Status
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Gene information
General information
TC032495
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002715,TC002714
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid18: 134329..152464
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032495
    iBeetle screen iB_03441
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    iBeetle screen iB_03442
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032495 RA
      MKAYLERAK EHEEFMKEQ TVEFQIGKR HLANMMGED 
      PESFTQEDI DSAIEYLFP SGLFEKKAR PFMRPPEEV 
      FPQRKAAEF DETGRPYNF LFYTGKANY YNLMHDIVE 
      HINECYKFE DSMIRKGVK YDPTLQLDL SGYQWLDKP 
      SLEQELVET IGDKDYESF VATMERLCS LPYSYRFKD 
      FIFKYRKPL MTHEQTYQA PKPQFDADG RAFITTYEC 
      LRKRARGHV TIRSPGTGK ISINGEDIS YFKETQGRE 
      QLYLELREF PNIPTSIIA VCGSVCACP SCVPSVIVI 
      QASFPSFVQ FGSPLLNPP TVVIVHFNN HTTKSLKMD 
      NMGFQDSTN SRKLGRSVS VNAEVESFP LYKTHTCPS 
      GLRWRRPRD DPEIVVEEE FPSDDFEYM ECGPSPPAD 
      HTPNIYESF EEFSRSELT VQICHDTIK MNLLEHMKM 
      ASGRHQLLD DIECKKTTQ DTLTEAFQG ATKLEINIA 
      FLWAAFMKR WELLDGLLK LGAQLRYYE PSQGLSALH 
      LASFSGCIP GTQFLLAQG CDVNAIFKC YTPLHCSAF 
      GDSPETAMI LLNNGAKVQ ALTNSPNNC HESVLHCAV 
      RANAIACVR LFTAEGADV GQFEFSGMS PVHLAADLG 
      HPQCLKIML EAKGVNVNA KTKEKELTP LHLAAEGGY 
      VECVEILLD KGADANIRN HRGQTPLHL AARAQAYDC 
      VEMLLRKGN ADPNIGDFD KRTPLHAAV CKAARSYDI 
      IEILVSWGA DVNTKDQYG YTPLHIAAL NELSQCVEI 
      LIYHGADVT AKSKFGMTA LGIITRKTP ASLAMVTHK 
      LDSAITLHH HPESSNREV ELRLDFRSI LQHCHPREI 
      SFLNTFVDE GQKEILLHP LCSAFLYLK WEKIRKYYI 
      ARMLFCFIF VLSLSLYVL TALAHNCYN HGKNMNDTQ 
      PQNVIELCE KKSMMGHML RTNPFVIEM QWFVLVGIT 
      CCEILRKVY GIAGYPTVK QYLSHPENI IEWTVVVSV 
      FVISFIYTG RTYTWQNHI GAFAVLFGW TNLMLMIGQ 
      LPVFGSYVA MYTRVQGEF AKLLLAYSC LLIGFTISF 
      CVIFPDSST FANPFIGLI TVLVMMTGE LNLDLLVDD 
      DPEDPPFLL EISAQVTYI LFLLFVTVI LMNLLVGIA 
      VHDIQGLQK TAGLSKLVR QTKLISYME LALFNGYLP 
      RYLLNLLHW TALVSPKAY RVVLNVKPL NPREKRLPR 
      DILKAAHEI AKKRKHYAH TISSHGSNA TTRKIINNN 
      MDQNSEILE YPSAFPLLQ SKIEKSTEQ IEHLSKEVR 
      ELKIALQNN QKIMEQLLH GLVNTKNSS NC
    CDS sequence
    • >TC032495 RA
      atgaaagca taccttgaa agagctaaa gaacatgaa 
      gaattcatg aaagaacaa actgtagaa tttcaaatt 
      ggaaaacga catttggcg aatatgatg ggggaagat 
      cctgaatcg ttcacccag gaagacatt gatagcgcg 
      atagagtat ttattcccg tcgggatta tttgagaaa 
      aaagcgaga ccttttatg cgccctcca gaggaggtt 
      tttccacag aggaaagct gcagagttt gatgaaacc 
      ggacgtcct tacaacttc ttgttttac acaggaaaa 
      gcaaattac tataatctt atgcatgat atcgttgag 
      catattaat gagtgctac aagtttgaa gattccatg 
      ataagaaaa ggggttaaa tatgacccc actttgcaa 
      ctggatttg tctggatat caatggcta gataagccc 
      tctttagaa caagaactt gtagaaaca attggcgat 
      aaagactat gaaagtttt gttgctacc atggagaga 
      ctctgcagt ctaccctac tcttacagg ttcaaagat 
      tttattttc aagtataga aaaccctta atgacccat 
      gaacaaacg taccaagct cccaaacct caatttgat 
      gctgacgga agagcattt ataacaact tatgaatgt 
      ctgaggaag agagctcgc ggccacgtg accattcgt 
      tctcccggc accggtaaa atttccata aacggtgag 
      gacatctca tactttaaa gaaactcag ggcagagaa 
      cagttgtat ctcgaattg agggaattc ccgaacatt 
      ccgacctca attatcgcg gtctgtggc agcgtctgt 
      gcctgtcct agttgcgtc ccttccgtc attgtcatt 
      caagcttct tttccttcg ttcgtccaa ttcgggtca 
      ccattgctc aatcctccg actgttgtg atagtccac 
      tttaataac cacaccacg aaatcccta aaaatggac 
      aatatgggt ttccaagac tccaccaac tcccgcaaa 
      ctgggccgc tccgtcagc gtgaacgcc gaagtggag 
      agcttcccg ttgtacaaa acacacacg tgcccctcg 
      ggcctccgg tggcggcgc ccccgcgac gacccggag 
      atcgtcgtc gaggaggag ttcccctcc gacgacttc 
      gagtacatg gaatgcggc ccttcgccg cccgccgac 
      cacacgccc aacatctac gaaagcttc gaggagttc 
      tcccggagc gagctcaca gtgcagatc tgccacgac 
      acgatcaag atgaacctc ctcgaacac atgaagatg 
      gcctccggc cgccaccaa ctcctcgac gacatcgag 
      tgcaaaaaa accacgcag gacaccctc accgaagcg 
      ttccaaggc gccaccaag ctggaaatc aacatcgcg 
      tttttgtgg gccgctttc atgaaacgc tgggagctg 
      ctggatggg ctcctgaaa ctgggggcc caactgcgc 
      tactacgag cccagtcag gggctcagc gccctgcat 
      ttggcctcg ttcagtggg tgcatcccc gggacgcag 
      ttcctgcta gcccaaggg tgtgacgtc aacgcgatt 
      ttcaagtgt tacactccg ttgcattgt tcggcgttc 
      ggggacagt cccgaaaca gcgatgatt ttgttgaat 
      aacggggcg aaagtccaa gctttgacc aacagtccg 
      aataactgc cacgagagt gtgctacat tgtgcggtt 
      agggcgaat gccatcgcg tgcgttcgg ttgtttacg 
      gcggagggg gccgatgtg gggcagttc gagttttcg 
      gggatgtcc ccagtgcat ctcgcggcc gatttgggg 
      cacccacag tgtcttaaa atcatgctg gaggcgaag 
      ggtgttaac gtgaacgcc aaaacaaaa gagaaggaa 
      ttaacgccg ttgcatcta gccgccgag ggtggttat 
      gtcgagtgt gttgagata cttctggac aagggggct 
      gatgcgaat atacggaac catcggggg cagacgccg 
      ctccatctg gcggcgcgg gcgcaagct tacgattgt 
      gtcgagatg ctcctgagg aagggcaac gccgatccg 
      aatatcggt gatttcgac aagagaacg cctctgcat 
      gctgctgtc tgcaaggcg gctagatcg tacgacata 
      atcgagatt ctggtcagt tggggggca gatgtcaac 
      accaaagac cagtacggt tacacccct ctacacata 
      gccgccttg aacgaactg tcacaatgt gtggaaatc 
      ctgatctat cacggggcc gacgtgacg gccaaatcc 
      aaattcgga atgacggcg ctgggtatc atcacgcgc 
      aaaacccca gcgtccctt gccatggtc acccataaa 
      ctcgactcg gctataact ctccatcac caccccgaa 
      tcctccaac cgcgaggtg gagttgcgc ctcgacttc 
      aggagcatc ctccagcat tgccacccc cgggagatc 
      agcttcctc aatacgttc gtcgacgaa ggccagaag 
      gagattctc ttgcacccg ctctgctcc gcgtttctc 
      tacctcaaa tgggagaag atccgcaag tactacatc 
      gcccgcatg ttgttttgt ttcatcttc gttctgagt 
      ttgtctctg tacgtgttg acggctctg gcccacaat 
      tgctacaac catggtaaa aacatgaac gatacccag 
      ccgcagaac gtgatcgag ttgtgcgag aagaagtcc 
      atgatgggc cacatgttg cggactaat ccgttcgtt 
      atcgaaatg cagtggttc gtgctggtg ggcatcacc 
      tgttgcgag atcctgagg aaggtgtac gggatcgcg 
      ggctatccc actgtcaag cagtacctc tcgcacccc 
      gagaatata atcgagtgg actgttgtt gttagtgtg 
      tttgttatt tctttcatc tacaccggg agaacgtac 
      acttggcag aaccacatt ggggcgttt gccgtgttg 
      ttcggttgg accaatctc atgctgatg attggccag 
      ttgccagtg ttcggctcc tacgttgct atgtatacg 
      agagtacaa ggagaattt gccaaatta ctgttagcc 
      tactcatgt ttattgata ggttttacg ataagtttt 
      tgtgtgatc ttcccggac tcgtccacg ttcgccaat 
      cccttcatc ggccttatc accgtcctt gtgatgatg 
      acgggcgaa ctgaacctg gacctgctg gttgacgac 
      gacccggag gacccccct ttcctctta gaaataagt 
      gcgcaagtc acctacatc ctcttcctc ctgtttgtc 
      actgttatt ttaatgaac ctgctggtg ggtatcgcc 
      gtccacgat atacaaggc ttgcagaaa acggccggc 
      ttgtccaag ctggtccgt cagaccaaa ttgatttcg 
      tacatggag ttggcgctg tttaacggc tatttgccg 
      agatatctc ctcaatttg ctgcattgg acggctttg 
      gtgtccccg aaggcgtac agggttgtc ctgaatgtg 
      aagccactg aatccgagg gagaaacgc ctcccgagg 
      gatatacta aaagcggcg catgaaatc gccaaaaag 
      agaaaacac tacgcacat acgatttcc tcacatggc 
      agtaacgct actactagg aaaattatt aacaataat 
      atggaccag aatagtgag attttggaa tatccaagt 
      gcctttcct ttacttcaa agtaaaatt gagaaaagt 
      accgagcaa attgaacat ttaagtaaa gaggttagg 
      gagttgaag attgctctt caaaacaac cagaagatt 
      atggaacag ttgttgcat ggcttagtg aatacgaag 
      aatagctca aattgttag
    mRNA sequence
    • >TC032495 RA
      tggcaatgg tgtcggaaa gaagttgag gttagaaag 
      tgaattaaa tgtgaattt tatttttta tgtaaaaag 
      tatgttgcg acgaattaa cgtggttgt aaaaccatt 
      ggcggaaca tctgcattt tcaaaacaa tcaaaggat 
      aatttcgtc atgtaatat taaggcgcg acaattttg 
      tacacctgc ccctaatac agtaccgca gtctaagaa 
      aactataag caaggcaat gaaagcata ccttgaaag 
      agctaaaga acatgaaga attcatgaa agaacaaac 
      tgtagaatt tcaaattgg aaaacgaca tttggcgaa 
      tatgatggg ggaagatcc tgaatcgtt cacccagga 
      agacattga tagcgcgat agagtattt attcccgtc 
      gggattatt tgagaaaaa agcgagacc ttttatgcg 
      ccctccaga ggaggtttt tccacagag gaaagctgc 
      agagtttga tgaaaccgg acgtcctta caacttctt 
      gttttacac aggaaaagc aaattacta taatcttat 
      gcatgatat cgttgagca tattaatga gtgctacaa 
      gtttgaaga ttccatgat aagaaaagg ggttaaata 
      tgaccccac tttgcaact ggatttgtc tggatatca 
      atggctaga taagccctc tttagaaca agaacttgt 
      agaaacaat tggcgataa agactatga aagttttgt 
      tgctaccat ggagagact ctgcagtct accctactc 
      ttacaggtt caaagattt tattttcaa gtatagaaa 
      acccttaat gacccatga acaaacgta ccaagctcc 
      caaacctca atttgatgc tgacggaag agcatttat 
      aacaactta tgaatgtct gaggaagag agctcgcgg 
      ccacgtgac cattcgttc tcccggcac cggtaaaat 
      ttccataaa cggtgagga catctcata ctttaaaga 
      aactcaggg cagagaaca gttgtatct cgaattgag 
      ggaattccc gaacattcc gacctcaat tatcgcggt 
      ctgtggcag cgtctgtgc ctgtcctag ttgcgtccc 
      ttccgtcat tgtcattca agcttcttt tccttcgtt 
      cgtccaatt cgggtcacc attgctcaa tcctccgac 
      tgttgtgat agtccactt taataacca caccacgaa 
      atccctaaa aatggacaa tatgggttt ccaagactc 
      caccaactc ccgcaaact gggccgctc cgtcagcgt 
      gaacgccga agtggagag cttcccgtt gtacaaaac 
      acacacgtg cccctcggg cctccggtg gcggcgccc 
      ccgcgacga cccggagat cgtcgtcga ggaggagtt 
      cccctccga cgacttcga gtacatgga atgcggccc 
      ttcgccgcc cgccgacca cacgcccaa catctacga 
      aagcttcga ggagttctc ccggagcga gctcacagt 
      gcagatctg ccacgacac gatcaagat gaacctcct 
      cgaacacat gaagatggc ctccggccg ccaccaact 
      cctcgacga catcgagtg caaaaaaac cacgcagga 
      caccctcac cgaagcgtt ccaaggcgc caccaagct 
      ggaaatcaa catcgcgtt tttgtgggc cgctttcat 
      gaaacgctg ggagctgct ggatgggct cctgaaact 
      gggggccca actgcgcta ctacgagcc cagtcaggg 
      gctcagcgc cctgcattt ggcctcgtt cagtgggtg 
      catccccgg gacgcagtt cctgctagc ccaagggtg 
      tgacgtcaa cgcgatttt caagtgtta cactccgtt 
      gcattgttc ggcgttcgg ggacagtcc cgaaacagc 
      gatgatttt gttgaataa cggggcgaa agtccaagc 
      tttgaccaa cagtccgaa taactgcca cgagagtgt 
      gctacattg tgcggttag ggcgaatgc catcgcgtg 
      cgttcggtt gtttacggc ggagggggc cgatgtggg 
      gcagttcga gttttcggg gatgtcccc agtgcatct 
      cgcggccga tttggggca cccacagtg tcttaaaat 
      catgctgga ggcgaaggg tgttaacgt gaacgccaa 
      aacaaaaga gaaggaatt aacgccgtt gcatctagc 
      cgccgaggg tggttatgt cgagtgtgt tgagatact 
      tctggacaa gggggctga tgcgaatat acggaacca 
      tcgggggca gacgccgct ccatctggc ggcgcgggc 
      gcaagctta cgattgtgt cgagatgct cctgaggaa 
      gggcaacgc cgatccgaa tatcggtga tttcgacaa 
      gagaacgcc tctgcatgc tgctgtctg caaggcggc 
      tagatcgta cgacataat cgagattct ggtcagttg 
      gggggcaga tgtcaacac caaagacca gtacggtta 
      cacccctct acacatagc cgccttgaa cgaactgtc 
      acaatgtgt ggaaatcct gatctatca cggggccga 
      cgtgacggc caaatccaa attcggaat gacggcgct 
      gggtatcat cacgcgcaa aaccccagc gtcccttgc 
      catggtcac ccataaact cgactcggc tataactct 
      ccatcacca ccccgaatc ctccaaccg cgaggtgga 
      gttgcgcct cgacttcag gagcatcct ccagcattg 
      ccacccccg ggagatcag cttcctcaa tacgttcgt 
      cgacgaagg ccagaagga gattctctt gcacccgct 
      ctgctccgc gtttctcta cctcaaatg ggagaagat 
      ccgcaagta ctacatcgc ccgcatgtt gttttgttt 
      catcttcgt tctgagttt gtctctgta cgtgttgac 
      ggctctggc ccacaattg ctacaacca tggtaaaaa 
      catgaacga tacccagcc gcagaacgt gatcgagtt 
      gtgcgagaa gaagtccat gatgggcca catgttgcg 
      gactaatcc gttcgttat cgaaatgca gtggttcgt 
      gctggtggg catcacctg ttgcgagat cctgaggaa 
      ggtgtacgg gatcgcggg ctatcccac tgtcaagca 
      gtacctctc gcaccccga gaatataat cgagtggac 
      tgttgttgt tagtgtgtt tgttatttc tttcatcta 
      caccgggag aacgtacac ttggcagaa ccacattgg 
      ggcgtttgc cgtgttgtt cggttggac caatctcat 
      gctgatgat tggccagtt gccagtgtt cggctccta 
      cgttgctat gtatacgag agtacaagg agaatttgc 
      caaattact gttagccta ctcatgttt attgatagg 
      ttttacgat aagtttttg tgtgatctt cccggactc 
      gtccacgtt cgccaatcc cttcatcgg ccttatcac 
      cgtccttgt gatgatgac gggcgaact gaacctgga 
      cctgctggt tgacgacga cccggagga ccccccttt 
      cctcttaga aataagtgc gcaagtcac ctacatcct 
      cttcctcct gtttgtcac tgttatttt aatgaacct 
      gctggtggg tatcgccgt ccacgatat acaaggctt 
      gcagaaaac ggccggctt gtccaagct ggtccgtca 
      gaccaaatt gatttcgta catggagtt ggcgctgtt 
      taacggcta tttgccgag atatctcct caatttgct 
      gcattggac ggctttggt gtccccgaa ggcgtacag 
      ggttgtcct gaatgtgaa gccactgaa tccgaggga 
      gaaacgcct cccgaggga tatactaaa agcggcgca 
      tgaaatcgc caaaaagag aaaacacta cgcacatac 
      gatttcctc acatggcag taacgctac tactaggaa 
      aattattaa caataatat ggaccagaa tagtgagat 
      tttggaata tccaagtgc ctttccttt acttcaaag 
      taaaattga gaaaagtac cgagcaaat tgaacattt 
      aagtaaaga ggttaggga gttgaagat tgctcttca 
      aaacaacca gaagattat ggaacagtt gttgcatgg 
      cttagtgaa tacgaagaa tagctcaaa ttgttagaa 
      atgtacaga ctgtgatgt attttatat tagatttta 
      atttaatga tataagtaa atatacgta aaagatgtt 
      a