General information | |||
TC002825 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 8828125..8834048 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC002825 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03457 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
- >TC002825 RA
MKLNVDGLI VYFPYDYIY PEQYAYMCE LKRALDAKG HCLLEMPSG TGKTTTILS LVVAYMLEH PHDVRKLIY CSRTVPEIE KVMEELKKL LEYYEKLDG EYPHLLGLV LSSRKNLCI HPEVSTERE GKIVDGKCH ALTASYIRE RHNYDDTTP ICQYYEGFA LDGKESTMP FGVYSIDDL KQYGRDRNW CPYFLSRFV ISYANIVVY SYHYLLDPK IAEVVSKEL TRESVVIFD EAHNIDNVC IDSMSVKIN KRIIDRASA NIQLLERTV AEMREDDHK KLQEEYQRL VEGLRDASV ARDTDVILA NPVLPDAIL QEAVPGNIR NAEHFVSFL KRFLEYLKT RLRVQHVVQ ESPAGFLKD VQSKVCIER KPLRFCAER LASLLRTLE ISDLTDFSP VILVTHLAT LVATYTKGF TIIVEPFDD KTPTVSNPI LHFSCLDSS IAIKPVFDR FQTVVITSG TLSPLDMYP KILNFHPVI MSSFTMTLA RPCLLPMIV SKGNDQVAI SSKFETRED NAVIRNYGQ LLVEIAANV PDGIVCFFT SYLYLESVV ASWYDQGVI DSLQRYKLL FIETQDSAE TSFALMYYI KACESGRGA VLLSVARGK VSEGVDFDH HLGRAVLMF GIPYVYTQS RILKARLDY LRDQFQIRE NDFLTFDAM RHAAQCVGR AIRGKTDYG IMIFADKRF SRSDKRSKL PKWIQEHLK DSLTNLSTE EAVQIAKRW LRQMAQPFT REDQLGVSL LTLEQLQNL EASKIQEQA QQN
- >TC002825 RA
atgaaacta aacgtcgat ggtttaatc gtgtacttc ccctacgac tacatctac cccgagcag tatgcctac atgtgcgag ctcaagcgg gccctggac gccaaaggc cactgcctt ttggaaatg ccctcaggg acgggtaag accaccact atcctctcc ctcgttgtt gcgtacatg ctcgagcac ccccatgac gtccgcaaa ttgatctac tgctctcgt actgtcccc gaaatcgag aaagttatg gaagaattg aaaaaactg ctcgagtat tatgagaag ttggacggg gagtacccc catctcctg gggctggtt ttgtcctca cggaagaat ctgtgtatt cacccggaa gtgagtact gagcgcgag ggtaagatt gtggacgga aaatgccac gcactaaca gcgagttac ataagggag aggcacaat tatgatgat acaactccc atttgtcag tattacgaa ggtttcgct ttggatggc aaagaaagt acgatgccg tttggggtt tacagtata gatgatttg aaacagtac ggacgggac cgcaactgg tgcccctat ttcctgtct cgttttgtg ataagttac gcgaatatc gtagtctac agttaccac tatttactg gaccccaaa atcgccgaa gtcgtctcc aaagagctg acacgagag tcggttgtt attttcgat gaggctcac aatatcgat aatgtttgc atcgactct atgagtgtg aaaatcaac aaacgaatt atcgatcga gcttccgcc aatattcag ttattggaa cgtactgtg gccgagatg cgtgaagac gatcacaaa aaattgcaa gaagaatat cagagatta gttgagggg ttgcgcgac gctagcgtg gcgcgagac actgacgtc attttggcc aatccggtc ttgcccgac gccatcctc caggaggcc gtccctgga aatattcgc aacgccgag cactttgtc agtttcctg aaacgtttc ctcgaatac ctcaaaact cggctgcga gtccagcac gtggtgcaa gaaagccct gctggattt ttaaaagac gtccaatcc aaagtctgt attgaacga aaaccgtta cgtttctgt gccgaacgc ttggcatct ctgttgaga actttggaa atatcagac ttgaccgac ttcagtccg gtcatttta gtcacacat ttggccact ttggtggcc acttacacg aaaggtttt acgataatc gtagaaccg tttgatgat aagacaccg acggtgtca aacccgatc ttacatttc agttgtttg gattcgtcg attgccata aaaccggtt tttgatcgg tttcaaacc gtggttata acctctgga accctatcc cctctagat atgtacccc aagattttg aacttccat cctgtgatc atgtcatcg ttcactatg acgttggcc aggccttgt ttgctacca atgatcgtg tcaaaaggc aacgaccaa gtcgcaatt tcgtcaaaa ttcgagaca cgcgaagac aatgctgtt atccgaaac tacggccaa cttctggtc gaaatagcc gccaatgtc cccgacgga atcgtctgt ttcttcacc tcttatctc tacctggaa tcggtcgtg gccagttgg tacgaccaa ggcgtcatc gacagcctc caaagatac aaactcctt ttcatcgag acccaggac tcggcagaa acgagcttc gccctcatg tactacatc aaagcttgc gagtcgggc cgcggggcc gtgctgctc tccgtcgcg cggggcaaa gtctccgaa ggggtcgac ttcgaccac cacctgggg cgcgcagtc ctcatgttc ggcattccc tacgtgtac acccagagc cgcatcctc aaagcgcgc ctcgactac cttcgggac cagttccag atccgggag aacgatttt ttgaccttt gacgccatg agacacgcg gcccagtgc gtggggagg gctatcagg gggaagacc gactacggt atcatgatt ttcgccgat aagaggttt tccaggtcg gataagcgg tcaaagttg cccaagtgg atccaggag catttgaag gactctctg acgaatctg tcgacggag gaggcggtc cagattgcc aagcggtgg ttgcgacag atggcgcag ccgttcact agggaggac cagttgggg gtgtctttg ctcactttg gagcagttg cagaatttg gaggcgagt aaaatccaa gagcaggcg caacaaaat tga
- >TC002825 RA
ggttccagc tcacatgac ttaaatttt taggccccc ttcttcctt actaatcga ttagcaaca ataaaaacc tttctattg ttagaaaat attacaaaa aattgcata accgcactt ttttccaaa catgccata acctaaaaa aactaccgg gtcaatgta aacaccacg gtttcccca agcacctca caaaaaatg aaactaaac gtcgatggt ttaatcgtg tacttcccc tacgactac atctacccc gagcagtat gcctacatg tgcgagctc aagcgggcc ctggacgcc aaaggccac tgccttttg gaaatgccc tcagggacg ggtaagacc accactatc ctctccctc gttgttgcg tacatgctc gagcacccc catgacgtc cgcaaattg atctactgc tctcgtact gtccccgaa atcgagaaa gttatggaa gaattgaaa aaactgctc gagtattat gagaagttg gacggggag tacccccat ctcctgggg ctggttttg tcctcacgg aagaatctg tgtattcac ccggaagtg agtactgag cgcgagggt aagattgtg gacggaaaa tgccacgca ctaacagcg agttacata agggagagg cacaattat gatgataca actcccatt tgtcagtat tacgaaggt ttcgctttg gatggcaaa gaaagtacg atgccgttt ggggtttac agtatagat gatttgaaa cagtacgga cgggaccgc aactggtgc ccctatttc ctgtctcgt tttgtgata agttacgcg aatatcgta gtctacagt taccactat ttactggac cccaaaatc gccgaagtc gtctccaaa gagctgaca cgagagtcg gttgttatt ttcgatgag gctcacaat atcgataat gtttgcatc gactctatg agtgtgaaa atcaacaaa cgaattatc gatcgagct tccgccaat attcagtta ttggaacgt actgtggcc gagatgcgt gaagacgat cacaaaaaa ttgcaagaa gaatatcag agattagtt gaggggttg cgcgacgct agcgtggcg cgagacact gacgtcatt ttggccaat ccggtcttg cccgacgcc atcctccag gaggccgtc cctggaaat attcgcaac gccgagcac tttgtcagt ttcctgaaa cgtttcctc gaatacctc aaaactcgg ctgcgagtc cagcacgtg gtgcaagaa agccctgct ggattttta aaagacgtc caatccaaa gtctgtatt gaacgaaaa ccgttacgt ttctgtgcc gaacgcttg gcatctctg ttgagaact ttggaaata tcagacttg accgacttc agtccggtc attttagtc acacatttg gccactttg gtggccact tacacgaaa ggttttacg ataatcgta gaaccgttt gatgataag acaccgacg gtgtcaaac ccgatctta catttcagt tgtttggat tcgtcgatt gccataaaa ccggttttt gatcggttt caaaccgtg gttataacc tctggaacc ctatcccct ctagatatg taccccaag attttgaac ttccatcct gtgatcatg tcatcgttc actatgacg ttggccagg ccttgtttg ctaccaatg atcgtgtca aaaggcaac gaccaagtc gcaatttcg tcaaaattc gagacacgc gaagacaat gctgttatc cgaaactac ggccaactt ctggtcgaa atagccgcc aatgtcccc gacggaatc gtctgtttc ttcacctct tatctctac ctggaatcg gtcgtggcc agttggtac gaccaaggc gtcatcgac agcctccaa agatacaaa ctccttttc atcgagacc caggactcg gcagaaacg agcttcgcc ctcatgtac tacatcaaa gcttgcgag tcgggccgc ggggccgtg ctgctctcc gtcgcgcgg ggcaaagtc tccgaaggg gtcgacttc gaccaccac ctggggcgc gcagtcctc atgttcggc attccctac gtgtacacc cagagccgc atcctcaaa gcgcgcctc gactacctt cgggaccag ttccagatc cgggagaac gattttttg acctttgac gccatgaga cacgcggcc cagtgcgtg gggagggct atcaggggg aagaccgac tacggtatc atgattttc gccgataag aggttttcc aggtcggat aagcggtca aagttgccc aagtggatc caggagcat ttgaaggac tctctgacg aatctgtcg acggaggag gcggtccag attgccaag cggtggttg cgacagatg gcgcagccg ttcactagg gaggaccag ttgggggtg tctttgctc actttggag cagttgcag aatttggag gcgagtaaa atccaagag caggcgcaa caaaattga tttgttatt attttttac gtaataaag tattttcaa gaaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
