| General information | |||
| TC002906 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 6757483..6796601 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002906 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03467 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC002906 RA
METEELIKL VDGIYKNIL DKFNPGARQ MINAGKAYL KALHGAAAA SRMYVDAIN KLARQAQQG TWGGSSDIG AALMKIVEV YKEIQDQQM NILKAFYVD LLVPLETNL EKDTKVVQS EQKRFLQQH KQRSETYSK AAAMMKKQR KKSRTSQKT GLAMDKELK NMQVLEEEK TKLDAFCEQ SLKNAITQE RRRYGFVLE RQCSLAKHY LSFHNHGLA AYQQNLEKW SEVAKTREY LPESVENIF TNRLRQISV WQDEDVYSN PRSPNLEDD RISISSQLR KTKSMDASC LDIRSIGDV GSPVTTLSR AKSEYNLNS STHSLAHEG HTPAHRPKS IAVPPPPQW ETQLARALY AYLSSGDNQ LSFLEGDLI ALIGERNKG WQFGENLRT QCSGWFPLA YTELLDDPT TSPTHRSDA GGSVQSQQT PTNGGNPMS LGGSGSPSG NNSTLDPPA PRMFGDTLH LHRSSNNAK QIRRAIGSN NIPPPALPA PVPTPSLPF QKIGISQSQ SANFKSSAD TSSFQSPYQ TGIKSSTST FSFSTPPTG TYSTHPQPA RADRPTAGA PLPLHLQSK SGKIGGPVG NVSLHSSND SGFSNDPPP QPEIDYSDD DSISGQTKL PARRVRRQP NSDGNNNTV QKNVRQANS HGNLIDNRY YMSDDQDAT RERKGLVKR TKSFWKFGK NSSDNEILE GMALWRHRD LVDVEEEKI KRKNRFNSQ ERIKKPSRD RSNDSDRTI NANQNIESE NKKEVEQRK NVENARQSF REKPRHPDL DKENYGYHK RNNKPANDQ FYDDDGDGL IMKTVNRKN ILQQYNNDS SVGDSDSDS EITSDDPYD CILVDDQNV NKRENHFPN VVEIGKKLE KLSKSSKFS PNNGNNRHS VAEKNLRNN FEKNLTSDN HNLIQYEKQ HSFKTFGVE SPEPDDNER YQSRNKRNN ETNVQEKRR YYADAPKRN NSNEKQTRP SYESIDSDV DKRREGRSS RRSNSETEP RDKKYYDSA NDELSDAAE NRQIIPRTK LTKSNSHHS EENGLMGYG ETLQRRLKK LEYGTKYDE KSAHNGNMY GPWYDLWGL DASARK
- >TC002906 RA
atggagact gaagaactc atcaaactc gttgatgga atttacaag aatatcctg gataagttt aatccaggt gcaagacaa atgatcaac gctggaaaa gcgtacctc aaagctctc cacggtgct gcggcagca tccagaatg tatgtagat gcaattaat aagctcgct cgtcaagcg caacaagga acctggggt ggatcctca gacatcgga gccgcccta atgaaaatt gtcgaggtt tacaaagag attcaagac caacaaatg aacatactt aaagcattt tacgtcgac ttgctcgtt ccactcgag accaatttg gaaaaggat accaaagtt gtacagtcc gagcagaag agattttta cagcagcac aagcagcgg tcggagact tacagtaaa gccgctgct atgatgaaa aagcaacgg aagaaatcg agaacttcg caaaaaacg ggactagcc atggataag gaactaaag aatatgcaa gtcctggaa gaggagaaa actaaattg gacgctttc tgtgagcag agtcttaaa aacgcaata acccaggag cgcagacgt tacggattt gttcttgaa aggcagtgc tctctggcg aaacattat ttatctttt cacaatcac ggactggct gcataccaa caaaatctg gagaaatgg tcggaagtt gccaaaaca agggaatat cttccggaa tctgtcgaa aacattttc acaaacaga ctaaggcaa atatcagtc tggcaggac gaggacgtg tactcgaac ccaaggagt cctaatctc gaagacgat cgaatcagt attagctct cagttacgg aaaaccaag agcatggac gcgtcctgt cttgatata cgttcaatc ggcgacgtc gggtcccca gtgacgacg ctatcaagg gccaaatcg gagtataat ttaaattct tcgacacat tcacttgca cacgagggc cacactcca gcccatcgg cccaaatcg atcgctgtc ccaccgcca ccgcagtgg gaaacgcaa ctagctcga gcgctttat gcctacctt tccagtgga gataatcaa ctcagtttt cttgaagga gatctcatc gctctgata ggggagcga aataagggc tggcaattc ggagagaac ttgaggaca caatgcagt ggatggttc ccattagca tacactgaa cttctagat gatcctacc acgtcgcca actcatcgc agtgatgcc ggtggatct gttcaaagt cagcagacg ccaacaaac ggcggcaac cccatgtcg ctcggcggc tccggctct ccttcgggt aacaattcg acgctagac cctccagcc cctcgtatg ttcggcgac accctccat ttgcataga tcctccaat aacgctaaa cagattcgg cgagcgatc ggttctaac aacattccg cccccggct ttgccggct ccggtgccg accccctcc ttgccattc cagaagatc ggcatttca caatcacaa agtgccaac ttcaagtcc agcgccgac acgtctagc ttccaatcc ccgtaccaa acagggata aaatcatca acatccacc tttagtttc tcgacacct cccacagga acctactcc acgcacccc cagcccgcc cgggccgac cggcccacg gcgggcgct cctctgccg ctgcatctg caaagcaag agcggaaaa atcggcggt cccgtcgga aacgtatcg ttacacagc agtaacgat tccgggttc agcaacgat ccgccgcca caaccagag attgattat tcagatgac gactccatt tctggacaa actaaattg ccggcgaga agagtccgt cgccagcca aacagtgac ggtaataat aacacagtg caaaagaat gtaagacag gcaaacagt cacgggaat ttgatcgac aacagatat tacatgtcc gatgatcaa gatgcgact agagagcgt aaaggtcta gttaagaga actaagtcg ttctggaag tttggcaaa aacagttcg gataatgag attttagaa ggcatggcc ttgtggagg catagggac cttgtggac gtggaagaa gagaaaatt aaacgaaag aacaggttt aatagtcaa gagagaatt aaaaaacct agtcgtgac agatcaaac gattcagat cgaacaatt aacgccaac caaaatata gaaagtgag aataaaaag gaagttgag cagagaaaa aacgtagaa aacgcgaga caatccttt agggaaaaa ccaagacat cctgacttg gacaaggaa aattacggt taccacaag cgaaataat aaaccggca aatgaccag ttttacgac gatgatggt gatggactg attatgaaa acagttaat cgcaagaac attttgcaa cagtacaat aacgactcc tccgtgggc gattccgat tccgattcg gaaatcaca agcgacgat ccgtacgac tgtattctg gtggacgac cagaacgta aacaagcgc gaaaaccac tttcccaac gttgtggaa atcggcaag aagctggaa aagctgtca aaatcgagc aagttctcg cccaacaac ggcaacaac cgccattca gtggcggag aaaaatttg cgcaacaat ttcgaaaag aatctcacc tcggacaac cacaacctg atccagtac gagaagcag cacagcttc aaaactttc ggagtggaa tctccggag ccggacgac aacgagcgc tatcagagt cgcaataaa agaaataat gaaactaat gtccaggaa aaacgccga tattatgcc gatgcgccc aaacgcaat aactcaaac gaaaaacaa actagaccg agttacgaa agtatcgat tcggacgtg gacaagcgg cgggagggc cgcagcagc cgccgatcg aattcggaa acggagccc cgagataaa aaatattac gactcggcc aatgacgag ttgtcggac gccgcggaa aacaggcag attattccg cggacgaag ctgactaag agcaatagt caccactcg gaggagaac gggctcatg gggtacggg gagacgctg cagaggcgc ttgaaaaag ctggaatac gggaccaag tacgacgaa aagtcggca cataacggg aacatgtac gggccttgg tatgacttg tggggcctt gacgcttcc gcccgaaaa taa
- >TC002906 RA
catgccgcg catgagaaa cgctgcaat gcgcgagtt ctgggcggg tgcgattag aaagtgctg ttcccgtgg cgaagtcaa cgcgcgcga tttagtctt tacgtggac tctgcgact gtttgcagg actttgtat ttccgatgt ggaccgaat cacgtgcat cgcaaagtt tgtgaaatt cgtgccaaa ggtgccagc gatttttta cgcgattta ttgattgga tcgagtccg gggctcaat cttcgttag aaagacaat ttaagcccg gattctgca tcattccct ccgtgcaat taaagaagt gggatatgt gttaaataa attagcgca aatagtggt ttaaattcg aaaagagag aatcggtgc tgtttccgt gcttgtgcc atgaacccc aactaactt tgtcctctg agcaacgtc ataaaactg cttcgacat ggagactga agaactcat caaactcgt tgatggaat ttacaagaa tatcctgga taagtttaa tccaggtgc aagacaaat gatcaacgc tggaaaagc gtacctcaa agctctcca cggtgctgc ggcagcatc cagaatgta tgtagatgc aattaataa gctcgctcg tcaagcgca acaaggaac ctggggtgg atcctcaga catcggagc cgccctaat gaaaattgt cgaggttta caaagagat tcaagacca acaaatgaa catacttaa agcatttta cgtcgactt gctcgttcc actcgagac caatttgga aaaggatac caaagttgt acagtccga gcagaagag atttttaca gcagcacaa gcagcggtc ggagactta cagtaaagc cgctgctat gatgaaaaa gcaacggaa gaaatcgag aacttcgca aaaaacggg actagccat ggataagga actaaagaa tatgcaagt cctggaaga ggagaaaac taaattgga cgctttctg tgagcagag tcttaaaaa cgcaataac ccaggagcg cagacgtta cggatttgt tcttgaaag gcagtgctc tctggcgaa acattattt atcttttca caatcacgg actggctgc ataccaaca aaatctgga gaaatggtc ggaagttgc caaaacaag ggaatatct tccggaatc tgtcgaaaa cattttcac aaacagact aaggcaaat atcagtctg gcaggacga ggacgtgta ctcgaaccc aaggagtcc taatctcga agacgatcg aatcagtat tagctctca gttacggaa aaccaagag catggacgc gtcctgtct tgatatacg ttcaatcgg cgacgtcgg gtccccagt gacgacgct atcaagggc caaatcgga gtataattt aaattcttc gacacattc acttgcaca cgagggcca cactccagc ccatcggcc caaatcgat cgctgtccc accgccacc gcagtggga aacgcaact agctcgagc gctttatgc ctacctttc cagtggaga taatcaact cagttttct tgaaggaga tctcatcgc tctgatagg ggagcgaaa taagggctg gcaattcgg agagaactt gaggacaca atgcagtgg atggttccc attagcata cactgaact tctagatga tcctaccac gtcgccaac tcatcgcag tgatgccgg tggatctgt tcaaagtca gcagacgcc aacaaacgg cggcaaccc catgtcgct cggcggctc cggctctcc ttcgggtaa caattcgac gctagaccc tccagcccc tcgtatgtt cggcgacac cctccattt gcatagatc ctccaataa cgctaaaca gattcggcg agcgatcgg ttctaacaa cattccgcc cccggcttt gccggctcc ggtgccgac cccctcctt gccattcca gaagatcgg catttcaca atcacaaag tgccaactt caagtccag cgccgacac gtctagctt ccaatcccc gtaccaaac agggataaa atcatcaac atccacctt tagtttctc gacacctcc cacaggaac ctactccac gcaccccca gcccgcccg ggccgaccg gcccacggc gggcgctcc tctgccgct gcatctgca aagcaagag cggaaaaat cggcggtcc cgtcggaaa cgtatcgtt acacagcag taacgattc cgggttcag caacgatcc gccgccaca accagagat tgattattc agatgacga ctccatttc tggacaaac taaattgcc ggcgagaag agtccgtcg ccagccaaa cagtgacgg taataataa cacagtgca aaagaatgt aagacaggc aaacagtca cgggaattt gatcgacaa cagatatta catgtccga tgatcaaga tgcgactag agagcgtaa aggtctagt taagagaac taagtcgtt ctggaagtt tggcaaaaa cagttcgga taatgagat tttagaagg catggcctt gtggaggca tagggacct tgtggacgt ggaagaaga gaaaattaa acgaaagaa caggtttaa tagtcaaga gagaattaa aaaacctag tcgtgacag atcaaacga ttcagatcg aacaattaa cgccaacca aaatataga aagtgagaa taaaaagga agttgagca gagaaaaaa cgtagaaaa cgcgagaca atcctttag ggaaaaacc aagacatcc tgacttgga caaggaaaa ttacggtta ccacaagcg aaataataa accggcaaa tgaccagtt ttacgacga tgatggtga tggactgat tatgaaaac agttaatcg caagaacat tttgcaaca gtacaataa cgactcctc cgtgggcga ttccgattc cgattcgga aatcacaag cgacgatcc gtacgactg tattctggt ggacgacca gaacgtaaa caagcgcga aaaccactt tcccaacgt tgtggaaat cggcaagaa gctggaaaa gctgtcaaa atcgagcaa gttctcgcc caacaacgg caacaaccg ccattcagt ggcggagaa aaatttgcg caacaattt cgaaaagaa tctcacctc ggacaacca caacctgat ccagtacga gaagcagca cagcttcaa aactttcgg agtggaatc tccggagcc ggacgacaa cgagcgcta tcagagtcg caataaaag aaataatga aactaatgt ccaggaaaa acgccgata ttatgccga tgcgcccaa acgcaataa ctcaaacga aaaacaaac tagaccgag ttacgaaag tatcgattc ggacgtgga caagcggcg ggagggccg cagcagccg ccgatcgaa ttcggaaac ggagccccg agataaaaa atattacga ctcggccaa tgacgagtt gtcggacgc cgcggaaaa caggcagat tattccgcg gacgaagct gactaagag caatagtca ccactcgga ggagaacgg gctcatggg gtacgggga gacgctgca gaggcgctt gaaaaagct ggaatacgg gaccaagta cgacgaaaa gtcggcaca taacgggaa catgtacgg gccttggta tgacttgtg gggccttga cgcttccgc ccgaaaata actttctga ctttcttga atttttgca tgtctttga tcattgcac ggtttacgg ttttggctc tttttcagg tctaataac tttgccacc attaagctg cgcaaaact aatcctaac acagaccga aatgcttca cttatcctt gagtagatt ggcttcact actgttttc gttcttcac caacatacg ctagtctta attattact ctatacttg catgcgtta tacgggacg ttgttattg tacatatta tatttattg ttgtagcga tactcttat tttcactca ttttttgta catatctac ttttggtgg ttggggtgg tgcaataag gtttgaatg taatacaaa ctaactgta tttgcttcg taatttaaa tctatctat tgttaagtt gaataccgc cagctcaat cccttttaa tatgaaaca tttttaggg gctagtgaa atcagagac tgtgataaa cttacaact tttgtagtg tattatact aacgcattt agtaagatt gaaaaataa ttagcaaaa cacgtaatc atacactaa cgaatactc aataggtac atacatgtt atattgcca ctttttgaa taattataa tctttgacg aatgtttta ttgttgctg accgtccat tttgttaca tttagacat aatgttagt gttatagtg tggtaaatt aattgtatt aatatcatt aattattat tacctcgga atatataaa ggcaggggt tccctaggc tagaacttg aatgtaaca gtccactta accgtatat ttattagaa taaataata tactcgtag ctattgtct attgtaaac ctgacaaac aaataactt ggttttaca taaaaacag cagagttat tattgttgt acttaaaat gtatatcat tttcatata aataaagat ttatattgt tt
