Status
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Gene information
General information
TC002804 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 9637541..9642639
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002804
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03454

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs segment shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs segment potentiallyfused - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002804 RA
      MARLILICV IVSAYLGGI FSQEKTEVV SNTEDPSLS 
      NSILKGATK SGTLFDYQT RVVAYKDLI ESRCLIEFL 
      KASESYIGQ SNHKPRLDN VTKNFFMTE NQLSKRQIW 
      TIAGQRVVD FCRGFPTYL IHDLKTNDQ NSIFQDSLN 
      EGIAARKKR ATKHFFVGR VRGQTQSQY LNYGNSKDP 
      GKAEAESSY GSSKAVVSG SSGMGQAQS QSVPVGCDE 
      CYGEGYDQE QGLYYGRPG GTPEFPGEQ YRKPGRPSI 
      PGYPAEPGG RPGVPGYPR GPEERPGYP GGPAVPGYP 
      GLPGGRPGI PEYPGAPGG RPAIPGYPG GPEGRPEIP 
      GYPGKPEER PGVPGFPGR PDGRPGVPG YPGRPGERP 
      EIPGYPHIP EGRPRIPGY PGRPEERPA YPEGPVEKP 
      GIPGYPEGP EGRPGFPGR PEERPVVPG YPSLPGGRP 
      GVPGYPRGP DGRPAFPGY PQPGVPGYS KQPGQLPGV 
      PGVPGYPGE PEAKPGVPG YPRPGVPGY PGGEIPSHP 
      GGEKPGYPL SPTGPGVPG YPGYPGYPQ YPTGPSPGL 
      PEGPSRPGY PSGPEKPGY PPTEGYPLR PGYPTPPSY 
      PVPGEVGYP TPPGKVSTG KFSGQLSGD YEAEVGQRG 
      KFSGIFSGQ YEAHHNGKI AGKYVDGKY YPGQPAGPS 
      YPGYPGLPT PPGYPGPAP YPTAPGGVG KITYPVGPG 
      GVPGYPGTP TYPSIPGYP TPGHPVTEP GYPGVPSYP 
      GAPRPGVPS GPSGPSYPG GQIPGYPGV PSPGFPGGP 
      PPGYPGVPT AGYPGIPTG PGVPSYPGG PSVSFPGVP 
      SVPPGVPSY PGAPSPGYP GAPSSGYPG APSPGYPGA 
      PSPGYPGAP SPGYPGAPY PGYPEAPTP SYPGAPTPG 
      YPGAPSPGY PGAPAPGYP EAPYPGYPG VPSPGYPGA 
      PSPGYPGVP SGPGIPSYP QGPPPGAPG YPGVPTGPS 
      YPGAPGGPG YPEPRPGQP GYPGVPPGY PGAPGETPS 
      RLTYPGAPG QPGYPGAPT GEEADGADS QVQTSVQQT 
      NNETQASAQ AQGRYAGGM AQSQVSGTY SGSGSFSAS 
      AGSDDGKKG ALSQVSGGK EGALSSAQG RGGLGQSQA 
      QVQLASESG DTISSAQTD GWNHGSQTQ VQASGKGGM 
      ADAQANGPG STSSQAQIG FTPYDENER NDQSTPFRG 
      GGTASAQSG TYSGQAQSQ IQGKFQYGI KYTGAAQAG 
      SGSRDTIAL ARNQSRPEG LTGTFPKFT PLNFTSGRN 
      PNTNPQDLL MQTSRKSVH KTLSMDVAT QKAPTEDQT 
      VPIPDETED TATEDYHDE DYDYDSDYS TQKPITKSI 
      ISQSSKTQR QHIVLDPLE DLDATVQQS YGYPPDGTI 
      LQPGETIPG SPGYQIPAG FRGRVKAIA NGPNTYATG 
      RNSQAQSVT LTPGTGRII YKKPIYAVT SNSHQNGHM 
      GFGSGYTYQ PATYQVKSG KSLPNFISL SKSETGSKN 
      PYTGQKTPK VYYAQSSSC GVFTNTCVF NGSGKLCFP 
      KPKTNPDGS FMTC
    CDS sequence
    • >TC002804 RA
      atggcgcgg ttaatttta atttgtgtc atagtgagt 
      gcttatcta ggtggcata ttttcgcag gaaaaaaca 
      gaggttgtt tctaatact gaagatcca tctctgtcc 
      aactcgata cttaaagga gcaacaaag tcgggaact 
      ttgtttgat taccaaact agagtagta gcttataaa 
      gatctcata gaatcaaga tgtttgata gaattttta 
      aaagctagc gaaagctac attggacag tcgaaccat 
      aaaccacgg ctggataat gtcaccaag aattttttt 
      atgactgaa aatcagctc tccaaaaga caaatatgg 
      acaatagct gggcaaaga gttgtagat ttttgtcgc 
      ggttttcca acttatctc atccacgat ttaaaaaca 
      aacgaccaa aacagtatt tttcaagac agtctaaac 
      gagggtatt gccgcaaga aaaaaacgt gcaactaaa 
      cattttttc gttggaagg gttagagga caaacacaa 
      tcacaatat ctaaattat ggtaacagc aaagatcct 
      ggaaaagct gaagccgaa tcaagttat ggaagttca 
      aaggcagta gtcagtggt agtagcgga atgggccaa 
      gcccagagt caaagtgtt ccagtgggt tgtgacgaa 
      tgttatggc gaaggttac gatcaagaa caagggctt 
      tattatgga agacctggg ggtacaccg gaatttccg 
      ggagaacaa tatcgcaag cctggaaga cctagcatt 
      ccgggatat ccagctgaa cctggagga agacccgga 
      gttcccgga tatcctaga gggccagaa gaaagaccc 
      ggatatcca ggaggaccc gcagttccc ggatatccg 
      ggattacca ggaggtaga cccggaatt cctgaatac 
      ccgggagca ccaggggga cgacctgca attcctgga 
      tatccagga ggaccagaa ggaagaccc gaaattcca 
      ggatatcca ggaaaacca gaagaaaga cctggcgtt 
      ccgggattt ccagggaga ccagatggg cggcctggt 
      gtgccagga tatccagga agaccagga gaacgacca 
      gaaataccc ggatatcct catatacct gagggaaga 
      ccaagaatt cctggatat ccaggaaga cccgaagaa 
      agacctgca tatccagag ggaccagta gaaaaacct 
      ggaattcct ggttatcct gagggacca gaaggtaga 
      cccggattt ccaggacga ccagaagaa agacctgta 
      gtccccggc tacccgagt ttaccaggg ggaagaccg 
      ggagtaccg ggttatcca agaggaccg gatggacgg 
      ccagcattt ccagggtat ccacaacct ggagttccc 
      ggatattca aaacaaccc ggacaacta cctggagtt 
      cccggagta ccaggatat cccggtgaa cccgaagca 
      aaacctggt gtaccagga taccctcga ccgggagtt 
      cctgggtac cctggggga gaaataccg tcgcatcca 
      ggaggagaa aagcccgga tatccgctt tcacctact 
      ggacctgga gtaccgggt taccctggt tatcctggc 
      tacccacag taccctaca gggccttct ccaggacta 
      ccagaaggt ccttcacgg cccggttat cctagcggt 
      ccggaaaag ccaggatat cctccaact gaaggctat 
      ccattaaga ccgggatat ccaacacca cccagctat 
      ccagtgccg ggagaagtg ggatatcct actccacct 
      ggaaaagtt tccaccggc aaatttagt ggacaactt 
      tccggagat tatgaagca gaagttgga caaaggggt 
      aaattttct ggaatcttt tccggacaa tatgaagct 
      catcataat ggaaaaatt gccggaaaa tatgttgat 
      ggaaaatac tatccaggc cagcctgca ggtcctagt 
      tacccggga tatcccggc ttaccaact ccaccaggt 
      tatcctgga cctgcacct tatcctact gcacccggc 
      ggcgtaggc aaaatcacg tatcccgta ggacctggt 
      ggagtaccg ggttaccct ggaacacca acttatcca 
      agcattccc ggatatcca acacccgga cacccagta 
      acagaaccg ggatatcca ggagtacca agttacccg 
      ggagcacca cgtccagga gtaccatcc ggcccaagc 
      ggtccaagt tatcccggt ggtcaaatc cccggctat 
      ccaggagta ccatcaccg ggtttccca ggaggtcca 
      cctccaggt tatccagga gttccaacg gcaggttat 
      ccaggaatt ccaactggc ccaggggtt ccaagttac 
      cctggaggt ccttctgtt agttttccc ggagtacca 
      tcagttcct ccaggagtt ccaagttat ccaggagct 
      ccatctcct ggctatcca ggagcccca tcttctggc 
      tatccagga gctccatcg cctggctat ccaggagct 
      ccatcgcct ggctatcca ggagctcca tcgcctggc 
      tatccagga gccccatat cctggttat ccagaagct 
      ccgactcca agctatcca ggagctcca acccctggt 
      tatccagga gctccgtct ccgggctat ccaggcgca 
      ccagccccg ggttaccca gaagcccca tatccgggc 
      tacccagga gttccatct cctggctat ccaggggct 
      ccatctcct ggttatccg ggcgtacca tcaggtcca 
      ggtattcca agttatcca caaggtcca cctccaggt 
      gctcctggt tatccagga gttccaaca ggtccaagt 
      tatcccgga gcgccagga ggacccggc taccccgaa 
      ccaagacca ggtcaacca ggttatcca ggagtacca 
      cccggttat cccggagcc ccaggcgaa actccaagt 
      aggctcact tatcctgga gctcctggc caacccgga 
      tatcccggt gcaccaacg ggagaagaa gccgacggt 
      gccgactct caagtacaa acatccgtc caacaaacc 
      aataacgaa acacaagca agtgctcaa gctcaagga 
      cgatacgct ggaggaatg gcccagtct caagtttcc 
      ggtacttac agcggttcc ggttctttc agtgcttca 
      gctggaagt gatgatggc aaaaaaggg gcattatca 
      caagtttcc gggggtaag gaaggtgca ttgagctca 
      gctcaagga agaggcggc ttgggtcaa agtcaagca 
      caagttcaa ctagcatca gaaagtggt gataccata 
      tcaagtgct cagacggat ggatggaac cacggttcg 
      caaacacag gtacaagca agtgggaaa ggaggaatg 
      gcagatgct caagcgaac ggacctgga agcacctcg 
      tctcaggct caaattggg ttcactccg tatgacgaa 
      aacgaaaga aacgaccaa tcgacgcct tttagaggc 
      ggaggaaca gcaagtgcc caaagtggt acttactca 
      ggacaagcc caaagtcaa atccagggg aagtttcaa 
      tacggcatt aaatacacg ggagctgca caagccggt 
      tcagggagc agagacaca atagctttg gctcggaac 
      cagtcaaga cctgaagga cttacagga accttccca 
      aaattcaca cctttgaat ttcacttct gggcgaaac 
      ccaaacact aatccacaa gacttactg atgcaaact 
      agccgaaaa tcagttcat aaaacacta agtatggac 
      gtagcaacc caaaaggca ccaactgaa gaccaaact 
      gttccaatc cctgacgaa accgaagac acagctaca 
      gaagactac catgacgaa gactacgac tacgactca 
      gactactcc acccaaaaa cccataacc aaaagcata 
      atctcccaa tcctccaaa acgcaacgc cagcacatc 
      gtgctcgac cctctcgaa gacctcgat gccacagtg 
      caacaaagc tacgggtac cctcccgat ggtaccatc 
      ctccaacct ggagaaacg attcctggc tctcccggc 
      taccagatc cctgccggg ttccgggga cgtgtcaag 
      gccatcgct aacgggccg aacacctac gccacgggc 
      agaaactcg caagctcag agtgtgaca ctgacacca 
      gggactgga cgcattatt tacaaaaaa ccgatctat 
      gcagtcacc tctaattcg caccaaaac ggacatatg 
      ggcttcggg tcgggatat acttatcaa ccggcgacg 
      tatcaagtc aagagcggg aaatctcta ccgaatttt 
      atttccctg agcaagtcg gaaacaggg tctaagaac 
      ccttacact ggacaaaag accccgaag gtgtattat 
      gcccagtct tcgagttgt ggggttttc acgaatact 
      tgcgttttt aatggaagt ggaaaactg tgctttccc 
      aagcccaag actaacccc gatgggagt tttatgacg 
      tgttga
    mRNA sequence
    • >TC002804 RA
      tatcttcta ggggacacc atgtcaggg gtcacactt 
      gaccaggaa tagattcag catttattg ataggccgc 
      cctgcggtc acttactcc catggcgcg gttaatttt 
      aatttgtgt catagtgag tgcttatct aggtggcat 
      attttcgca ggaaaaaac agaggttgt ttctaatac 
      tgaagatcc atctctgtc caactcgat acttaaagg 
      agcaacaaa gtcgggaac tttgtttga ttaccaaac 
      tagagtagt agcttataa agatctcat agaatcaag 
      atgtttgat agaattttt aaaagctag cgaaagcta 
      cattggaca gtcgaacca taaaccacg gctggataa 
      tgtcaccaa gaatttttt tatgactga aaatcagct 
      ctccaaaag acaaatatg gacaatagc tgggcaaag 
      agttgtaga tttttgtcg cggttttcc aacttatct 
      catccacga tttaaaaac aaacgacca aaacagtat 
      ttttcaaga cagtctaaa cgagggtat tgccgcaag 
      aaaaaaacg tgcaactaa acatttttt cgttggaag 
      ggttagagg acaaacaca atcacaata tctaaatta 
      tggtaacag caaagatcc tggaaaagc tgaagccga 
      atcaagtta tggaagttc aaaggcagt agtcagtgg 
      tagtagcgg aatgggcca agcccagag tcaaagtgt 
      tccagtggg ttgtgacga atgttatgg cgaaggtta 
      cgatcaaga acaagggct ttattatgg aagacctgg 
      gggtacacc ggaatttcc gggagaaca atatcgcaa 
      gcctggaag acctagcat tccgggata tccagctga 
      acctggagg aagacccgg agttcccgg atatcctag 
      agggccaga agaaagacc cggatatcc aggaggacc 
      cgcagttcc cggatatcc gggattacc aggaggtag 
      acccggaat tcctgaata cccgggagc accaggggg 
      acgacctgc aattcctgg atatccagg aggaccaga 
      aggaagacc cgaaattcc aggatatcc aggaaaacc 
      agaagaaag acctggcgt tccgggatt tccagggag 
      accagatgg gcggcctgg tgtgccagg atatccagg 
      aagaccagg agaacgacc agaaatacc cggatatcc 
      tcatatacc tgagggaag accaagaat tcctggata 
      tccaggaag acccgaaga aagacctgc atatccaga 
      gggaccagt agaaaaacc tggaattcc tggttatcc 
      tgagggacc agaaggtag acccggatt tccaggacg 
      accagaaga aagacctgt agtccccgg ctacccgag 
      tttaccagg gggaagacc gggagtacc gggttatcc 
      aagaggacc ggatggacg gccagcatt tccagggta 
      tccacaacc tggagttcc cggatattc aaaacaacc 
      cggacaact acctggagt tcccggagt accaggata 
      tcccggtga acccgaagc aaaacctgg tgtaccagg 
      ataccctcg accgggagt tcctgggta ccctggggg 
      agaaatacc gtcgcatcc aggaggaga aaagcccgg 
      atatccgct ttcacctac tggacctgg agtaccggg 
      ttaccctgg ttatcctgg ctacccaca gtaccctac 
      agggccttc tccaggact accagaagg tccttcacg 
      gcccggtta tcctagcgg tccggaaaa gccaggata 
      tcctccaac tgaaggcta tccattaag accgggata 
      tccaacacc acccagcta tccagtgcc gggagaagt 
      gggatatcc tactccacc tggaaaagt ttccaccgg 
      caaatttag tggacaact ttccggaga ttatgaagc 
      agaagttgg acaaagggg taaattttc tggaatctt 
      ttccggaca atatgaagc tcatcataa tggaaaaat 
      tgccggaaa atatgttga tggaaaata ctatccagg 
      ccagcctgc aggtcctag ttacccggg atatcccgg 
      cttaccaac tccaccagg ttatcctgg acctgcacc 
      ttatcctac tgcacccgg cggcgtagg caaaatcac 
      gtatcccgt aggacctgg tggagtacc gggttaccc 
      tggaacacc aacttatcc aagcattcc cggatatcc 
      aacacccgg acacccagt aacagaacc gggatatcc 
      aggagtacc aagttaccc gggagcacc acgtccagg 
      agtaccatc cggcccaag cggtccaag ttatcccgg 
      tggtcaaat ccccggcta tccaggagt accatcacc 
      gggtttccc aggaggtcc acctccagg ttatccagg 
      agttccaac ggcaggtta tccaggaat tccaactgg 
      cccaggggt tccaagtta ccctggagg tccttctgt 
      tagttttcc cggagtacc atcagttcc tccaggagt 
      tccaagtta tccaggagc tccatctcc tggctatcc 
      aggagcccc atcttctgg ctatccagg agctccatc 
      gcctggcta tccaggagc tccatcgcc tggctatcc 
      aggagctcc atcgcctgg ctatccagg agccccata 
      tcctggtta tccagaagc tccgactcc aagctatcc 
      aggagctcc aacccctgg ttatccagg agctccgtc 
      tccgggcta tccaggcgc accagcccc gggttaccc 
      agaagcccc atatccggg ctacccagg agttccatc 
      tcctggcta tccaggggc tccatctcc tggttatcc 
      gggcgtacc atcaggtcc aggtattcc aagttatcc 
      acaaggtcc acctccagg tgctcctgg ttatccagg 
      agttccaac aggtccaag ttatcccgg agcgccagg 
      aggacccgg ctaccccga accaagacc aggtcaacc 
      aggttatcc aggagtacc acccggtta tcccggagc 
      cccaggcga aactccaag taggctcac ttatcctgg 
      agctcctgg ccaacccgg atatcccgg tgcaccaac 
      gggagaaga agccgacgg tgccgactc tcaagtaca 
      aacatccgt ccaacaaac caataacga aacacaagc 
      aagtgctca agctcaagg acgatacgc tggaggaat 
      ggcccagtc tcaagtttc cggtactta cagcggttc 
      cggttcttt cagtgcttc agctggaag tgatgatgg 
      caaaaaagg ggcattatc acaagtttc cgggggtaa 
      ggaaggtgc attgagctc agctcaagg aagaggcgg 
      cttgggtca aagtcaagc acaagttca actagcatc 
      agaaagtgg tgataccat atcaagtgc tcagacgga 
      tggatggaa ccacggttc gcaaacaca ggtacaagc 
      aagtgggaa aggaggaat ggcagatgc tcaagcgaa 
      cggacctgg aagcacctc gtctcaggc tcaaattgg 
      gttcactcc gtatgacga aaacgaaag aaacgacca 
      atcgacgcc ttttagagg cggaggaac agcaagtgc 
      ccaaagtgg tacttactc aggacaagc ccaaagtca 
      aatccaggg gaagtttca atacggcat taaatacac 
      gggagctgc acaagccgg ttcagggag cagagacac 
      aatagcttt ggctcggaa ccagtcaag acctgaagg 
      acttacagg aaccttccc aaaattcac acctttgaa 
      tttcacttc tgggcgaaa cccaaacac taatccaca 
      agacttact gatgcaaac tagccgaaa atcagttca 
      taaaacact aagtatgga cgtagcaac ccaaaaggc 
      accaactga agaccaaac tgttccaat ccctgacga 
      aaccgaaga cacagctac agaagacta ccatgacga 
      agactacga ctacgactc agactactc cacccaaaa 
      acccataac caaaagcat aatctccca atcctccaa 
      aacgcaacg ccagcacat cgtgctcga ccctctcga 
      agacctcga tgccacagt gcaacaaag ctacgggta 
      ccctcccga tggtaccat cctccaacc tggagaaac 
      gattcctgg ctctcccgg ctaccagat ccctgccgg 
      gttccgggg acgtgtcaa ggccatcgc taacgggcc 
      gaacaccta cgccacggg cagaaactc gcaagctca 
      gagtgtgac actgacacc agggactgg acgcattat 
      ttacaaaaa accgatcta tgcagtcac ctctaattc 
      gcaccaaaa cggacatat gggcttcgg gtcgggata 
      tacttatca accggcgac gtatcaagt caagagcgg 
      gaaatctct accgaattt tatttccct gagcaagtc 
      ggaaacagg gtctaagaa cccttacac tggacaaaa 
      gaccccgaa ggtgtatta tgcccagtc ttcgagttg 
      tggggtttt cacgaatac ttgcgtttt taatggaag 
      tggaaaact gtgctttcc caagcccaa gactaaccc 
      cgatgggag ttttatgac gtgttgagg gttgtttgt 
      attgtaagt taattaagg ttttgtaaa ttatgtcgt 
      gtcataagt tttaaataa agtttttga ttattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY