Status
Please wait ...
Gene information
General information
TC002895 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 7034208..7042333
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002895
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03463

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002895 RA
      MAQIYSKLP LPVTGTIKT TCVDYVHPW TDSCLQGIC 
      GLYLYAIFD SLRIYGTVY VLTLLMKGR IPTKEDLKK 
      TVLGILQST AFLSGTGFG YSLFLCVLR RLLGNFNIL 
      TVSAIPAFL SSVFSILIE RPSRRTLLC LYVSNVATE 
      TLWNMALSR KMVRNVKYG DTAIFAVGM AVLLMYYKG 
      GYHKDSEGK ADSMFGVLR FVVGPYEEK DYSARSSQA 
      STFYRQQVQ NIPNVGSRA KSGSWHSSS RSKNPVFHV 
      ITQALRVYK KIIYKIKCC DRHLACPHP FSCLYYTMQ 
      GVGKMFSIG LGIQVTLKL VLNIKKVVQ SPRNLKAIL 
      FRKDTLNLA VFLGGFSGL FRAVLCILR RATGKDSPS 
      YAIPAGLIA GTAFTRYPD TTVSLYVMW KLAQITYIM 
      GINKGIVPR VPGFTEFLY CFSTAVLFH AALLEPTNL 
      RPSYWKFLH SISGGRIAC MNRIPLDAW GLGTSESLE 
      RVLELTKTQ RVLK
    CDS sequence
    • >TC002895 RA
      atggcccag atttacagt aaattgcct ctccctgtg 
      acagggacc ataaaaact acgtgtgtg gactatgtt 
      catccctgg acggacagt tgtttgcaa gggatttgt 
      gggctgtat ttgtatgca atttttgat tccttgagg 
      atatatggg acggtttat gtgttgact ctgttgatg 
      aaaggccgt attccgaca aaggaagat ttgaaaaaa 
      acagtatta ggaatttta cagtcgacc gcttttctc 
      agcggtact ggatttggg tactctcta tttttatgc 
      gttttaagg cggcttttg ggcaacttt aacatcttg 
      acagtgtcg gcaatccca gcattctta tcaagcgta 
      ttttcgatt ctaattgaa aggccttcc cgacgcact 
      ttgttgtgc ctatacgtc agtaatgtc gccacagaa 
      accctctgg aacatggcc ttatcgcgc aaaatggtc 
      cggaacgta aaatacggc gatacggcc atttttgcc 
      gtcggaatg gcagtttta ctcatgtat tataaaggg 
      gggtaccat aaagactca gaggggaag gctgacagc 
      atgtttgga gtcttaagg tttgtagtg gggccttat 
      gaagaaaaa gactacagt gcgaggtcg agccaagcc 
      agcacattt tacagacag caagtccaa aacattcca 
      aatgttggc tcgagggcg aagtctgga agttggcat 
      tccagctct cgatcaaaa aaccccgtt tttcacgtc 
      attactcaa gctctccgt gtttataaa aaaatcata 
      tacaaaatc aagtgctgt gatagacac ctagcgtgt 
      ccgcatccg ttcagttgt ttgtattat acgatgcaa 
      ggcgtaggg aaaatgttt agcatcggt ttagggata 
      caagtgact ttaaagcta gttcttaat ataaagaaa 
      gtcgtccag agcccgaga aatttgaaa gcgatctta 
      tttaggaaa gatactttg aacctggcg gtgttcctt 
      ggaggtttc tcgggttta tttagagcc gttttgtgc 
      attttgagg agggccaca ggcaaggat agcccctct 
      tatgccatc ccagctggt ttgattgct ggaactgcc 
      tttacgcga tatccagac accacagta tcactctat 
      gtcatgtgg aaattggcc caaataact tacatcatg 
      ggtatcaac aaaggaatc gtgcctcga gtcccgggc 
      ttcaccgaa tttttgtat tgcttcagt actgccgtt 
      cttttccac gcagctctg ctggaaccg acaaatttg 
      aggcctagt tattggaaa tttttacac tcgatttcc 
      ggcggaaga atcgcttgt atgaaccgg attccgttg 
      gatgcttgg gggctcggt acttctgaa tctctagaa 
      cgtgttcta gaactaact aaaacacaa agagtcctg 
      aaatag
    mRNA sequence
    • >TC002895 RA
      tgaaagtgg aagttgttt gtagtgttg cgacagcaa 
      agattaaac tgtcgatgt tttattttt gtcagttcg 
      gctgtgctg tgacgtaaa atgtgttct tatttacat 
      atctcctgc aacaataag tttgtgata agctgaaga 
      acggccaag attatagat ttttttctc gtattgttg 
      tggttttgg gaaaatggc ccagattta cagtaaatt 
      gcctctccc tgtgacagg gaccataaa aactacgtg 
      tgtggacta tgttcatcc ctggacgga cagttgttt 
      gcaagggat ttgtgggct gtatttgta tgcaatttt 
      tgattcctt gaggatata tgggacggt ttatgtgtt 
      gactctgtt gatgaaagg ccgtattcc gacaaagga 
      agatttgaa aaaaacagt attaggaat tttacagtc 
      gaccgcttt tctcagcgg tactggatt tgggtactc 
      tctattttt atgcgtttt aaggcggct tttgggcaa 
      ctttaacat cttgacagt gtcggcaat cccagcatt 
      cttatcaag cgtattttc gattctaat tgaaaggcc 
      ttcccgacg cactttgtt gtgcctata cgtcagtaa 
      tgtcgccac agaaaccct ctggaacat ggccttatc 
      gcgcaaaat ggtccggaa cgtaaaata cggcgatac 
      ggccatttt tgccgtcgg aatggcagt tttactcat 
      gtattataa aggggggta ccataaaga ctcagaggg 
      gaaggctga cagcatgtt tggagtctt aaggtttgt 
      agtggggcc ttatgaaga aaaagacta cagtgcgag 
      gtcgagcca agccagcac attttacag acagcaagt 
      ccaaaacat tccaaatgt tggctcgag ggcgaagtc 
      tggaagttg gcattccag ctctcgatc aaaaaaccc 
      cgtttttca cgtcattac tcaagctct ccgtgttta 
      taaaaaaat catatacaa aatcaagtg ctgtgatag 
      acacctagc gtgtccgca tccgttcag ttgtttgta 
      ttatacgat gcaaggcgt agggaaaat gtttagcat 
      cggtttagg gatacaagt gactttaaa gctagttct 
      taatataaa gaaagtcgt ccagagccc gagaaattt 
      gaaagcgat cttatttag gaaagatac tttgaacct 
      ggcggtgtt ccttggagg tttctcggg tttatttag 
      agccgtttt gtgcatttt gaggagggc cacaggcaa 
      ggatagccc ctcttatgc catcccagc tggtttgat 
      tgctggaac tgcctttac gcgatatcc agacaccac 
      agtatcact ctatgtcat gtggaaatt ggcccaaat 
      aacttacat catgggtat caacaaagg aatcgtgcc 
      tcgagtccc gggcttcac cgaattttt gtattgctt 
      cagtactgc cgttctttt ccacgcagc tctgctgga 
      accgacaaa tttgaggcc tagttattg gaaattttt 
      acactcgat ttccggcgg aagaatcgc ttgtatgaa 
      ccggattcc gttggatgc ttgggggct cggtacttc 
      tgaatctct agaacgtgt tctagaact aactaaaac 
      acaaagagt cctgaaata gtcgaacta ggtcacgtg 
      gtacttttg gaactagtc aaagtgggt gttagtttg 
      caaaagttt cgagaatac ttactttaa cgattaggg 
      aatttttga cgctggttg gttttttat tgagggtag 
      cacaactct tttgttatt ttcttttca tgtttttaa 
      taaatgaga tttttattg caatatttt aatgaaata 
      aagttaaac aaaccaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY