| General information | |||
| TC032415
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 7293316..7415887 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC032415 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03462 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Days post injection
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence -
Penetrance (percentage of animals/eggs on slide): 50% - 80%
larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva appendages rounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs segment potentiallyfused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva appendages rounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs segment potentiallyfused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
Days post injection
- >TC032415 RA
MEVICVLIL VWSTLTSNA LPPVIRIGA IFTEDQKDS SVELAFKYA VYKINKDRV LLSNTTLVY DIQYVPRDD SFRTSKKVC RQMEFGVQA IFGPSDPIL GAHIQSICE ALDVPHLEA RIDFEPLSK DLSINLHPS QEHMNKAFK DLMTFLNWT KVAIIYEED YGLFKLQEL VKAPAAART EMYIRQAGP TSYRQVLKE VRQKEIYKL IVDTNPRNI QKFFRAILQ LQMNDYRYH YMFTTFDLE TFDLEDFKY NSVNITAFR IVDVDDPQV KESLEVMEK FQPIGHAIL NKSGIIQAE PALMFDSVY VFAKGLAAM GSIKPMNLS CDVEKPWDD GSSLYNYLG DVDDLRGLT GNIEFNGGK RSNFKLDLL KLKKEEIRK VGQWTPSGG VNITDPNAF YESHAPNIT LVVMTREER PYVMVKDEK NLTGNARYE GFCIDLLKW IAGQVGFQY TIRLVPDHM YGVYDPDTK EWNGIVREL MEKRADLAV ASMTINYAR ESVIDFTKP FMNLGIGIL FKVPTSQPT RLFSFMNPL AVEIWLYVL AAYMLVSFT LFVMARFSP YEWNNPHPC HQESDIVEN QFSVSNSFW FITGTFLRQ GSGLNPKQS SKSEPSRLF SFLNPLAMN IWLYMAGAY VLVSITIWI VARFSPLEW KEPELCDDC LLKKYSILF EFDEHCEHD ASSGIGSDA ADDLASLPN NDNDIDEVI ESINDPCLS HEHADGRTL EILENGFTI GNSFWFAIG SLMQQGSDL NPKATSTRI VGGIWWFFT LIIISSYTA NLAAFLTVE RMITPIESA QDLADQTDI AYGTLEGGS TMTFFRDSK IGIYQKMWR FMESRKPSV FVKTYEEGV QRVLEGNYA FLMESTMLD YAVQRDCNL TQIGGLLDS KGYGIATPK GSPWRDKIS LAILELQEK GVIQILYDK WWKNTGDVC NRDDKSKES KANALGVEN IGGVFVVLL CGLALAILV AILEFCWNS KKNAQTDRQ SLCSEMAEE LRFAVRCHG SRQRPALRR SCTRCSPAT TYVPAALDL PHINGEGVM LPMMDLKKS PISYDVDT
- >TC032415 RB
MEVICVLIL VWSTLTSNA LPPVIRIGA IFTEDQKDS SVELAFKYA VYKINKDRV LLSNTTLVY DIQYVPRDD SFRTSKKVC RQMEFGVQA IFGPSDPIL GAHIQSICE ALDVPHLEA RIDFEPLSK DLSINLHPS QEHMNKAFK DLMTFLNWT KVAIIYEED YGLFKLQEL VKAPAAART EMYIRQAGP TSYRQVLKE VRQKEIYKL IVDTNPRNI QKFFRAILQ LQMNDYRYH YMFTTFDLE TFDLEDFKY NSVNITAFR IVDVDDPQV KESLEVMEK FQPIGHAIL NKSGIIQAE PALMFDSVY VFAKGLAAM GSIKPMNLS CDVEKPWDD GSSLYNYLG DVDDLRGLT GNIEFNGGK RSNFKLDLL KLKKEEIRK VGQWTPSGG VNITDPNAF YESHAPNIT LVVMTREER PYVMVKDEK NLTGNARYE GFCIDLLKW IAGQVGFQY TIRLVPDHM YGVYDPDTK EWNGIVREL MEKRADLAV ASMTINYAR ESVIDFTKP FMNLGIGIL FKVPTSQPT RLFSFMNPL AVEIWLYVL AAYMLVSFT LFVMARFSP YEWNNPHPC HQESDIVEN QFSVSNSFW FITGTFLRQ GSGLNPKQS SKSEPSRLF SFLNPLAMN IWLYMAGAY VLVSITIWI VARFSPLEW KEPELCDDC LLKKYSILF EFDEHCEHD ASSGIGSDA ADDLASLPN NDNDIDEVI ESINDPCLS HEHADGRTL EILENGFTI GNSFWFAIG SLMQQGSDL NPKATSTRI VGGIWWFFT LIIISSYTA NLAAFLTVE RMITPIESA QDLADQTDI AYGTLEGGS TMTFFRDSK IGIYQKMWR FMESRKPSV FVKTYEEGV QRVLEGNYA FLMESTMLD YAVQRDCNL TQIGGLLDS KGYGIATPK GSPWRDKIS LAILELQEK GVIQILYDK WWKNTGDVC NRDDKSKES KANALGVEN IGGVFVVLL CGLALAILV AILEFCWNS KKNAQTDRQ SLCSEMAEE LRFAVRCHG SRQRPALRR SCTRCSPAT TYVPAALDL PHINGPMNL MEIQFIPES SQHAQRRDA AHDGPEEVA HQLRRRHLA ATLRWGHVD VRVHLQRHP GLLRGPVQL PPAAGGHPQ LQGHSAVPR SGEKKCHAG T
- >TC032415 RA
atggaggtg atatgtgtg ctgatactc gtttggagt acgctgacc tcgaacgcg ctgccacct gtcattcgc attggtgcg attttcacg gaagaccag aaagatagc agcgtcgaa ttagcattc aaatatgcg gtctacaaa attaacaaa gatagggtt ttgctttca aacaccact ctagtatac gatatccaa tacgtgccc cgggacgac agcttccga acgtcgaaa aaagtgtgt cgtcagatg gaattcggc gtccaggcc atcttcggg ccctcggat ccaatatta ggagcacat attcaaagt atttgcgaa gctctagac gtgccccat ctcgaggct cgaatagac ttcgagccc ctgtccaag gacttgtca atcaattta cacccgagt caagagcac atgaacaag gcattcaaa gacctgatg accttcctc aactggacc aaagtcgcc attatatac gaggaagac tacggcctt ttcaagctg caagaattg gtcaaggcg ccggctgct gcaaggact gaaatgtat atccgccag gccggcccc acttcatat cgacaagtg ctgaaagaa gtgagacaa aaagaaata tacaagctc atcgtcgac acgaatccc agaaacatc caaaaattt tttagagcg attttgcaa ctgcagatg aacgactat cgctaccac tacatgttc accaccttt gatttagag acattcgat ctggaagat ttcaaatac aattctgta aatattacg gcattcaga attgttgat gtggatgat ccccaagta aaagaatcc cttgaagtt atggaaaaa ttccagccg attggccat gcaatcctt aataaaagc gggattatt caggcggag cctgcgcta atgtttgac tcagtgtat gtttttgct aaagggctg gctgccatg ggttctata aagccaatg aacttatct tgcgacgtc gagaaaccc tgggatgat ggatcgtcc ctgtacaat tatttgggc gacgtggat gatctccga ggtttgaca gggaacata gaatttaat ggtggcaag aggtcaaac ttcaaactg gacctgctc aagctgaag aaagaggag atccggaaa gtgggccag tggacgccg tcggggggc gttaacatt actgacccc aacgctttc tacgaaagt cacgcgccg aacataacc cttgtcgtc atgactagg gaggagcgt ccgtacgtg atggtgaaa gatgaaaaa aatttaacc ggaaacgcc agatacgag ggcttttgc atcgaccta ttgaaatgg atcgcaggt caggtcggc ttccagtac acaatccgt ctggtcccc gatcacatg tatggagtg tacgacccc gataccaaa gaatggaac ggcatcgtg cgcgagctt atggaaaaa cgggcggac ctcgctgtg gcttccatg acaataaat tatgcacgg gagagcgta atcgacttt actaaaccg tttatgaac ctcggcata ggaattcta ttcaaggtt cccaccagc caacccacg cgcctgttc agttttatg aacccctta gcggtggaa atttggctt tacgtttta gcggcgtat atgctggtg tcatttaca ttgttcgta atggcgaga ttttcgccg tacgagtgg aacaacccc cacccctgt caccaggaa tccgacatt gtcgagaac caattctcg gtttccaat agtttttgg tttataacg ggaacattt ttacgacaa ggatccggt ttaaatcct aagcagtcg tcgaaaagc gagccgagt cggctgttt tcctttctc aaccctttg gcgatgaac atatggctc tacatggcc ggggcttac gtcctggta tcgataaca atttggata gtggcccgg ttttcgccc cttgagtgg aaagagccg gagctgtgc gacgactgt ctcctaaaa aaatattca atcttgttc gagtttgac gaacattgc gagcacgac gcatcatca ggcatcggc agcgacgcc gctgacgat ttagcctcg cttcctaac aacgacaac gatattgac gaagtaatc gaatccatt aatgatccg tgtctaagt cacgaacac gccgacgga agaacatta gaaatactc gagaacgga ttcacgata ggcaacagc ttctggttt gccatcggc agtttgatg cagcaagga tcagatctc aacccgaag gcaacgtct acgaggatc gtaggcgga atctggtgg ttttttact ctcataatc atctcttcc tacaccgca aatttggcc gccttccta acggtggaa cgcatgatt acccccatt gagagtgcc caagacctg gccgatcag accgatatt gcttatgga actctcgaa ggcggcagc actatgacg ttttttagg gattcgaag ataggcatt taccaaaaa atgtggcgg tttatggag agccgaaag ccgtcggtg ttcgtgaaa acgtacgag gaaggtgtg caacgcgtg ctggaggga aattacgcc ttcctaatg gaatcaact atgctggac tacgccgtt caaagagat tgcaattta acgcaaatt ggaggcctg ctggactca aaaggctac gggatagcg acgccgaaa ggcagtccg tggagagac aaaatatcg cttgcaatc ctcgagctt caagagaaa ggagtgatc caaatactc tacgacaag tggtggaaa aatactgga gatgtttgt aatcgagac gataaaagt aaagaatcc aaagccaac gccttgggg gtcgaaaat attgggggt gtgtttgtg gtgcttctc tgcggcctg gccctcgcc attctagtc gccatcctc gagttctgc tggaacagc aagaaaaac gcccagacc gaccgccag tcgctgtgt tcggaaatg gcagaagaa ttgcggttt gcggtgcgt tgtcacggc tccagacag cgtcccgct ctgcgaaga tcttgtaca aggtgtagt ccggccacg acatatgtg cctgcggcc cttgattta ccgcacatc aatggggaa ggcgtgatg ctgcccatg atggacctg aagaagtcg cccatcagc tacgacgtc gacacttag
- >TC032415 RB
atggaggtg atatgtgtg ctgatactc gtttggagt acgctgacc tcgaacgcg ctgccacct gtcattcgc attggtgcg attttcacg gaagaccag aaagatagc agcgtcgaa ttagcattc aaatatgcg gtctacaaa attaacaaa gatagggtt ttgctttca aacaccact ctagtatac gatatccaa tacgtgccc cgggacgac agcttccga acgtcgaaa aaagtgtgt cgtcagatg gaattcggc gtccaggcc atcttcggg ccctcggat ccaatatta ggagcacat attcaaagt atttgcgaa gctctagac gtgccccat ctcgaggct cgaatagac ttcgagccc ctgtccaag gacttgtca atcaattta cacccgagt caagagcac atgaacaag gcattcaaa gacctgatg accttcctc aactggacc aaagtcgcc attatatac gaggaagac tacggcctt ttcaagctg caagaattg gtcaaggcg ccggctgct gcaaggact gaaatgtat atccgccag gccggcccc acttcatat cgacaagtg ctgaaagaa gtgagacaa aaagaaata tacaagctc atcgtcgac acgaatccc agaaacatc caaaaattt tttagagcg attttgcaa ctgcagatg aacgactat cgctaccac tacatgttc accaccttt gatttagag acattcgat ctggaagat ttcaaatac aattctgta aatattacg gcattcaga attgttgat gtggatgat ccccaagta aaagaatcc cttgaagtt atggaaaaa ttccagccg attggccat gcaatcctt aataaaagc gggattatt caggcggag cctgcgcta atgtttgac tcagtgtat gtttttgct aaagggctg gctgccatg ggttctata aagccaatg aacttatct tgcgacgtc gagaaaccc tgggatgat ggatcgtcc ctgtacaat tatttgggc gacgtggat gatctccga ggtttgaca gggaacata gaatttaat ggtggcaag aggtcaaac ttcaaactg gacctgctc aagctgaag aaagaggag atccggaaa gtgggccag tggacgccg tcggggggc gttaacatt actgacccc aacgctttc tacgaaagt cacgcgccg aacataacc cttgtcgtc atgactagg gaggagcgt ccgtacgtg atggtgaaa gatgaaaaa aatttaacc ggaaacgcc agatacgag ggcttttgc atcgaccta ttgaaatgg atcgcaggt caggtcggc ttccagtac acaatccgt ctggtcccc gatcacatg tatggagtg tacgacccc gataccaaa gaatggaac ggcatcgtg cgcgagctt atggaaaaa cgggcggac ctcgctgtg gcttccatg acaataaat tatgcacgg gagagcgta atcgacttt actaaaccg tttatgaac ctcggcata ggaattcta ttcaaggtt cccaccagc caacccacg cgcctgttc agttttatg aacccctta gcggtggaa atttggctt tacgtttta gcggcgtat atgctggtg tcatttaca ttgttcgta atggcgaga ttttcgccg tacgagtgg aacaacccc cacccctgt caccaggaa tccgacatt gtcgagaac caattctcg gtttccaat agtttttgg tttataacg ggaacattt ttacgacaa ggatccggt ttaaatcct aagcagtcg tcgaaaagc gagccgagt cggctgttt tcctttctc aaccctttg gcgatgaac atatggctc tacatggcc ggggcttac gtcctggta tcgataaca atttggata gtggcccgg ttttcgccc cttgagtgg aaagagccg gagctgtgc gacgactgt ctcctaaaa aaatattca atcttgttc gagtttgac gaacattgc gagcacgac gcatcatca ggcatcggc agcgacgcc gctgacgat ttagcctcg cttcctaac aacgacaac gatattgac gaagtaatc gaatccatt aatgatccg tgtctaagt cacgaacac gccgacgga agaacatta gaaatactc gagaacgga ttcacgata ggcaacagc ttctggttt gccatcggc agtttgatg cagcaagga tcagatctc aacccgaag gcaacgtct acgaggatc gtaggcgga atctggtgg ttttttact ctcataatc atctcttcc tacaccgca aatttggcc gccttccta acggtggaa cgcatgatt acccccatt gagagtgcc caagacctg gccgatcag accgatatt gcttatgga actctcgaa ggcggcagc actatgacg ttttttagg gattcgaag ataggcatt taccaaaaa atgtggcgg tttatggag agccgaaag ccgtcggtg ttcgtgaaa acgtacgag gaaggtgtg caacgcgtg ctggaggga aattacgcc ttcctaatg gaatcaact atgctggac tacgccgtt caaagagat tgcaattta acgcaaatt ggaggcctg ctggactca aaaggctac gggatagcg acgccgaaa ggcagtccg tggagagac aaaatatcg cttgcaatc ctcgagctt caagagaaa ggagtgatc caaatactc tacgacaag tggtggaaa aatactgga gatgtttgt aatcgagac gataaaagt aaagaatcc aaagccaac gccttgggg gtcgaaaat attgggggt gtgtttgtg gtgcttctc tgcggcctg gccctcgcc attctagtc gccatcctc gagttctgc tggaacagc aagaaaaac gcccagacc gaccgccag tcgctgtgt tcggaaatg gcagaagaa ttgcggttt gcggtgcgt tgtcacggc tccagacag cgtcccgct ctgcgaaga tcttgtaca aggtgtagt ccggccacg acatatgtg cctgcggcc cttgattta ccgcacatc aatgggcca atgaattta atggaaatc caatttatt cccgaatcg agtcaacac gcgcaaagg cgtgatgct gcccatgat ggacctgaa gaagtcgcc catcagcta cgacgtcga cacttagcg gcgactcta cgctggggg cacgtcgac gttcgcgtg cacctccag cgccatccg ggactgctg cgggggccc gtccaactc ccgcccgca gcgggcggc caccctcaa ttacagggc cactccgca gtgcccaga tctggcgaa aaaaagtgc cacgctggg acgtaa
- >TC032415 RA
aatcacacg ccgctccgg tagttcaga acgatcaag cgcgtgctc atgatacga tcaccataa tcagaaaaa atatttttc gagcagtgc gtaatctta ttgtgaaat ctgaccaaa aggacacaa gtcgggtaa attggttct gctgtcgtg ggtggcaat cattaaaag ttccgtctc tggtttttc ttttattac cgttcgatt gctagcaat tcttgtaag ttattttat ttgttgtaa caaacgtta tttcaggtt tcaattcca tttcgttgc gaaatttaa tttagataa gatctttat tgctatcta acatttcag cttcgaaag gtccgttta aactatata aataaaatc agtgtagtg taataatga attagataa actcgactc aaagtgttc aaatatttg tggaccgga ccctcccta aatcaatgt ttatctaag tgtattcta attataaac attgttgat ggtattaat ttagtgtgg tgaatttgg atgtgatgc tgcattgca tatgcagtg atatctggt catgctgat tggtcgatc agtgatagc aaaatggag gtgatatgt gtgctgata ctcgtttgg agtacgctg acctcgaac gcgctgcca cctgtcatt cgcattggt gcgattttc acggaagac cagaaagat agcagcgtc gaattagca ttcaaatat gcggtctac aaaattaac aaagatagg gttttgctt tcaaacacc actctagta tacgatatc caatacgtg ccccgggac gacagcttc cgaacgtcg aaaaaagtg tgtcgtcag atggaattc ggcgtccag gccatcttc gggccctcg gatccaata ttaggagca catattcaa agtatttgc gaagctcta gacgtgccc catctcgag gctcgaata gacttcgag cccctgtcc aaggacttg tcaatcaat ttacacccg agtcaagag cacatgaac aaggcattc aaagacctg atgaccttc ctcaactgg accaaagtc gccattata tacgaggaa gactacggc cttttcaag ctgcaagaa ttggtcaag gcgccggct gctgcaagg actgaaatg tatatccgc caggccggc cccacttca tatcgacaa gtgctgaaa gaagtgaga caaaaagaa atatacaag ctcatcgtc gacacgaat cccagaaac atccaaaaa ttttttaga gcgattttg caactgcag atgaacgac tatcgctac cactacatg ttcaccacc tttgattta gagacattc gatctggaa gatttcaaa tacaattct gtaaatatt acggcattc agaattgtt gatgtggat gatccccaa gtaaaagaa tcccttgaa gttatggaa aaattccag ccgattggc catgcaatc cttaataaa agcgggatt attcaggcg gagcctgcg ctaatgttt gactcagtg tatgttttt gctaaaggg ctggctgcc atgggttct ataaagcca atgaactta tcttgcgac gtcgagaaa ccctgggat gatggatcg tccctgtac aattatttg ggcgacgtg gatgatctc cgaggtttg acagggaac atagaattt aatggtggc aagaggtca aacttcaaa ctggacctg ctcaagctg aagaaagag gagatccgg aaagtgggc cagtggacg ccgtcgggg ggcgttaac attactgac cccaacgct ttctacgaa agtcacgcg ccgaacata acccttgtc gtcatgact agggaggag cgtccgtac gtgatggtg aaagatgaa aaaaattta accggaaac gccagatac gagggcttt tgcatcgac ctattgaaa tggatcgca ggtcaggtc ggcttccag tacacaatc cgtctggtc cccgatcac atgtatgga gtgtacgac cccgatacc aaagaatgg aacggcatc gtgcgcgag cttatggaa aaacgggcg gacctcgct gtggcttcc atgacaata aattatgca cgggagagc gtaatcgac tttactaaa ccgtttatg aacctcggc ataggaatt ctattcaag gttcccacc agccaaccc acgcgcctg ttcagtttt atgaacccc ttagcggtg gaaatttgg ctttacgtt ttagcggcg tatatgctg gtgtcattt acattgttc gtaatggcg agattttcg ccgtacgag tggaacaac ccccacccc tgtcaccag gaatccgac attgtcgag aaccaattc tcggtttcc aatagtttt tggtttata acgggaaca tttttacga caaggatcc ggtttaaat cctaagcag tcgtcgaaa agcgagccg agtcggctg ttttccttt ctcaaccct ttggcgatg aacatatgg ctctacatg gccggggct tacgtcctg gtatcgata acaatttgg atagtggcc cggttttcg ccccttgag tggaaagag ccggagctg tgcgacgac tgtctccta aaaaaatat tcaatcttg ttcgagttt gacgaacat tgcgagcac gacgcatca tcaggcatc ggcagcgac gccgctgac gatttagcc tcgcttcct aacaacgac aacgatatt gacgaagta atcgaatcc attaatgat ccgtgtcta agtcacgaa cacgccgac ggaagaaca ttagaaata ctcgagaac ggattcacg ataggcaac agcttctgg tttgccatc ggcagtttg atgcagcaa ggatcagat ctcaacccg aaggcaacg tctacgagg atcgtaggc ggaatctgg tggtttttt actctcata atcatctct tcctacacc gcaaatttg gccgccttc ctaacggtg gaacgcatg attaccccc attgagagt gcccaagac ctggccgat cagaccgat attgcttat ggaactctc gaaggcggc agcactatg acgtttttt agggattcg aagataggc atttaccaa aaaatgtgg cggtttatg gagagccga aagccgtcg gtgttcgtg aaaacgtac gaggaaggt gtgcaacgc gtgctggag ggaaattac gccttccta atggaatca actatgctg gactacgcc gttcaaaga gattgcaat ttaacgcaa attggaggc ctgctggac tcaaaaggc tacgggata gcgacgccg aaaggcagt ccgtggaga gacaaaata tcgcttgca atcctcgag cttcaagag aaaggagtg atccaaata ctctacgac aagtggtgg aaaaatact ggagatgtt tgtaatcga gacgataaa agtaaagaa tccaaagcc aacgccttg ggggtcgaa aatattggg ggtgtgttt gtggtgctt ctctgcggc ctggccctc gccattcta gtcgccatc ctcgagttc tgctggaac agcaagaaa aacgcccag accgaccgc cagtcgctg tgttcggaa atggcagaa gaattgcgg tttgcggtg cgttgtcac ggctccaga cagcgtccc gctctgcga agatcttgt acaaggtgt agtccggcc acgacatat gtgcctgcg gcccttgat ttaccgcac atcaatggg gaaggcgtg atgctgccc atgatggac ctgaagaag tcgcccatc agctacgac gtcgacact tagcggcga ctctacgct gggggcacg tcgacgttc gcgtgcacc tccagcgcc atccgggac tgctgcggg ggcccgtcc aactcccgc ccgcagcgg gcggccacc ctcaattac agggccact ccgcagtgc ccagatctg gcgaaaaaa agtgccacg ctgggacgt aactccgga ttctgtcgc gatacgtgt gacattgta caaagcggt ctgcagaac gacgtcccg gataaaata ctgtggaag ttcgattgt gatattggt agtccgaac aacccagac gtagtgacc aggacactg cccgacgcc atcgtaagt aggaccacc actttgtga tacataggt agaatttgt aaattgaat caaataaat ggactgatg tttgtacca atttcaata caattgtta gggaattta cgctgattt caatgtttt acaaaaatc gaattttag agactgact aaccagaat tcttaatca aaaataaaa aacgcaaat ccgtctaaa tcttctacc ctcgcccat attttccaa atcgaacct ttcgtcggc ccaaattgc ctcagttcc ttccgaccc aagtcacgt ctaaccaaa ttcgcggtg ttccaacaa agtaaatgg tctataaag attttttag caacgcttt aatttttta tgcctcatc tattcgtaa aactgaact gaaacgaca catcgttta gagccagca atgaactca accataagc tggaaactg gctaaatat tttatttaa ttgatttat tttattccc tcgacggct ccgctttgt ccgactgag actaaatga aagaaatga gaaaaaata aagcggatt gaagaattg aaacttggg agttgttat tcaaacaat cgtttatgt ttcgcaata aaatatcaa cttttaggg gataaaagc ttcggaatt catactttg gctacaata tttttacga tgctttaaa gttgtgagt tttacggcg gcgttcaag taacacatc gtggaattt ttaccatta attttgcac tgtgcaggg gaatatacg taaaattaa aagcatatc ttttagggg aatgcggca gtctctaaa attttggtt tgtttctca aaattggta atgacttca acacagttc attaatttt aaataattt ccgtccatt atatttcat tggtttgac ccgtaatta acgctaaat cagtcggta ttaatgcaa caatcaaaa ttcttgtta aagttttat tactaaaat aaccatcaa tgttatgaa acataataa attggtttg tgttaa
- >TC032415 RB
aatcacacg ccgctccgg tagttcaga acgatcaag cgcgtgctc atgatacga tcaccataa tcagaaaaa atatttttc gagcagtgc gtaatctta ttgtgaaat ctgaccaaa aggacacaa gtcgggtaa attggttct gctgtcgtg ggtggcaat cattaaaag ttccgtctc tggtttttc ttttattac cgttcgatt gctagcaat tcttgtaag ttattttat ttgttgtaa caaacgtta tttcaggtt tcaattcca tttcgttgc gaaatttaa tttagataa gatctttat tgctatcta acatttcag cttcgaaag gtccgttta aactatata aataaaatc agtgtagtg taataatga attagataa actcgactc aaagtgttc aaatatttg tggaccgga ccctcccta aatcaatgt ttatctaag tgtattcta attataaac attgttgat ggtattaat ttagtgtgg tgaatttgg atgtgatgc tgcattgca tatgcagtg atatctggt catgctgat tggtcgatc agtgatagc aaaatggag gtgatatgt gtgctgata ctcgtttgg agtacgctg acctcgaac gcgctgcca cctgtcatt cgcattggt gcgattttc acggaagac cagaaagat agcagcgtc gaattagca ttcaaatat gcggtctac aaaattaac aaagatagg gttttgctt tcaaacacc actctagta tacgatatc caatacgtg ccccgggac gacagcttc cgaacgtcg aaaaaagtg tgtcgtcag atggaattc ggcgtccag gccatcttc gggccctcg gatccaata ttaggagca catattcaa agtatttgc gaagctcta gacgtgccc catctcgag gctcgaata gacttcgag cccctgtcc aaggacttg tcaatcaat ttacacccg agtcaagag cacatgaac aaggcattc aaagacctg atgaccttc ctcaactgg accaaagtc gccattata tacgaggaa gactacggc cttttcaag ctgcaagaa ttggtcaag gcgccggct gctgcaagg actgaaatg tatatccgc caggccggc cccacttca tatcgacaa gtgctgaaa gaagtgaga caaaaagaa atatacaag ctcatcgtc gacacgaat cccagaaac atccaaaaa ttttttaga gcgattttg caactgcag atgaacgac tatcgctac cactacatg ttcaccacc tttgattta gagacattc gatctggaa gatttcaaa tacaattct gtaaatatt acggcattc agaattgtt gatgtggat gatccccaa gtaaaagaa tcccttgaa gttatggaa aaattccag ccgattggc catgcaatc cttaataaa agcgggatt attcaggcg gagcctgcg ctaatgttt gactcagtg tatgttttt gctaaaggg ctggctgcc atgggttct ataaagcca atgaactta tcttgcgac gtcgagaaa ccctgggat gatggatcg tccctgtac aattatttg ggcgacgtg gatgatctc cgaggtttg acagggaac atagaattt aatggtggc aagaggtca aacttcaaa ctggacctg ctcaagctg aagaaagag gagatccgg aaagtgggc cagtggacg ccgtcgggg ggcgttaac attactgac cccaacgct ttctacgaa agtcacgcg ccgaacata acccttgtc gtcatgact agggaggag cgtccgtac gtgatggtg aaagatgaa aaaaattta accggaaac gccagatac gagggcttt tgcatcgac ctattgaaa tggatcgca ggtcaggtc ggcttccag tacacaatc cgtctggtc cccgatcac atgtatgga gtgtacgac cccgatacc aaagaatgg aacggcatc gtgcgcgag cttatggaa aaacgggcg gacctcgct gtggcttcc atgacaata aattatgca cgggagagc gtaatcgac tttactaaa ccgtttatg aacctcggc ataggaatt ctattcaag gttcccacc agccaaccc acgcgcctg ttcagtttt atgaacccc ttagcggtg gaaatttgg ctttacgtt ttagcggcg tatatgctg gtgtcattt acattgttc gtaatggcg agattttcg ccgtacgag tggaacaac ccccacccc tgtcaccag gaatccgac attgtcgag aaccaattc tcggtttcc aatagtttt tggtttata acgggaaca tttttacga caaggatcc ggtttaaat cctaagcag tcgtcgaaa agcgagccg agtcggctg ttttccttt ctcaaccct ttggcgatg aacatatgg ctctacatg gccggggct tacgtcctg gtatcgata acaatttgg atagtggcc cggttttcg ccccttgag tggaaagag ccggagctg tgcgacgac tgtctccta aaaaaatat tcaatcttg ttcgagttt gacgaacat tgcgagcac gacgcatca tcaggcatc ggcagcgac gccgctgac gatttagcc tcgcttcct aacaacgac aacgatatt gacgaagta atcgaatcc attaatgat ccgtgtcta agtcacgaa cacgccgac ggaagaaca ttagaaata ctcgagaac ggattcacg ataggcaac agcttctgg tttgccatc ggcagtttg atgcagcaa ggatcagat ctcaacccg aaggcaacg tctacgagg atcgtaggc ggaatctgg tggtttttt actctcata atcatctct tcctacacc gcaaatttg gccgccttc ctaacggtg gaacgcatg attaccccc attgagagt gcccaagac ctggccgat cagaccgat attgcttat ggaactctc gaaggcggc agcactatg acgtttttt agggattcg aagataggc atttaccaa aaaatgtgg cggtttatg gagagccga aagccgtcg gtgttcgtg aaaacgtac gaggaaggt gtgcaacgc gtgctggag ggaaattac gccttccta atggaatca actatgctg gactacgcc gttcaaaga gattgcaat ttaacgcaa attggaggc ctgctggac tcaaaaggc tacgggata gcgacgccg aaaggcagt ccgtggaga gacaaaata tcgcttgca atcctcgag cttcaagag aaaggagtg atccaaata ctctacgac aagtggtgg aaaaatact ggagatgtt tgtaatcga gacgataaa agtaaagaa tccaaagcc aacgccttg ggggtcgaa aatattggg ggtgtgttt gtggtgctt ctctgcggc ctggccctc gccattcta gtcgccatc ctcgagttc tgctggaac agcaagaaa aacgcccag accgaccgc cagtcgctg tgttcggaa atggcagaa gaattgcgg tttgcggtg cgttgtcac ggctccaga cagcgtccc gctctgcga agatcttgt acaaggtgt agtccggcc acgacatat gtgcctgcg gcccttgat ttaccgcac atcaatggg ccaatgaat ttaatggaa atccaattt attcccgaa tcgagtcaa cacgcgcaa aggcgtgat gctgcccat gatggacct gaagaagtc gcccatcag ctacgacgt cgacactta gcggcgact ctacgctgg gggcacgtc gacgttcgc gtgcacctc cagcgccat ccgggactg ctgcggggg cccgtccaa ctcccgccc gcagcgggc ggccaccct caattacag ggccactcc gcagtgccc agatctggc gaaaaaaag tgccacgct gggacgtaa ctccggatt ctgtcgcga tacgtgtga cattgtaca aagcggtct gcagaacga cgtcccgga taaaatact gtggaagtt cgattgtga tattggtag tccgaacaa cccagacgt agtgaccag gacactgcc cgacgccat cgtaagtag gaccaccac tttgtgata cataggtag aatttgtaa attgaatca aataaatgg actgatgtt tgtaccaat ttcaataca attgttagg gaatttacg ctgatttca atgttttac aaaaatcga attttagag actgactaa ccagaattc ttaatcaaa aataaaaaa cgcaaatcc gtctaaatc ttctaccct cgcccatat tttccaaat cgaaccttt cgtcggccc aaattgcct cagttcctt ccgacccaa gtcacgtct aaccaaatt cgcggtgtt ccaacaaag taaatggtc tataaagat tttttagca acgctttaa ttttttatg cctcatcta ttcgtaaaa ctgaactga aacgacaca tcgtttaga gccagcaat gaactcaac cataagctg gaaactggc taaatattt tatttaatt gatttattt tattccctc gacggctcc gctttgtcc gactgagac taaatgaaa gaaatgaga aaaaataaa gcggattga agaattgaa acttgggag ttgttattc aaacaatcg tttatgttt cgcaataaa atatcaact tttagggga taaaagctt cggaattca tactttggc tacaatatt tttacgatg ctttaaagt tgtgagttt tacggcggc gttcaagta acacatcgt ggaattttt accattaat tttgcactg tgcagggga atatacgta aaattaaaa gcatatctt ttaggggaa tgcggcagt ctctaaaat tttggtttg tttctcaaa attggtaat gacttcaac acagttcat taattttaa ataatttcc gtccattat atttcattg gtttgaccc gtaattaac gctaaatca gtcggtatt aatgcaaca atcaaaatt cttgttaaa gttttatta ctaaaataa ccatcaatg ttatgaaac ataataaat tggtttgtg ttaa
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