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Gene information
General information
TC002825 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 4266838..4272761
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002825
    iBeetle screen iB_03457
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002825 RA
      MKLNVDGLI VYFPYDYIY PEQYAYMCE LKRALDAKG 
      HCLLEMPSG TGKTTTILS LVVAYMLEH PHDVRKLIY 
      CSRTVPEIE KVMEELKKL LEYYEKLDG EYPHLLGLV 
      LSSRKNLCI HPEVSTERE GKIVDGKCH ALTASYIRE 
      RHNYDDTTP ICQYYEGFA LDGKESTMP FGVYSIDDL 
      KQYGRDRNW CPYFLSRFV ISYANIVVY SYHYLLDPK 
      IAEVVSKEL TRESVVIFD EAHNIDNVC IDSMSVKIN 
      KRIIDRASA NIQLLERTV AEMREDDHK KLQEEYQRL 
      VEGLRDASV ARDTDVILA NPVLPDAIL QEAVPGNIR 
      NAEHFVSFL KRFLEYLKT RLRVQHVVQ ESPAGFLKD 
      VQSKVCIER KPLRFCAER LASLLRTLE ISDLTDFSP 
      VILVTHLAT LVATYTKGF TIIVEPFDD KTPTVSNPI 
      LHFSCLDSS IAIKPVFDR FQTVVITSG TLSPLDMYP 
      KILNFHPVI MSSFTMTLA RPCLLPMIV SKGNDQVAI 
      SSKFETRED NAVIRNYGQ LLVEIAANV PDGIVCFFT 
      SYLYLESVV ASWYDQGVI DSLQRYKLL FIETQDSAE 
      TSFALMYYI KACESGRGA VLLSVARGK VSEGVDFDH 
      HLGRAVLMF GIPYVYTQS RILKARLDY LRDQFQIRE 
      NDFLTFDAM RHAAQCVGR AIRGKTDYG IMIFADKRF 
      SRSDKRSKL PKWIQEHLK DSLTNLSTE EAVQIAKRW 
      LRQMAQPFT REDQLGVSL LTLEQLQNL EASKIQEQA 
      QQN
    CDS sequence
    • >TC002825 RA
      atgaaacta aacgtcgat ggtttaatc gtgtacttc 
      ccctacgac tacatctac cccgagcag tatgcctac 
      atgtgcgag ctcaagcgg gccctggac gccaaaggc 
      cactgcctt ttggaaatg ccctcaggg acgggtaag 
      accaccact atcctctcc ctcgttgtt gcgtacatg 
      ctcgagcac ccccatgac gtccgcaaa ttgatctac 
      tgctctcgt actgtcccc gaaatcgag aaagttatg 
      gaagaattg aaaaaactg ctcgagtat tatgagaag 
      ttggacggg gagtacccc catctcctg gggctggtt 
      ttgtcctca cggaagaat ctgtgtatt cacccggaa 
      gtgagtact gagcgcgag ggtaagatt gtggacgga 
      aaatgccac gcactaaca gcgagttac ataagggag 
      aggcacaat tatgatgat acaactccc atttgtcag 
      tattacgaa ggtttcgct ttggatggc aaagaaagt 
      acgatgccg tttggggtt tacagtata gatgatttg 
      aaacagtac ggacgggac cgcaactgg tgcccctat 
      ttcctgtct cgttttgtg ataagttac gcgaatatc 
      gtagtctac agttaccac tatttactg gaccccaaa 
      atcgccgaa gtcgtctcc aaagagctg acacgagag 
      tcggttgtt attttcgat gaggctcac aatatcgat 
      aatgtttgc atcgactct atgagtgtg aaaatcaac 
      aaacgaatt atcgatcga gcttccgcc aatattcag 
      ttattggaa cgtactgtg gccgagatg cgtgaagac 
      gatcacaaa aaattgcaa gaagaatat cagagatta 
      gttgagggg ttgcgcgac gctagcgtg gcgcgagac 
      actgacgtc attttggcc aatccggtc ttgcccgac 
      gccatcctc caggaggcc gtccctgga aatattcgc 
      aacgccgag cactttgtc agtttcctg aaacgtttc 
      ctcgaatac ctcaaaact cggctgcga gtccagcac 
      gtggtgcaa gaaagccct gctggattt ttaaaagac 
      gtccaatcc aaagtctgt attgaacga aaaccgtta 
      cgtttctgt gccgaacgc ttggcatct ctgttgaga 
      actttggaa atatcagac ttgaccgac ttcagtccg 
      gtcatttta gtcacacat ttggccact ttggtggcc 
      acttacacg aaaggtttt acgataatc gtagaaccg 
      tttgatgat aagacaccg acggtgtca aacccgatc 
      ttacatttc agttgtttg gattcgtcg attgccata 
      aaaccggtt tttgatcgg tttcaaacc gtggttata 
      acctctgga accctatcc cctctagat atgtacccc 
      aagattttg aacttccat cctgtgatc atgtcatcg 
      ttcactatg acgttggcc aggccttgt ttgctacca 
      atgatcgtg tcaaaaggc aacgaccaa gtcgcaatt 
      tcgtcaaaa ttcgagaca cgcgaagac aatgctgtt 
      atccgaaac tacggccaa cttctggtc gaaatagcc 
      gccaatgtc cccgacgga atcgtctgt ttcttcacc 
      tcttatctc tacctggaa tcggtcgtg gccagttgg 
      tacgaccaa ggcgtcatc gacagcctc caaagatac 
      aaactcctt ttcatcgag acccaggac tcggcagaa 
      acgagcttc gccctcatg tactacatc aaagcttgc 
      gagtcgggc cgcggggcc gtgctgctc tccgtcgcg 
      cggggcaaa gtctccgaa ggggtcgac ttcgaccac 
      cacctgggg cgcgcagtc ctcatgttc ggcattccc 
      tacgtgtac acccagagc cgcatcctc aaagcgcgc 
      ctcgactac cttcgggac cagttccag atccgggag 
      aacgatttt ttgaccttt gacgccatg agacacgcg 
      gcccagtgc gtggggagg gctatcagg gggaagacc 
      gactacggt atcatgatt ttcgccgat aagaggttt 
      tccaggtcg gataagcgg tcaaagttg cccaagtgg 
      atccaggag catttgaag gactctctg acgaatctg 
      tcgacggag gaggcggtc cagattgcc aagcggtgg 
      ttgcgacag atggcgcag ccgttcact agggaggac 
      cagttgggg gtgtctttg ctcactttg gagcagttg 
      cagaatttg gaggcgagt aaaatccaa gagcaggcg 
      caacaaaat tga
    mRNA sequence
    • >TC002825 RA
      ggttccagc tcacatgac ttaaatttt taggccccc 
      ttcttcctt actaatcga ttagcaaca ataaaaacc 
      tttctattg ttagaaaat attacaaaa aattgcata 
      accgcactt ttttccaaa catgccata acctaaaaa 
      aactaccgg gtcaatgta aacaccacg gtttcccca 
      agcacctca caaaaaatg aaactaaac gtcgatggt 
      ttaatcgtg tacttcccc tacgactac atctacccc 
      gagcagtat gcctacatg tgcgagctc aagcgggcc 
      ctggacgcc aaaggccac tgccttttg gaaatgccc 
      tcagggacg ggtaagacc accactatc ctctccctc 
      gttgttgcg tacatgctc gagcacccc catgacgtc 
      cgcaaattg atctactgc tctcgtact gtccccgaa 
      atcgagaaa gttatggaa gaattgaaa aaactgctc 
      gagtattat gagaagttg gacggggag tacccccat 
      ctcctgggg ctggttttg tcctcacgg aagaatctg 
      tgtattcac ccggaagtg agtactgag cgcgagggt 
      aagattgtg gacggaaaa tgccacgca ctaacagcg 
      agttacata agggagagg cacaattat gatgataca 
      actcccatt tgtcagtat tacgaaggt ttcgctttg 
      gatggcaaa gaaagtacg atgccgttt ggggtttac 
      agtatagat gatttgaaa cagtacgga cgggaccgc 
      aactggtgc ccctatttc ctgtctcgt tttgtgata 
      agttacgcg aatatcgta gtctacagt taccactat 
      ttactggac cccaaaatc gccgaagtc gtctccaaa 
      gagctgaca cgagagtcg gttgttatt ttcgatgag 
      gctcacaat atcgataat gtttgcatc gactctatg 
      agtgtgaaa atcaacaaa cgaattatc gatcgagct 
      tccgccaat attcagtta ttggaacgt actgtggcc 
      gagatgcgt gaagacgat cacaaaaaa ttgcaagaa 
      gaatatcag agattagtt gaggggttg cgcgacgct 
      agcgtggcg cgagacact gacgtcatt ttggccaat 
      ccggtcttg cccgacgcc atcctccag gaggccgtc 
      cctggaaat attcgcaac gccgagcac tttgtcagt 
      ttcctgaaa cgtttcctc gaatacctc aaaactcgg 
      ctgcgagtc cagcacgtg gtgcaagaa agccctgct 
      ggattttta aaagacgtc caatccaaa gtctgtatt 
      gaacgaaaa ccgttacgt ttctgtgcc gaacgcttg 
      gcatctctg ttgagaact ttggaaata tcagacttg 
      accgacttc agtccggtc attttagtc acacatttg 
      gccactttg gtggccact tacacgaaa ggttttacg 
      ataatcgta gaaccgttt gatgataag acaccgacg 
      gtgtcaaac ccgatctta catttcagt tgtttggat 
      tcgtcgatt gccataaaa ccggttttt gatcggttt 
      caaaccgtg gttataacc tctggaacc ctatcccct 
      ctagatatg taccccaag attttgaac ttccatcct 
      gtgatcatg tcatcgttc actatgacg ttggccagg 
      ccttgtttg ctaccaatg atcgtgtca aaaggcaac 
      gaccaagtc gcaatttcg tcaaaattc gagacacgc 
      gaagacaat gctgttatc cgaaactac ggccaactt 
      ctggtcgaa atagccgcc aatgtcccc gacggaatc 
      gtctgtttc ttcacctct tatctctac ctggaatcg 
      gtcgtggcc agttggtac gaccaaggc gtcatcgac 
      agcctccaa agatacaaa ctccttttc atcgagacc 
      caggactcg gcagaaacg agcttcgcc ctcatgtac 
      tacatcaaa gcttgcgag tcgggccgc ggggccgtg 
      ctgctctcc gtcgcgcgg ggcaaagtc tccgaaggg 
      gtcgacttc gaccaccac ctggggcgc gcagtcctc 
      atgttcggc attccctac gtgtacacc cagagccgc 
      atcctcaaa gcgcgcctc gactacctt cgggaccag 
      ttccagatc cgggagaac gattttttg acctttgac 
      gccatgaga cacgcggcc cagtgcgtg gggagggct 
      atcaggggg aagaccgac tacggtatc atgattttc 
      gccgataag aggttttcc aggtcggat aagcggtca 
      aagttgccc aagtggatc caggagcat ttgaaggac 
      tctctgacg aatctgtcg acggaggag gcggtccag 
      attgccaag cggtggttg cgacagatg gcgcagccg 
      ttcactagg gaggaccag ttgggggtg tctttgctc 
      actttggag cagttgcag aatttggag gcgagtaaa 
      atccaagag caggcgcaa caaaattga tttgttatt 
      attttttac gtaataaag tattttcaa gaaa