Status
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Gene information
General information
TC002730 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 12946501..12995885
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002730
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03443

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: remnants inside-outed
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    gut remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Adult morphology
    wings not closed - Number of animals showing the phenotype: 6

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002730 RA
      MATICFRPP PLPPNKKSQ LNINRYRKQ SLLSSTYLS 
      FRDPAEDEV IQDEIELTN FKCKKKCKK VAHTNSLSR 
      AKDAKCAMV RSHSDGNLN KEIAPMTIN RYIKVLSGS 
      WKNLLNLGG MSRPPKPAA TAKKVAPPA VPHEFRHSG 
      SSFGSAGYA SSEDAGFLA PNDPPGVPR DDHSDYGST 
      VSSSTGKSP GPIFPAPSF TFPGQPPPG GVLTHKAAV 
      YYHHQLSLQ EDQGIDMTQ SPGRDSPGS SSGSAGSGS 
      RHSTASLDS GRASYHPLS TAGRSTIGS SNSPRCSLS 
      SCSIGSEQG RIERMIHQG VPDQEIIHA WLVDLQYDE 
      YFPLFVSAG YDLPTIGRM TPEDLTAIG IKKPNHRKR 
      LKAEIAQLN LPDNLPEFI PGSLEEWLQ LLRLEEYLP 
      AFLDQGYQT VDDVTQLTW EDLEEFGIV KLGHQKKIM 
      LAIKRVKDI RAGKRINTD SNRIYATQD VMVHAPLGP 
      PSPGVPAVH STFRSFHQP WEIDQSRQL AAGTHYVQP 
      IYCTDIVPI KIRTGRGKS LESLEDPNE RTHHTFSAE 
      NTQTFYYQQ PIGWRTRSY DDGDITPTN ETGLLYEGG 
      GTLPRPRGV IRPRPIAKI AAKARESFP DFEKPQFNP 
      EKYSNPQYK TLPRELIDT TKYHIQYSP MMGKKVPPN 
      PPKRQDSEG AETTTTVEI HHVQTSSPL PLPAYPSSD 
      SLSISLDNQ GDLPLPPPP APGTPPGNV QSKMNSSQS 
      WGAEEQELI KTLALQHRN GSDASFKSS SSTESDSLP 
      FANENAGTI RTRAGGNSR PADYSPAKP RAGLPPHPS 
      PTPNRQPSA RNQRSEPAD VLNDIGNML ANLTDELDA 
      MLEEEKRQQ
    CDS sequence
    • >TC002730 RA
      atggcaacg atctgtttc cgacctcca cctttgccc 
      ccgaacaag aaatcgcaa ttgaacatc aatcgttat 
      cggaagcaa agtttgctc tcgtcgacg tatctgagt 
      ttccgcgat ccggccgaa gacgaggtc atacaagac 
      gaaatcgaa ctgacgaac tttaagtgt aaaaaaaag 
      tgcaaaaaa gtggcgcac acgaactcg ctgagtcgg 
      gccaaggat gcaaaatgt gccatggtg cggagccac 
      tccgacggg aacctcaac aaggagata gcgcccatg 
      accatcaat aggtacatc aaggtgttg agcggcagc 
      tggaagaat ttgctcaac ttaggcggc atgtcccgg 
      cccccgaag ccggccgcc accgccaag aaggtggct 
      ccaccggcg gtgccccac gagttccgg cactcgggc 
      agctccttc gggtccgca ggatatgcc tcttcggag 
      gacgcgggc ttcctggcg ccgaatgat cctcccgga 
      gtcccacga gacgatcat tcggactat ggcagcact 
      gtcagcagc agcacgggc aagtccccc ggacccatt 
      tttccggcg ccctctttc acgtttccg gggcagccg 
      ccccccggc ggcgtcctc acgcacaag gcggccgtc 
      tactaccac caccaactc agcctacag gaggatcag 
      ggtatcgac atgacacag tctccgggc agggacagt 
      ccggggtcg tcgagcgga agtgcagga agtggctct 
      cgccacagc acggcgtct ttagacagt ggcagggcg 
      tcgtaccat cccttgagc acggcagga aggagtacc 
      atcgggtcc tcgaatagt ccacgatgt tccttaagt 
      tcttgctcg ataggatcg gagcaaggc cgaattgag 
      cgaatgatc catcaagga gttcctgac caagaaatt 
      attcatgcg tggctggtt gatcttcag tacgatgaa 
      tatttcccg ttgtttgta agtgccggc tacgatttg 
      ccgacaata ggtcgcatg acgccggaa gatttgaca 
      gccatagga attaagaaa ccgaatcat cggaaacgt 
      ctcaaagct gaaattgca caactcaat ctccccgac 
      aatttgcca gaattcatt ccgggttcg ctagaagag 
      tggttgcag ctgctgcgc ttggaggag tacctccct 
      gcatttctg gaccagggc tatcagacc gtcgacgac 
      gtgacgcag ctgacatgg gaggacttg gaagagttc 
      gggatagtt aagttaggc catcagaag aaaatcatg 
      ttagctata aaaagagtg aaagatatt agggcaggg 
      aagagaata aatacagat tcaaataga atttacgcg 
      acacaggac gtgatggtg cacgcaccc ttgggcccg 
      ccgtcgcct ggcgtcccc gcagtccac agcactttc 
      cgttctttc catcagccc tgggagatc gaccagagt 
      cgtcagctg gccgccggc acacactac gtgcaacca 
      atttattgc actgacata gttcccatc aagatacgg 
      acaggccgc ggcaagtcc ctggagtcc ctcgaggac 
      cccaacgag cgcacccac cacaccttc tccgccgaa 
      aacacccag accttctac taccagcaa cccatcggc 
      tggcggacg cggtcctac gacgacggc gacatcacc 
      cccaccaac gagacgggg ctgctgtac gagggcggc 
      ggcacgctg ccgcgcccc cgcggggtc atccgcccg 
      cgccccatc gccaagatc gcggccaag gcgcgcgag 
      agcttcccc gacttcgag aagccccag ttcaacccc 
      gagaagtac tccaacccg cagtacaag acgctgccc 
      cgggagctg atcgacacc accaagtac cacatacag 
      tacagcccc atgatgggc aagaaggtg ccccccaac 
      cccccgaag cggcaggac agcgagggg gccgagacc 
      accaccacg gtggagata caccacgtg cagacctcc 
      agtccgctg ccgctgccg gcgtacccc agctcggac 
      agcctgagt atatcgctg gacaaccag ggggacctg 
      ccgctgccg ccgcccccg gcgccgggc acgccgcca 
      gggaatgtg caatccaag atgaattcg tcgcagtcg 
      tggggcgct gaagaacag gaacttatc aaaactttg 
      gcgctgcag cacagaaac gggtccgat gcaagtttt 
      aaatcgagt tcaagcaca gagtcagat tcgctgcca 
      tttgccaat gagaacgcc ggtacgatt cgaactcgc 
      gcaggcggc aacagcaga ccggcggac tactccccc 
      gccaagccc cgcgccggt ctcccgccc catccgtcc 
      ccgacgccc aaccgacaa ccaagtgcc agaaatcag 
      agatccgaa cctgccgat gttcttaac gatatagga 
      aacatgctc gccaacttg accgacgaa ctagacgcg 
      atgttggaa gaagagaaa aggcaacag taa
    mRNA sequence
    • >TC002730 RA
      aaacttttt aacttatac agctagagt tgttttgtg 
      gcgaggatt ggtgtcgtt cctccccat ctgggaaac 
      aatacgtcc attagaggc cgcgtgtgt ggaatagcc 
      attagtgtc cgccctatg taaagtttt aagaccaca 
      caagtgctt aaattccct cagcgatat acgaaatgt 
      gcgtctcag tttccacaa aattgtaaa tcacagaaa 
      cacatgttc gtgtcttct attctggga ttgtcccgt 
      aattagtta tatttcccg tgttagcgg cagctgctc 
      taattgccc tgatccttg cgagtggaa atagagcga 
      tttttcgat gaatcattt cagagtgca agtgacaat 
      gcaatagtc ccgaaatgg caacgatct gtttccgac 
      ctccacctt tgcccccga acaagaaat cgcaattga 
      acatcaatc gttatcgga agcaaagtt tgctctcgt 
      cgacgtatc tgagtttcc gcgatccgg ccgaagacg 
      aggtcatac aagacgaaa tcgaactga cgaacttta 
      agtgtaaaa aaaagtgca aaaaagtgg cgcacacga 
      actcgctga gtcgggcca aggatgcaa aatgtgcca 
      tggtgcgga gccactccg acgggaacc tcaacaagg 
      agatagcgc ccatgacca tcaataggt acatcaagg 
      tgttgagcg gcagctgga agaatttgc tcaacttag 
      gcggcatgt cccggcccc cgaagccgg ccgccaccg 
      ccaagaagg tggctccac cggcggtgc cccacgagt 
      tccggcact cgggcagct ccttcgggt ccgcaggat 
      atgcctctt cggaggacg cgggcttcc tggcgccga 
      atgatcctc ccggagtcc cacgagacg atcattcgg 
      actatggca gcactgtca gcagcagca cgggcaagt 
      cccccggac ccatttttc cggcgccct ctttcacgt 
      ttccggggc agccgcccc ccggcggcg tcctcacgc 
      acaaggcgg ccgtctact accaccacc aactcagcc 
      tacaggagg atcagggta tcgacatga cacagtctc 
      cgggcaggg acagtccgg ggtcgtcga gcggaagtg 
      caggaagtg gctctcgcc acagcacgg cgtctttag 
      acagtggca gggcgtcgt accatccct tgagcacgg 
      caggaagga gtaccatcg ggtcctcga atagtccac 
      gatgttcct taagttctt gctcgatag gatcggagc 
      aaggccgaa ttgagcgaa tgatccatc aaggagttc 
      ctgaccaag aaattattc atgcgtggc tggttgatc 
      ttcagtacg atgaatatt tcccgttgt ttgtaagtg 
      ccggctacg atttgccga caataggtc gcatgacgc 
      cggaagatt tgacagcca taggaatta agaaaccga 
      atcatcgga aacgtctca aagctgaaa ttgcacaac 
      tcaatctcc ccgacaatt tgccagaat tcattccgg 
      gttcgctag aagagtggt tgcagctgc tgcgcttgg 
      aggagtacc tccctgcat ttctggacc agggctatc 
      agaccgtcg acgacgtga cgcagctga catgggagg 
      acttggaag agttcggga tagttaagt taggccatc 
      agaagaaaa tcatgttag ctataaaaa gagtgaaag 
      atattaggg cagggaaga gaataaata cagattcaa 
      atagaattt acgcgacac aggacgtga tggtgcacg 
      cacccttgg gcccgccgt cgcctggcg tccccgcag 
      tccacagca ctttccgtt ctttccatc agccctggg 
      agatcgacc agagtcgtc agctggccg ccggcacac 
      actacgtgc aaccaattt attgcactg acatagttc 
      ccatcaaga tacggacag gccgcggca agtccctgg 
      agtccctcg aggacccca acgagcgca cccaccaca 
      ccttctccg ccgaaaaca cccagacct tctactacc 
      agcaaccca tcggctggc ggacgcggt cctacgacg 
      acggcgaca tcaccccca ccaacgaga cggggctgc 
      tgtacgagg gcggcggca cgctgccgc gcccccgcg 
      gggtcatcc gcccgcgcc ccatcgcca agatcgcgg 
      ccaaggcgc gcgagagct tccccgact tcgagaagc 
      cccagttca accccgaga agtactcca acccgcagt 
      acaagacgc tgccccggg agctgatcg acaccacca 
      agtaccaca tacagtaca gccccatga tgggcaaga 
      aggtgcccc ccaaccccc cgaagcggc aggacagcg 
      agggggccg agaccacca ccacggtgg agatacacc 
      acgtgcaga cctccagtc cgctgccgc tgccggcgt 
      accccagct cggacagcc tgagtatat cgctggaca 
      accaggggg acctgccgc tgccgccgc ccccggcgc 
      cgggcacgc cgccaggga atgtgcaat ccaagatga 
      attcgtcgc agtcgtggg gcgctgaag aacaggaac 
      ttatcaaaa ctttggcgc tgcagcaca gaaacgggt 
      ccgatgcaa gttttaaat cgagttcaa gcacagagt 
      cagattcgc tgccatttg ccaatgaga acgccggta 
      cgattcgaa ctcgcgcag gcggcaaca gcagaccgg 
      cggactact cccccgcca agccccgcg ccggtctcc 
      cgccccatc cgtccccga cgcccaacc gacaaccaa 
      gtgccagaa atcagagat ccgaacctg ccgatgttc 
      ttaacgata taggaaaca tgctcgcca acttgaccg 
      acgaactag acgcgatgt tggaagaag agaaaaggc 
      aacagtaac tcgaagcat aagactgag cctatttta 
      tcctaattt tagttatcg gaagcttta actataaat 
      atgacctcc attcgggtt gtgtttgtc gttgacgag 
      tttatttca tagaactga ctattgggt agattttcg 
      ttactagtt tcctaagtt attgtgtaa atagaatgc 
      tttattttc gattacaaa caaggtttg taaatattt 
      ttagataga gtttttata ttgtaaaga ttgtaaaat 
      ttatcagtt cttttctat ggtgtcatt atagctttc 
      agcaggtac tgttcgcct aatttcttc ggaacggag 
      tggaatgcg tgttctggg tgattccgt tttaactta 
      accttctga ttgaggcaa tactttcaa agcaattcg 
      gcatcttcc gttcgagag aatattatt aatcaaaaa 
      ctacttcag ttggtgagt gtaaataga tagatcttg 
      tactgaaga cagcaaata ggaaagcaa gtaataact 
      atcaaatta gagaataat tttgtacaa agccatcat 
      ttagtatac ggaaatagc gagttagtc taaatcagt 
      tttggtgtt ttaatacct ttacaccaa tttgcaatg 
      ttagtctca tttgtttct aacaaatgg ttgaaaaag 
      actggtagt tcgattaca aaattctca atttcctgt 
      catgtatct cgtgccata tccaatatt agcacactt 
      gattgtaat taaatttag ttgcgacgt gaaaactga 
      tattttaac tgtacatga cacgtaatt gatcgaaaa 
      tcttctgtg ttcaatata atttacgat ttatatgtt 
      tgtaaaaaa tgttattta atgtgctac ttaaaatag 
      ctatcattt cggattctc ataataatt attatttgt 
      tatttgatt tccattaca ttgcattat taattaatc 
      tagctgtta attccattg t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY