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Gene information
General information
TC002734 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 12860975..12868564
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002734
    iBeetle screen iB_03445

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002734 RA
      MQHQYWVTK KSVLRKLGG KEDECIVSS DAELDAKLE 
      LFKSINDSC VQLQRVIDL YQERLCYLA QEENALGRY 
      LKEYGKNEK NASAGQIMS TAGKALAYT GHQRLTVRP 
      PLVRLHHEV ETFRGRAVS DTRATVIEM EKARTEYRA 
      ALSWMKSVS TQLDPDTGH GLDRFRKAQ SYVKSTKTK 
      FDRLTLACL QKVDLLAAA RCNMFSHAL VPYQGALAT 
      FAAKATETL TLAANKVGD VQPYDFSAI SELATPQHE 
      KDKDTFFNA EYTDKESEK KDEKGESQD LQVSNLLGE 
      EFSSPAPEP EPPVQDGSN PSSALLDLC WPSNSDFLG 
      GDFMPSKLI QEGNFDFSQ LEMGKTDDN TKENDKPMK 
      GPGKGNEKN GTQMSWLSL FAELDPLAN ADQELTNSG 
      DRA
    CDS sequence
    • >TC002734 RA
      atgcaacat cagtattgg gtgacgaaa aaaagtgta 
      ttgcgtaaa ttaggcggc aaggaagac gaatgtatc 
      gtgtcatca gacgccgag ctcgacgcc aaactcgaa 
      ctcttcaaa tccataaac gatagctgc gtccagtta 
      caacgagtc atagacctg taccaggag cgactttgt 
      tacctggcc caggaggaa aacgccctc ggccgatac 
      ttgaaagaa tacggcaag aatgagaaa aacgccagc 
      gccggacaa atcatgtcg acagctggc aaagcgctg 
      gcttacacg gggcaccag aggctcacc gtgcgcccc 
      cctctggtg cgcctgcac cacgaagtg gagacgttc 
      cgcggacgc gccgtttcc gacacccgt gccactgta 
      atcgaaatg gaaaaggcc cggacggag tatcgcgcc 
      gcgttaagt tggatgaaa tcagtctcc acacagttg 
      gatccagac acggggcac ggcctcgac cggttccgc 
      aaggcgcag tcctatgta aaaagcacc aaaacgaag 
      tttgaccga ctgacgctg gcgtgtctc caaaaggtg 
      gatttgctc gcagcggcg cgatgtaat atgttctcc 
      catgcactc gtgccctac cagggggct ctggccacg 
      ttcgcggcc aaagcaacc gagacgctc acactggcg 
      gccaataaa gtaggagac gttcaaccg tacgatttt 
      agcgcaatc tcggaactg gcaacgccg cagcacgaa 
      aaggacaaa gacacgttt ttcaacgcg gagtacacc 
      gacaaagag tcggagaag aaagacgag aaaggagaa 
      tctcaagac ttgcaagtg tcgaatttg ctaggagaa 
      gagttctca tcaccggct cccgaaccg gagcctccc 
      gtccaagat ggctcaaat ccgagctcg gccttgctt 
      gatttgtgt tggccgagt aatagtgat tttcttggg 
      ggcgacttc atgccctcg aagctcata caggagggg 
      aattttgat tttagccaa ttggaaatg gggaagacg 
      gacgacaat accaaagaa aacgataaa ccaatgaaa 
      ggccccggg aaagggaat gagaaaaac ggaactcaa 
      atgtcatgg ctgagtttg tttgcagaa ttagatcca 
      ttggccaac gccgaccag gagctgacc aattcgggc 
      gatagggcg taa
    mRNA sequence
    • >TC002734 RA
      cataaatgt caaatgtca tgtcaatca ttttgattt 
      ttgcgtgta gacacgcat taatgtttt gaataaaat 
      taaaatagt gtttaaacg atgcaacat cagtattgg 
      gtgacgaaa aaaagtgta ttgcgtaaa ttaggcggc 
      aaggaagac gaatgtatc gtgtcatca gacgccgag 
      ctcgacgcc aaactcgaa ctcttcaaa tccataaac 
      gatagctgc gtccagtta caacgagtc atagacctg 
      taccaggag cgactttgt tacctggcc caggaggaa 
      aacgccctc ggccgatac ttgaaagaa tacggcaag 
      aatgagaaa aacgccagc gccggacaa atcatgtcg 
      acagctggc aaagcgctg gcttacacg gggcaccag 
      aggctcacc gtgcgcccc cctctggtg cgcctgcac 
      cacgaagtg gagacgttc cgcggacgc gccgtttcc 
      gacacccgt gccactgta atcgaaatg gaaaaggcc 
      cggacggag tatcgcgcc gcgttaagt tggatgaaa 
      tcagtctcc acacagttg gatccagac acggggcac 
      ggcctcgac cggttccgc aaggcgcag tcctatgta 
      aaaagcacc aaaacgaag tttgaccga ctgacgctg 
      gcgtgtctc caaaaggtg gatttgctc gcagcggcg 
      cgatgtaat atgttctcc catgcactc gtgccctac 
      cagggggct ctggccacg ttcgcggcc aaagcaacc 
      gagacgctc acactggcg gccaataaa gtaggagac 
      gttcaaccg tacgatttt agcgcaatc tcggaactg 
      gcaacgccg cagcacgaa aaggacaaa gacacgttt 
      ttcaacgcg gagtacacc gacaaagag tcggagaag 
      aaagacgag aaaggagaa tctcaagac ttgcaagtg 
      tcgaatttg ctaggagaa gagttctca tcaccggct 
      cccgaaccg gagcctccc gtccaagat ggctcaaat 
      ccgagctcg gccttgctt gatttgtgt tggccgagt 
      aatagtgat tttcttggg ggcgacttc atgccctcg 
      aagctcata caggagggg aattttgat tttagccaa 
      ttggaaatg gggaagacg gacgacaat accaaagaa 
      aacgataaa ccaatgaaa ggccccggg aaagggaat 
      gagaaaaac ggaactcaa atgtcatgg ctgagtttg 
      tttgcagaa ttagatcca ttggccaac gccgaccag 
      gagctgacc aattcgggc gatagggcg taatgatct 
      gaactacga tatgtttta tttttaagt attatacac 
      agtcttgca taatgtata taacattcc tttgtgatt 
      cttttgcac tagtcataa ccaaattgc attgtttaa 
      acctatatg aataaaatg ttcacgaaa gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY