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Gene information
General information
TC002740 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 12809543..12816115
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC002740
      iBeetle screen iB_03447

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC002740 RA
        MDSLSLELL EFILSSPVL SDRDVLNFS STCHHFRNI 
        VLTSEVIWR RRFYRKLPS FSDNSWCPD SYYEELKYF 
        YSLKPRVLD MLESAFVDF FSESICPKV NLSDWEMLY 
        NEKAPNYFY LRRHLLDIN NTKEAINSI LIVPFNTPG 
        HLTLQFFAR FSLSFLNEI YLKNTWPKF IRAPPKQQI 
        FEKLAILVA QWFHLDTET VELYVTKGL DNFANLVKM 
        RLKATHPKH PIFATQEIK WKKEKLDKH QWEPEDCQV 
        IISTIRDVL YSDLGFQEC DHEWEFITR ILESRKCLP 
        TNLAVIYES VARRLGVLV DVVCDFSRH KYCFLQLQL 
        NETIYYINP GNGCFMRGV TSSKRVTSG VIVQQMIDN 
        LQHKFLDVN DRDKNELIR SLCRLNDII NNVYISSPL 
        RLLYHQIRK CGNNNKRPI ALKYAVGMV VVLNNAISW 
        STGTPISGK GVIFDWNRS EYEEDYLYG VICDNGEAL 
        SLMDYKDAL TPTADPGNL QSIEQVALY FSHFFVNHF 
        VPNLEVEKL FPYDGEVRQ KFYHQLQAR DGRV
      CDS sequence
      • >TC002740 RA
        atggattct ttgtcactt gagttgctt gagtttatt 
        ctaagtagc cctgttcta agtgacagg gacgtactt 
        aatttttcc tcaacatgt caccatttc cgtaatatt 
        gttctaacc agtgaagtg atatggaga cgaagattt 
        tacaggaaa ttgccaagc ttttccgat aattcctgg 
        tgtcctgac tcgtattat gaagaactt aaatatttt 
        tactcattg aaacctagg gtgcttgat atgttagaa 
        tcggccttt gttgatttt ttctcagag agcatttgt 
        cctaaggtg aacttaagc gactgggaa atgctttat 
        aatgaaaag gcaccaaat tatttttac ttacgtaga 
        catctcctc gacattaat aatacaaaa gaagcgata 
        aatagtatc ctaattgtg cctttcaat actccaggt 
        catttaacg ttgcaattt ttcgcacga ttcagtttg 
        agcttcttg aatgaaata tatctgaaa aatacttgg 
        cccaaattc atccgtgct ccacctaag caacaaatt 
        tttgagaaa cttgctatt cttgtcgca cagtggttt 
        caccttgac actgaaaca gtcgagctt tatgttaca 
        aaaggactt gacaatttt gcaaatttg gtaaaaatg 
        agactgaag gctacgcac cccaaacat ccaattttc 
        gccacccaa gaaatcaag tggaaaaaa gaaaagttg 
        gacaaacac caatgggag cctgaagat tgccaagtg 
        atcatttcc acaatccgt gatgttctc tactcggat 
        ctggggttc caagaatgt gatcatgag tgggagttt 
        attactaga attttagaa agtcgcaaa tgcctcccg 
        acgaatttg gcggtgatt tacgaaagt gtggctagg 
        cgtttgggg gtcctggtc gatgtggtc tgcgatttc 
        agtcgtcac aagtattgt tttcttcaa cttcaatta 
        aatgaaacg atttattac attaatcct ggcaatggg 
        tgttttatg cgcggagtg acttcttca aagagagtt 
        accagcggc gtaatagtt caacaaatg attgacaat 
        ttgcaacat aaattcctg gatgtaaat gacagagac 
        aaaaacgaa ctgattcgg tcgttgtgt cgccttaat 
        gatatcatt aacaacgtc tatatctcg tcaccactg 
        agactactc taccatcaa attcgcaaa tgtggaaat 
        aacaataaa cggcccatt gctctaaag tatgccgtg 
        ggtatggta gtggttctt aacaatgct ataagctgg 
        agtacaggc acgcctatt agtgggaaa ggtgtgatt 
        tttgattgg aatagatcg gagtatgag gaggattat 
        ctgtatggg gtcatttgt gataatgga gaagcacta 
        agccttatg gattataaa gatgcatta actccgacc 
        gctgatccg ggtaactta caatcgata gaacaagtt 
        gctctatac ttttcacac tttttcgtt aaccacttc 
        gttccaaat ttagaagtg gagaaactt tttccttat 
        gatggggaa gtgagacaa aagttctac caccaactg 
        caggcccgt gatggaaga gtttag
      mRNA sequence
      • >TC002740 RA
        aatctgtcg tttggcaaa atggattct ttgtcactt 
        gagttgctt gagtttatt ctaagtagc cctgttcta 
        agtgacagg gacgtactt aatttttcc tcaacatgt 
        caccatttc cgtaatatt gttctaacc agtgaagtg 
        atatggaga cgaagattt tacaggaaa ttgccaagc 
        ttttccgat aattcctgg tgtcctgac tcgtattat 
        gaagaactt aaatatttt tactcattg aaacctagg 
        gtgcttgat atgttagaa tcggccttt gttgatttt 
        ttctcagag agcatttgt cctaaggtg aacttaagc 
        gactgggaa atgctttat aatgaaaag gcaccaaat 
        tatttttac ttacgtaga catctcctc gacattaat 
        aatacaaaa gaagcgata aatagtatc ctaattgtg 
        cctttcaat actccaggt catttaacg ttgcaattt 
        ttcgcacga ttcagtttg agcttcttg aatgaaata 
        tatctgaaa aatacttgg cccaaattc atccgtgct 
        ccacctaag caacaaatt tttgagaaa cttgctatt 
        cttgtcgca cagtggttt caccttgac actgaaaca 
        gtcgagctt tatgttaca aaaggactt gacaatttt 
        gcaaatttg gtaaaaatg agactgaag gctacgcac 
        cccaaacat ccaattttc gccacccaa gaaatcaag 
        tggaaaaaa gaaaagttg gacaaacac caatgggag 
        cctgaagat tgccaagtg atcatttcc acaatccgt 
        gatgttctc tactcggat ctggggttc caagaatgt 
        gatcatgag tgggagttt attactaga attttagaa 
        agtcgcaaa tgcctcccg acgaatttg gcggtgatt 
        tacgaaagt gtggctagg cgtttgggg gtcctggtc 
        gatgtggtc tgcgatttc agtcgtcac aagtattgt 
        tttcttcaa cttcaatta aatgaaacg atttattac 
        attaatcct ggcaatggg tgttttatg cgcggagtg 
        acttcttca aagagagtt accagcggc gtaatagtt 
        caacaaatg attgacaat ttgcaacat aaattcctg 
        gatgtaaat gacagagac aaaaacgaa ctgattcgg 
        tcgttgtgt cgccttaat gatatcatt aacaacgtc 
        tatatctcg tcaccactg agactactc taccatcaa 
        attcgcaaa tgtggaaat aacaataaa cggcccatt 
        gctctaaag tatgccgtg ggtatggta gtggttctt 
        aacaatgct ataagctgg agtacaggc acgcctatt 
        agtgggaaa ggtgtgatt tttgattgg aatagatcg 
        gagtatgag gaggattat ctgtatggg gtcatttgt 
        gataatgga gaagcacta agccttatg gattataaa 
        gatgcatta actccgacc gctgatccg ggtaactta 
        caatcgata gaacaagtt gctctatac ttttcacac 
        tttttcgtt aaccacttc gttccaaat ttagaagtg 
        gagaaactt tttccttat gatggggaa gtgagacaa 
        aagttctac caccaactg caggcccgt gatggaaga 
        gtttagaag aaatattat ttttccata caaatttag 
        tttatgcgc ttgtttttg aaatatgta cttttttac 
        tatcaaagt tatgtgtta gataggaat aaaatttaa 
        gttcttgt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY