Status
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Gene information
General information
TC002638 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 16246585..16256148
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002638
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03428

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002638 RA
      MAELVQRHL ESMAEEIEE MRRTRLYTL EETRLILKN 
      RKEFEYKIN GVSKNLQDF MEYITYEKV LLKQIEHRR 
      DKNTIAERK SSIEYKILR RIKNLYEIA LQRFSGDFS 
      LCLSYFKFC KQTHDINAA NIALQTMIK NCSQTPEVW 
      QVAAAWHAH DRKDLNSAL IALNKGLTL HKNSQLLYT 
      EAIKLELSV IKNNAPEDT ELQRRVCAK IETYLQLIC 
      VHINDFKFL IEILNLLEL YSFTKSVQN LIIDKLLKN 
      HSESELVWD ALAQRERRG FHYGQSNSE QANSKEVCL 
      SLCFAKYEE GLKKVSPSE KASLWSVYL DCLEEARLE 
      NNQLPDILK TLKMALKQA SDENYLPDR YYIKWVELA 
      NDKEVLHIL EKGTSAFPE SIDLWKLLL RYATINDME 
      KKVNEIFDK GTQMLKENS LPLWFMYLR YYGLLAKDD 
      VIDSTYRKA LTQPPEISQ VLKPRYIEW LALQKGIND 
      ARKMYNVLA NQEPFCKEL HSTMSKLES MEIQHDYKV 
      WACVHQLAC KQFGREDVD VWINHILFY LHFNKTDSV 
      NENVQRIYR DAENTLTPL LLPDFKERY EKALSEL
    CDS sequence
    • >TC002638 RA
      atggcagag ctcgtgcag cgtcatttg gagtctatg 
      gctgaagaa atagaagaa atgaggaga accagatta 
      tacactctt gaagaaacc agattaata ttaaagaat 
      cgaaaagag tttgagtac aaaatcaac ggcgtttcg 
      aaaaactta caggatttt atggagtac attacgtac 
      gagaaagtg ttattaaag cagattgag catcgacgg 
      gacaaaaac acgattgct gaacgaaaa agtagtatc 
      gaatataaa attttgcga agaataaaa aatttgtac 
      gagatcgca ttacaaaga ttttctggt gacttttct 
      ttgtgttta tcatatttt aaattttgt aagcagaca 
      catgacatc aatgccgct aatattgca ctacaaaca 
      atgatcaag aattgttcc caaacccca gaagtgtgg 
      caagtggcc gctgcatgg cacgcccac gaccgcaaa 
      gacctcaac tctgctttg atcgcctta aataaaggc 
      ttgactctt cacaaaaac agtcagttg ttgtataca 
      gaagccata aagttggaa ctctctgtt atcaagaat 
      aacgcaccg gaagataca gaactgcaa agaagggtg 
      tgtgcaaaa atagaaacg tacctccag cttatttgt 
      gtccatata aacgacttt aaattttta atcgaaatt 
      ttaaatctg ctagaatta tacagcttt acaaaaagc 
      gttcaaaat ttaataata gacaaactt ttgaaaaat 
      cactcggag tcggagctt gtttgggat gctttagca 
      cagcgagaa agaagagga tttcactat ggtcaatct 
      aatagcgaa caagcaaat tccaaagaa gtttgttta 
      agtttgtgt ttcgctaaa tatgaggaa ggtttgaag 
      aaagtttca ccctctgaa aaagccagt ttatggtct 
      gtttattta gattgctta gaggaagca agactggaa 
      aataaccaa ctaccagat atcctcaaa acattgaaa 
      atggcactt aaacaagcc tcagatgag aattattta 
      cccgatagg tattacatc aagtgggtg gaactggcc 
      aatgataaa gaggtccta cacatactc gaaaaagga 
      acttcagca tttcccgaa tccatcgat ttatggaaa 
      ttgctgctt cgctacgcg acgatcaac gatatggaa 
      aagaaagtc aacgaaatt ttcgataaa ggaacgcag 
      atgttaaaa gagaattct ttgcctttg tggtttatg 
      tatttgcgt tactacggt ttgttggcg aaagatgac 
      gtcatagac agtacatac cgtaaagct ctaacgcag 
      ccacctgaa atttctcaa gtgcttaaa ccgagatat 
      attgaatgg ttggctctt cagaaaggt ataaatgat 
      gcgcgaaaa atgtataat gtgctagca aatcaagaa 
      ccgttctgc aaagagtta cactctaca atgtccaaa 
      ttggagtca atggaaatc caacatgat tataaagta 
      tgggcttgc gttcatcaa ttggcctgc aagcaattt 
      gggcgagag gatgtcgat gtgtggatt aatcatatt 
      ttgttttat ttgcatttt aataaaacg gacagtgtt 
      aatgagaac gtgcaacga atttatagg gatgcggaa 
      aatactctt actcccttg ttgctaccg gattttaaa 
      gagagatat gaaaaagcg ctttctgaa ttgtaa
    mRNA sequence
    • >TC002638 RA
      tttggagtg gttttattt aatttttga attattttg 
      aaaaaaaca caatggcag agctcgtgc agcgtcatt 
      tggagtcta tggctgaag aaatagaag aaatgagga 
      gaaccagat tatacactc ttgaagaaa ccagattaa 
      tattaaaga atcgaaaag agtttgagt acaaaatca 
      acggcgttt cgaaaaact tacaggatt ttatggagt 
      acattacgt acgagaaag tgttattaa agcagattg 
      agcatcgac gggacaaaa acacgattg ctgaacgaa 
      aaagtagta tcgaatata aaattttgc gaagaataa 
      aaaatttgt acgagatcg cattacaaa gattttctg 
      gtgactttt ctttgtgtt tatcatatt ttaaatttt 
      gtaagcaga cacatgaca tcaatgccg ctaatattg 
      cactacaaa caatgatca agaattgtt cccaaaccc 
      cagaagtgt ggcaagtgg ccgctgcat ggcacgccc 
      acgaccgca aagacctca actctgctt tgatcgcct 
      taaataaag gcttgactc ttcacaaaa acagtcagt 
      tgttgtata cagaagcca taaagttgg aactctctg 
      ttatcaaga ataacgcac cggaagata cagaactgc 
      aaagaaggg tgtgtgcaa aaatagaaa cgtacctcc 
      agcttattt gtgtccata taaacgact ttaaatttt 
      taatcgaaa ttttaaatc tgctagaat tatacagct 
      ttacaaaaa gcgttcaaa atttaataa tagacaaac 
      ttttgaaaa atcactcgg agtcggagc ttgtttggg 
      atgctttag cacagcgag aaagaagag gatttcact 
      atggtcaat ctaatagcg aacaagcaa attccaaag 
      aagtttgtt taagtttgt gtttcgcta aatatgagg 
      aaggtttga agaaagttt caccctctg aaaaagcca 
      gtttatggt ctgtttatt tagattgct tagaggaag 
      caagactgg aaaataacc aactaccag atatcctca 
      aaacattga aaatggcac ttaaacaag cctcagatg 
      agaattatt tacccgata ggtattaca tcaagtggg 
      tggaactgg ccaatgata aagaggtcc tacacatac 
      tcgaaaaag gaacttcag catttcccg aatccatcg 
      atttatgga aattgctgc ttcgctacg cgacgatca 
      acgatatgg aaaagaaag tcaacgaaa ttttcgata 
      aaggaacgc agatgttaa aagagaatt ctttgcctt 
      tgtggttta tgtatttgc gttactacg gtttgttgg 
      cgaaagatg acgtcatag acagtacat accgtaaag 
      ctctaacgc agccacctg aaatttctc aagtgctta 
      aaccgagat atattgaat ggttggctc ttcagaaag 
      gtataaatg atgcgcgaa aaatgtata atgtgctag 
      caaatcaag aaccgttct gcaaagagt tacactcta 
      caatgtcca aattggagt caatggaaa tccaacatg 
      attataaag tatgggctt gcgttcatc aattggcct 
      gcaagcaat ttgggcgag aggatgtcg atgtgtgga 
      ttaatcata ttttgtttt atttgcatt ttaataaaa 
      cggacagtg ttaatgaga acgtgcaac gaatttata 
      gggatgcgg aaaatactc ttactccct tgttgctac 
      cggatttta aagagagat atgaaaaag cgctttctg 
      aattgtaat tgtacattt tcagtcatg cagtgaatg 
      tgtgtattt tttcaataa acagttata aatgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY