Status
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Gene information
General information
TC032415
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002883,TC002882
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 7293316..7415887
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032415
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03462

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva appendages rounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs segment potentiallyfused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032415 RA
      MEVICVLIL VWSTLTSNA LPPVIRIGA IFTEDQKDS 
      SVELAFKYA VYKINKDRV LLSNTTLVY DIQYVPRDD 
      SFRTSKKVC RQMEFGVQA IFGPSDPIL GAHIQSICE 
      ALDVPHLEA RIDFEPLSK DLSINLHPS QEHMNKAFK 
      DLMTFLNWT KVAIIYEED YGLFKLQEL VKAPAAART 
      EMYIRQAGP TSYRQVLKE VRQKEIYKL IVDTNPRNI 
      QKFFRAILQ LQMNDYRYH YMFTTFDLE TFDLEDFKY 
      NSVNITAFR IVDVDDPQV KESLEVMEK FQPIGHAIL 
      NKSGIIQAE PALMFDSVY VFAKGLAAM GSIKPMNLS 
      CDVEKPWDD GSSLYNYLG DVDDLRGLT GNIEFNGGK 
      RSNFKLDLL KLKKEEIRK VGQWTPSGG VNITDPNAF 
      YESHAPNIT LVVMTREER PYVMVKDEK NLTGNARYE 
      GFCIDLLKW IAGQVGFQY TIRLVPDHM YGVYDPDTK 
      EWNGIVREL MEKRADLAV ASMTINYAR ESVIDFTKP 
      FMNLGIGIL FKVPTSQPT RLFSFMNPL AVEIWLYVL 
      AAYMLVSFT LFVMARFSP YEWNNPHPC HQESDIVEN 
      QFSVSNSFW FITGTFLRQ GSGLNPKQS SKSEPSRLF 
      SFLNPLAMN IWLYMAGAY VLVSITIWI VARFSPLEW 
      KEPELCDDC LLKKYSILF EFDEHCEHD ASSGIGSDA 
      ADDLASLPN NDNDIDEVI ESINDPCLS HEHADGRTL 
      EILENGFTI GNSFWFAIG SLMQQGSDL NPKATSTRI 
      VGGIWWFFT LIIISSYTA NLAAFLTVE RMITPIESA 
      QDLADQTDI AYGTLEGGS TMTFFRDSK IGIYQKMWR 
      FMESRKPSV FVKTYEEGV QRVLEGNYA FLMESTMLD 
      YAVQRDCNL TQIGGLLDS KGYGIATPK GSPWRDKIS 
      LAILELQEK GVIQILYDK WWKNTGDVC NRDDKSKES 
      KANALGVEN IGGVFVVLL CGLALAILV AILEFCWNS 
      KKNAQTDRQ SLCSEMAEE LRFAVRCHG SRQRPALRR 
      SCTRCSPAT TYVPAALDL PHINGEGVM LPMMDLKKS 
      PISYDVDT
    • >TC032415 RB
      MEVICVLIL VWSTLTSNA LPPVIRIGA IFTEDQKDS 
      SVELAFKYA VYKINKDRV LLSNTTLVY DIQYVPRDD 
      SFRTSKKVC RQMEFGVQA IFGPSDPIL GAHIQSICE 
      ALDVPHLEA RIDFEPLSK DLSINLHPS QEHMNKAFK 
      DLMTFLNWT KVAIIYEED YGLFKLQEL VKAPAAART 
      EMYIRQAGP TSYRQVLKE VRQKEIYKL IVDTNPRNI 
      QKFFRAILQ LQMNDYRYH YMFTTFDLE TFDLEDFKY 
      NSVNITAFR IVDVDDPQV KESLEVMEK FQPIGHAIL 
      NKSGIIQAE PALMFDSVY VFAKGLAAM GSIKPMNLS 
      CDVEKPWDD GSSLYNYLG DVDDLRGLT GNIEFNGGK 
      RSNFKLDLL KLKKEEIRK VGQWTPSGG VNITDPNAF 
      YESHAPNIT LVVMTREER PYVMVKDEK NLTGNARYE 
      GFCIDLLKW IAGQVGFQY TIRLVPDHM YGVYDPDTK 
      EWNGIVREL MEKRADLAV ASMTINYAR ESVIDFTKP 
      FMNLGIGIL FKVPTSQPT RLFSFMNPL AVEIWLYVL 
      AAYMLVSFT LFVMARFSP YEWNNPHPC HQESDIVEN 
      QFSVSNSFW FITGTFLRQ GSGLNPKQS SKSEPSRLF 
      SFLNPLAMN IWLYMAGAY VLVSITIWI VARFSPLEW 
      KEPELCDDC LLKKYSILF EFDEHCEHD ASSGIGSDA 
      ADDLASLPN NDNDIDEVI ESINDPCLS HEHADGRTL 
      EILENGFTI GNSFWFAIG SLMQQGSDL NPKATSTRI 
      VGGIWWFFT LIIISSYTA NLAAFLTVE RMITPIESA 
      QDLADQTDI AYGTLEGGS TMTFFRDSK IGIYQKMWR 
      FMESRKPSV FVKTYEEGV QRVLEGNYA FLMESTMLD 
      YAVQRDCNL TQIGGLLDS KGYGIATPK GSPWRDKIS 
      LAILELQEK GVIQILYDK WWKNTGDVC NRDDKSKES 
      KANALGVEN IGGVFVVLL CGLALAILV AILEFCWNS 
      KKNAQTDRQ SLCSEMAEE LRFAVRCHG SRQRPALRR 
      SCTRCSPAT TYVPAALDL PHINGPMNL MEIQFIPES 
      SQHAQRRDA AHDGPEEVA HQLRRRHLA ATLRWGHVD 
      VRVHLQRHP GLLRGPVQL PPAAGGHPQ LQGHSAVPR 
      SGEKKCHAG T
    CDS sequence
    • >TC032415 RA
      atggaggtg atatgtgtg ctgatactc gtttggagt 
      acgctgacc tcgaacgcg ctgccacct gtcattcgc 
      attggtgcg attttcacg gaagaccag aaagatagc 
      agcgtcgaa ttagcattc aaatatgcg gtctacaaa 
      attaacaaa gatagggtt ttgctttca aacaccact 
      ctagtatac gatatccaa tacgtgccc cgggacgac 
      agcttccga acgtcgaaa aaagtgtgt cgtcagatg 
      gaattcggc gtccaggcc atcttcggg ccctcggat 
      ccaatatta ggagcacat attcaaagt atttgcgaa 
      gctctagac gtgccccat ctcgaggct cgaatagac 
      ttcgagccc ctgtccaag gacttgtca atcaattta 
      cacccgagt caagagcac atgaacaag gcattcaaa 
      gacctgatg accttcctc aactggacc aaagtcgcc 
      attatatac gaggaagac tacggcctt ttcaagctg 
      caagaattg gtcaaggcg ccggctgct gcaaggact 
      gaaatgtat atccgccag gccggcccc acttcatat 
      cgacaagtg ctgaaagaa gtgagacaa aaagaaata 
      tacaagctc atcgtcgac acgaatccc agaaacatc 
      caaaaattt tttagagcg attttgcaa ctgcagatg 
      aacgactat cgctaccac tacatgttc accaccttt 
      gatttagag acattcgat ctggaagat ttcaaatac 
      aattctgta aatattacg gcattcaga attgttgat 
      gtggatgat ccccaagta aaagaatcc cttgaagtt 
      atggaaaaa ttccagccg attggccat gcaatcctt 
      aataaaagc gggattatt caggcggag cctgcgcta 
      atgtttgac tcagtgtat gtttttgct aaagggctg 
      gctgccatg ggttctata aagccaatg aacttatct 
      tgcgacgtc gagaaaccc tgggatgat ggatcgtcc 
      ctgtacaat tatttgggc gacgtggat gatctccga 
      ggtttgaca gggaacata gaatttaat ggtggcaag 
      aggtcaaac ttcaaactg gacctgctc aagctgaag 
      aaagaggag atccggaaa gtgggccag tggacgccg 
      tcggggggc gttaacatt actgacccc aacgctttc 
      tacgaaagt cacgcgccg aacataacc cttgtcgtc 
      atgactagg gaggagcgt ccgtacgtg atggtgaaa 
      gatgaaaaa aatttaacc ggaaacgcc agatacgag 
      ggcttttgc atcgaccta ttgaaatgg atcgcaggt 
      caggtcggc ttccagtac acaatccgt ctggtcccc 
      gatcacatg tatggagtg tacgacccc gataccaaa 
      gaatggaac ggcatcgtg cgcgagctt atggaaaaa 
      cgggcggac ctcgctgtg gcttccatg acaataaat 
      tatgcacgg gagagcgta atcgacttt actaaaccg 
      tttatgaac ctcggcata ggaattcta ttcaaggtt 
      cccaccagc caacccacg cgcctgttc agttttatg 
      aacccctta gcggtggaa atttggctt tacgtttta 
      gcggcgtat atgctggtg tcatttaca ttgttcgta 
      atggcgaga ttttcgccg tacgagtgg aacaacccc 
      cacccctgt caccaggaa tccgacatt gtcgagaac 
      caattctcg gtttccaat agtttttgg tttataacg 
      ggaacattt ttacgacaa ggatccggt ttaaatcct 
      aagcagtcg tcgaaaagc gagccgagt cggctgttt 
      tcctttctc aaccctttg gcgatgaac atatggctc 
      tacatggcc ggggcttac gtcctggta tcgataaca 
      atttggata gtggcccgg ttttcgccc cttgagtgg 
      aaagagccg gagctgtgc gacgactgt ctcctaaaa 
      aaatattca atcttgttc gagtttgac gaacattgc 
      gagcacgac gcatcatca ggcatcggc agcgacgcc 
      gctgacgat ttagcctcg cttcctaac aacgacaac 
      gatattgac gaagtaatc gaatccatt aatgatccg 
      tgtctaagt cacgaacac gccgacgga agaacatta 
      gaaatactc gagaacgga ttcacgata ggcaacagc 
      ttctggttt gccatcggc agtttgatg cagcaagga 
      tcagatctc aacccgaag gcaacgtct acgaggatc 
      gtaggcgga atctggtgg ttttttact ctcataatc 
      atctcttcc tacaccgca aatttggcc gccttccta 
      acggtggaa cgcatgatt acccccatt gagagtgcc 
      caagacctg gccgatcag accgatatt gcttatgga 
      actctcgaa ggcggcagc actatgacg ttttttagg 
      gattcgaag ataggcatt taccaaaaa atgtggcgg 
      tttatggag agccgaaag ccgtcggtg ttcgtgaaa 
      acgtacgag gaaggtgtg caacgcgtg ctggaggga 
      aattacgcc ttcctaatg gaatcaact atgctggac 
      tacgccgtt caaagagat tgcaattta acgcaaatt 
      ggaggcctg ctggactca aaaggctac gggatagcg 
      acgccgaaa ggcagtccg tggagagac aaaatatcg 
      cttgcaatc ctcgagctt caagagaaa ggagtgatc 
      caaatactc tacgacaag tggtggaaa aatactgga 
      gatgtttgt aatcgagac gataaaagt aaagaatcc 
      aaagccaac gccttgggg gtcgaaaat attgggggt 
      gtgtttgtg gtgcttctc tgcggcctg gccctcgcc 
      attctagtc gccatcctc gagttctgc tggaacagc 
      aagaaaaac gcccagacc gaccgccag tcgctgtgt 
      tcggaaatg gcagaagaa ttgcggttt gcggtgcgt 
      tgtcacggc tccagacag cgtcccgct ctgcgaaga 
      tcttgtaca aggtgtagt ccggccacg acatatgtg 
      cctgcggcc cttgattta ccgcacatc aatggggaa 
      ggcgtgatg ctgcccatg atggacctg aagaagtcg 
      cccatcagc tacgacgtc gacacttag
    • >TC032415 RB
      atggaggtg atatgtgtg ctgatactc gtttggagt 
      acgctgacc tcgaacgcg ctgccacct gtcattcgc 
      attggtgcg attttcacg gaagaccag aaagatagc 
      agcgtcgaa ttagcattc aaatatgcg gtctacaaa 
      attaacaaa gatagggtt ttgctttca aacaccact 
      ctagtatac gatatccaa tacgtgccc cgggacgac 
      agcttccga acgtcgaaa aaagtgtgt cgtcagatg 
      gaattcggc gtccaggcc atcttcggg ccctcggat 
      ccaatatta ggagcacat attcaaagt atttgcgaa 
      gctctagac gtgccccat ctcgaggct cgaatagac 
      ttcgagccc ctgtccaag gacttgtca atcaattta 
      cacccgagt caagagcac atgaacaag gcattcaaa 
      gacctgatg accttcctc aactggacc aaagtcgcc 
      attatatac gaggaagac tacggcctt ttcaagctg 
      caagaattg gtcaaggcg ccggctgct gcaaggact 
      gaaatgtat atccgccag gccggcccc acttcatat 
      cgacaagtg ctgaaagaa gtgagacaa aaagaaata 
      tacaagctc atcgtcgac acgaatccc agaaacatc 
      caaaaattt tttagagcg attttgcaa ctgcagatg 
      aacgactat cgctaccac tacatgttc accaccttt 
      gatttagag acattcgat ctggaagat ttcaaatac 
      aattctgta aatattacg gcattcaga attgttgat 
      gtggatgat ccccaagta aaagaatcc cttgaagtt 
      atggaaaaa ttccagccg attggccat gcaatcctt 
      aataaaagc gggattatt caggcggag cctgcgcta 
      atgtttgac tcagtgtat gtttttgct aaagggctg 
      gctgccatg ggttctata aagccaatg aacttatct 
      tgcgacgtc gagaaaccc tgggatgat ggatcgtcc 
      ctgtacaat tatttgggc gacgtggat gatctccga 
      ggtttgaca gggaacata gaatttaat ggtggcaag 
      aggtcaaac ttcaaactg gacctgctc aagctgaag 
      aaagaggag atccggaaa gtgggccag tggacgccg 
      tcggggggc gttaacatt actgacccc aacgctttc 
      tacgaaagt cacgcgccg aacataacc cttgtcgtc 
      atgactagg gaggagcgt ccgtacgtg atggtgaaa 
      gatgaaaaa aatttaacc ggaaacgcc agatacgag 
      ggcttttgc atcgaccta ttgaaatgg atcgcaggt 
      caggtcggc ttccagtac acaatccgt ctggtcccc 
      gatcacatg tatggagtg tacgacccc gataccaaa 
      gaatggaac ggcatcgtg cgcgagctt atggaaaaa 
      cgggcggac ctcgctgtg gcttccatg acaataaat 
      tatgcacgg gagagcgta atcgacttt actaaaccg 
      tttatgaac ctcggcata ggaattcta ttcaaggtt 
      cccaccagc caacccacg cgcctgttc agttttatg 
      aacccctta gcggtggaa atttggctt tacgtttta 
      gcggcgtat atgctggtg tcatttaca ttgttcgta 
      atggcgaga ttttcgccg tacgagtgg aacaacccc 
      cacccctgt caccaggaa tccgacatt gtcgagaac 
      caattctcg gtttccaat agtttttgg tttataacg 
      ggaacattt ttacgacaa ggatccggt ttaaatcct 
      aagcagtcg tcgaaaagc gagccgagt cggctgttt 
      tcctttctc aaccctttg gcgatgaac atatggctc 
      tacatggcc ggggcttac gtcctggta tcgataaca 
      atttggata gtggcccgg ttttcgccc cttgagtgg 
      aaagagccg gagctgtgc gacgactgt ctcctaaaa 
      aaatattca atcttgttc gagtttgac gaacattgc 
      gagcacgac gcatcatca ggcatcggc agcgacgcc 
      gctgacgat ttagcctcg cttcctaac aacgacaac 
      gatattgac gaagtaatc gaatccatt aatgatccg 
      tgtctaagt cacgaacac gccgacgga agaacatta 
      gaaatactc gagaacgga ttcacgata ggcaacagc 
      ttctggttt gccatcggc agtttgatg cagcaagga 
      tcagatctc aacccgaag gcaacgtct acgaggatc 
      gtaggcgga atctggtgg ttttttact ctcataatc 
      atctcttcc tacaccgca aatttggcc gccttccta 
      acggtggaa cgcatgatt acccccatt gagagtgcc 
      caagacctg gccgatcag accgatatt gcttatgga 
      actctcgaa ggcggcagc actatgacg ttttttagg 
      gattcgaag ataggcatt taccaaaaa atgtggcgg 
      tttatggag agccgaaag ccgtcggtg ttcgtgaaa 
      acgtacgag gaaggtgtg caacgcgtg ctggaggga 
      aattacgcc ttcctaatg gaatcaact atgctggac 
      tacgccgtt caaagagat tgcaattta acgcaaatt 
      ggaggcctg ctggactca aaaggctac gggatagcg 
      acgccgaaa ggcagtccg tggagagac aaaatatcg 
      cttgcaatc ctcgagctt caagagaaa ggagtgatc 
      caaatactc tacgacaag tggtggaaa aatactgga 
      gatgtttgt aatcgagac gataaaagt aaagaatcc 
      aaagccaac gccttgggg gtcgaaaat attgggggt 
      gtgtttgtg gtgcttctc tgcggcctg gccctcgcc 
      attctagtc gccatcctc gagttctgc tggaacagc 
      aagaaaaac gcccagacc gaccgccag tcgctgtgt 
      tcggaaatg gcagaagaa ttgcggttt gcggtgcgt 
      tgtcacggc tccagacag cgtcccgct ctgcgaaga 
      tcttgtaca aggtgtagt ccggccacg acatatgtg 
      cctgcggcc cttgattta ccgcacatc aatgggcca 
      atgaattta atggaaatc caatttatt cccgaatcg 
      agtcaacac gcgcaaagg cgtgatgct gcccatgat 
      ggacctgaa gaagtcgcc catcagcta cgacgtcga 
      cacttagcg gcgactcta cgctggggg cacgtcgac 
      gttcgcgtg cacctccag cgccatccg ggactgctg 
      cgggggccc gtccaactc ccgcccgca gcgggcggc 
      caccctcaa ttacagggc cactccgca gtgcccaga 
      tctggcgaa aaaaagtgc cacgctggg acgtaa
    mRNA sequence
    • >TC032415 RA
      aatcacacg ccgctccgg tagttcaga acgatcaag 
      cgcgtgctc atgatacga tcaccataa tcagaaaaa 
      atatttttc gagcagtgc gtaatctta ttgtgaaat 
      ctgaccaaa aggacacaa gtcgggtaa attggttct 
      gctgtcgtg ggtggcaat cattaaaag ttccgtctc 
      tggtttttc ttttattac cgttcgatt gctagcaat 
      tcttgtaag ttattttat ttgttgtaa caaacgtta 
      tttcaggtt tcaattcca tttcgttgc gaaatttaa 
      tttagataa gatctttat tgctatcta acatttcag 
      cttcgaaag gtccgttta aactatata aataaaatc 
      agtgtagtg taataatga attagataa actcgactc 
      aaagtgttc aaatatttg tggaccgga ccctcccta 
      aatcaatgt ttatctaag tgtattcta attataaac 
      attgttgat ggtattaat ttagtgtgg tgaatttgg 
      atgtgatgc tgcattgca tatgcagtg atatctggt 
      catgctgat tggtcgatc agtgatagc aaaatggag 
      gtgatatgt gtgctgata ctcgtttgg agtacgctg 
      acctcgaac gcgctgcca cctgtcatt cgcattggt 
      gcgattttc acggaagac cagaaagat agcagcgtc 
      gaattagca ttcaaatat gcggtctac aaaattaac 
      aaagatagg gttttgctt tcaaacacc actctagta 
      tacgatatc caatacgtg ccccgggac gacagcttc 
      cgaacgtcg aaaaaagtg tgtcgtcag atggaattc 
      ggcgtccag gccatcttc gggccctcg gatccaata 
      ttaggagca catattcaa agtatttgc gaagctcta 
      gacgtgccc catctcgag gctcgaata gacttcgag 
      cccctgtcc aaggacttg tcaatcaat ttacacccg 
      agtcaagag cacatgaac aaggcattc aaagacctg 
      atgaccttc ctcaactgg accaaagtc gccattata 
      tacgaggaa gactacggc cttttcaag ctgcaagaa 
      ttggtcaag gcgccggct gctgcaagg actgaaatg 
      tatatccgc caggccggc cccacttca tatcgacaa 
      gtgctgaaa gaagtgaga caaaaagaa atatacaag 
      ctcatcgtc gacacgaat cccagaaac atccaaaaa 
      ttttttaga gcgattttg caactgcag atgaacgac 
      tatcgctac cactacatg ttcaccacc tttgattta 
      gagacattc gatctggaa gatttcaaa tacaattct 
      gtaaatatt acggcattc agaattgtt gatgtggat 
      gatccccaa gtaaaagaa tcccttgaa gttatggaa 
      aaattccag ccgattggc catgcaatc cttaataaa 
      agcgggatt attcaggcg gagcctgcg ctaatgttt 
      gactcagtg tatgttttt gctaaaggg ctggctgcc 
      atgggttct ataaagcca atgaactta tcttgcgac 
      gtcgagaaa ccctgggat gatggatcg tccctgtac 
      aattatttg ggcgacgtg gatgatctc cgaggtttg 
      acagggaac atagaattt aatggtggc aagaggtca 
      aacttcaaa ctggacctg ctcaagctg aagaaagag 
      gagatccgg aaagtgggc cagtggacg ccgtcgggg 
      ggcgttaac attactgac cccaacgct ttctacgaa 
      agtcacgcg ccgaacata acccttgtc gtcatgact 
      agggaggag cgtccgtac gtgatggtg aaagatgaa 
      aaaaattta accggaaac gccagatac gagggcttt 
      tgcatcgac ctattgaaa tggatcgca ggtcaggtc 
      ggcttccag tacacaatc cgtctggtc cccgatcac 
      atgtatgga gtgtacgac cccgatacc aaagaatgg 
      aacggcatc gtgcgcgag cttatggaa aaacgggcg 
      gacctcgct gtggcttcc atgacaata aattatgca 
      cgggagagc gtaatcgac tttactaaa ccgtttatg 
      aacctcggc ataggaatt ctattcaag gttcccacc 
      agccaaccc acgcgcctg ttcagtttt atgaacccc 
      ttagcggtg gaaatttgg ctttacgtt ttagcggcg 
      tatatgctg gtgtcattt acattgttc gtaatggcg 
      agattttcg ccgtacgag tggaacaac ccccacccc 
      tgtcaccag gaatccgac attgtcgag aaccaattc 
      tcggtttcc aatagtttt tggtttata acgggaaca 
      tttttacga caaggatcc ggtttaaat cctaagcag 
      tcgtcgaaa agcgagccg agtcggctg ttttccttt 
      ctcaaccct ttggcgatg aacatatgg ctctacatg 
      gccggggct tacgtcctg gtatcgata acaatttgg 
      atagtggcc cggttttcg ccccttgag tggaaagag 
      ccggagctg tgcgacgac tgtctccta aaaaaatat 
      tcaatcttg ttcgagttt gacgaacat tgcgagcac 
      gacgcatca tcaggcatc ggcagcgac gccgctgac 
      gatttagcc tcgcttcct aacaacgac aacgatatt 
      gacgaagta atcgaatcc attaatgat ccgtgtcta 
      agtcacgaa cacgccgac ggaagaaca ttagaaata 
      ctcgagaac ggattcacg ataggcaac agcttctgg 
      tttgccatc ggcagtttg atgcagcaa ggatcagat 
      ctcaacccg aaggcaacg tctacgagg atcgtaggc 
      ggaatctgg tggtttttt actctcata atcatctct 
      tcctacacc gcaaatttg gccgccttc ctaacggtg 
      gaacgcatg attaccccc attgagagt gcccaagac 
      ctggccgat cagaccgat attgcttat ggaactctc 
      gaaggcggc agcactatg acgtttttt agggattcg 
      aagataggc atttaccaa aaaatgtgg cggtttatg 
      gagagccga aagccgtcg gtgttcgtg aaaacgtac 
      gaggaaggt gtgcaacgc gtgctggag ggaaattac 
      gccttccta atggaatca actatgctg gactacgcc 
      gttcaaaga gattgcaat ttaacgcaa attggaggc 
      ctgctggac tcaaaaggc tacgggata gcgacgccg 
      aaaggcagt ccgtggaga gacaaaata tcgcttgca 
      atcctcgag cttcaagag aaaggagtg atccaaata 
      ctctacgac aagtggtgg aaaaatact ggagatgtt 
      tgtaatcga gacgataaa agtaaagaa tccaaagcc 
      aacgccttg ggggtcgaa aatattggg ggtgtgttt 
      gtggtgctt ctctgcggc ctggccctc gccattcta 
      gtcgccatc ctcgagttc tgctggaac agcaagaaa 
      aacgcccag accgaccgc cagtcgctg tgttcggaa 
      atggcagaa gaattgcgg tttgcggtg cgttgtcac 
      ggctccaga cagcgtccc gctctgcga agatcttgt 
      acaaggtgt agtccggcc acgacatat gtgcctgcg 
      gcccttgat ttaccgcac atcaatggg gaaggcgtg 
      atgctgccc atgatggac ctgaagaag tcgcccatc 
      agctacgac gtcgacact tagcggcga ctctacgct 
      gggggcacg tcgacgttc gcgtgcacc tccagcgcc 
      atccgggac tgctgcggg ggcccgtcc aactcccgc 
      ccgcagcgg gcggccacc ctcaattac agggccact 
      ccgcagtgc ccagatctg gcgaaaaaa agtgccacg 
      ctgggacgt aactccgga ttctgtcgc gatacgtgt 
      gacattgta caaagcggt ctgcagaac gacgtcccg 
      gataaaata ctgtggaag ttcgattgt gatattggt 
      agtccgaac aacccagac gtagtgacc aggacactg 
      cccgacgcc atcgtaagt aggaccacc actttgtga 
      tacataggt agaatttgt aaattgaat caaataaat 
      ggactgatg tttgtacca atttcaata caattgtta 
      gggaattta cgctgattt caatgtttt acaaaaatc 
      gaattttag agactgact aaccagaat tcttaatca 
      aaaataaaa aacgcaaat ccgtctaaa tcttctacc 
      ctcgcccat attttccaa atcgaacct ttcgtcggc 
      ccaaattgc ctcagttcc ttccgaccc aagtcacgt 
      ctaaccaaa ttcgcggtg ttccaacaa agtaaatgg 
      tctataaag attttttag caacgcttt aatttttta 
      tgcctcatc tattcgtaa aactgaact gaaacgaca 
      catcgttta gagccagca atgaactca accataagc 
      tggaaactg gctaaatat tttatttaa ttgatttat 
      tttattccc tcgacggct ccgctttgt ccgactgag 
      actaaatga aagaaatga gaaaaaata aagcggatt 
      gaagaattg aaacttggg agttgttat tcaaacaat 
      cgtttatgt ttcgcaata aaatatcaa cttttaggg 
      gataaaagc ttcggaatt catactttg gctacaata 
      tttttacga tgctttaaa gttgtgagt tttacggcg 
      gcgttcaag taacacatc gtggaattt ttaccatta 
      attttgcac tgtgcaggg gaatatacg taaaattaa 
      aagcatatc ttttagggg aatgcggca gtctctaaa 
      attttggtt tgtttctca aaattggta atgacttca 
      acacagttc attaatttt aaataattt ccgtccatt 
      atatttcat tggtttgac ccgtaatta acgctaaat 
      cagtcggta ttaatgcaa caatcaaaa ttcttgtta 
      aagttttat tactaaaat aaccatcaa tgttatgaa 
      acataataa attggtttg tgttaa
    • >TC032415 RB
      aatcacacg ccgctccgg tagttcaga acgatcaag 
      cgcgtgctc atgatacga tcaccataa tcagaaaaa 
      atatttttc gagcagtgc gtaatctta ttgtgaaat 
      ctgaccaaa aggacacaa gtcgggtaa attggttct 
      gctgtcgtg ggtggcaat cattaaaag ttccgtctc 
      tggtttttc ttttattac cgttcgatt gctagcaat 
      tcttgtaag ttattttat ttgttgtaa caaacgtta 
      tttcaggtt tcaattcca tttcgttgc gaaatttaa 
      tttagataa gatctttat tgctatcta acatttcag 
      cttcgaaag gtccgttta aactatata aataaaatc 
      agtgtagtg taataatga attagataa actcgactc 
      aaagtgttc aaatatttg tggaccgga ccctcccta 
      aatcaatgt ttatctaag tgtattcta attataaac 
      attgttgat ggtattaat ttagtgtgg tgaatttgg 
      atgtgatgc tgcattgca tatgcagtg atatctggt 
      catgctgat tggtcgatc agtgatagc aaaatggag 
      gtgatatgt gtgctgata ctcgtttgg agtacgctg 
      acctcgaac gcgctgcca cctgtcatt cgcattggt 
      gcgattttc acggaagac cagaaagat agcagcgtc 
      gaattagca ttcaaatat gcggtctac aaaattaac 
      aaagatagg gttttgctt tcaaacacc actctagta 
      tacgatatc caatacgtg ccccgggac gacagcttc 
      cgaacgtcg aaaaaagtg tgtcgtcag atggaattc 
      ggcgtccag gccatcttc gggccctcg gatccaata 
      ttaggagca catattcaa agtatttgc gaagctcta 
      gacgtgccc catctcgag gctcgaata gacttcgag 
      cccctgtcc aaggacttg tcaatcaat ttacacccg 
      agtcaagag cacatgaac aaggcattc aaagacctg 
      atgaccttc ctcaactgg accaaagtc gccattata 
      tacgaggaa gactacggc cttttcaag ctgcaagaa 
      ttggtcaag gcgccggct gctgcaagg actgaaatg 
      tatatccgc caggccggc cccacttca tatcgacaa 
      gtgctgaaa gaagtgaga caaaaagaa atatacaag 
      ctcatcgtc gacacgaat cccagaaac atccaaaaa 
      ttttttaga gcgattttg caactgcag atgaacgac 
      tatcgctac cactacatg ttcaccacc tttgattta 
      gagacattc gatctggaa gatttcaaa tacaattct 
      gtaaatatt acggcattc agaattgtt gatgtggat 
      gatccccaa gtaaaagaa tcccttgaa gttatggaa 
      aaattccag ccgattggc catgcaatc cttaataaa 
      agcgggatt attcaggcg gagcctgcg ctaatgttt 
      gactcagtg tatgttttt gctaaaggg ctggctgcc 
      atgggttct ataaagcca atgaactta tcttgcgac 
      gtcgagaaa ccctgggat gatggatcg tccctgtac 
      aattatttg ggcgacgtg gatgatctc cgaggtttg 
      acagggaac atagaattt aatggtggc aagaggtca 
      aacttcaaa ctggacctg ctcaagctg aagaaagag 
      gagatccgg aaagtgggc cagtggacg ccgtcgggg 
      ggcgttaac attactgac cccaacgct ttctacgaa 
      agtcacgcg ccgaacata acccttgtc gtcatgact 
      agggaggag cgtccgtac gtgatggtg aaagatgaa 
      aaaaattta accggaaac gccagatac gagggcttt 
      tgcatcgac ctattgaaa tggatcgca ggtcaggtc 
      ggcttccag tacacaatc cgtctggtc cccgatcac 
      atgtatgga gtgtacgac cccgatacc aaagaatgg 
      aacggcatc gtgcgcgag cttatggaa aaacgggcg 
      gacctcgct gtggcttcc atgacaata aattatgca 
      cgggagagc gtaatcgac tttactaaa ccgtttatg 
      aacctcggc ataggaatt ctattcaag gttcccacc 
      agccaaccc acgcgcctg ttcagtttt atgaacccc 
      ttagcggtg gaaatttgg ctttacgtt ttagcggcg 
      tatatgctg gtgtcattt acattgttc gtaatggcg 
      agattttcg ccgtacgag tggaacaac ccccacccc 
      tgtcaccag gaatccgac attgtcgag aaccaattc 
      tcggtttcc aatagtttt tggtttata acgggaaca 
      tttttacga caaggatcc ggtttaaat cctaagcag 
      tcgtcgaaa agcgagccg agtcggctg ttttccttt 
      ctcaaccct ttggcgatg aacatatgg ctctacatg 
      gccggggct tacgtcctg gtatcgata acaatttgg 
      atagtggcc cggttttcg ccccttgag tggaaagag 
      ccggagctg tgcgacgac tgtctccta aaaaaatat 
      tcaatcttg ttcgagttt gacgaacat tgcgagcac 
      gacgcatca tcaggcatc ggcagcgac gccgctgac 
      gatttagcc tcgcttcct aacaacgac aacgatatt 
      gacgaagta atcgaatcc attaatgat ccgtgtcta 
      agtcacgaa cacgccgac ggaagaaca ttagaaata 
      ctcgagaac ggattcacg ataggcaac agcttctgg 
      tttgccatc ggcagtttg atgcagcaa ggatcagat 
      ctcaacccg aaggcaacg tctacgagg atcgtaggc 
      ggaatctgg tggtttttt actctcata atcatctct 
      tcctacacc gcaaatttg gccgccttc ctaacggtg 
      gaacgcatg attaccccc attgagagt gcccaagac 
      ctggccgat cagaccgat attgcttat ggaactctc 
      gaaggcggc agcactatg acgtttttt agggattcg 
      aagataggc atttaccaa aaaatgtgg cggtttatg 
      gagagccga aagccgtcg gtgttcgtg aaaacgtac 
      gaggaaggt gtgcaacgc gtgctggag ggaaattac 
      gccttccta atggaatca actatgctg gactacgcc 
      gttcaaaga gattgcaat ttaacgcaa attggaggc 
      ctgctggac tcaaaaggc tacgggata gcgacgccg 
      aaaggcagt ccgtggaga gacaaaata tcgcttgca 
      atcctcgag cttcaagag aaaggagtg atccaaata 
      ctctacgac aagtggtgg aaaaatact ggagatgtt 
      tgtaatcga gacgataaa agtaaagaa tccaaagcc 
      aacgccttg ggggtcgaa aatattggg ggtgtgttt 
      gtggtgctt ctctgcggc ctggccctc gccattcta 
      gtcgccatc ctcgagttc tgctggaac agcaagaaa 
      aacgcccag accgaccgc cagtcgctg tgttcggaa 
      atggcagaa gaattgcgg tttgcggtg cgttgtcac 
      ggctccaga cagcgtccc gctctgcga agatcttgt 
      acaaggtgt agtccggcc acgacatat gtgcctgcg 
      gcccttgat ttaccgcac atcaatggg ccaatgaat 
      ttaatggaa atccaattt attcccgaa tcgagtcaa 
      cacgcgcaa aggcgtgat gctgcccat gatggacct 
      gaagaagtc gcccatcag ctacgacgt cgacactta 
      gcggcgact ctacgctgg gggcacgtc gacgttcgc 
      gtgcacctc cagcgccat ccgggactg ctgcggggg 
      cccgtccaa ctcccgccc gcagcgggc ggccaccct 
      caattacag ggccactcc gcagtgccc agatctggc 
      gaaaaaaag tgccacgct gggacgtaa ctccggatt 
      ctgtcgcga tacgtgtga cattgtaca aagcggtct 
      gcagaacga cgtcccgga taaaatact gtggaagtt 
      cgattgtga tattggtag tccgaacaa cccagacgt 
      agtgaccag gacactgcc cgacgccat cgtaagtag 
      gaccaccac tttgtgata cataggtag aatttgtaa 
      attgaatca aataaatgg actgatgtt tgtaccaat 
      ttcaataca attgttagg gaatttacg ctgatttca 
      atgttttac aaaaatcga attttagag actgactaa 
      ccagaattc ttaatcaaa aataaaaaa cgcaaatcc 
      gtctaaatc ttctaccct cgcccatat tttccaaat 
      cgaaccttt cgtcggccc aaattgcct cagttcctt 
      ccgacccaa gtcacgtct aaccaaatt cgcggtgtt 
      ccaacaaag taaatggtc tataaagat tttttagca 
      acgctttaa ttttttatg cctcatcta ttcgtaaaa 
      ctgaactga aacgacaca tcgtttaga gccagcaat 
      gaactcaac cataagctg gaaactggc taaatattt 
      tatttaatt gatttattt tattccctc gacggctcc 
      gctttgtcc gactgagac taaatgaaa gaaatgaga 
      aaaaataaa gcggattga agaattgaa acttgggag 
      ttgttattc aaacaatcg tttatgttt cgcaataaa 
      atatcaact tttagggga taaaagctt cggaattca 
      tactttggc tacaatatt tttacgatg ctttaaagt 
      tgtgagttt tacggcggc gttcaagta acacatcgt 
      ggaattttt accattaat tttgcactg tgcagggga 
      atatacgta aaattaaaa gcatatctt ttaggggaa 
      tgcggcagt ctctaaaat tttggtttg tttctcaaa 
      attggtaat gacttcaac acagttcat taattttaa 
      ataatttcc gtccattat atttcattg gtttgaccc 
      gtaattaac gctaaatca gtcggtatt aatgcaaca 
      atcaaaatt cttgttaaa gttttatta ctaaaataa 
      ccatcaatg ttatgaaac ataataaat tggtttgtg 
      ttaa
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