Status
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Gene information
General information
TC002906 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6757483..6796601
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002906
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03467

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002906 RA
      METEELIKL VDGIYKNIL DKFNPGARQ MINAGKAYL 
      KALHGAAAA SRMYVDAIN KLARQAQQG TWGGSSDIG 
      AALMKIVEV YKEIQDQQM NILKAFYVD LLVPLETNL 
      EKDTKVVQS EQKRFLQQH KQRSETYSK AAAMMKKQR 
      KKSRTSQKT GLAMDKELK NMQVLEEEK TKLDAFCEQ 
      SLKNAITQE RRRYGFVLE RQCSLAKHY LSFHNHGLA 
      AYQQNLEKW SEVAKTREY LPESVENIF TNRLRQISV 
      WQDEDVYSN PRSPNLEDD RISISSQLR KTKSMDASC 
      LDIRSIGDV GSPVTTLSR AKSEYNLNS STHSLAHEG 
      HTPAHRPKS IAVPPPPQW ETQLARALY AYLSSGDNQ 
      LSFLEGDLI ALIGERNKG WQFGENLRT QCSGWFPLA 
      YTELLDDPT TSPTHRSDA GGSVQSQQT PTNGGNPMS 
      LGGSGSPSG NNSTLDPPA PRMFGDTLH LHRSSNNAK 
      QIRRAIGSN NIPPPALPA PVPTPSLPF QKIGISQSQ 
      SANFKSSAD TSSFQSPYQ TGIKSSTST FSFSTPPTG 
      TYSTHPQPA RADRPTAGA PLPLHLQSK SGKIGGPVG 
      NVSLHSSND SGFSNDPPP QPEIDYSDD DSISGQTKL 
      PARRVRRQP NSDGNNNTV QKNVRQANS HGNLIDNRY 
      YMSDDQDAT RERKGLVKR TKSFWKFGK NSSDNEILE 
      GMALWRHRD LVDVEEEKI KRKNRFNSQ ERIKKPSRD 
      RSNDSDRTI NANQNIESE NKKEVEQRK NVENARQSF 
      REKPRHPDL DKENYGYHK RNNKPANDQ FYDDDGDGL 
      IMKTVNRKN ILQQYNNDS SVGDSDSDS EITSDDPYD 
      CILVDDQNV NKRENHFPN VVEIGKKLE KLSKSSKFS 
      PNNGNNRHS VAEKNLRNN FEKNLTSDN HNLIQYEKQ 
      HSFKTFGVE SPEPDDNER YQSRNKRNN ETNVQEKRR 
      YYADAPKRN NSNEKQTRP SYESIDSDV DKRREGRSS 
      RRSNSETEP RDKKYYDSA NDELSDAAE NRQIIPRTK 
      LTKSNSHHS EENGLMGYG ETLQRRLKK LEYGTKYDE 
      KSAHNGNMY GPWYDLWGL DASARK
    CDS sequence
    • >TC002906 RA
      atggagact gaagaactc atcaaactc gttgatgga 
      atttacaag aatatcctg gataagttt aatccaggt 
      gcaagacaa atgatcaac gctggaaaa gcgtacctc 
      aaagctctc cacggtgct gcggcagca tccagaatg 
      tatgtagat gcaattaat aagctcgct cgtcaagcg 
      caacaagga acctggggt ggatcctca gacatcgga 
      gccgcccta atgaaaatt gtcgaggtt tacaaagag 
      attcaagac caacaaatg aacatactt aaagcattt 
      tacgtcgac ttgctcgtt ccactcgag accaatttg 
      gaaaaggat accaaagtt gtacagtcc gagcagaag 
      agattttta cagcagcac aagcagcgg tcggagact 
      tacagtaaa gccgctgct atgatgaaa aagcaacgg 
      aagaaatcg agaacttcg caaaaaacg ggactagcc 
      atggataag gaactaaag aatatgcaa gtcctggaa 
      gaggagaaa actaaattg gacgctttc tgtgagcag 
      agtcttaaa aacgcaata acccaggag cgcagacgt 
      tacggattt gttcttgaa aggcagtgc tctctggcg 
      aaacattat ttatctttt cacaatcac ggactggct 
      gcataccaa caaaatctg gagaaatgg tcggaagtt 
      gccaaaaca agggaatat cttccggaa tctgtcgaa 
      aacattttc acaaacaga ctaaggcaa atatcagtc 
      tggcaggac gaggacgtg tactcgaac ccaaggagt 
      cctaatctc gaagacgat cgaatcagt attagctct 
      cagttacgg aaaaccaag agcatggac gcgtcctgt 
      cttgatata cgttcaatc ggcgacgtc gggtcccca 
      gtgacgacg ctatcaagg gccaaatcg gagtataat 
      ttaaattct tcgacacat tcacttgca cacgagggc 
      cacactcca gcccatcgg cccaaatcg atcgctgtc 
      ccaccgcca ccgcagtgg gaaacgcaa ctagctcga 
      gcgctttat gcctacctt tccagtgga gataatcaa 
      ctcagtttt cttgaagga gatctcatc gctctgata 
      ggggagcga aataagggc tggcaattc ggagagaac 
      ttgaggaca caatgcagt ggatggttc ccattagca 
      tacactgaa cttctagat gatcctacc acgtcgcca 
      actcatcgc agtgatgcc ggtggatct gttcaaagt 
      cagcagacg ccaacaaac ggcggcaac cccatgtcg 
      ctcggcggc tccggctct ccttcgggt aacaattcg 
      acgctagac cctccagcc cctcgtatg ttcggcgac 
      accctccat ttgcataga tcctccaat aacgctaaa 
      cagattcgg cgagcgatc ggttctaac aacattccg 
      cccccggct ttgccggct ccggtgccg accccctcc 
      ttgccattc cagaagatc ggcatttca caatcacaa 
      agtgccaac ttcaagtcc agcgccgac acgtctagc 
      ttccaatcc ccgtaccaa acagggata aaatcatca 
      acatccacc tttagtttc tcgacacct cccacagga 
      acctactcc acgcacccc cagcccgcc cgggccgac 
      cggcccacg gcgggcgct cctctgccg ctgcatctg 
      caaagcaag agcggaaaa atcggcggt cccgtcgga 
      aacgtatcg ttacacagc agtaacgat tccgggttc 
      agcaacgat ccgccgcca caaccagag attgattat 
      tcagatgac gactccatt tctggacaa actaaattg 
      ccggcgaga agagtccgt cgccagcca aacagtgac 
      ggtaataat aacacagtg caaaagaat gtaagacag 
      gcaaacagt cacgggaat ttgatcgac aacagatat 
      tacatgtcc gatgatcaa gatgcgact agagagcgt 
      aaaggtcta gttaagaga actaagtcg ttctggaag 
      tttggcaaa aacagttcg gataatgag attttagaa 
      ggcatggcc ttgtggagg catagggac cttgtggac 
      gtggaagaa gagaaaatt aaacgaaag aacaggttt 
      aatagtcaa gagagaatt aaaaaacct agtcgtgac 
      agatcaaac gattcagat cgaacaatt aacgccaac 
      caaaatata gaaagtgag aataaaaag gaagttgag 
      cagagaaaa aacgtagaa aacgcgaga caatccttt 
      agggaaaaa ccaagacat cctgacttg gacaaggaa 
      aattacggt taccacaag cgaaataat aaaccggca 
      aatgaccag ttttacgac gatgatggt gatggactg 
      attatgaaa acagttaat cgcaagaac attttgcaa 
      cagtacaat aacgactcc tccgtgggc gattccgat 
      tccgattcg gaaatcaca agcgacgat ccgtacgac 
      tgtattctg gtggacgac cagaacgta aacaagcgc 
      gaaaaccac tttcccaac gttgtggaa atcggcaag 
      aagctggaa aagctgtca aaatcgagc aagttctcg 
      cccaacaac ggcaacaac cgccattca gtggcggag 
      aaaaatttg cgcaacaat ttcgaaaag aatctcacc 
      tcggacaac cacaacctg atccagtac gagaagcag 
      cacagcttc aaaactttc ggagtggaa tctccggag 
      ccggacgac aacgagcgc tatcagagt cgcaataaa 
      agaaataat gaaactaat gtccaggaa aaacgccga 
      tattatgcc gatgcgccc aaacgcaat aactcaaac 
      gaaaaacaa actagaccg agttacgaa agtatcgat 
      tcggacgtg gacaagcgg cgggagggc cgcagcagc 
      cgccgatcg aattcggaa acggagccc cgagataaa 
      aaatattac gactcggcc aatgacgag ttgtcggac 
      gccgcggaa aacaggcag attattccg cggacgaag 
      ctgactaag agcaatagt caccactcg gaggagaac 
      gggctcatg gggtacggg gagacgctg cagaggcgc 
      ttgaaaaag ctggaatac gggaccaag tacgacgaa 
      aagtcggca cataacggg aacatgtac gggccttgg 
      tatgacttg tggggcctt gacgcttcc gcccgaaaa 
      taa
    mRNA sequence
    • >TC002906 RA
      catgccgcg catgagaaa cgctgcaat gcgcgagtt 
      ctgggcggg tgcgattag aaagtgctg ttcccgtgg 
      cgaagtcaa cgcgcgcga tttagtctt tacgtggac 
      tctgcgact gtttgcagg actttgtat ttccgatgt 
      ggaccgaat cacgtgcat cgcaaagtt tgtgaaatt 
      cgtgccaaa ggtgccagc gatttttta cgcgattta 
      ttgattgga tcgagtccg gggctcaat cttcgttag 
      aaagacaat ttaagcccg gattctgca tcattccct 
      ccgtgcaat taaagaagt gggatatgt gttaaataa 
      attagcgca aatagtggt ttaaattcg aaaagagag 
      aatcggtgc tgtttccgt gcttgtgcc atgaacccc 
      aactaactt tgtcctctg agcaacgtc ataaaactg 
      cttcgacat ggagactga agaactcat caaactcgt 
      tgatggaat ttacaagaa tatcctgga taagtttaa 
      tccaggtgc aagacaaat gatcaacgc tggaaaagc 
      gtacctcaa agctctcca cggtgctgc ggcagcatc 
      cagaatgta tgtagatgc aattaataa gctcgctcg 
      tcaagcgca acaaggaac ctggggtgg atcctcaga 
      catcggagc cgccctaat gaaaattgt cgaggttta 
      caaagagat tcaagacca acaaatgaa catacttaa 
      agcatttta cgtcgactt gctcgttcc actcgagac 
      caatttgga aaaggatac caaagttgt acagtccga 
      gcagaagag atttttaca gcagcacaa gcagcggtc 
      ggagactta cagtaaagc cgctgctat gatgaaaaa 
      gcaacggaa gaaatcgag aacttcgca aaaaacggg 
      actagccat ggataagga actaaagaa tatgcaagt 
      cctggaaga ggagaaaac taaattgga cgctttctg 
      tgagcagag tcttaaaaa cgcaataac ccaggagcg 
      cagacgtta cggatttgt tcttgaaag gcagtgctc 
      tctggcgaa acattattt atcttttca caatcacgg 
      actggctgc ataccaaca aaatctgga gaaatggtc 
      ggaagttgc caaaacaag ggaatatct tccggaatc 
      tgtcgaaaa cattttcac aaacagact aaggcaaat 
      atcagtctg gcaggacga ggacgtgta ctcgaaccc 
      aaggagtcc taatctcga agacgatcg aatcagtat 
      tagctctca gttacggaa aaccaagag catggacgc 
      gtcctgtct tgatatacg ttcaatcgg cgacgtcgg 
      gtccccagt gacgacgct atcaagggc caaatcgga 
      gtataattt aaattcttc gacacattc acttgcaca 
      cgagggcca cactccagc ccatcggcc caaatcgat 
      cgctgtccc accgccacc gcagtggga aacgcaact 
      agctcgagc gctttatgc ctacctttc cagtggaga 
      taatcaact cagttttct tgaaggaga tctcatcgc 
      tctgatagg ggagcgaaa taagggctg gcaattcgg 
      agagaactt gaggacaca atgcagtgg atggttccc 
      attagcata cactgaact tctagatga tcctaccac 
      gtcgccaac tcatcgcag tgatgccgg tggatctgt 
      tcaaagtca gcagacgcc aacaaacgg cggcaaccc 
      catgtcgct cggcggctc cggctctcc ttcgggtaa 
      caattcgac gctagaccc tccagcccc tcgtatgtt 
      cggcgacac cctccattt gcatagatc ctccaataa 
      cgctaaaca gattcggcg agcgatcgg ttctaacaa 
      cattccgcc cccggcttt gccggctcc ggtgccgac 
      cccctcctt gccattcca gaagatcgg catttcaca 
      atcacaaag tgccaactt caagtccag cgccgacac 
      gtctagctt ccaatcccc gtaccaaac agggataaa 
      atcatcaac atccacctt tagtttctc gacacctcc 
      cacaggaac ctactccac gcaccccca gcccgcccg 
      ggccgaccg gcccacggc gggcgctcc tctgccgct 
      gcatctgca aagcaagag cggaaaaat cggcggtcc 
      cgtcggaaa cgtatcgtt acacagcag taacgattc 
      cgggttcag caacgatcc gccgccaca accagagat 
      tgattattc agatgacga ctccatttc tggacaaac 
      taaattgcc ggcgagaag agtccgtcg ccagccaaa 
      cagtgacgg taataataa cacagtgca aaagaatgt 
      aagacaggc aaacagtca cgggaattt gatcgacaa 
      cagatatta catgtccga tgatcaaga tgcgactag 
      agagcgtaa aggtctagt taagagaac taagtcgtt 
      ctggaagtt tggcaaaaa cagttcgga taatgagat 
      tttagaagg catggcctt gtggaggca tagggacct 
      tgtggacgt ggaagaaga gaaaattaa acgaaagaa 
      caggtttaa tagtcaaga gagaattaa aaaacctag 
      tcgtgacag atcaaacga ttcagatcg aacaattaa 
      cgccaacca aaatataga aagtgagaa taaaaagga 
      agttgagca gagaaaaaa cgtagaaaa cgcgagaca 
      atcctttag ggaaaaacc aagacatcc tgacttgga 
      caaggaaaa ttacggtta ccacaagcg aaataataa 
      accggcaaa tgaccagtt ttacgacga tgatggtga 
      tggactgat tatgaaaac agttaatcg caagaacat 
      tttgcaaca gtacaataa cgactcctc cgtgggcga 
      ttccgattc cgattcgga aatcacaag cgacgatcc 
      gtacgactg tattctggt ggacgacca gaacgtaaa 
      caagcgcga aaaccactt tcccaacgt tgtggaaat 
      cggcaagaa gctggaaaa gctgtcaaa atcgagcaa 
      gttctcgcc caacaacgg caacaaccg ccattcagt 
      ggcggagaa aaatttgcg caacaattt cgaaaagaa 
      tctcacctc ggacaacca caacctgat ccagtacga 
      gaagcagca cagcttcaa aactttcgg agtggaatc 
      tccggagcc ggacgacaa cgagcgcta tcagagtcg 
      caataaaag aaataatga aactaatgt ccaggaaaa 
      acgccgata ttatgccga tgcgcccaa acgcaataa 
      ctcaaacga aaaacaaac tagaccgag ttacgaaag 
      tatcgattc ggacgtgga caagcggcg ggagggccg 
      cagcagccg ccgatcgaa ttcggaaac ggagccccg 
      agataaaaa atattacga ctcggccaa tgacgagtt 
      gtcggacgc cgcggaaaa caggcagat tattccgcg 
      gacgaagct gactaagag caatagtca ccactcgga 
      ggagaacgg gctcatggg gtacgggga gacgctgca 
      gaggcgctt gaaaaagct ggaatacgg gaccaagta 
      cgacgaaaa gtcggcaca taacgggaa catgtacgg 
      gccttggta tgacttgtg gggccttga cgcttccgc 
      ccgaaaata actttctga ctttcttga atttttgca 
      tgtctttga tcattgcac ggtttacgg ttttggctc 
      tttttcagg tctaataac tttgccacc attaagctg 
      cgcaaaact aatcctaac acagaccga aatgcttca 
      cttatcctt gagtagatt ggcttcact actgttttc 
      gttcttcac caacatacg ctagtctta attattact 
      ctatacttg catgcgtta tacgggacg ttgttattg 
      tacatatta tatttattg ttgtagcga tactcttat 
      tttcactca ttttttgta catatctac ttttggtgg 
      ttggggtgg tgcaataag gtttgaatg taatacaaa 
      ctaactgta tttgcttcg taatttaaa tctatctat 
      tgttaagtt gaataccgc cagctcaat cccttttaa 
      tatgaaaca tttttaggg gctagtgaa atcagagac 
      tgtgataaa cttacaact tttgtagtg tattatact 
      aacgcattt agtaagatt gaaaaataa ttagcaaaa 
      cacgtaatc atacactaa cgaatactc aataggtac 
      atacatgtt atattgcca ctttttgaa taattataa 
      tctttgacg aatgtttta ttgttgctg accgtccat 
      tttgttaca tttagacat aatgttagt gttatagtg 
      tggtaaatt aattgtatt aatatcatt aattattat 
      tacctcgga atatataaa ggcaggggt tccctaggc 
      tagaacttg aatgtaaca gtccactta accgtatat 
      ttattagaa taaataata tactcgtag ctattgtct 
      attgtaaac ctgacaaac aaataactt ggttttaca 
      taaaaacag cagagttat tattgttgt acttaaaat 
      gtatatcat tttcatata aataaagat ttatattgt 
      tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY