Status
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Gene information
General information
TC002628 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 20969371..20981925
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002628
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03424

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002628 RA
      MDPLAGGEV NIGREWPTI NAKDDEKEE EASTFVDKK 
      ILRMMKMMG YQSGFGLGK NKQGVTEPV AVEPHLSKR 
      GLGLEIKNL DYSKEEWDY SLDKPTVVE KVSWLCSEK 
      VLELSEDEM VKWPREGKP TNNMEEEFN FCDKQTLHD 
      VLQGKNIFD VLDLKELCQ ARARANPFE TIRSVFFMN 
      RASLKMANI DAATDFMFT NINHSHHED SGPYYFADV 
      CAGPGGFTE YILWRKKWM FKGFGLTLR GDNDFKLTE 
      SLCASPVTF LPLYGASGD GNACSPENI SDFSEKVLF 
      ETENKGVHF MMADGGFSV EGNENIQEI LSKSLYICQ 
      CLIALEILR KHGHFVTKV FDIFTCFSV GLLYLIYHC 
      FEKVCILKP NSSRPANSE RYVICCNYK GRENVESIR 
      IYLKKIVQR LWELKNDNN LDVIEIVPL SILKSDHNF 
      YSYVCDSNN RIAKNQTIS LQKLAAFCR NPLLTEYRQ 
      EELRRQCLD YWKIPDKPK IPLPKYTTE NLLDETVEN 
      RELLLVQPR EIYSIEQLQ TVVDDLNDW HYALMQCKR 
      ATNICNFYA GVGLSRVYR MQGNKWVRV KNLQLVRGT 
      LLYGELVKE KCYMEESVG KVGYKYSLH VIDALRLGD 
      LYLTDLKFD ERIKLIKIY CKAINYEFR TNSIRIRPK 
      SIHDLQSLS LDLPLTKDQ SSGTYFSPL PVLGYKVNN 
      EGFEVNSLL LLKTGQHQS FHSTYVLRV QMFLEGDQH 
      FSLDSLVEL IRQKRALL
    CDS sequence
    • >TC002628 RA
      atggaccca cttgcaggg ggcgaagtg aacattggt 
      cgagaatgg cccactata aatgcgaag gatgatgaa 
      aaggaagag gaagcttca acatttgtt gataagaaa 
      atcctcaga atgatgaaa atgatgggg tatcagtct 
      ggttttggc ttgggtaag aacaagcaa ggtgtgacg 
      gaacctgta gccgttgag ccacatttg agtaagcga 
      ggtttagga ttggaaata aaaaatttg gattattct 
      aaagaagag tgggactac tctttagat aaaccaaca 
      gttgttgaa aaagttagc tggttgtgt agcgaaaaa 
      gttttggaa ttgagcgag gatgagatg gttaaatgg 
      ccaagggag ggcaagccc actaataat atggaagaa 
      gagtttaat ttttgcgac aaacaaaca ttgcatgat 
      gttctacag ggaaaaaat atatttgac gtactagac 
      cttaaggaa ctctgccaa gctcgtgcg cgagcgaac 
      ccttttgaa acaatccgt tccgttttt ttcatgaat 
      cgagcgtcg ttaaaaatg gcaaacatt gacgctgca 
      accgatttc atgttcaca aacataaat cacagccac 
      catgaagat tccggacct tactatttt gcggacgtt 
      tgtgccggg cctggggga tttaccgaa tacatttta 
      tggcgaaaa aaatggatg tttaaggga tttggtctc 
      acattacgt ggtgataac gattttaaa ttaacggag 
      tcactttgt gcctctcct gtcactttt ttgccactg 
      tacggagcc agcggtgac ggcaatgct tgctctcct 
      gaaaatatc agcgatttt tctgagaag gttttgttc 
      gaaacggag aacaaagga gtgcatttc atgatggca 
      gatggcggg ttttccgtt gaagggaac gaaaatatc 
      caagaaatt ctgtccaaa agtttgtac atttgtcag 
      tgtttgatt gctttggaa attttgcgt aaacatggt 
      cattttgtg accaaagta ttcgacatt tttacttgt 
      tttagtgtt ggacttttg tacctaatt tatcattgt 
      tttgagaaa gtatgcatt ttgaaacct aattcgtca 
      aggcctgca aattcggaa cgatacgtc atttgttgt 
      aattataag ggccgggaa aatgttgaa agcataaga 
      atttatttg aaaaaaatt gttcaaaga ttgtgggaa 
      ctgaaaaac gacaataat ttggacgta atagaaatc 
      gtaccttta tctatccta aaaagcgat cacaatttc 
      tattcgtac gtttgtgat agtaataat agaatagcc 
      aaaaaccaa acgataagt ctacaaaaa cttgccgct 
      ttttgtaga aaccctttg ttgacagag taccgacaa 
      gaagaactg agaagacag tgtctagat tattggaaa 
      attccagac aaacctaaa atacctctc cccaagtac 
      acaacggag aacctactt gacgaaaca gtcgaaaat 
      agagaatta cttttggtg caaccacgt gagatatac 
      tcaattgaa caattacaa acggttgtg gatgactta 
      aatgactgg cattacgct ctaatgcaa tgtaaaaga 
      gccacaaac atttgcaac ttttatgca ggggtggga 
      ttatcgcga gtctatcga atgcagggt aataaatgg 
      gtgagagtg aaaaatttg caactggtt cgagggacc 
      ttactgtat ggagaattg gtaaaggaa aaatgttac 
      atggaagaa tcagttgga aaagttgga tataagtat 
      agtttacac gtaatagac gcattgcga ttgggagac 
      ctctacttg actgatcta aaatttgac gaaagaatt 
      aaactaatt aaaatctat tgcaaagca attaattat 
      gaatttcga acgaatagt atcaggatt cgtccaaaa 
      tcaatacat gacttacag tccttatct ttggatctt 
      cctttaact aaggatcag agttctgga acatatttt 
      tcaccattg ccagttttg ggttataaa gtcaataat 
      gaaggtttt gaagtgaat tcgcttcta cttttaaaa 
      actggccaa caccaatcg tttcattct acgtatgtt 
      ctaagggtg caaatgttt ttggaaggg gaccaacat 
      ttttccttg gatagttta gtagagtta attcggcaa 
      aaaagggct ttgttgtga
    mRNA sequence
    • >TC002628 RA
      acgcacaac gtgaagtgg gttgcactt ctaacctca 
      ctttctgac tattgttta ttttcgagt actgaaata 
      tctctaata agggtattt atgttttac gctaagcag 
      tagtgaact acaatttgg agcattttt ctattgtaa 
      aaatggacc cacttgcag ggggcgaag tgaacattg 
      gtcgagaat ggcccacta taaatgcga aggatgatg 
      aaaaggaag aggaagctt caacatttg ttgataaga 
      aaatcctca gaatgatga aaatgatgg ggtatcagt 
      ctggttttg gcttgggta agaacaagc aaggtgtga 
      cggaacctg tagccgttg agccacatt tgagtaagc 
      gaggtttag gattggaaa taaaaaatt tggattatt 
      ctaaagaag agtgggact actctttag ataaaccaa 
      cagttgttg aaaaagtta gctggttgt gtagcgaaa 
      aagttttgg aattgagcg aggatgaga tggttaaat 
      ggccaaggg agggcaagc ccactaata atatggaag 
      aagagttta atttttgcg acaaacaaa cattgcatg 
      atgttctac agggaaaaa atatatttg acgtactag 
      accttaagg aactctgcc aagctcgtg cgcgagcga 
      acccttttg aaacaatcc gttccgttt ttttcatga 
      atcgagcgt cgttaaaaa tggcaaaca ttgacgctg 
      caaccgatt tcatgttca caaacataa atcacagcc 
      accatgaag attccggac cttactatt ttgcggacg 
      tttgtgccg ggcctgggg gatttaccg aatacattt 
      tatggcgaa aaaaatgga tgtttaagg gatttggtc 
      tcacattac gtggtgata acgatttta aattaacgg 
      agtcacttt gtgcctctc ctgtcactt ttttgccac 
      tgtacggag ccagcggtg acggcaatg cttgctctc 
      ctgaaaata tcagcgatt tttctgaga aggttttgt 
      tcgaaacgg agaacaaag gagtgcatt tcatgatgg 
      cagatggcg ggttttccg ttgaaggga acgaaaata 
      tccaagaaa ttctgtcca aaagtttgt acatttgtc 
      agtgtttga ttgctttgg aaattttgc gtaaacatg 
      gtcattttg tgaccaaag tattcgaca tttttactt 
      gttttagtg ttggacttt tgtacctaa tttatcatt 
      gttttgaga aagtatgca ttttgaaac ctaattcgt 
      caaggcctg caaattcgg aacgatacg tcatttgtt 
      gtaattata agggccggg aaaatgttg aaagcataa 
      gaatttatt tgaaaaaaa ttgttcaaa gattgtggg 
      aactgaaaa acgacaata atttggacg taatagaaa 
      tcgtacctt tatctatcc taaaaagcg atcacaatt 
      tctattcgt acgtttgtg atagtaata atagaatag 
      ccaaaaacc aaacgataa gtctacaaa aacttgccg 
      ctttttgta gaaaccctt tgttgacag agtaccgac 
      aagaagaac tgagaagac agtgtctag attattgga 
      aaattccag acaaaccta aaatacctc tccccaagt 
      acacaacgg agaacctac ttgacgaaa cagtcgaaa 
      atagagaat tacttttgg tgcaaccac gtgagatat 
      actcaattg aacaattac aaacggttg tggatgact 
      taaatgact ggcattacg ctctaatgc aatgtaaaa 
      gagccacaa acatttgca acttttatg caggggtgg 
      gattatcgc gagtctatc gaatgcagg gtaataaat 
      gggtgagag tgaaaaatt tgcaactgg ttcgaggga 
      ccttactgt atggagaat tggtaaagg aaaaatgtt 
      acatggaag aatcagttg gaaaagttg gatataagt 
      atagtttac acgtaatag acgcattgc gattgggag 
      acctctact tgactgatc taaaatttg acgaaagaa 
      ttaaactaa ttaaaatct attgcaaag caattaatt 
      atgaatttc gaacgaata gtatcagga ttcgtccaa 
      aatcaatac atgacttac agtccttat ctttggatc 
      ttcctttaa ctaaggatc agagttctg gaacatatt 
      tttcaccat tgccagttt tgggttata aagtcaata 
      atgaaggtt ttgaagtga attcgcttc tacttttaa 
      aaactggcc aacaccaat cgtttcatt ctacgtatg 
      ttctaaggg tgcaaatgt ttttggaag gggaccaac 
      atttttcct tggatagtt tagtagagt taattcggc 
      aaaaaaggg ctttgttgt gacatgtgt gttgtgttc 
      gttgttatt aagtaaata attcacttg cagtaagga 
      atgcattaa ctagattct caacgacgt taaaagatc 
      acaatattt tttttccta cctcatatt atccatttt 
      tgagttaac agtatttct tagattaat taagttgtt 
      ttaacactt gtactttta ggtcacaaa cactgtaaa 
      tagttttag aatccatat tcgttgact cgttgtttt 
      tcatgttac tgataaaaa atatatttt tcttaaaaa 
      a
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