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Gene information
General information
TC002814 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 9183102..9191012
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002814
    iBeetle screen iB_03455

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 larval musculature partially randomnot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: larvae dead --> muscle defect probably secundary effect
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    remarks: animals lost, adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002814 RA
      MKEQQLSEV SGSNQLAPV LHQSINIQQ HLHHLHLHQ 
      NHTSVILPN EQTQQHPES TAPDDKPSS SDKKSGIRR 
      QEKPPYSYI ALIVMAIQH SPTKRLTLS EIYSFLQQR 
      FPFFRGSYQ GWKNSVRHN LSLNECFIK LPKGLGRPG 
      KGHYWTIDP ASEFMFEEG SFRRRPRGF RRKCQAMKP 
      QYHPGNFFT NNMTGMVNQ TPSYDPQTL VQPQEYSTC 
      SYNTSSPQV SSATHYTNY DYPVYQQQC DRDWGSASA 
      LLPPYPETA ISSSYLKAP ISPLGDNQE TPPPGLSDA 
      YNYQYGGIS TSSADNLMR PSTNTMQPI QCDRKPYLS 
      SPPPSIPSS LPSPPAANT AHSFYEPTV KYSL
    CDS sequence
    • >TC002814 RA
      atgaaggag caacagttg tctgaagtg agcggctct 
      aaccaactc gctccggtg ctccaccag agcatcaac 
      atccagcag cacctacac cacctgcac ctgcaccag 
      aaccacacc agtgtcatt ctacccaat gagcaaacg 
      cagcaacac ccggagtcc accgctccg gacgacaag 
      ccctccagc tccgacaag aagtccggg atccggagg 
      caggagaaa ccgccctat tcctacatc gcgctcatc 
      gtcatggcc atacaacac tcgcccacc aaacggctc 
      acgctcagc gagatctac tctttcctc cagcagagg 
      ttcccgttt ttccgagga tcttaccaa ggatggaaa 
      aactcggtc cgacacaac ttgtccctc aacgagtgt 
      tttatcaag ttaccaaag ggcctgggg cgccctggc 
      aagggccac tactggacc atcgaccca gcgagcgag 
      ttcatgttc gaggagggc tccttccgg aggcgccct 
      cgcggcttc cgtcggaag tgccaagcg atgaagccc 
      caataccac cctggaaac tttttcacg aacaacatg 
      accggaatg gtgaatcaa acccccagc tacgacccc 
      caaaccctc gtccagccc caggagtac tccacttgc 
      tcctacaac acctcttcg ccgcaagtc tcctccgcc 
      acgcattac accaactac gactacccc gtgtaccag 
      cagcagtgt gaccgcgac tggggctcg gcctccgcc 
      ctgctgccc ccatacccc gaaacggcc atctccagc 
      tcgtacctc aaggccccc atcagcccg ctgggggac 
      aaccaggag acgccgccg ccggggctc tccgacgcc 
      tataattat cagtacgga ggcatttcg acctcatct 
      gcagataat ttgatgaga ccttccaca aacaccatg 
      cagccaatt cagtgcgac cgaaagccg tacttgagt 
      tcgccgccg ccttcgatt ccatcatct cttccatca 
      ccaccagca gcgaacaca gcacactca ttttacgaa 
      cctacagta aaatacagc ttataa
    mRNA sequence
    • >TC002814 RA
      cggggctcg gtggggaca tcccacaca cacatccac 
      atgcaccaa gacccgcct gaattagtt tcctcgctc 
      gagtctcga tcgacgtgc atcactatc gaaatcgcg 
      gtttcgtcg gggctttca gagcacgac tgaccgtct 
      acgtgtccg cacctcgac cattttaat atcactgaa 
      atttactgc tccgacatt tgtatataa aacgcgtaa 
      attgtgtac gatttcgtc gcgtgtttg tatataaac 
      tgtgtatac aatatttgt gtatacaac tcgttacga 
      gtatcaaat gtttgtaat ataaagacg gagattaaa 
      ttgtttagt ttaccgcgt aaatgttta aagtatgat 
      gaaggagca acagttgtc tgaagtgag cggctctaa 
      ccaactcgc tccggtgct ccaccagag catcaacat 
      ccagcagca cctacacca cctgcacct gcaccagaa 
      ccacaccag tgtcattct acccaatga gcaaacgca 
      gcaacaccc ggagtccac cgctccgga cgacaagcc 
      ctccagctc cgacaagaa gtccgggat ccggaggca 
      ggagaaacc gccctattc ctacatcgc gctcatcgt 
      catggccat acaacactc gcccaccaa acggctcac 
      gctcagcga gatctactc tttcctcca gcagaggtt 
      cccgttttt ccgaggatc ttaccaagg atggaaaaa 
      ctcggtccg acacaactt gtccctcaa cgagtgttt 
      tatcaagtt accaaaggg cctggggcg ccctggcaa 
      gggccacta ctggaccat cgacccagc gagcgagtt 
      catgttcga ggagggctc cttccggag gcgccctcg 
      cggcttccg tcggaagtg ccaagcgat gaagcccca 
      ataccaccc tggaaactt tttcacgaa caacatgac 
      cggaatggt gaatcaaac ccccagcta cgaccccca 
      aaccctcgt ccagcccca ggagtactc cacttgctc 
      ctacaacac ctcttcgcc gcaagtctc ctccgccac 
      gcattacac caactacga ctaccccgt gtaccagca 
      gcagtgtga ccgcgactg gggctcggc ctccgccct 
      gctgccccc ataccccga aacggccat ctccagctc 
      gtacctcaa ggcccccat cagcccgct gggggacaa 
      ccaggagac gccgccgcc ggggctctc cgacgccta 
      taattatca gtacggagg catttcgac ctcatctgc 
      agataattt gatgagacc ttccacaaa caccatgca 
      gccaattca gtgcgaccg aaagccgta cttgagttc 
      gccgccgcc ttcgattcc atcatctct tccatcacc 
      accagcagc gaacacagc acactcatt ttacgaacc 
      tacagtaaa atacagctt ataaattca gtatttatg 
      gtaactttt aagtggaca tttgtactt gtcatttat 
      ccgaattaa ttttatgtc tctttcgga attcctctt 
      caatcgtgc aaacgcagc tcaattttg cacaattgt 
      acatatatt tattttcta ggtttagtg cctactttt 
      aagtaagac gtaatcatg atttaacat gtatataaa 
      cacttcata cagctactt accaaaaat gatgtacat 
      aaaacgatt tgattttgt aatttatga taaaaataa 
      aattttgat tttact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY