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Gene information
General information
TC002731 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 12897015..12905154
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002731
    iBeetle screen iB_03444

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 3

    head orientation irregular - Number of animals showing the phenotype: 3
    wings shortened - Number of animals showing the phenotype: 3

    oblique dorsal thoracic muscles shortened - Number of animals showing the phenotype: 3

    longitudinal dorsal thoracic muscles fluorescence decreased - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002731 RA
      MYTRIEYLN ICVLLIIGF ANCVSSYHV DIIDNGPIV 
      KGANITFKA TVYYGDKFA SGTFMYSWK DRAVPTHTR 
      EFQSKNPID EWTVLYNAT IYPQGPYVV QVTVCKYFL 
      VYCAEIGSN RVEFYINET LNGDILLIQ KNKTRPDLF 
      VSNAEPVIH NVSLKQTDA DYIKQAPSV LTYWFVDCV 
      YYGFRKDFE FPFNYTQAE KEHLIEALV VADFSPPPP 
      PPTTTVPTT TTTTTTIKP NTTTTSTTT PKPTTTTKP 
      TNSVTTPTT TTATPTNST KVKRDVDSQ SKIMVNVNG 
      TLQPFKGNF PFVCNGTQV ATDSHKTYG YFFKRVKVR 
      APVAKVNVT GNNWLQHGD LLTLNVKCH GSRNFKYCV 
      QYQRGLYNV TGNETCYIY QELDKCEFT IQRYFSDSM 
      EHTVVIIIE NEVGKVVYP VTVTVYKVK KQAQLSVIV 
      VPVAFSLVA VVLIVFGVA YYLQNRSRF VVEVADFNF 
      GQNYSDMEY KTFKERLRD AIANAFFTR APSPGSSEV 
      PVWPPGRKY GSMTS
    CDS sequence
    • >TC002731 RA
      atgtacact cgaatagaa tatctaaat atttgtgtt 
      ttattaata ataggtttt gcaaattgt gtctcatct 
      tatcatgta gacattatc gacaatgga cctatcgtg 
      aaaggagcc aatataaca tttaaagcg accgtttat 
      tacggtgat aagtttgct agcggaact tttatgtat 
      agctggaag gacagagct gtcccaaca cacacaaga 
      gagttccaa tctaaaaat ccaattgat gaatggact 
      gttttgtac aatgccact atttatcct caaggtcct 
      tatgtggtt caagtcaca gtctgtaaa tattttttg 
      gtttattgc gctgaaatc ggctcaaat agggtagag 
      ttctatata aatgaaacc ttaaacggt gacatttta 
      cttattcaa aaaaacaaa acacgaccg gatctcttc 
      gtctcaaac gcagaacct gtcatccac aacgtgtct 
      ctcaaacaa accgacgcc gactacatc aagcaagct 
      cccagtgtg ctaacgtat tggtttgtc gattgcgtc 
      tattatggg tttaggaaa gactttgaa tttccgttc 
      aattacacg caagctgag aaagaacac ttgattgaa 
      gctttggta gttgctgat ttttcgcca ccacctcca 
      cctcccacc acaaccgtt ccaaccacc accaccaca 
      acgacaaca atcaagcct aacactacc accaccagc 
      acaacaact cctaaacca acaaccaca actaaacca 
      accaacagt gtaacaaca ccaacaact acaacagca 
      acaccaacg aattcaacc aaagtcaaa agagatgta 
      gatagccaa tcaaaaatt atggtgaat gtgaatgga 
      acgttacag ccttttaag ggcaatttt ccctttgtt 
      tgtaatgga actcaagtc gccacagat tcacataaa 
      acttacggc tacttcttc aagagagta aaagttaga 
      gcgcctgta gccaaagtg aacgtcact ggaaataat 
      tggctacaa catggggat ttgttaacg ctaaatgtg 
      aaatgtcac ggatcgaga aatttcaaa tattgcgtg 
      caataccaa agagggctt tataatgta acagggaac 
      gaaacttgt tacatttac caagaatta gacaagtgt 
      gaattcaca atccagagg tacttttcc gattctatg 
      gaacataca gtggttatt ataatcgaa aatgaagtc 
      ggtaaagtc gtctatcct gttacagta actgtttat 
      aaagttaaa aaacaagcg caactttcc gtaattgtg 
      gtacctgtg gctttcagt ttagttgct gtggtttta 
      attgtgttt ggagtggcg tattatctc caaaaccgt 
      tcaaggttt gtagtggag gtggcagat tttaatttc 
      ggtcagaac tacagtgat atggagtat aagactttt 
      aaggagaga ttgagagat gcgatcgct aatgcgttt 
      tttacaagg gctcctagt cctggatcg tcggaagtt 
      ccggtgtgg ccaccaggc cggaaatac ggaagcatg 
      acgagttaa
    mRNA sequence
    • >TC002731 RA
      aatggcgac actgtgcca atgtttttg gtgttttga 
      cattgacgt ttctacgtt tttgtgaat ttttatgtt 
      ttgaatggc tttctttcg ttgaaccta attatgtac 
      actcgaata gaatatcta aatatttgt gttttatta 
      ataataggt tttgcaaat tgtgtctca tcttatcat 
      gtagacatt atcgacaat ggacctatc gtgaaagga 
      gccaatata acatttaaa gcgaccgtt tattacggt 
      gataagttt gctagcgga acttttatg tatagctgg 
      aaggacaga gctgtccca acacacaca agagagttc 
      caatctaaa aatccaatt gatgaatgg actgttttg 
      tacaatgcc actatttat cctcaaggt ccttatgtg 
      gttcaagtc acagtctgt aaatatttt ttggtttat 
      tgcgctgaa atcggctca aatagggta gagttctat 
      ataaatgaa accttaaac ggtgacatt ttacttatt 
      caaaaaaac aaaacacga ccggatctc ttcgtctca 
      aacgcagaa cctgtcatc cacaacgtg tctctcaaa 
      caaaccgac gccgactac atcaagcaa gctcccagt 
      gtgctaacg tattggttt gtcgattgc gtctattat 
      gggtttagg aaagacttt gaatttccg ttcaattac 
      acgcaagct gagaaagaa cacttgatt gaagctttg 
      gtagttgct gatttttcg ccaccacct ccacctccc 
      accacaacc gttccaacc accaccacc acaacgaca 
      acaatcaag cctaacact accaccacc agcacaaca 
      actcctaaa ccaacaacc acaactaaa ccaaccaac 
      agtgtaaca acaccaaca actacaaca gcaacacca 
      acgaattca accaaagtc aaaagagat gtagatagc 
      caatcaaaa attatggtg aatgtgaat ggaacgtta 
      cagcctttt aagggcaat tttcccttt gtttgtaat 
      ggaactcaa gtcgccaca gattcacat aaaacttac 
      ggctacttc ttcaagaga gtaaaagtt agagcgcct 
      gtagccaaa gtgaacgtc actggaaat aattggcta 
      caacatggg gatttgtta acgctaaat gtgaaatgt 
      cacggatcg agaaatttc aaatattgc gtgcaatac 
      caaagaggg ctttataat gtaacaggg aacgaaact 
      tgttacatt taccaagaa ttagacaag tgtgaattc 
      acaatccag aggtacttt tccgattct atggaacat 
      acagtggtt attataatc gaaaatgaa gtcggtaaa 
      gtcgtctat cctgttaca gtaactgtt tataaagtt 
      aaaaaacaa gcgcaactt tccgtaatt gtggtacct 
      gtggctttc agtttagtt gctgtggtt ttaattgtg 
      tttggagtg gcgtattat ctccaaaac cgttcaagg 
      tttgtagtg gaggtggca gattttaat ttcggtcag 
      aactacagt gatatggag tataagact tttaaggag 
      agattgaga gatgcgatc gctaatgcg ttttttaca 
      agggctcct agtcctgga tcgtcggaa gttccggtg 
      tggccacca ggccggaaa tacggaagc atgacgagt 
      taagatatt atctaaaat aatgaaaga ctagtgaga 
      ttcgcctaa gattggaat atacgctac agtatagca 
      ttgtaggat tttttgtca attttatga caaatgtgt 
      ttatgtgat ctgtattta ttacgttat ctcggtttg 
      attttttaa atatatttt tggatgcac atgttgttt 
      aatacttaa caatgtttt tattcgagg tgatgcagg 
      ttttattct gctttaaca ctggagagg gcgagcgta 
      ggtcgagtg ctgtataac tgtctgata tattttact 
      tttttgttg tattgcttt gtattttct actacatga 
      tatattaag gagaaatga agcaaaata aattagttt 
      taaaatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY