaatg gagccccaa gaccagacg 
      gccgtggaa gggtccagg gtgacgctg ccctgcaga 
      gttatcgac aagagcggc atcctgcag tggaccaag 
      gacgacttc ggcctgggc acgcacagg aatctcagc 
      ggattcgat cgctactcc atgattggc agcgatgaa 
      gaaggcgac tattccctg gacatctac cccgtgacg 
      ttggacgac gacgccaaa taccaatgc caagtgtct 
      cccggtccc cagggccag ccgggaatt cgcagccat 
      tacgcgacg gtgactgtg ctcgtgccc cccgatccg 
      cccaagatc gtgcaaggg gattacctg gtcacgacg 
      gaagacagg gaaatagaa ctggagtgc ataagtcac 
      ggcgggaag ccggcggcg gaaataacc tggattgat 
      ggcctggga aacgtccta acagacgga attgaatat 
      atcgtcgag gagctatca gacggcagg agattcacg 
      gccaagtca atactcaaa ctgacgccc agaaaggac 
      caccacaac accaccttc acctgccag gcccagaac 
      acggccgag cggacgcac cggtccgcc aaactcaag 
      ctggaggta aaatatgcg ccgaaagtc acggtctct 
      atcctggcg ggagccacg cagagcgga aggattcct 
      gagggggcg gaggtcaga ctggggtgt catgcggat 
      gctaaccct gcagacgtc acctacagg tggttcttg 
      aacgatgaa ccgttaatt gggggatac accaccgag 
      atggttatt cacaacgcg accaggaaa tatcacgac 
      gctattgtc aaatgcgaa gtgcacaat gcagttggc 
      aagagcgag gagagcgaa acgctggat attagttac 
      ggtccccag tttcgcaca aagccgcgt tcagtgcag 
      gccgacctg ggcacaagc gtaactctg acctgcgac 
      gtggacggt aatcccctt ccggagata tcgtggttc 
      gacgaaaag cacaaacga atcatccac aacagcccc 
      aatttaacg ttgcgcgtc gatcaagag acggcggga 
      agatatttt tgcaaagcc cacgttccc ggatttcct 
      gaaatcggt gcggatgca acaatttac ctcaaaggc 
      cctccgtct atagttagc caccgcact cagttcggc 
      atcgagggc gataatgtt cgcgtcgag tgcacgata 
      ttttccata ccgctgcca gagcgaatc gtctggaca 
      tttaacgga cgagagatc gacattcac gaccaagat 
      tatacgata ttggaagat ccctctcct gagggcatc 
      aaatctaca ttggtaata agggaatct caggagaaa 
      cattttggc atgtataat tgcagcgtc acgaatccg 
      tacggaagc gacgtcgtt gaaattaat ctgatgcaa 
      caaaaaaac gtcccgtca atcatcatc attttcggc 
      gtcgctgcg ctggttata ctcgtcttt attgtgatt 
      ttggtcgtt ttcctgtgc cagaaacag agcaagaag 
      aagccgcca ccagaaact gatgccaat acaatagaa 
      aaacaatgc aaagaatct gacagaagt tccaatatc 
      tccgacttg aaactggat ctgaggacg ggctcttca 
      aacagcaat gtgcactgt gacatggat tatattccc 
      ggggcggag tccgaaaca ggaaggcaa gaaatctac 
      cgctactcc gccgattac accgaacca agcttcccg 
      cctaaagac ggccagaac aacaacggc tacgtgcca 
      tacgtggac tactcgcgg gactacaac cccccgatg 
      atgcagccc cccatctcg tcctcgctg gaccccggg 
      cgcgagagc ctgcagtcc acgcgcctg cagctcaac 
      tcgctgcag gcgcaccag gtgtccggg cagaacctg 
      ccgttgaac gacctctcc gacctgcac ctggcgcag 
      ggcgtgccc aacgggggg ctgccgggg ctggggccc 
      atcgacccg cgcttcagc gccacctac ggcaacccc 
      tacctgcgc aaccccaac gtggggctg cccaccccc 
      aacaccgcc aacccggcg gccacgccc gcgccgccc 
      ccctactcg cgggacagc tcccggata gctagttct 
      agtgctatg atgactgcc agtgcgaac gtggcgaac 
      acggtggtg gcgaacggt gccaacggg tcggtgccc 
      caggtgacg cggcttccg aactccccc acgagtcaa 
      tacatagtt cccaattcg aacatggca acgatcaag 
      agaggtact atggccacg cacgtatag tgcgctggg 
      acttaacgc aaaagagaa gactgaagc ctgtatcaa 
      tcgaaattt ttcactact tgttttggt aggatttta 
      cgccgaaat tcgtgtaca tgtctagac aattaaatt 
      aattgaccg aattattga ttaaaactt aatcagagc 
      gtacagtac tgctagcct gttactcag cctcagttt 
      gaatacaag tgacgtctc agaacggaa catatcata 
      ttgatatca ttagcaatc ggacgaatt gaatgatgc 
      aatttttac gtgcaatac gcgaaattg actattttt 
      tattacttg tatttgaat atggagaca ggttttcat 
      attacctta gactagtgt acatatcaa ggtcagcag 
      acgcgtttt taacatgtc aattcttaa aatctttca 
      aaatttcta cgattcagt gtcatacta cttgaaaat 
      ttcgttgtc tagagggag attaacctg ccgttgtga 
      cgagaaata ttaatcatc tatgcgctt ccagataat 
      atgtaaaac atgtctgtg ttcgtttta tttcattcc 
      agattttgt tacgtctta ggagaattt atttttttc 
      atggtttcg ctaaattgt gagtagctc ggcttttta 
      tggcacact gttatgaac tattgaaag aaataaatt 
      tgttatgaa tggaaattt tagataaat ataccaaag 
      atatttttt gtatattac tataacaaa tgtgtatat 
      ttttaagtg agtccaacg acgcatcca ctgattttt 
      ataccgtat cgtacttgc attttgtcc ttggtgcaa 
      ttcatttac tccttgtac ttaaatgta tgcaattcg 
      ttgctttac tatttgctc attttttat tttgtattt 
      acttgtata tttgtatat tttgctgta catttttaa 
      tacctatca aatttgatt acatatcag ttacgttat 
      cttccagtc taatttgca tgttttgtg taaatgacg 
      ttttatcag tttgtagaa cgaatgagt gatatgtta 
      taaaggatg atagattta gctcaactt gtacatata 
      gaattttac gacgttttc tatgctaga aaacactgt 
      tactactgt gtataaatt tttttaaat cttaacgaa 
      cagcaatat ttataaaac tttgtgatt gtaaaataa 
      atatgccta cttatg
    • >TC002914 RC
      cgccgcccg tctgagatg ctttgaagc agagcaatt 
      atttagaga taaaataag acaatcaag atggcatca 
      tcgcacaag ttcctgctg ctcctcctg cggatggcc 
      ggtctgtgg cttgtgaat gcagcagga gcgctccgc 
      gaacaaaag ttcgcaatg gagccccaa gaccagacg 
      gccgtggaa gggtccagg gtgacgctg ccctgcaga 
      gttatcgac aagagcggc atcctgcag tggaccaag 
      gacgacttc ggcctgggc acgcacagg aatctcagc 
      ggattcgat cgctactcc atgattggc agcgatgaa 
      gaaggcgac tattccctg gacatctac cccgtgacg 
      ttggacgac gacgccaaa taccaatgc caagtgtct 
      cccggtccc cagggccag ccgggaatt cgcagccat 
      tacgcgacg gtgactgtg ctcgtgccc cccgatccg 
      cccaagatc gtgcaaggg gattacctg gtcacgacg 
      gaagacagg gaaatagaa ctggagtgc ataagtcac 
      ggcgggaag ccggcggcg gaaataacc tggattgat 
      ggcctggga aacgtccta acagacgga attgaatat 
      atcgtcgag gagctatca gacggcagg agattcacg 
      gccaagtca atactcaaa ctgacgccc agaaaggac 
      caccacaac accaccttc acctgccag gcccagaac 
      acggccgag cggacgcac cggtccgcc aaactcaag 
      ctggaggta aaatatgcg ccgaaagtc acggtctct 
      atcctggcg ggagccacg cagagcgga aggattcct 
      gagggggcg gaggtcaga ctggggtgt catgcggat 
      gctaaccct gcagacgtc acctacagg tggttcttg 
      aacgatgaa ccgttaatt gggggatac accaccgag 
      atggttatt cacaacgcg accaggaaa tatcacgac 
      gctattgtc aaatgcgaa gtgcacaat gcagttggc 
      aagagcgag gagagcgaa acgctggat attagttac 
      ggtccccag tttcgcaca aagccgcgt tcagtgcag 
      gccgacctg ggcacaagc gtaactctg acctgcgac 
      gtggacggt aatcccctt ccggagata tcgtggttc 
      gacgaaaag cacaaacga atcatccac aacagcccc 
      aatttaacg ttgcgcgtc gatcaagag acggcggga 
      agatatttt tgcaaagcc cacgttccc ggatttcct 
      gaaatcggt gcggatgca acaatttac ctcaaaggc 
      cctccgtct atagttagc caccgcact cagttcggc 
      atcgagggc gataatgtt cgcgtcgag tgcacgata 
      ttttccata ccgctgcca gagcgaatc gtctggaca 
      tttaacgga cgagagatc gacattcac gaccaagat 
      tatacgata ttggaagat ccctctcct gagggcatc 
      aaatctaca ttggtaata agggaatct caggagaaa 
      cattttggc atgtataat tgcagcgtc acgaatccg 
      tacggaagc gacgtcgtt gaaattaat ctgatgcaa 
      caaaaaaac gtcccgtca atcatcatc attttcggc 
      gtcgctgcg ctggttata ctcgtcttt attgtgatt 
      ttggtcgtt ttcctgtgc cagaaacag agcaagaag 
      aagccgcca ccagaaaaa aaacaatgc aaagaatct 
      gacagaagt tccaatatc tccgacttg aaactggat 
      ctgaggacg ggctcttca aacagcaat gtgcactgt 
      gacatggat tatattccc ggggcggag tccgaaaca 
      ggaagtgag tctgttata acgcgaatc ggtgtccct 
      ttagctggc cccgtcaat gtttcaggg caagaaatc 
      taccgctac tccgccgat tacaccgaa ccaagcttc 
      ccgcctaaa gacggccag aacaacaac ggctacgtg 
      ccatacgtg gactactcg cgggactac aaccccccg 
      atgatgcag ccccccatc tcgtcctcg ctggacccc 
      gggcgcgag agcctgcag tccacgcgc ctgcagctc 
      aactcgctg caggcgcac caggtgtcc gggcagaac 
      ctgccgttg aacgacctc tccgacctg cacctggcg 
      cagggcgtg cccaacggg gggctgccg gggctgggg 
      cccatcgac ccgcgcttc agcgccacc tacggcaac 
      ccctacctg cgcaacccc aacgtgggg ctgcccacc 
      cccaacacc gccaacccg gcggccacg cccgcgccg 
      cccccctac tcgcgggac agctcccgg atagctagt 
      tctagtgct atgatgact gccagtgcg aacgtggcg 
      aacacggtg gtggcgaac ggtgccaac gggtcggtg 
      ccccaggtg acgcggctt ccgaactcc cccacgagt 
      caatacata gttcccaat tcgaacatg gcaacgatc 
      aagagaggt actatggcc acgcacgta tagtgcgct 
      gggacttaa cgcaaaaga gaagactga agcctgtat 
      caatcgaaa tttttcact acttgtttt ggtaggatt 
      ttacgccga aattcgtgt acatgtcta gacaattaa 
      attaattga ccgaattat tgattaaaa cttaatcag 
      agcgtacag tactgctag cctgttact cagcctcag 
      tttgaatac aagtgacgt ctcagaacg gaacatatc 
      atattgata tcattagca atcggacga attgaatga 
      tgcaatttt tacgtgcaa tacgcgaaa ttgactatt 
      ttttattac ttgtatttg aatatggag acaggtttt 
      catattacc ttagactag tgtacatat caaggtcag 
      cagacgcgt ttttaacat gtcaattct taaaatctt 
      tcaaaattt ctacgattc agtgtcata ctacttgaa 
      aatttcgtt gtctagagg gagattaac ctgccgttg 
      tgacgagaa atattaatc atctatgcg cttccagat 
      aatatgtaa aacatgtct gtgttcgtt ttatttcat 
      tccagattt tgttacgtc ttaggagaa tttattttt 
      ttcatggtt tcgctaaat tgtgagtag ctcggcttt 
      ttatggcac actgttatg aactattga aagaaataa 
      atttgttat gaatggaaa ttttagata aatatacca 
      aagatattt tttgtatat tactataac aaatgtgta 
      tatttttaa gtgagtcca acgacgcat ccactgatt 
      tttataccg tatcgtact tgcattttg tccttggtg 
      caattcatt tactccttg tacttaaat gtatgcaat 
      tcgttgctt tactatttg ctcattttt tattttgta 
      tttacttgt atatttgta tattttgct gtacatttt 
      taataccta tcaaatttg attacatat cagttacgt 
      tatcttcca gtctaattt gcatgtttt gtgtaaatg 
      acgttttat cagtttgta gaacgaatg agtgatatg 
      ttataaagg atgatagat ttagctcaa cttgtacat 
      atagaattt tacgacgtt ttctatgct agaaaacac 
      tgttactac tgtgtataa attttttta aatcttaac 
      gaacagcaa tatttataa aactttgtg attgtaaaa 
      taaatatgc ctacttatg