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Gene information
General information
TC002658 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17160734..17162717
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002658
    iBeetle screen iB_07031

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03432

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head capsule potentially size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    gena triplet bristle number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002658 RA
      MPGRNFHSF WTIGKFDIL KLNGKVAAR CLVCDKILK 
      NTSKQRLMS HRNVCKEEN TSKEVAEVG EEDEIILVE 
      IESKADDND EEESNEVSV EDSPSCSNE QFNDRVSAA 
      STLTLPTSD LNYDENICN TESSNLTSI SIAASPSPR 
      MLSPIPSTS KESIHKIIN KSISKKSNS KPKDTIKNF 
      LDHVSNNQK IEINKALGK FIVGCNIPF HVVDSIHFK 
      DFLRVLRPA YQPPCRKTV SGTILNSLY DEINEDDQN 
      SIGKFSVMM IDGWKNSAN NTKQVVTIL KSTRSDKCI 
      FLEAYDVTT TPETGEKLS ELCNLSVEL AKTKFKTEI 
      YAVVSDNAA NMLKMGKLT SDLMHTTCN SHTGNLLAK 
      DLISKELCS KVITILKEF KHPNLEKEL QSQGGSKIK 
      LAVDTRWCT YRDSFSCLI KNLKFMKKL AAEETIKLK 
      QEIIQLLFD DEFIDEVKT TIEVSNPVC KLINECQSS 
      SCTIADACE KWTHLQLPA EFEDVLKRR RDMALNIYS 
      LTSNFLHPL YRGRSLNQT QVDMVHDYL VATLSAEGL 
      MSFHQFCSS EDIFETLER KNICCPKVF WGLAERKHP 
      ELASVALKL INIPASSAS LERVFSNWA FIHNDLRNR 
      LDADHSRKI MHCYYSLKM RESNEVS
    CDS sequence
    • >TC002658 RA
      atgccggga agaaatttt cacagtttt tggacgatt 
      ggaaagttt gatatatta aaactgaat ggaaaagtt 
      gcagcacgt tgtcttgtg tgtgataaa atattaaaa 
      aatacttcg aaacaacga ctgatgtcg catagaaat 
      gtctgcaag gaagaaaat acatccaaa gaagtggcc 
      gaagtgggt gaagaggat gaaatcata ttggtagaa 
      atagaaagt aaggctgac gataacgat gaagaagag 
      tcaaatgaa gtttcagtg gaagattca ccaagttgt 
      agcaatgaa caatttaat gatcgtgta agtgcggca 
      tcaacatta acattacct acatcagat ttaaactat 
      gatgaaaac atttgcaat actgagagt tcaaatttg 
      acttcaata tcaatagca gcttcacct tcaccccga 
      atgctatcc ccaattcca tcaacttca aaagaaagc 
      atacacaaa ataattaac aagtcaata tctaaaaaa 
      agcaacagc aagccaaag gatacaatt aaaaatttt 
      ttagaccat gttagcaac aatcagaaa atcgagatt 
      aacaaagca cttggcaaa tttattgta ggttgtaac 
      attccattt catgtagta gactcaatt catttcaaa 
      gactttttg agagtgctt agacctgca tatcagcct 
      ccctgccgc aagacagtg tcagggacc attctaaat 
      tcattatat gatgaaata aatgaagac gatcaaaac 
      tcaataggt aaattctct gttatgatg atcgatggg 
      tggaaaaat tcagcaaat aacacaaaa caagtggta 
      acaattttg aagtcaaca agaagcgat aagtgtatt 
      tttctggaa gcatatgat gtaacaaca acaccagag 
      acgggagaa aagctaagt gaattgtgt aatctatct 
      gttgaacta gcgaagaca aagtttaag actgaaatt 
      tatgctgtc gttagcgat aatgcagca aacatgttg 
      aaaatggga aaattgacg agtgattta atgcatact 
      acttgcaat agtcacaca ggaaatctt cttgccaaa 
      gacttaatt tccaaagaa ttatgttcc aaagtaatc 
      actatctta aaagaattt aaacatcct aatctcgaa 
      aaggagctg caaagtcag ggaggatca aagataaaa 
      ttagctgtc gatactcgg tggtgtacc tatagagac 
      tcattttct tgcttgatc aaaaattta aaatttatg 
      aaaaaactt gcggcagaa gaaactata aaacttaaa 
      caagaaata attcagtta ctatttgac gatgagttc 
      atcgatgaa gttaaaaca acaattgaa gtatctaac 
      ccggtttgc aaattaatt aatgagtgt cagtctagc 
      agctgcact attgccgat gcttgtgag aaatggact 
      catttacaa ctgcctgcg gagtttgaa gatgttttg 
      aaaagaaga agagacatg gcgctaaat atttactct 
      ttaacatca aattttctt catcctctt tatagagga 
      aggtctttg aatcaaacc caggttgat atggtccat 
      gactatttg gtagctact ttaagtgca gaaggttta 
      atgagtttt caccaattt tgttcttcg gaagatatt 
      tttgaaacc ttagaacgt aaaaacatt tgttgccca 
      aaagttttt tggggattg gccgagaga aagcaccca 
      gagttggct agtgtagct ttaaaactt atcaatata 
      ccagccagc tcagcttcc ttggagcgt gtgtttagt 
      aattgggca tttattcac aatgatttg agaaatcgt 
      ttggatgct gatcattcg agaaaaata atgcattgt 
      tattactcc ctgaagatg cgggaaagt aacgaagtc 
      agttaa
    mRNA sequence
    • >TC002658 RA
      atgccggga agaaatttt cacagtttt tggacgatt 
      ggaaagttt gatatatta aaactgaat ggaaaagtt 
      gcagcacgt tgtcttgtg tgtgataaa atattaaaa 
      aatacttcg aaacaacga ctgatgtcg catagaaat 
      gtctgcaag gaagaaaat acatccaaa gaagtggcc 
      gaagtgggt gaagaggat gaaatcata ttggtagaa 
      atagaaagt aaggctgac gataacgat gaagaagag 
      tcaaatgaa gtttcagtg gaagattca ccaagttgt 
      agcaatgaa caatttaat gatcgtgta agtgcggca 
      tcaacatta acattacct acatcagat ttaaactat 
      gatgaaaac atttgcaat actgagagt tcaaatttg 
      acttcaata tcaatagca gcttcacct tcaccccga 
      atgctatcc ccaattcca tcaacttca aaagaaagc 
      atacacaaa ataattaac aagtcaata tctaaaaaa 
      agcaacagc aagccaaag gatacaatt aaaaatttt 
      ttagaccat gttagcaac aatcagaaa atcgagatt 
      aacaaagca cttggcaaa tttattgta ggttgtaac 
      attccattt catgtagta gactcaatt catttcaaa 
      gactttttg agagtgctt agacctgca tatcagcct 
      ccctgccgc aagacagtg tcagggacc attctaaat 
      tcattatat gatgaaata aatgaagac gatcaaaac 
      tcaataggt aaattctct gttatgatg atcgatggg 
      tggaaaaat tcagcaaat aacacaaaa caagtggta 
      acaattttg aagtcaaca agaagcgat aagtgtatt 
      tttctggaa gcatatgat gtaacaaca acaccagag 
      acgggagaa aagctaagt gaattgtgt aatctatct 
      gttgaacta gcgaagaca aagtttaag actgaaatt 
      tatgctgtc gttagcgat aatgcagca aacatgttg 
      aaaatggga aaattgacg agtgattta atgcatact 
      acttgcaat agtcacaca ggaaatctt cttgccaaa 
      gacttaatt tccaaagaa ttatgttcc aaagtaatc 
      actatctta aaagaattt aaacatcct aatctcgaa 
      aaggagctg caaagtcag ggaggatca aagataaaa 
      ttagctgtc gatactcgg tggtgtacc tatagagac 
      tcattttct tgcttgatc aaaaattta aaatttatg 
      aaaaaactt gcggcagaa gaaactata aaacttaaa 
      caagaaata attcagtta ctatttgac gatgagttc 
      atcgatgaa gttaaaaca acaattgaa gtatctaac 
      ccggtttgc aaattaatt aatgagtgt cagtctagc 
      agctgcact attgccgat gcttgtgag aaatggact 
      catttacaa ctgcctgcg gagtttgaa gatgttttg 
      aaaagaaga agagacatg gcgctaaat atttactct 
      ttaacatca aattttctt catcctctt tatagagga 
      aggtctttg aatcaaacc caggttgat atggtccat 
      gactatttg gtagctact ttaagtgca gaaggttta 
      atgagtttt caccaattt tgttcttcg gaagatatt 
      tttgaaacc ttagaacgt aaaaacatt tgttgccca 
      aaagttttt tggggattg gccgagaga aagcaccca 
      gagttggct agtgtagct ttaaaactt atcaatata 
      ccagccagc tcagcttcc ttggagcgt gtgtttagt 
      aattgggca tttattcac aatgatttg agaaatcgt 
      ttggatgct gatcattcg agaaaaata atgcattgt 
      tattactcc ctgaagatg cgggaaagt aacgaagtc 
      agttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY