Status
Please wait ...
Gene information
General information
TC002803 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 9644702..9653759
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002803
    iBeetle screen iB_03453

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially rounded - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: unclear, whether phenotype is a real muscle phenotype or effect is secundary due to the death of the larvae leg muscles not affected
    larval musculature partially shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition >5 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002803 RA
      MPRAFLITH RRYNLDGVE QKELNSENA VSIADEGDS 
      QPSECPDEL YNLTKLAEV AVATGQILE QTRLSSSND 
      QPVFTYTHK LFDKSSRAP RPHLIKTEE YNRKIVSSS 
      TVNIPHSTT QDGVLGESN DHECNDCGK RYSTSSNLA 
      RHRQTHRSP GDKKARRCP HCDKLYVSM PAFSMHVRT 
      HNQGCKCQY CGKCFSRPW LLQGHIRTH TGEKPFKCT 
      ICNKAFADK SNLRAHIQT HSNTKPHIC GRCGKAFAL 
      KSYLYKHEE SSCMRINGR HSSREATPD KVASPTPVI 
      VSGPRISDS VIKSPERPL LYRSTVISP NPERLLCST 
      IKHPSVILN TSARFGIGQ IFQEQPMDF SANRREYGL 
      AIAV
    CDS sequence
    • >TC002803 RA
      atgccgcga gctttcctc atcacacat cggcggtac 
      aacttggat ggagtggag cagaaagaa ctaaactcg 
      gaaaatgct gtcagtatc gccgacgag ggcgactcc 
      cagccctcg gaatgcccc gacgagctg tacaatttg 
      accaaattg gccgaagtg gcggtagcc accgggcaa 
      atcctggaa caaaccagg ctttcctcc tccaacgac 
      caaccggtc ttcacctac acccataag ctgttcgat 
      aagtcctca cgggcaccc aggccacat ctaatcaag 
      actgaagaa tacaataga aaaattgtt tcgtcttct 
      accgtaaat ataccgcat agcaccacc caggatggg 
      gttctgggc gagagtaat gaccacgaa tgcaatgat 
      tgtggcaaa agatacagc acttcgagt aatttagcc 
      agacatagg caaacgcat agaagtccg ggggataag 
      aaggctaga cgttgcccc cactgtgac aaactttat 
      gtcagcatg ccggcgttt tcgatgcat gtgaggact 
      cacaatcaa ggctgcaag tgtcagtat tgcggcaag 
      tgtttctcg aggccttgg ctgcttcaa ggacacatc 
      aggacccac acaggcgaa aagccgttc aaatgcacc 
      atctgcaac aaggccttc gccgacaag tccaacctg 
      cgtgcccac atccagacg cactccaac accaagccc 
      cacatttgc ggccgctgt ggcaaagcc ttcgccctc 
      aagtcctat ctctacaaa cacgaggag tcctcctgt 
      atgcgcatc aacggacgg cactcctcg cgcgaagcc 
      actccggat aaagtcgcc tctccaact ccggtgatc 
      gtttccggg ccccggatc agcgacagc gttatcaaa 
      agtccggag cgaccgctt ttgtaccgc tcgactgtc 
      atttcgccg aatccggag cgccttctc tgctcgacc 
      atcaaacat ccgtcggtt attttgaac acttcggct 
      aggttcggg attgggcag atttttcag gaacagccg 
      atggatttt tcagcgaat cgaagggag tatgggtta 
      gctatcgcg gtttaa
    mRNA sequence
    • >TC002803 RA
      aagctcaaa gcactaaag aagtaagtc actggtggc 
      ctcttcacc gagtgattc ggtgtgcgt gcttcatgt 
      caacacgac tcatttgaa gaaaccgaa tttcctgaa 
      agcatgccg cgagctttc ctcatcaca catcggcgg 
      tacaacttg gatggagtg gagcagaaa gaactaaac 
      tcggaaaat gctgtcagt atcgccgac gagggcgac 
      tcccagccc tcggaatgc cccgacgag ctgtacaat 
      ttgaccaaa ttggccgaa gtggcggta gccaccggg 
      caaatcctg gaacaaacc aggctttcc tcctccaac 
      gaccaaccg gtcttcacc tacacccat aagctgttc 
      gataagtcc tcacgggca cccaggcca catctaatc 
      aagactgaa gaatacaat agaaaaatt gtttcgtct 
      tctaccgta aatataccg catagcacc acccaggat 
      ggggttctg ggcgagagt aatgaccac gaatgcaat 
      gattgtggc aaaagatac agcacttcg agtaattta 
      gccagacat aggcaaacg catagaagt ccgggggat 
      aagaaggct agacgttgc ccccactgt gacaaactt 
      tatgtcagc atgccggcg ttttcgatg catgtgagg 
      actcacaat caaggctgc aagtgtcag tattgcggc 
      aagtgtttc tcgaggcct tggctgctt caaggacac 
      atcaggacc cacacaggc gaaaagccg ttcaaatgc 
      accatctgc aacaaggcc ttcgccgac aagtccaac 
      ctgcgtgcc cacatccag acgcactcc aacaccaag 
      ccccacatt tgcggccgc tgtggcaaa gccttcgcc 
      ctcaagtcc tatctctac aaacacgag gagtcctcc 
      tgtatgcgc atcaacgga cggcactcc tcgcgcgaa 
      gccactccg gataaagtc gcctctcca actccggtg 
      atcgtttcc gggccccgg atcagcgac agcgttatc 
      aaaagtccg gagcgaccg cttttgtac cgctcgact 
      gtcatttcg ccgaatccg gagcgcctt ctctgctcg 
      accatcaaa catccgtcg gttattttg aacacttcg 
      gctaggttc gggattggg cagattttt caggaacag 
      ccgatggat ttttcagcg aatcgaagg gagtatggg 
      ttagctatc gcggtttaa aagtcgatt taaattccg 
      tcaagggac cattgtgaa ttcaagtgc cattgtatg 
      tttgccctt gggcagctt ctgattgta gtaattttg 
      taaataagt ggacgatac tattagccg ataattttt 
      cttttaagt tcaaagtgc ctcaagttg tactagacg 
      agagtgtct ctagtcttg gagcgcatt tcgtcgaac 
      gaagttcga ccgaaatgc gtagtcatt ttaaatttt 
      aagttattt atttctact aacaagtaa taatagttt 
      tagttggtt tttaacgta tcggctaat gatatagct 
      tttcctagt caaggcaag ttggtcgca acttgtaag 
      gccacaagt gcaatatac ttaagaatt ttatttaat 
      cgtaattat ttattcgtg tggtaaatt atcgtagat 
      agtttcttg tttattgcc aaaaaattc attcctaga 
      ggatttaga gtttgtcat tcccgtata acagtctac 
      ctttttggt gcaaccgtt tgtgaaatt cgttttctt 
      acttttgtg tacttttat accgaacat tttttaata 
      aacgtgcaa tctaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY