Status
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Gene information
General information
TC002698 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 14005912..14024249
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002698
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03439

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002698 RA
      MAPIGDGPE YDLVVIGGG SGGLAAAKE AAGLGAKVA 
      VLDYVTPSP KGTKWGLGG TCVNVGCIP KKLMHQAAL 
      LGEAIEDAK SYGWQFPQP ENIKHDWAS LRQAVQNHI 
      KSVNWVTRV ELRDKKVEY INGLGVFKD PHTVHTVTK 
      QGERTLTSK YFLIAVGGR PRYPNIPGA VEYGITSDD 
      IFSLEEAPG KTLVVGAGY IGLECAGFL RGLGYDATV 
      MVRSVVLRG FDQQMAKLI ASAMEEKGV KFLHKCLPK 
      SVEKRSDNK LLVKWSNET GQEFEDVFD TVLFAIGRR 
      ALTRELHLD KAGVKVAGD GEKIDAMNE QSNVPHIFA 
      VGDVLYKKP ELTPVAIHA GRLLARRLF GNSTVQMDY 
      DNVATTVFS PLEYGSVGI SEETAIQRF GENNIEIYH 
      AYYKPTEFF IPQRSIAHC YLKVVAKRE GPQQVLGMH 
      FIGPQAGEV IQGFAAAMK CNLTVNALM STVGIHPTI 
      AEEFTRINI TKRSGKDPN PASCCS
    CDS sequence
    • >TC002698 RA
      atggcacca ataggtgac ggtccagaa tatgacttg 
      gtagtaatt ggaggtgga tctggtgga cttgcagct 
      gctaaagaa gcagctggg ttaggtgcc aaagttgca 
      gttttagat tatgtaaca ccatcacct aaaggtact 
      aaatggggt cttggtggt acctgtgtc aatgttgga 
      tgtataccg aaaaaattg atgcatcaa gctgcttta 
      ctgggagag gctatcgag gatgctaag agttacggt 
      tggcaattt ccccaacca gaaaacatc aagcacgat 
      tgggcgtct ctcagacag gctgtgcaa aatcatatt 
      aagtctgtc aattgggtg accagggta gaacttaga 
      gataaaaag gtagaatac atcaatggt ttgggcgtt 
      tttaaagat cctcacacc gttcatacg gtgaccaaa 
      cagggagaa cgtacatta acatccaag tattttctg 
      atagcagta ggtggaaga cctcgttat cctaatata 
      ccgggtgcc gtagaatac ggtattacc agtgatgat 
      attttcagt ttggaggag gcaccggga aaaacacta 
      gttgtcggt gctggatat attggtttg gaatgtgcc 
      ggttttttg agaggatta ggttatgac gctaccgtc 
      atggtccga tctgtggtc ttgcgaggt ttcgaccaa 
      cagatggct aaactcatc gcttcagca atggaggag 
      aaaggagtt aaattcctt cacaagtgt ctgccaaaa 
      tccgtcgaa aagcgatct gataacaag ctacttgtt 
      aaatggtca aatgagact ggtcaagaa ttcgaagat 
      gtctttgat accgtattg tttgcaatt ggtagaaga 
      gctcttaca agggaactt catttggat aaggcaggt 
      gttaaagtg gctggagat ggtgagaag atagatgcg 
      atgaatgaa cagtcaaat gtacctcac atatttgcc 
      gtcggagat gttttatat aaaaagccc gaattgact 
      ccagtggct atccacgct ggacgtctc ttggcccgc 
      cggttattc ggcaacagc actgtccaa atggactac 
      gacaacgtg gcaacaact gttttcagt cctctggaa 
      tatggaagt gttggcata agtgaagaa acagcaatt 
      cagcgtttc ggcgaaaac aacatcgaa atctatcac 
      gcgtactac aaaccaacc gaatttttc attccgcaa 
      cgttcgatc gctcattgc tatttgaaa gttgtggcg 
      aaacgtgaa gggcctcag caagttttg ggaatgcac 
      tttattgga ccacaagct ggggaagtt attcaagga 
      ttcgcagcg gctatgaag tgcaacttg actgtgaat 
      gcgttgatg agcactgtc ggcatacat ccaacaatt 
      gcggaagag tttacaagg attaacatt acgaagagg 
      tccgggaaa gacccgaac ccggcgtct tgctgcagt 
      taa
    mRNA sequence
    • >TC002698 RA
      acacaaata gttttccgc gcttgtcac tgtcacatt 
      ttgcgctgg cgtacatcg aaacatccg tacaaatag 
      ttttaagtt aattaaatt cgtgttctg taattattt 
      gaacgaaca ttaacgcac tgatcagtt gtgtttgtc 
      ggtgccgcc gcaaaaaaa accaacgtt tcctgtttt 
      aactcaatt cctgtgcaa agtttcatt cgtgaatcg 
      gaatggcac caataggtg acggtccag aatatgact 
      tggtagtaa ttggaggtg gatctggtg gacttgcag 
      ctgctaaag aagcagctg ggttaggtg ccaaagttg 
      cagttttag attatgtaa caccatcac ctaaaggta 
      ctaaatggg gtcttggtg gtacctgtg tcaatgttg 
      gatgtatac cgaaaaaat tgatgcatc aagctgctt 
      tactgggag aggctatcg aggatgcta agagttacg 
      gttggcaat ttccccaac cagaaaaca tcaagcacg 
      attgggcgt ctctcagac aggctgtgc aaaatcata 
      ttaagtctg tcaattggg tgaccaggg tagaactta 
      gagataaaa aggtagaat acatcaatg gtttgggcg 
      tttttaaag atcctcaca ccgttcata cggtgacca 
      aacagggag aacgtacat taacatcca agtattttc 
      tgatagcag taggtggaa gacctcgtt atcctaata 
      taccgggtg ccgtagaat acggtatta ccagtgatg 
      atattttca gtttggagg aggcaccgg gaaaaacac 
      tagttgtcg gtgctggat atattggtt tggaatgtg 
      ccggttttt tgagaggat taggttatg acgctaccg 
      tcatggtcc gatctgtgg tcttgcgag gtttcgacc 
      aacagatgg ctaaactca tcgcttcag caatggagg 
      agaaaggag ttaaattcc ttcacaagt gtctgccaa 
      aatccgtcg aaaagcgat ctgataaca agctacttg 
      ttaaatggt caaatgaga ctggtcaag aattcgaag 
      atgtctttg ataccgtat tgtttgcaa ttggtagaa 
      gagctctta caagggaac ttcatttgg ataaggcag 
      gtgttaaag tggctggag atggtgaga agatagatg 
      cgatgaatg aacagtcaa atgtacctc acatatttg 
      ccgtcggag atgttttat ataaaaagc ccgaattga 
      ctccagtgg ctatccacg ctggacgtc tcttggccc 
      gccggttat tcggcaaca gcactgtcc aaatggact 
      acgacaacg tggcaacaa ctgttttca gtcctctgg 
      aatatggaa gtgttggca taagtgaag aaacagcaa 
      ttcagcgtt tcggcgaaa acaacatcg aaatctatc 
      acgcgtact acaaaccaa ccgaatttt tcattccgc 
      aacgttcga tcgctcatt gctatttga aagttgtgg 
      cgaaacgtg aagggcctc agcaagttt tgggaatgc 
      actttattg gaccacaag ctggggaag ttattcaag 
      gattcgcag cggctatga agtgcaact tgactgtga 
      atgcgttga tgagcactg tcggcatac atccaacaa 
      ttgcggaag agtttacaa ggattaaca ttacgaaga 
      ggtccggga aagacccga acccggcgt cttgctgca 
      gttaaaaac actatgagt aatagaatc acttagaac 
      ggattggct ccagtgata ttacttatc ttgaaacgg 
      ccacccaag gtcaatttt tttcttcta gtataatta 
      tttgtaagt atacattcc acagtgcct ttacatttt 
      taagtgaaa tgtatacat actcaatta cacaacgtt 
      taatcagta tcaagaccc attgtacct taatattta 
      attttatta cactttttt gttgtaacc a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY