Status
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Gene information
General information
TC002866 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 7835816..7838858
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002866
    iBeetle screen iB_03459

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002866 RA
      MLLKRSIFL QVCLLLTLK QTKCQDYES GYGVDESDD 
      HSEAQEDSG PTDYSKYYA VDRSAPLIS SAQDFGASV 
      KQEVQQPKN PFAAQTKSL GFISEQSQF PNFYAAGHF 
      PVDYFEEAQ KTQRQENQE DDGFEPSGG VDYISHNLP 
      LNLDGEYTS EVEKVQPKP NKPKRKSKK VRKYDAEKE 
      ASEEFKNPD YSYEYEPSS SGKYTDYNS GKSSSTVSQ 
      QPTVAGFYE KLTEAPTAT PYQKYSFQP TSPENKFLG 
      SLISTVAPF NPSYSKIQS TAVTPTFET QPSENIPSH 
      LKDKNCRRV RKHIGDGRR RKREAMNCY VCEDQANNS 
      KYTQCSYAV EPEPTNDYS GISERYSTP AKQPDGFRY 
      KRYSGDDNV DPYEYIKSR TQKAFTDAQ ETKDYEEYK 
      IPDFYVDEN PDKSYSEIQ SEAIAKNPA NCHKEESNG 
      MTCTICKDP KTGGNFEQC SYTSEPKEQ KYAYVTEKK 
      YDSDEPEEG AESKTQPQK EEADKESKE EEEGEKKFD 
      SSEESEKVK FDDKLDFKS SNDKPKFSS KIKEYKPLV 
      LPSNDNSQF AKLDEFKPI GLSSNEDSK YSKLEDFKP 
      SEEYKASYR GHDKFKLSD DYNPEIKDD PTSKKEPYN 
      PPEGYQKGE QGGSGGPKK LIDDDPYDI PKHFAESTI 
      KEQKTGVRG LEPSLYGSA DSSEVEGDD ADSSSDVEP 
      YKDIDEYHF KLFPEFSNE ESQQAEVVA ANPSADATR 
      KDVEEVLAE FSKKDRSAC KKAEKNGMT CYLCVDKKG 
      IQHEECMYV SESKPQASH VAYHEVKQI KSPNGETKE 
      LEQVPAATE IKRKHFFKK VVTTPTPFS MEAAASNEY 
      ETKIKYNPS KKRKSAKKV KEPVQAASD DDPEKLPED 
      DPEKLPEDE NEPETPAEF VVGDEEGAY SAETKPVYS 
      KVLGVKLPK YMIEKSEFE KEFDEFAGT H
    CDS sequence
    • >TC002866 RA
      atgcttcta aaaagaagc atttttcta caggtctgt 
      cttttgctc acactcaaa caaaccaaa tgccaggac 
      tacgaaagc ggctacggt gtcgacgaa agtgacgac 
      cactccgag gcccaggaa gattccggg ccgacagac 
      tactcaaaa tattacgct gttgacagg tcagcgcct 
      ctgatctcc tcggctcaa gattttgga gcctcggtc 
      aagcaggag gtccaacaa ccgaaaaac ccgttcgcg 
      gcccaaacc aagagtttg ggattcatt tcggaacaa 
      agccagttc ccgaatttc tacgccgcc ggacatttc 
      ccagttgac tatttcgaa gaagcccaa aaaacgcaa 
      agacaagag aaccaagag gacgacggt tttgaacca 
      tcgggtggc gttgactac atttcccac aacctgcca 
      ctgaacctg gacggtgaa tacacctca gaagtggag 
      aaagtccaa ccgaagccc aacaagccc aagaggaag 
      agcaagaag gtgcgcaag tacgatgcc gaaaaagaa 
      gcatctgaa gaattcaaa aacccggac tattcctat 
      gaatacgaa ccgtcatcc tccgggaaa tacactgat 
      tacaattcc gggaagtcc agttcgact gtgtcccaa 
      caacccaca gttgcgggt ttttacgag aaacttacc 
      gaagcccca acggccacc ccgtaccag aaatacagt 
      tttcaacca actagccct gagaacaaa tttttgggg 
      tccttgatc agcactgtt gcccctttt aacccctcc 
      tactccaaa atccaaagc actgcggtt accccgact 
      tttgagacc cagccaagt gaaaacatc ccaagtcac 
      ttgaaagat aagaactgt agaagggtc aggaaacac 
      atcggcgat ggaagaaga agaaagagg gaagctatg 
      aactgttac gtctgtgaa gaccaggca aataactca 
      aaatacacc caatgttcg tatgctgta gaaccggaa 
      ccaaccaat gattacagc gggatttcg gaaagatac 
      tccactcca gccaagcaa cctgacggt ttccgctac 
      aagagatat tccggtgat gacaatgtt gacccctat 
      gagtacatc aagtcccgg acgcagaaa gccttcacc 
      gacgctcaa gaaaccaaa gattacgaa gaatacaag 
      atcccggac ttttacgta gatgaaaat ccggataag 
      agctactcg gaaatacag tctgaagca atcgccaaa 
      aacccagcc aattgtcac aaggaggaa agcaatgga 
      atgacttgc accatctgc aaagacccc aaaactggg 
      ggcaatttc gaacagtgt tcctacact tctgagccc 
      aaagaacag aaatatgcg tacgtgacg gaaaagaag 
      tacgacagt gacgagcct gaggaaggg gccgaatcg 
      aaaacgcaa ccccagaag gaggaagca gataaggaa 
      agtaaagaa gaagaagaa ggggaaaag aaatttgac 
      tcgtctgaa gaaagtgaa aaagtaaaa tttgacgat 
      aagttggat ttcaaatcg agtaatgac aaaccaaag 
      ttcagtagc aaaattaaa gagtataaa ccgttagtt 
      ttgccaagt aatgataat tcccagttt gccaagttg 
      gacgagttt aaaccaatc ggtttgtcg agtaacgag 
      gattcaaaa tatagcaaa ctggaggat ttcaaacct 
      tctgaagag tacaaagcg agttatcgc ggacacgac 
      aaatttaaa ctgtcggat gattacaat cctgaaata 
      aaggacgac cctacgtca aagaaggag ccctacaac 
      ccccctgaa ggttaccaa aagggggaa caagggggt 
      tcaggaggt cctaaaaaa ctgatcgat gatgatccg 
      tacgatatt ccaaaacac ttcgctgaa agcacaatc 
      aaggagcaa aaaactggc gttcggggt ttggaacca 
      agtctctac ggaagtgcc gactcctca gaagtcgaa 
      ggcgacgac gccgattct tccagcgat gttgagcct 
      tacaaagac attgacgag taccatttc aaactcttc 
      cccgagttc tccaacgag gagtcgcaa caagccgag 
      gtcgtggca gcgaatccg tccgccgac gccacccgg 
      aaagacgtg gaggaggtg ttggccgag ttcagcaaa 
      aaggaccgt tctgcgtgc aagaaagcc gagaagaac 
      ggcatgacc tgctacctg tgcgtggac aagaagggg 
      atccagcac gaggagtgc atgtacgtg tcggagtcc 
      aagccccag gcgagccat gtggcgtac cacgaagtc 
      aaacagatc aaatccccg aatggtgaa accaaggag 
      ctcgagcag gtcccagcc gccacggag atcaagcgc 
      aaacatttc tttaagaaa gttgtgacc acgcctacg 
      cctttctcc atggaagcg gcagcgtcg aatgagtac 
      gagaccaaa ataaagtac aacccgagc aaaaagagg 
      aaaagtgcg aagaaagtg aaagagccc gttcaagcg 
      gcgagcgac gacgatccc gagaagttg ccggaagac 
      gaccccgag aagctgccc gaggacgag aacgagccc 
      gagacgccc gcagagttc gtcgtgggg gatgaggaa 
      ggggcgtac agtgccgag acgaagcca gtctacagc 
      aaggtgctg ggggtgaag ctccccaag tacatgatt 
      gagaaatca gagtttgag aaggagttt gatgagttc 
      gctggtaca cattag
    mRNA sequence
    • >TC002866 RA
      ttcgtgctt gctcatcaa gtgacgttc aaaatgctt 
      ctaaaaaga agcattttt ctacaggtc tgtcttttg 
      ctcacactc aaacaaacc aaatgccag gactacgaa 
      agcggctac ggtgtcgac gaaagtgac gaccactcc 
      gaggcccag gaagattcc gggccgaca gactactca 
      aaatattac gctgttgac aggtcagcg cctctgatc 
      tcctcggct caagatttt ggagcctcg gtcaagcag 
      gaggtccaa caaccgaaa aacccgttc gcggcccaa 
      accaagagt ttgggattc atttcggaa caaagccag 
      ttcccgaat ttctacgcc gccggacat ttcccagtt 
      gactatttc gaagaagcc caaaaaacg caaagacaa 
      gagaaccaa gaggacgac ggttttgaa ccatcgggt 
      ggcgttgac tacatttcc cacaacctg ccactgaac 
      ctggacggt gaatacacc tcagaagtg gagaaagtc 
      caaccgaag cccaacaag cccaagagg aagagcaag 
      aaggtgcgc aagtacgat gccgaaaaa gaagcatct 
      gaagaattc aaaaacccg gactattcc tatgaatac 
      gaaccgtca tcctccggg aaatacact gattacaat 
      tccgggaag tccagttcg actgtgtcc caacaaccc 
      acagttgcg ggtttttac gagaaactt accgaagcc 
      ccaacggcc accccgtac cagaaatac agttttcaa 
      ccaactagc cctgagaac aaatttttg gggtccttg 
      atcagcact gttgcccct tttaacccc tcctactcc 
      aaaatccaa agcactgcg gttaccccg acttttgag 
      acccagcca agtgaaaac atcccaagt cacttgaaa 
      gataagaac tgtagaagg gtcaggaaa cacatcggc 
      gatggaaga agaagaaag agggaagct atgaactgt 
      tacgtctgt gaagaccag gcaaataac tcaaaatac 
      acccaatgt tcgtatgct gtagaaccg gaaccaacc 
      aatgattac agcgggatt tcggaaaga tactccact 
      ccagccaag caacctgac ggtttccgc tacaagaga 
      tattccggt gatgacaat gttgacccc tatgagtac 
      atcaagtcc cggacgcag aaagccttc accgacgct 
      caagaaacc aaagattac gaagaatac aagatcccg 
      gacttttac gtagatgaa aatccggat aagagctac 
      tcggaaata cagtctgaa gcaatcgcc aaaaaccca 
      gccaattgt cacaaggag gaaagcaat ggaatgact 
      tgcaccatc tgcaaagac cccaaaact gggggcaat 
      ttcgaacag tgttcctac acttctgag cccaaagaa 
      cagaaatat gcgtacgtg acggaaaag aagtacgac 
      agtgacgag cctgaggaa ggggccgaa tcgaaaacg 
      caaccccag aaggaggaa gcagataag gaaagtaaa 
      gaagaagaa gaaggggaa aagaaattt gactcgtct 
      gaagaaagt gaaaaagta aaatttgac gataagttg 
      gatttcaaa tcgagtaat gacaaacca aagttcagt 
      agcaaaatt aaagagtat aaaccgtta gttttgcca 
      agtaatgat aattcccag tttgccaag ttggacgag 
      tttaaacca atcggtttg tcgagtaac gaggattca 
      aaatatagc aaactggag gatttcaaa ccttctgaa 
      gagtacaaa gcgagttat cgcggacac gacaaattt 
      aaactgtcg gatgattac aatcctgaa ataaaggac 
      gaccctacg tcaaagaag gagccctac aacccccct 
      gaaggttac caaaagggg gaacaaggg ggttcagga 
      ggtcctaaa aaactgatc gatgatgat ccgtacgat 
      attccaaaa cacttcgct gaaagcaca atcaaggag 
      caaaaaact ggcgttcgg ggtttggaa ccaagtctc 
      tacggaagt gccgactcc tcagaagtc gaaggcgac 
      gacgccgat tcttccagc gatgttgag ccttacaaa 
      gacattgac gagtaccat ttcaaactc ttccccgag 
      ttctccaac gaggagtcg caacaagcc gaggtcgtg 
      gcagcgaat ccgtccgcc gacgccacc cggaaagac 
      gtggaggag gtgttggcc gagttcagc aaaaaggac 
      cgttctgcg tgcaagaaa gccgagaag aacggcatg 
      acctgctac ctgtgcgtg gacaagaag gggatccag 
      cacgaggag tgcatgtac gtgtcggag tccaagccc 
      caggcgagc catgtggcg taccacgaa gtcaaacag 
      atcaaatcc ccgaatggt gaaaccaag gagctcgag 
      caggtccca gccgccacg gagatcaag cgcaaacat 
      ttctttaag aaagttgtg accacgcct acgcctttc 
      tccatggaa gcggcagcg tcgaatgag tacgagacc 
      aaaataaag tacaacccg agcaaaaag aggaaaagt 
      gcgaagaaa gtgaaagag cccgttcaa gcggcgagc 
      gacgacgat cccgagaag ttgccggaa gacgacccc 
      gagaagctg cccgaggac gagaacgag cccgagacg 
      cccgcagag ttcgtcgtg ggggatgag gaaggggcg 
      tacagtgcc gagacgaag ccagtctac agcaaggtg 
      ctgggggtg aagctcccc aagtacatg attgagaaa 
      tcagagttt gagaaggag tttgatgag ttcgctggt 
      acacattag gagtaggct ttatgtctt gtgatagat 
      taattactt aggtacttg tgtaatata gttatgtta 
      ttaaa
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