Status
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Gene information
General information
TC002825 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 8828125..8834048
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002825
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03457

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002825 RA
      MKLNVDGLI VYFPYDYIY PEQYAYMCE LKRALDAKG 
HCLLEMPSG TGKTTTILS LVVAYMLEH PHDVRKLIY 
CSRTVPEIE KVMEELKKL LEYYEKLDG EYPHLLGLV 
LSSRKNLCI HPEVSTERE GKIVDGKCH ALTASYIRE 
RHNYDDTTP ICQYYEGFA LDGKESTMP FGVYSIDDL 
KQYGRDRNW CPYFLSRFV ISYANIVVY SYHYLLDPK 
IAEVVSKEL TRESVVIFD EAHNIDNVC IDSMSVKIN 
KRIIDRASA NIQLLERTV AEMREDDHK KLQEEYQRL 
VEGLRDASV ARDTDVILA NPVLPDAIL QEAVPGNIR 
NAEHFVSFL KRFLEYLKT RLRVQHVVQ ESPAGFLKD 
VQSKVCIER KPLRFCAER LASLLRTLE ISDLTDFSP 
VILVTHLAT LVATYTKGF TIIVEPFDD KTPTVSNPI 
LHFSCLDSS IAIKPVFDR FQTVVITSG TLSPLDMYP 
KILNFHPVI MSSFTMTLA RPCLLPMIV SKGNDQVAI 
SSKFETRED NAVIRNYGQ LLVEIAANV PDGIVCFFT 
SYLYLESVV ASWYDQGVI DSLQRYKLL FIETQDSAE 
TSFALMYYI KACESGRGA VLLSVARGK VSEGVDFDH 
HLGRAVLMF GIPYVYTQS RILKARLDY LRDQFQIRE 
NDFLTFDAM RHAAQCVGR AIRGKTDYG IMIFADKRF 
SRSDKRSKL PKWIQEHLK DSLTNLSTE EAVQIAKRW 
LRQMAQPFT REDQLGVSL LTLEQLQNL EASKIQEQA 
QQN
    CDS sequence
    • >TC002825 RA
      atgaaacta aacgtcgat ggtttaatc gtgtacttc 
ccctacgac tacatctac cccgagcag tatgcctac 
atgtgcgag ctcaagcgg gccctggac gccaaaggc 
cactgcctt ttggaaatg ccctcaggg acgggtaag 
accaccact atcctctcc ctcgttgtt gcgtacatg 
ctcgagcac ccccatgac gtccgcaaa ttgatctac 
tgctctcgt actgtcccc gaaatcgag aaagttatg 
gaagaattg aaaaaactg ctcgagtat tatgagaag 
ttggacggg gagtacccc catctcctg gggctggtt 
ttgtcctca cggaagaat ctgtgtatt cacccggaa 
gtgagtact gagcgcgag ggtaagatt gtggacgga 
aaatgccac gcactaaca gcgagttac ataagggag 
aggcacaat tatgatgat acaactccc atttgtcag 
tattacgaa ggtttcgct ttggatggc aaagaaagt 
acgatgccg tttggggtt tacagtata gatgatttg 
aaacagtac ggacgggac cgcaactgg tgcccctat 
ttcctgtct cgttttgtg ataagttac gcgaatatc 
gtagtctac agttaccac tatttactg gaccccaaa 
atcgccgaa gtcgtctcc aaagagctg acacgagag 
tcggttgtt attttcgat gaggctcac aatatcgat 
aatgtttgc atcgactct atgagtgtg aaaatcaac 
aaacgaatt atcgatcga gcttccgcc aatattcag 
ttattggaa cgtactgtg gccgagatg cgtgaagac 
gatcacaaa aaattgcaa gaagaatat cagagatta 
gttgagggg ttgcgcgac gctagcgtg gcgcgagac 
actgacgtc attttggcc aatccggtc ttgcccgac 
gccatcctc caggaggcc gtccctgga aatattcgc 
aacgccgag cactttgtc agtttcctg aaacgtttc 
ctcgaatac ctcaaaact cggctgcga gtccagcac 
gtggtgcaa gaaagccct gctggattt ttaaaagac 
gtccaatcc aaagtctgt attgaacga aaaccgtta 
cgtttctgt gccgaacgc ttggcatct ctgttgaga 
actttggaa atatcagac ttgaccgac ttcagtccg 
gtcatttta gtcacacat ttggccact ttggtggcc 
acttacacg aaaggtttt acgataatc gtagaaccg 
tttgatgat aagacaccg acggtgtca aacccgatc 
ttacatttc agttgtttg gattcgtcg attgccata 
aaaccggtt tttgatcgg tttcaaacc gtggttata 
acctctgga accctatcc cctctagat atgtacccc 
aagattttg aacttccat cctgtgatc atgtcatcg 
ttcactatg acgttggcc aggccttgt ttgctacca 
atgatcgtg tcaaaaggc aacgaccaa gtcgcaatt 
tcgtcaaaa ttcgagaca cgcgaagac aatgctgtt 
atccgaaac tacggccaa cttctggtc gaaatagcc 
gccaatgtc cccgacgga atcgtctgt ttcttcacc 
tcttatctc tacctggaa tcggtcgtg gccagttgg 
tacgaccaa ggcgtcatc gacagcctc caaagatac 
aaactcctt ttcatcgag acccaggac tcggcagaa 
acgagcttc gccctcatg tactacatc aaagcttgc 
gagtcgggc cgcggggcc gtgctgctc tccgtcgcg 
cggggcaaa gtctccgaa ggggtcgac ttcgaccac 
cacctgggg cgcgcagtc ctcatgttc ggcattccc 
tacgtgtac acccagagc cgcatcctc aaagcgcgc 
ctcgactac cttcgggac cagttccag atccgggag 
aacgatttt ttgaccttt gacgccatg agacacgcg 
gcccagtgc gtggggagg gctatcagg gggaagacc 
gactacggt atcatgatt ttcgccgat aagaggttt 
tccaggtcg gataagcgg tcaaagttg cccaagtgg 
atccaggag catttgaag gactctctg acgaatctg 
tcgacggag gaggcggtc cagattgcc aagcggtgg 
ttgcgacag atggcgcag ccgttcact agggaggac 
cagttgggg gtgtctttg ctcactttg gagcagttg 
cagaatttg gaggcgagt aaaatccaa gagcaggcg 
caacaaaat tga
    mRNA sequence
    • >TC002825 RA
      ggttccagc tcacatgac ttaaatttt taggccccc 
ttcttcctt actaatcga ttagcaaca ataaaaacc 
tttctattg ttagaaaat attacaaaa aattgcata 
accgcactt ttttccaaa catgccata acctaaaaa 
aactaccgg gtcaatgta aacaccacg gtttcccca 
agcacctca caaaaaatg aaactaaac gtcgatggt 
ttaatcgtg tacttcccc tacgactac atctacccc 
gagcagtat gcctacatg tgcgagctc aagcgggcc 
ctggacgcc aaaggccac tgccttttg gaaatgccc 
tcagggacg ggtaagacc accactatc ctctccctc 
gttgttgcg tacatgctc gagcacccc catgacgtc 
cgcaaattg atctactgc tctcgtact gtccccgaa 
atcgagaaa gttatggaa gaattgaaa aaactgctc 
gagtattat gagaagttg gacggggag tacccccat 
ctcctgggg ctggttttg tcctcacgg aagaatctg 
tgtattcac ccggaagtg agtactgag cgcgagggt 
aagattgtg gacggaaaa tgccacgca ctaacagcg 
agttacata agggagagg cacaattat gatgataca 
actcccatt tgtcagtat tacgaaggt ttcgctttg 
gatggcaaa gaaagtacg atgccgttt ggggtttac 
agtatagat gatttgaaa cagtacgga cgggaccgc 
aactggtgc ccctatttc ctgtctcgt tttgtgata 
agttacgcg aatatcgta gtctacagt taccactat 
ttactggac cccaaaatc gccgaagtc gtctccaaa 
gagctgaca cgagagtcg gttgttatt ttcgatgag 
gctcacaat atcgataat gtttgcatc gactctatg 
agtgtgaaa atcaacaaa cgaattatc gatcgagct 
tccgccaat attcagtta ttggaacgt actgtggcc 
gagatgcgt gaagacgat cacaaaaaa ttgcaagaa 
gaatatcag agattagtt gaggggttg cgcgacgct 
agcgtggcg cgagacact gacgtcatt ttggccaat 
ccggtcttg cccgacgcc atcctccag gaggccgtc 
cctggaaat attcgcaac gccgagcac tttgtcagt 
ttcctgaaa cgtttcctc gaatacctc aaaactcgg 
ctgcgagtc cagcacgtg gtgcaagaa agccctgct 
ggattttta aaagacgtc caatccaaa gtctgtatt 
gaacgaaaa ccgttacgt ttctgtgcc gaacgcttg 
gcatctctg ttgagaact ttggaaata tcagacttg 
accgacttc agtccggtc attttagtc acacatttg 
gccactttg gtggccact tacacgaaa ggttttacg 
ataatcgta gaaccgttt gatgataag acaccgacg 
gtgtcaaac ccgatctta catttcagt tgtttggat 
tcgtcgatt gccataaaa ccggttttt gatcggttt 
caaaccgtg gttataacc tctggaacc ctatcccct 
ctagatatg taccccaag attttgaac ttccatcct 
gtgatcatg tcatcgttc actatgacg ttggccagg 
ccttgtttg ctaccaatg atcgtgtca aaaggcaac 
gaccaagtc gcaatttcg tcaaaattc gagacacgc 
gaagacaat gctgttatc cgaaactac ggccaactt 
ctggtcgaa atagccgcc aatgtcccc gacggaatc 
gtctgtttc ttcacctct tatctctac ctggaatcg 
gtcgtggcc agttggtac gaccaaggc gtcatcgac 
agcctccaa agatacaaa ctccttttc atcgagacc 
caggactcg gcagaaacg agcttcgcc ctcatgtac 
tacatcaaa gcttgcgag tcgggccgc ggggccgtg 
ctgctctcc gtcgcgcgg ggcaaagtc tccgaaggg 
gtcgacttc gaccaccac ctggggcgc gcagtcctc 
atgttcggc attccctac gtgtacacc cagagccgc 
atcctcaaa gcgcgcctc gactacctt cgggaccag 
ttccagatc cgggagaac gattttttg acctttgac 
gccatgaga cacgcggcc cagtgcgtg gggagggct 
atcaggggg aagaccgac tacggtatc atgattttc 
gccgataag aggttttcc aggtcggat aagcggtca 
aagttgccc aagtggatc caggagcat ttgaaggac 
tctctgacg aatctgtcg acggaggag gcggtccag 
attgccaag cggtggttg cgacagatg gcgcagccg 
ttcactagg gaggaccag ttgggggtg tctttgctc 
actttggag cagttgcag aatttggag gcgagtaaa 
atccaagag caggcgcaa caaaattga tttgttatt 
attttttac gtaataaag tattttcaa gaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY
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