| General information | |||
| TC002628 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 20969371..20981925 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002628 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03424 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC002628 RA
MDPLAGGEV NIGREWPTI NAKDDEKEE EASTFVDKK ILRMMKMMG YQSGFGLGK NKQGVTEPV AVEPHLSKR GLGLEIKNL DYSKEEWDY SLDKPTVVE KVSWLCSEK VLELSEDEM VKWPREGKP TNNMEEEFN FCDKQTLHD VLQGKNIFD VLDLKELCQ ARARANPFE TIRSVFFMN RASLKMANI DAATDFMFT NINHSHHED SGPYYFADV CAGPGGFTE YILWRKKWM FKGFGLTLR GDNDFKLTE SLCASPVTF LPLYGASGD GNACSPENI SDFSEKVLF ETENKGVHF MMADGGFSV EGNENIQEI LSKSLYICQ CLIALEILR KHGHFVTKV FDIFTCFSV GLLYLIYHC FEKVCILKP NSSRPANSE RYVICCNYK GRENVESIR IYLKKIVQR LWELKNDNN LDVIEIVPL SILKSDHNF YSYVCDSNN RIAKNQTIS LQKLAAFCR NPLLTEYRQ EELRRQCLD YWKIPDKPK IPLPKYTTE NLLDETVEN RELLLVQPR EIYSIEQLQ TVVDDLNDW HYALMQCKR ATNICNFYA GVGLSRVYR MQGNKWVRV KNLQLVRGT LLYGELVKE KCYMEESVG KVGYKYSLH VIDALRLGD LYLTDLKFD ERIKLIKIY CKAINYEFR TNSIRIRPK SIHDLQSLS LDLPLTKDQ SSGTYFSPL PVLGYKVNN EGFEVNSLL LLKTGQHQS FHSTYVLRV QMFLEGDQH FSLDSLVEL IRQKRALL
- >TC002628 RA
atggaccca cttgcaggg ggcgaagtg aacattggt cgagaatgg cccactata aatgcgaag gatgatgaa aaggaagag gaagcttca acatttgtt gataagaaa atcctcaga atgatgaaa atgatgggg tatcagtct ggttttggc ttgggtaag aacaagcaa ggtgtgacg gaacctgta gccgttgag ccacatttg agtaagcga ggtttagga ttggaaata aaaaatttg gattattct aaagaagag tgggactac tctttagat aaaccaaca gttgttgaa aaagttagc tggttgtgt agcgaaaaa gttttggaa ttgagcgag gatgagatg gttaaatgg ccaagggag ggcaagccc actaataat atggaagaa gagtttaat ttttgcgac aaacaaaca ttgcatgat gttctacag ggaaaaaat atatttgac gtactagac cttaaggaa ctctgccaa gctcgtgcg cgagcgaac ccttttgaa acaatccgt tccgttttt ttcatgaat cgagcgtcg ttaaaaatg gcaaacatt gacgctgca accgatttc atgttcaca aacataaat cacagccac catgaagat tccggacct tactatttt gcggacgtt tgtgccggg cctggggga tttaccgaa tacatttta tggcgaaaa aaatggatg tttaaggga tttggtctc acattacgt ggtgataac gattttaaa ttaacggag tcactttgt gcctctcct gtcactttt ttgccactg tacggagcc agcggtgac ggcaatgct tgctctcct gaaaatatc agcgatttt tctgagaag gttttgttc gaaacggag aacaaagga gtgcatttc atgatggca gatggcggg ttttccgtt gaagggaac gaaaatatc caagaaatt ctgtccaaa agtttgtac atttgtcag tgtttgatt gctttggaa attttgcgt aaacatggt cattttgtg accaaagta ttcgacatt tttacttgt tttagtgtt ggacttttg tacctaatt tatcattgt tttgagaaa gtatgcatt ttgaaacct aattcgtca aggcctgca aattcggaa cgatacgtc atttgttgt aattataag ggccgggaa aatgttgaa agcataaga atttatttg aaaaaaatt gttcaaaga ttgtgggaa ctgaaaaac gacaataat ttggacgta atagaaatc gtaccttta tctatccta aaaagcgat cacaatttc tattcgtac gtttgtgat agtaataat agaatagcc aaaaaccaa acgataagt ctacaaaaa cttgccgct ttttgtaga aaccctttg ttgacagag taccgacaa gaagaactg agaagacag tgtctagat tattggaaa attccagac aaacctaaa atacctctc cccaagtac acaacggag aacctactt gacgaaaca gtcgaaaat agagaatta cttttggtg caaccacgt gagatatac tcaattgaa caattacaa acggttgtg gatgactta aatgactgg cattacgct ctaatgcaa tgtaaaaga gccacaaac atttgcaac ttttatgca ggggtggga ttatcgcga gtctatcga atgcagggt aataaatgg gtgagagtg aaaaatttg caactggtt cgagggacc ttactgtat ggagaattg gtaaaggaa aaatgttac atggaagaa tcagttgga aaagttgga tataagtat agtttacac gtaatagac gcattgcga ttgggagac ctctacttg actgatcta aaatttgac gaaagaatt aaactaatt aaaatctat tgcaaagca attaattat gaatttcga acgaatagt atcaggatt cgtccaaaa tcaatacat gacttacag tccttatct ttggatctt cctttaact aaggatcag agttctgga acatatttt tcaccattg ccagttttg ggttataaa gtcaataat gaaggtttt gaagtgaat tcgcttcta cttttaaaa actggccaa caccaatcg tttcattct acgtatgtt ctaagggtg caaatgttt ttggaaggg gaccaacat ttttccttg gatagttta gtagagtta attcggcaa aaaagggct ttgttgtga
- >TC002628 RA
acgcacaac gtgaagtgg gttgcactt ctaacctca ctttctgac tattgttta ttttcgagt actgaaata tctctaata agggtattt atgttttac gctaagcag tagtgaact acaatttgg agcattttt ctattgtaa aaatggacc cacttgcag ggggcgaag tgaacattg gtcgagaat ggcccacta taaatgcga aggatgatg aaaaggaag aggaagctt caacatttg ttgataaga aaatcctca gaatgatga aaatgatgg ggtatcagt ctggttttg gcttgggta agaacaagc aaggtgtga cggaacctg tagccgttg agccacatt tgagtaagc gaggtttag gattggaaa taaaaaatt tggattatt ctaaagaag agtgggact actctttag ataaaccaa cagttgttg aaaaagtta gctggttgt gtagcgaaa aagttttgg aattgagcg aggatgaga tggttaaat ggccaaggg agggcaagc ccactaata atatggaag aagagttta atttttgcg acaaacaaa cattgcatg atgttctac agggaaaaa atatatttg acgtactag accttaagg aactctgcc aagctcgtg cgcgagcga acccttttg aaacaatcc gttccgttt ttttcatga atcgagcgt cgttaaaaa tggcaaaca ttgacgctg caaccgatt tcatgttca caaacataa atcacagcc accatgaag attccggac cttactatt ttgcggacg tttgtgccg ggcctgggg gatttaccg aatacattt tatggcgaa aaaaatgga tgtttaagg gatttggtc tcacattac gtggtgata acgatttta aattaacgg agtcacttt gtgcctctc ctgtcactt ttttgccac tgtacggag ccagcggtg acggcaatg cttgctctc ctgaaaata tcagcgatt tttctgaga aggttttgt tcgaaacgg agaacaaag gagtgcatt tcatgatgg cagatggcg ggttttccg ttgaaggga acgaaaata tccaagaaa ttctgtcca aaagtttgt acatttgtc agtgtttga ttgctttgg aaattttgc gtaaacatg gtcattttg tgaccaaag tattcgaca tttttactt gttttagtg ttggacttt tgtacctaa tttatcatt gttttgaga aagtatgca ttttgaaac ctaattcgt caaggcctg caaattcgg aacgatacg tcatttgtt gtaattata agggccggg aaaatgttg aaagcataa gaatttatt tgaaaaaaa ttgttcaaa gattgtggg aactgaaaa acgacaata atttggacg taatagaaa tcgtacctt tatctatcc taaaaagcg atcacaatt tctattcgt acgtttgtg atagtaata atagaatag ccaaaaacc aaacgataa gtctacaaa aacttgccg ctttttgta gaaaccctt tgttgacag agtaccgac aagaagaac tgagaagac agtgtctag attattgga aaattccag acaaaccta aaatacctc tccccaagt acacaacgg agaacctac ttgacgaaa cagtcgaaa atagagaat tacttttgg tgcaaccac gtgagatat actcaattg aacaattac aaacggttg tggatgact taaatgact ggcattacg ctctaatgc aatgtaaaa gagccacaa acatttgca acttttatg caggggtgg gattatcgc gagtctatc gaatgcagg gtaataaat gggtgagag tgaaaaatt tgcaactgg ttcgaggga ccttactgt atggagaat tggtaaagg aaaaatgtt acatggaag aatcagttg gaaaagttg gatataagt atagtttac acgtaatag acgcattgc gattgggag acctctact tgactgatc taaaatttg acgaaagaa ttaaactaa ttaaaatct attgcaaag caattaatt atgaatttc gaacgaata gtatcagga ttcgtccaa aatcaatac atgacttac agtccttat ctttggatc ttcctttaa ctaaggatc agagttctg gaacatatt tttcaccat tgccagttt tgggttata aagtcaata atgaaggtt ttgaagtga attcgcttc tacttttaa aaactggcc aacaccaat cgtttcatt ctacgtatg ttctaaggg tgcaaatgt ttttggaag gggaccaac atttttcct tggatagtt tagtagagt taattcggc aaaaaaggg ctttgttgt gacatgtgt gttgtgttc gttgttatt aagtaaata attcacttg cagtaagga atgcattaa ctagattct caacgacgt taaaagatc acaatattt tttttccta cctcatatt atccatttt tgagttaac agtatttct tagattaat taagttgtt ttaacactt gtactttta ggtcacaaa cactgtaaa tagttttag aatccatat tcgttgact cgttgtttt tcatgttac tgataaaaa atatatttt tcttaaaaa a
