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Gene information
General information
TC002711 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 13599945..13605052
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002711
    iBeetle screen iB_03440

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002711 RA
      MSDYVDVKC RIKLKRRIV DNMLKGKVE LIASDVIVI 
      KNAYIEITG FASCSWNLD AVYIMFPKV YPEDTVVKG 
      FQQFYYTRH ALCGGEIAH EHKPNEAYK FKCYWPSRL 
      PGTWFGDFG NIEYIAKVT IKLDNELDV YTHSQLIYV 
      KFRRHLATE KHLLFEGRV DVSRNFFTT KSIGNVIVS 
      VWLPISGIA AGQSLPVIC SVTNHSKLH FGSLIFVLT 
      RIDIYRSDT PISLVKTVR TDVCRVARM VNIHKGKYD 
      FYSVLQTDT GTIPTNQFY KCTCCQVIY EVWVSVQGA 
      FRRYRSFGY PNIDTPGIP VIIGTKPLR NFNTYEITA 
      EIFKKMYET HLPVLFDEL PEDQRRYTE FGKATTR
    CDS sequence
    • >TC002711 RA
      atgagcgat tacgtagac gtcaagtgc agaattaaa 
      ctaaagcgg cgtattgtg gataatatg ctaaaaggc 
      aaagtcgaa ttaatagca tctgatgtt attgttatt 
      aagaatgca tacattgag ataacagga ttcgcatct 
      tgtagctgg aatctggat gcagtttat atcatgttt 
      cctaaagta taccccgaa gatactgtt gtgaaaggc 
      ttccagcaa ttttattat acgcgtcac gcgctgtgt 
      ggtggagag atagcacat gagcacaaa ccgaatgaa 
      gcttataaa tttaaatgc tattggccg tcacgttta 
      ccgggaact tggtttggc gattttggg aatattgaa 
      tatatcgct aaagtcacg attaaatta gacaacgag 
      ttggatgtt tatactcac tcccaactc atctatgta 
      aaatttcgg agacaccta gctaccgaa aaacacctt 
      ctgtttgag ggaagagtc gacgtctcg cgcaatttc 
      ttcacaacg aaatcaatt ggaaatgtt attgtgagt 
      gtttggctg ccgatttct ggaattgct gccggacag 
      tctctacca gtgatctgc tccgttacc aatcactcc 
      aaattacat ttcggaagt cttatcttc gttctgaca 
      agaatcgac atttacaga agcgacacc cctatatct 
      ttggtaaag acagttcgg acggacgtg tgccgcgtc 
      gcccgaatg gtcaatatt cacaaaggc aagtacgat 
      ttctactca gttctacaa actgacaca ggcactatt 
      cccacaaac caattctac aaatgcact tgttgccaa 
      gttatttac gaagtgtgg gtctcggtt caaggcgct 
      tttaggcgc tataggagc ttcgggtac cccaatatc 
      gataccccg ggtattccg gtcataatt ggaacaaag 
      cccttgcga aacttcaat acgtatgaa ataacagca 
      gagattttc aagaagatg tatgaaact catctacct 
      gttctgttt gatgagttg cctgaggat cagagacgc 
      tatacagaa tttggaaag gcaactaca cgttaa
    mRNA sequence
    • >TC002711 RA
      tgatttgta ccacgctcg gtcagaaat taaacttat 
      gtgcccctt tatttagga atatcaacc aaatgaaat 
      aatttccaa aactctaag tttatatca cttgtgttt 
      tcgaccaaa gatcgaaat atgatcgaa atcactagg 
      cgaagcggg aaaatataa acgtaaaaa atgagcgat 
      tacgtagac gtcaagtgc agaattaaa ctaaagcgg 
      cgtattgtg gataatatg ctaaaaggc aaagtcgaa 
      ttaatagca tctgatgtt attgttatt aagaatgca 
      tacattgag ataacagga ttcgcatct tgtagctgg 
      aatctggat gcagtttat atcatgttt cctaaagta 
      taccccgaa gatactgtt gtgaaaggc ttccagcaa 
      ttttattat acgcgtcac gcgctgtgt ggtggagag 
      atagcacat gagcacaaa ccgaatgaa gcttataaa 
      tttaaatgc tattggccg tcacgttta ccgggaact 
      tggtttggc gattttggg aatattgaa tatatcgct 
      aaagtcacg attaaatta gacaacgag ttggatgtt 
      tatactcac tcccaactc atctatgta aaatttcgg 
      agacaccta gctaccgaa aaacacctt ctgtttgag 
      ggaagagtc gacgtctcg cgcaatttc ttcacaacg 
      aaatcaatt ggaaatgtt attgtgagt gtttggctg 
      ccgatttct ggaattgct gccggacag tctctacca 
      gtgatctgc tccgttacc aatcactcc aaattacat 
      ttcggaagt cttatcttc gttctgaca agaatcgac 
      atttacaga agcgacacc cctatatct ttggtaaag 
      acagttcgg acggacgtg tgccgcgtc gcccgaatg 
      gtcaatatt cacaaaggc aagtacgat ttctactca 
      gttctacaa actgacaca ggcactatt cccacaaac 
      caattctac aaatgcact tgttgccaa gttatttac 
      gaagtgtgg gtctcggtt caaggcgct tttaggcgc 
      tataggagc ttcgggtac cccaatatc gataccccg 
      ggtattccg gtcataatt ggaacaaag cccttgcga 
      aacttcaat acgtatgaa ataacagca gagattttc 
      aagaagatg tatgaaact catctacct gttctgttt 
      gatgagttg cctgaggat cagagacgc tatacagaa 
      tttggaaag gcaactaca cgttaatga acaattaat 
      aagttcaat caaacaatc aagctcaca aaaactcaa 
      aatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY