Status
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Gene information
General information
TC002731 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 12897015..12905154
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002731
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03444

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    previtellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 3

    head orientation irregular - Number of animals showing the phenotype: 3
    wings shortened - Number of animals showing the phenotype: 3

    oblique dorsal thoracic muscles shortened - Number of animals showing the phenotype: 3

    longitudinal dorsal thoracic muscles fluorescence decreased - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002731 RA
      MYTRIEYLN ICVLLIIGF ANCVSSYHV DIIDNGPIV 
KGANITFKA TVYYGDKFA SGTFMYSWK DRAVPTHTR 
EFQSKNPID EWTVLYNAT IYPQGPYVV QVTVCKYFL 
VYCAEIGSN RVEFYINET LNGDILLIQ KNKTRPDLF 
VSNAEPVIH NVSLKQTDA DYIKQAPSV LTYWFVDCV 
YYGFRKDFE FPFNYTQAE KEHLIEALV VADFSPPPP 
PPTTTVPTT TTTTTTIKP NTTTTSTTT PKPTTTTKP 
TNSVTTPTT TTATPTNST KVKRDVDSQ SKIMVNVNG 
TLQPFKGNF PFVCNGTQV ATDSHKTYG YFFKRVKVR 
APVAKVNVT GNNWLQHGD LLTLNVKCH GSRNFKYCV 
QYQRGLYNV TGNETCYIY QELDKCEFT IQRYFSDSM 
EHTVVIIIE NEVGKVVYP VTVTVYKVK KQAQLSVIV 
VPVAFSLVA VVLIVFGVA YYLQNRSRF VVEVADFNF 
GQNYSDMEY KTFKERLRD AIANAFFTR APSPGSSEV 
PVWPPGRKY GSMTS
    CDS sequence
    • >TC002731 RA
      atgtacact cgaatagaa tatctaaat atttgtgtt 
ttattaata ataggtttt gcaaattgt gtctcatct 
tatcatgta gacattatc gacaatgga cctatcgtg 
aaaggagcc aatataaca tttaaagcg accgtttat 
tacggtgat aagtttgct agcggaact tttatgtat 
agctggaag gacagagct gtcccaaca cacacaaga 
gagttccaa tctaaaaat ccaattgat gaatggact 
gttttgtac aatgccact atttatcct caaggtcct 
tatgtggtt caagtcaca gtctgtaaa tattttttg 
gtttattgc gctgaaatc ggctcaaat agggtagag 
ttctatata aatgaaacc ttaaacggt gacatttta 
cttattcaa aaaaacaaa acacgaccg gatctcttc 
gtctcaaac gcagaacct gtcatccac aacgtgtct 
ctcaaacaa accgacgcc gactacatc aagcaagct 
cccagtgtg ctaacgtat tggtttgtc gattgcgtc 
tattatggg tttaggaaa gactttgaa tttccgttc 
aattacacg caagctgag aaagaacac ttgattgaa 
gctttggta gttgctgat ttttcgcca ccacctcca 
cctcccacc acaaccgtt ccaaccacc accaccaca 
acgacaaca atcaagcct aacactacc accaccagc 
acaacaact cctaaacca acaaccaca actaaacca 
accaacagt gtaacaaca ccaacaact acaacagca 
acaccaacg aattcaacc aaagtcaaa agagatgta 
gatagccaa tcaaaaatt atggtgaat gtgaatgga 
acgttacag ccttttaag ggcaatttt ccctttgtt 
tgtaatgga actcaagtc gccacagat tcacataaa 
acttacggc tacttcttc aagagagta aaagttaga 
gcgcctgta gccaaagtg aacgtcact ggaaataat 
tggctacaa catggggat ttgttaacg ctaaatgtg 
aaatgtcac ggatcgaga aatttcaaa tattgcgtg 
caataccaa agagggctt tataatgta acagggaac 
gaaacttgt tacatttac caagaatta gacaagtgt 
gaattcaca atccagagg tacttttcc gattctatg 
gaacataca gtggttatt ataatcgaa aatgaagtc 
ggtaaagtc gtctatcct gttacagta actgtttat 
aaagttaaa aaacaagcg caactttcc gtaattgtg 
gtacctgtg gctttcagt ttagttgct gtggtttta 
attgtgttt ggagtggcg tattatctc caaaaccgt 
tcaaggttt gtagtggag gtggcagat tttaatttc 
ggtcagaac tacagtgat atggagtat aagactttt 
aaggagaga ttgagagat gcgatcgct aatgcgttt 
tttacaagg gctcctagt cctggatcg tcggaagtt 
ccggtgtgg ccaccaggc cggaaatac ggaagcatg 
acgagttaa
    mRNA sequence
    • >TC002731 RA
      aatggcgac actgtgcca atgtttttg gtgttttga 
cattgacgt ttctacgtt tttgtgaat ttttatgtt 
ttgaatggc tttctttcg ttgaaccta attatgtac 
actcgaata gaatatcta aatatttgt gttttatta 
ataataggt tttgcaaat tgtgtctca tcttatcat 
gtagacatt atcgacaat ggacctatc gtgaaagga 
gccaatata acatttaaa gcgaccgtt tattacggt 
gataagttt gctagcgga acttttatg tatagctgg 
aaggacaga gctgtccca acacacaca agagagttc 
caatctaaa aatccaatt gatgaatgg actgttttg 
tacaatgcc actatttat cctcaaggt ccttatgtg 
gttcaagtc acagtctgt aaatatttt ttggtttat 
tgcgctgaa atcggctca aatagggta gagttctat 
ataaatgaa accttaaac ggtgacatt ttacttatt 
caaaaaaac aaaacacga ccggatctc ttcgtctca 
aacgcagaa cctgtcatc cacaacgtg tctctcaaa 
caaaccgac gccgactac atcaagcaa gctcccagt 
gtgctaacg tattggttt gtcgattgc gtctattat 
gggtttagg aaagacttt gaatttccg ttcaattac 
acgcaagct gagaaagaa cacttgatt gaagctttg 
gtagttgct gatttttcg ccaccacct ccacctccc 
accacaacc gttccaacc accaccacc acaacgaca 
acaatcaag cctaacact accaccacc agcacaaca 
actcctaaa ccaacaacc acaactaaa ccaaccaac 
agtgtaaca acaccaaca actacaaca gcaacacca 
acgaattca accaaagtc aaaagagat gtagatagc 
caatcaaaa attatggtg aatgtgaat ggaacgtta 
cagcctttt aagggcaat tttcccttt gtttgtaat 
ggaactcaa gtcgccaca gattcacat aaaacttac 
ggctacttc ttcaagaga gtaaaagtt agagcgcct 
gtagccaaa gtgaacgtc actggaaat aattggcta 
caacatggg gatttgtta acgctaaat gtgaaatgt 
cacggatcg agaaatttc aaatattgc gtgcaatac 
caaagaggg ctttataat gtaacaggg aacgaaact 
tgttacatt taccaagaa ttagacaag tgtgaattc 
acaatccag aggtacttt tccgattct atggaacat 
acagtggtt attataatc gaaaatgaa gtcggtaaa 
gtcgtctat cctgttaca gtaactgtt tataaagtt 
aaaaaacaa gcgcaactt tccgtaatt gtggtacct 
gtggctttc agtttagtt gctgtggtt ttaattgtg 
tttggagtg gcgtattat ctccaaaac cgttcaagg 
tttgtagtg gaggtggca gattttaat ttcggtcag 
aactacagt gatatggag tataagact tttaaggag 
agattgaga gatgcgatc gctaatgcg ttttttaca 
agggctcct agtcctgga tcgtcggaa gttccggtg 
tggccacca ggccggaaa tacggaagc atgacgagt 
taagatatt atctaaaat aatgaaaga ctagtgaga 
ttcgcctaa gattggaat atacgctac agtatagca 
ttgtaggat tttttgtca attttatga caaatgtgt 
ttatgtgat ctgtattta ttacgttat ctcggtttg 
attttttaa atatatttt tggatgcac atgttgttt 
aatacttaa caatgtttt tattcgagg tgatgcagg 
ttttattct gctttaaca ctggagagg gcgagcgta 
ggtcgagtg ctgtataac tgtctgata tattttact 
tttttgttg tattgcttt gtattttct actacatga 
tatattaag gagaaatga agcaaaata aattagttt 
taaaatg
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