Status
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Gene information
General information
TC002804 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 9637541..9642639
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002804
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03454

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs segment shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs segment potentiallyfused - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002804 RA
      MARLILICV IVSAYLGGI FSQEKTEVV SNTEDPSLS 
NSILKGATK SGTLFDYQT RVVAYKDLI ESRCLIEFL 
KASESYIGQ SNHKPRLDN VTKNFFMTE NQLSKRQIW 
TIAGQRVVD FCRGFPTYL IHDLKTNDQ NSIFQDSLN 
EGIAARKKR ATKHFFVGR VRGQTQSQY LNYGNSKDP 
GKAEAESSY GSSKAVVSG SSGMGQAQS QSVPVGCDE 
CYGEGYDQE QGLYYGRPG GTPEFPGEQ YRKPGRPSI 
PGYPAEPGG RPGVPGYPR GPEERPGYP GGPAVPGYP 
GLPGGRPGI PEYPGAPGG RPAIPGYPG GPEGRPEIP 
GYPGKPEER PGVPGFPGR PDGRPGVPG YPGRPGERP 
EIPGYPHIP EGRPRIPGY PGRPEERPA YPEGPVEKP 
GIPGYPEGP EGRPGFPGR PEERPVVPG YPSLPGGRP 
GVPGYPRGP DGRPAFPGY PQPGVPGYS KQPGQLPGV 
PGVPGYPGE PEAKPGVPG YPRPGVPGY PGGEIPSHP 
GGEKPGYPL SPTGPGVPG YPGYPGYPQ YPTGPSPGL 
PEGPSRPGY PSGPEKPGY PPTEGYPLR PGYPTPPSY 
PVPGEVGYP TPPGKVSTG KFSGQLSGD YEAEVGQRG 
KFSGIFSGQ YEAHHNGKI AGKYVDGKY YPGQPAGPS 
YPGYPGLPT PPGYPGPAP YPTAPGGVG KITYPVGPG 
GVPGYPGTP TYPSIPGYP TPGHPVTEP GYPGVPSYP 
GAPRPGVPS GPSGPSYPG GQIPGYPGV PSPGFPGGP 
PPGYPGVPT AGYPGIPTG PGVPSYPGG PSVSFPGVP 
SVPPGVPSY PGAPSPGYP GAPSSGYPG APSPGYPGA 
PSPGYPGAP SPGYPGAPY PGYPEAPTP SYPGAPTPG 
YPGAPSPGY PGAPAPGYP EAPYPGYPG VPSPGYPGA 
PSPGYPGVP SGPGIPSYP QGPPPGAPG YPGVPTGPS 
YPGAPGGPG YPEPRPGQP GYPGVPPGY PGAPGETPS 
RLTYPGAPG QPGYPGAPT GEEADGADS QVQTSVQQT 
NNETQASAQ AQGRYAGGM AQSQVSGTY SGSGSFSAS 
AGSDDGKKG ALSQVSGGK EGALSSAQG RGGLGQSQA 
QVQLASESG DTISSAQTD GWNHGSQTQ VQASGKGGM 
ADAQANGPG STSSQAQIG FTPYDENER NDQSTPFRG 
GGTASAQSG TYSGQAQSQ IQGKFQYGI KYTGAAQAG 
SGSRDTIAL ARNQSRPEG LTGTFPKFT PLNFTSGRN 
PNTNPQDLL MQTSRKSVH KTLSMDVAT QKAPTEDQT 
VPIPDETED TATEDYHDE DYDYDSDYS TQKPITKSI 
ISQSSKTQR QHIVLDPLE DLDATVQQS YGYPPDGTI 
LQPGETIPG SPGYQIPAG FRGRVKAIA NGPNTYATG 
RNSQAQSVT LTPGTGRII YKKPIYAVT SNSHQNGHM 
GFGSGYTYQ PATYQVKSG KSLPNFISL SKSETGSKN 
PYTGQKTPK VYYAQSSSC GVFTNTCVF NGSGKLCFP 
KPKTNPDGS FMTC
    CDS sequence
    • >TC002804 RA
      atggcgcgg ttaatttta atttgtgtc atagtgagt 
gcttatcta ggtggcata ttttcgcag gaaaaaaca 
gaggttgtt tctaatact gaagatcca tctctgtcc 
aactcgata cttaaagga gcaacaaag tcgggaact 
ttgtttgat taccaaact agagtagta gcttataaa 
gatctcata gaatcaaga tgtttgata gaattttta 
aaagctagc gaaagctac attggacag tcgaaccat 
aaaccacgg ctggataat gtcaccaag aattttttt 
atgactgaa aatcagctc tccaaaaga caaatatgg 
acaatagct gggcaaaga gttgtagat ttttgtcgc 
ggttttcca acttatctc atccacgat ttaaaaaca 
aacgaccaa aacagtatt tttcaagac agtctaaac 
gagggtatt gccgcaaga aaaaaacgt gcaactaaa 
cattttttc gttggaagg gttagagga caaacacaa 
tcacaatat ctaaattat ggtaacagc aaagatcct 
ggaaaagct gaagccgaa tcaagttat ggaagttca 
aaggcagta gtcagtggt agtagcgga atgggccaa 
gcccagagt caaagtgtt ccagtgggt tgtgacgaa 
tgttatggc gaaggttac gatcaagaa caagggctt 
tattatgga agacctggg ggtacaccg gaatttccg 
ggagaacaa tatcgcaag cctggaaga cctagcatt 
ccgggatat ccagctgaa cctggagga agacccgga 
gttcccgga tatcctaga gggccagaa gaaagaccc 
ggatatcca ggaggaccc gcagttccc ggatatccg 
ggattacca ggaggtaga cccggaatt cctgaatac 
ccgggagca ccaggggga cgacctgca attcctgga 
tatccagga ggaccagaa ggaagaccc gaaattcca 
ggatatcca ggaaaacca gaagaaaga cctggcgtt 
ccgggattt ccagggaga ccagatggg cggcctggt 
gtgccagga tatccagga agaccagga gaacgacca 
gaaataccc ggatatcct catatacct gagggaaga 
ccaagaatt cctggatat ccaggaaga cccgaagaa 
agacctgca tatccagag ggaccagta gaaaaacct 
ggaattcct ggttatcct gagggacca gaaggtaga 
cccggattt ccaggacga ccagaagaa agacctgta 
gtccccggc tacccgagt ttaccaggg ggaagaccg 
ggagtaccg ggttatcca agaggaccg gatggacgg 
ccagcattt ccagggtat ccacaacct ggagttccc 
ggatattca aaacaaccc ggacaacta cctggagtt 
cccggagta ccaggatat cccggtgaa cccgaagca 
aaacctggt gtaccagga taccctcga ccgggagtt 
cctgggtac cctggggga gaaataccg tcgcatcca 
ggaggagaa aagcccgga tatccgctt tcacctact 
ggacctgga gtaccgggt taccctggt tatcctggc 
tacccacag taccctaca gggccttct ccaggacta 
ccagaaggt ccttcacgg cccggttat cctagcggt 
ccggaaaag ccaggatat cctccaact gaaggctat 
ccattaaga ccgggatat ccaacacca cccagctat 
ccagtgccg ggagaagtg ggatatcct actccacct 
ggaaaagtt tccaccggc aaatttagt ggacaactt 
tccggagat tatgaagca gaagttgga caaaggggt 
aaattttct ggaatcttt tccggacaa tatgaagct 
catcataat ggaaaaatt gccggaaaa tatgttgat 
ggaaaatac tatccaggc cagcctgca ggtcctagt 
tacccggga tatcccggc ttaccaact ccaccaggt 
tatcctgga cctgcacct tatcctact gcacccggc 
ggcgtaggc aaaatcacg tatcccgta ggacctggt 
ggagtaccg ggttaccct ggaacacca acttatcca 
agcattccc ggatatcca acacccgga cacccagta 
acagaaccg ggatatcca ggagtacca agttacccg 
ggagcacca cgtccagga gtaccatcc ggcccaagc 
ggtccaagt tatcccggt ggtcaaatc cccggctat 
ccaggagta ccatcaccg ggtttccca ggaggtcca 
cctccaggt tatccagga gttccaacg gcaggttat 
ccaggaatt ccaactggc ccaggggtt ccaagttac 
cctggaggt ccttctgtt agttttccc ggagtacca 
tcagttcct ccaggagtt ccaagttat ccaggagct 
ccatctcct ggctatcca ggagcccca tcttctggc 
tatccagga gctccatcg cctggctat ccaggagct 
ccatcgcct ggctatcca ggagctcca tcgcctggc 
tatccagga gccccatat cctggttat ccagaagct 
ccgactcca agctatcca ggagctcca acccctggt 
tatccagga gctccgtct ccgggctat ccaggcgca 
ccagccccg ggttaccca gaagcccca tatccgggc 
tacccagga gttccatct cctggctat ccaggggct 
ccatctcct ggttatccg ggcgtacca tcaggtcca 
ggtattcca agttatcca caaggtcca cctccaggt 
gctcctggt tatccagga gttccaaca ggtccaagt 
tatcccgga gcgccagga ggacccggc taccccgaa 
ccaagacca ggtcaacca ggttatcca ggagtacca 
cccggttat cccggagcc ccaggcgaa actccaagt 
aggctcact tatcctgga gctcctggc caacccgga 
tatcccggt gcaccaacg ggagaagaa gccgacggt 
gccgactct caagtacaa acatccgtc caacaaacc 
aataacgaa acacaagca agtgctcaa gctcaagga 
cgatacgct ggaggaatg gcccagtct caagtttcc 
ggtacttac agcggttcc ggttctttc agtgcttca 
gctggaagt gatgatggc aaaaaaggg gcattatca 
caagtttcc gggggtaag gaaggtgca ttgagctca 
gctcaagga agaggcggc ttgggtcaa agtcaagca 
caagttcaa ctagcatca gaaagtggt gataccata 
tcaagtgct cagacggat ggatggaac cacggttcg 
caaacacag gtacaagca agtgggaaa ggaggaatg 
gcagatgct caagcgaac ggacctgga agcacctcg 
tctcaggct caaattggg ttcactccg tatgacgaa 
aacgaaaga aacgaccaa tcgacgcct tttagaggc 
ggaggaaca gcaagtgcc caaagtggt acttactca 
ggacaagcc caaagtcaa atccagggg aagtttcaa 
tacggcatt aaatacacg ggagctgca caagccggt 
tcagggagc agagacaca atagctttg gctcggaac 
cagtcaaga cctgaagga cttacagga accttccca 
aaattcaca cctttgaat ttcacttct gggcgaaac 
ccaaacact aatccacaa gacttactg atgcaaact 
agccgaaaa tcagttcat aaaacacta agtatggac 
gtagcaacc caaaaggca ccaactgaa gaccaaact 
gttccaatc cctgacgaa accgaagac acagctaca 
gaagactac catgacgaa gactacgac tacgactca 
gactactcc acccaaaaa cccataacc aaaagcata 
atctcccaa tcctccaaa acgcaacgc cagcacatc 
gtgctcgac cctctcgaa gacctcgat gccacagtg 
caacaaagc tacgggtac cctcccgat ggtaccatc 
ctccaacct ggagaaacg attcctggc tctcccggc 
taccagatc cctgccggg ttccgggga cgtgtcaag 
gccatcgct aacgggccg aacacctac gccacgggc 
agaaactcg caagctcag agtgtgaca ctgacacca 
gggactgga cgcattatt tacaaaaaa ccgatctat 
gcagtcacc tctaattcg caccaaaac ggacatatg 
ggcttcggg tcgggatat acttatcaa ccggcgacg 
tatcaagtc aagagcggg aaatctcta ccgaatttt 
atttccctg agcaagtcg gaaacaggg tctaagaac 
ccttacact ggacaaaag accccgaag gtgtattat 
gcccagtct tcgagttgt ggggttttc acgaatact 
tgcgttttt aatggaagt ggaaaactg tgctttccc 
aagcccaag actaacccc gatgggagt tttatgacg 
tgttga
    mRNA sequence
    • >TC002804 RA
      tatcttcta ggggacacc atgtcaggg gtcacactt 
gaccaggaa tagattcag catttattg ataggccgc 
cctgcggtc acttactcc catggcgcg gttaatttt 
aatttgtgt catagtgag tgcttatct aggtggcat 
attttcgca ggaaaaaac agaggttgt ttctaatac 
tgaagatcc atctctgtc caactcgat acttaaagg 
agcaacaaa gtcgggaac tttgtttga ttaccaaac 
tagagtagt agcttataa agatctcat agaatcaag 
atgtttgat agaattttt aaaagctag cgaaagcta 
cattggaca gtcgaacca taaaccacg gctggataa 
tgtcaccaa gaatttttt tatgactga aaatcagct 
ctccaaaag acaaatatg gacaatagc tgggcaaag 
agttgtaga tttttgtcg cggttttcc aacttatct 
catccacga tttaaaaac aaacgacca aaacagtat 
ttttcaaga cagtctaaa cgagggtat tgccgcaag 
aaaaaaacg tgcaactaa acatttttt cgttggaag 
ggttagagg acaaacaca atcacaata tctaaatta 
tggtaacag caaagatcc tggaaaagc tgaagccga 
atcaagtta tggaagttc aaaggcagt agtcagtgg 
tagtagcgg aatgggcca agcccagag tcaaagtgt 
tccagtggg ttgtgacga atgttatgg cgaaggtta 
cgatcaaga acaagggct ttattatgg aagacctgg 
gggtacacc ggaatttcc gggagaaca atatcgcaa 
gcctggaag acctagcat tccgggata tccagctga 
acctggagg aagacccgg agttcccgg atatcctag 
agggccaga agaaagacc cggatatcc aggaggacc 
cgcagttcc cggatatcc gggattacc aggaggtag 
acccggaat tcctgaata cccgggagc accaggggg 
acgacctgc aattcctgg atatccagg aggaccaga 
aggaagacc cgaaattcc aggatatcc aggaaaacc 
agaagaaag acctggcgt tccgggatt tccagggag 
accagatgg gcggcctgg tgtgccagg atatccagg 
aagaccagg agaacgacc agaaatacc cggatatcc 
tcatatacc tgagggaag accaagaat tcctggata 
tccaggaag acccgaaga aagacctgc atatccaga 
gggaccagt agaaaaacc tggaattcc tggttatcc 
tgagggacc agaaggtag acccggatt tccaggacg 
accagaaga aagacctgt agtccccgg ctacccgag 
tttaccagg gggaagacc gggagtacc gggttatcc 
aagaggacc ggatggacg gccagcatt tccagggta 
tccacaacc tggagttcc cggatattc aaaacaacc 
cggacaact acctggagt tcccggagt accaggata 
tcccggtga acccgaagc aaaacctgg tgtaccagg 
ataccctcg accgggagt tcctgggta ccctggggg 
agaaatacc gtcgcatcc aggaggaga aaagcccgg 
atatccgct ttcacctac tggacctgg agtaccggg 
ttaccctgg ttatcctgg ctacccaca gtaccctac 
agggccttc tccaggact accagaagg tccttcacg 
gcccggtta tcctagcgg tccggaaaa gccaggata 
tcctccaac tgaaggcta tccattaag accgggata 
tccaacacc acccagcta tccagtgcc gggagaagt 
gggatatcc tactccacc tggaaaagt ttccaccgg 
caaatttag tggacaact ttccggaga ttatgaagc 
agaagttgg acaaagggg taaattttc tggaatctt 
ttccggaca atatgaagc tcatcataa tggaaaaat 
tgccggaaa atatgttga tggaaaata ctatccagg 
ccagcctgc aggtcctag ttacccggg atatcccgg 
cttaccaac tccaccagg ttatcctgg acctgcacc 
ttatcctac tgcacccgg cggcgtagg caaaatcac 
gtatcccgt aggacctgg tggagtacc gggttaccc 
tggaacacc aacttatcc aagcattcc cggatatcc 
aacacccgg acacccagt aacagaacc gggatatcc 
aggagtacc aagttaccc gggagcacc acgtccagg 
agtaccatc cggcccaag cggtccaag ttatcccgg 
tggtcaaat ccccggcta tccaggagt accatcacc 
gggtttccc aggaggtcc acctccagg ttatccagg 
agttccaac ggcaggtta tccaggaat tccaactgg 
cccaggggt tccaagtta ccctggagg tccttctgt 
tagttttcc cggagtacc atcagttcc tccaggagt 
tccaagtta tccaggagc tccatctcc tggctatcc 
aggagcccc atcttctgg ctatccagg agctccatc 
gcctggcta tccaggagc tccatcgcc tggctatcc 
aggagctcc atcgcctgg ctatccagg agccccata 
tcctggtta tccagaagc tccgactcc aagctatcc 
aggagctcc aacccctgg ttatccagg agctccgtc 
tccgggcta tccaggcgc accagcccc gggttaccc 
agaagcccc atatccggg ctacccagg agttccatc 
tcctggcta tccaggggc tccatctcc tggttatcc 
gggcgtacc atcaggtcc aggtattcc aagttatcc 
acaaggtcc acctccagg tgctcctgg ttatccagg 
agttccaac aggtccaag ttatcccgg agcgccagg 
aggacccgg ctaccccga accaagacc aggtcaacc 
aggttatcc aggagtacc acccggtta tcccggagc 
cccaggcga aactccaag taggctcac ttatcctgg 
agctcctgg ccaacccgg atatcccgg tgcaccaac 
gggagaaga agccgacgg tgccgactc tcaagtaca 
aacatccgt ccaacaaac caataacga aacacaagc 
aagtgctca agctcaagg acgatacgc tggaggaat 
ggcccagtc tcaagtttc cggtactta cagcggttc 
cggttcttt cagtgcttc agctggaag tgatgatgg 
caaaaaagg ggcattatc acaagtttc cgggggtaa 
ggaaggtgc attgagctc agctcaagg aagaggcgg 
cttgggtca aagtcaagc acaagttca actagcatc 
agaaagtgg tgataccat atcaagtgc tcagacgga 
tggatggaa ccacggttc gcaaacaca ggtacaagc 
aagtgggaa aggaggaat ggcagatgc tcaagcgaa 
cggacctgg aagcacctc gtctcaggc tcaaattgg 
gttcactcc gtatgacga aaacgaaag aaacgacca 
atcgacgcc ttttagagg cggaggaac agcaagtgc 
ccaaagtgg tacttactc aggacaagc ccaaagtca 
aatccaggg gaagtttca atacggcat taaatacac 
gggagctgc acaagccgg ttcagggag cagagacac 
aatagcttt ggctcggaa ccagtcaag acctgaagg 
acttacagg aaccttccc aaaattcac acctttgaa 
tttcacttc tgggcgaaa cccaaacac taatccaca 
agacttact gatgcaaac tagccgaaa atcagttca 
taaaacact aagtatgga cgtagcaac ccaaaaggc 
accaactga agaccaaac tgttccaat ccctgacga 
aaccgaaga cacagctac agaagacta ccatgacga 
agactacga ctacgactc agactactc cacccaaaa 
acccataac caaaagcat aatctccca atcctccaa 
aacgcaacg ccagcacat cgtgctcga ccctctcga 
agacctcga tgccacagt gcaacaaag ctacgggta 
ccctcccga tggtaccat cctccaacc tggagaaac 
gattcctgg ctctcccgg ctaccagat ccctgccgg 
gttccgggg acgtgtcaa ggccatcgc taacgggcc 
gaacaccta cgccacggg cagaaactc gcaagctca 
gagtgtgac actgacacc agggactgg acgcattat 
ttacaaaaa accgatcta tgcagtcac ctctaattc 
gcaccaaaa cggacatat gggcttcgg gtcgggata 
tacttatca accggcgac gtatcaagt caagagcgg 
gaaatctct accgaattt tatttccct gagcaagtc 
ggaaacagg gtctaagaa cccttacac tggacaaaa 
gaccccgaa ggtgtatta tgcccagtc ttcgagttg 
tggggtttt cacgaatac ttgcgtttt taatggaag 
tggaaaact gtgctttcc caagcccaa gactaaccc 
cgatgggag ttttatgac gtgttgagg gttgtttgt 
attgtaagt taattaagg ttttgtaaa ttatgtcgt 
gtcataagt tttaaataa agtttttga ttattt
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