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Gene information
General information
TC002663 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17587565..17588552
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002663
    iBeetle screen iB_03435

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002663 RA
      MGVWGNLYS DSLSKLDQV TDLKILANN LSEIDSGAI 
      CANPSLKKF ELVLGPETS LQKLTKSHF HNCQNLEEV 
      SIVGIYKNS STSIEKGAF EDLTNLKNL NLANLEIPH 
      LTHNFFKNL NSLTHLSVR KCGIEKLES GVFDDLKNI 
      EEIAIFFNK FTTLPENLF KNNGKAKRV VLFSNNITD 
      LTWDEFAGL DSVTDLLIG RNSVAHFDA GKIAKHMPQ 
      LRELNMELN PISCEEIEQ FAKELTSKL NHTVKVTHM 
      SDPSAPNSC IYQ
    CDS sequence
    • >TC002663 RA
      atgggtgtt tggggtaac ctttactcc gattcattg 
      tcaaaactg gaccaagta actgatctc aaaattctt 
      gcaaataat ttaagcgaa atcgattca ggagcgatt 
      tgtgcaaac ccaagtttg aaaaaattt gagttagtg 
      ttaggccct gaaacaagt ctccaaaaa ctgacaaaa 
      agtcacttc cacaattgc cagaatttg gaagaagtt 
      tcaatcgtt ggtatttac aaaaactca tcgacttcg 
      atcgaaaaa ggagctttc gaggacttg accaacttg 
      aagaatctc aatcttgca aatttggag attccacat 
      ttgacacac aattttttc aaaaacctg aactctttg 
      acacactta tcagtgagg aaatgcggg attgaaaaa 
      ctcgagtct ggtgttttt gacgatttg aagaacatt 
      gaagaaatt gcgattttc ttcaacaag tttacaacc 
      ctgccggag aatttgttc aaaaacaac ggcaaagcg 
      aagcgggtg gttttgttt tcgaacaat atcactgat 
      ttgacgtgg gacgagttc gcagggctg gactcagtg 
      accgatctt ttgattggg cgcaattca gtcgcacat 
      ttcgacgct ggaaaaata gcgaaacac atgccacaa 
      ttgagagag cttaacatg gagctaaac ccgatttcg 
      tgcgaagaa atcgaacaa ttcgccaag gaattgacg 
      tcgaagttg aaccacact gtgaaagtc acacacatg 
      tccgatccc tcagccccc aattcttgt atttaccaa 
      taa
    mRNA sequence
    • >TC002663 RA
      actaattta tttttttta gaatttttt gctacttgt 
      tttggcaaa gtgtgttca atcgcgtga catttgggg 
      atgggaagt gctatcaga agtctataa tgtcacact 
      aactgaagg aaaaccttt agaaattaa tagaacttt 
      gacaaaagt ccagctaat gggtgtttg gggtaacct 
      ttactccga ttcattgtc aaaactgga ccaagtaac 
      tgatctcaa aattcttgc aaataattt aagcgaaat 
      cgattcagg agcgatttg tgcaaaccc aagtttgaa 
      aaaatttga gttagtgtt aggccctga aacaagtct 
      ccaaaaact gacaaaaag tcacttcca caattgcca 
      gaatttgga agaagtttc aatcgttgg tatttacaa 
      aaactcatc gacttcgat cgaaaaagg agctttcga 
      ggacttgac caacttgaa gaatctcaa tcttgcaaa 
      tttggagat tccacattt gacacacaa ttttttcaa 
      aaacctgaa ctctttgac acacttatc agtgaggaa 
      atgcgggat tgaaaaact cgagtctgg tgtttttga 
      cgatttgaa gaacattga agaaattgc gattttctt 
      caacaagtt tacaaccct gccggagaa tttgttcaa 
      aaacaacgg caaagcgaa gcgggtggt tttgttttc 
      gaacaatat cactgattt gacgtggga cgagttcgc 
      agggctgga ctcagtgac cgatctttt gattgggcg 
      caattcagt cgcacattt cgacgctgg aaaaatagc 
      gaaacacat gccacaatt gagagagct taacatgga 
      gctaaaccc gatttcgtg cgaagaaat cgaacaatt 
      cgccaagga attgacgtc gaagttgaa ccacactgt 
      gaaagtcac acacatgtc cgatccctc agcccccaa 
      ttcttgtat ttaccaata aataattcc agtttttgc 
      gagtcgttg ttaattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY