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Gene information
General information
TC002622 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 21109330..21116471
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002622
    iBeetle screen iB_03423

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002622 RA
      MLFTKGLAF KTRLLFSQT ARFLNSNLM PNFSQFSIE 
      PKFNDAEQE KILNILNES NKDQLQCLN VAQTNIKRL 
      ENYKKRNGP FKSLNELLE VDGLSVKML EKMCKQLIS 
      DSTNSNVKT TNENKKMRQ ILTPQLPAS THVSTAVGI 
      HLAPMGVSW AKLSGNQLE SWGYEDFTN LPKKMFSTD 
      TFKLALGIL NQIPTGDAY IFEGTSSMG LQGQKQSSA 
      VPPYTQQLE LCSMLLALI NTSSSHNTV SQDADSDAD 
      ITNCVYFLR SKLPARMFK TLVGNECVS CTSVILHLL 
      GDSPDGVML PCSSITFEE KLKSDYLGK SPAHKELLG 
      QALMLVIAF MDLCVRKNP VSIKAIAPN RK
    CDS sequence
    • >TC002622 RA
      atgttattt acaaaagga ctggccttc aagactcgc 
      ttgttgttt tcacaaacg gcaagattt ttaaattcc 
      aatttaatg ccaaacttc tcccaattt agtattgaa 
      ccaaaattt aatgacgcc gaacaagaa aaaatttta 
      aatatcctc aacgaatct aataaagat caactacaa 
      tgtttgaac gttgcacaa actaatata aaaagatta 
      gaaaattac aaaaagaga aacggaccg tttaagtca 
      ctcaatgaa ttgttggaa gtggacggc ttgagtgtt 
      aaaatgctg gaaaaaatg tgcaaacaa ttaatttca 
      gattccact aattcaaac gtgaagaca actaatgag 
      aataaaaaa atgaggcaa attttaaca ccccaacta 
      ccggcgtcc actcatgtg tcaacggca gtaggtata 
      catttagca ccaatgggt gtaagttgg gctaaatta 
      tcaggaaat cagcttgag tcttggggc tatgaagac 
      tttacgaat cttcctaaa aagatgttt tctactgac 
      acttttaaa ctggctttg ggtattttg aaccaaatc 
      ccaacaggc gatgcttac atttttgaa ggaacttca 
      agcatgggg ttacagggt caaaaacaa tcctcagca 
      gtgcctcct tacactcaa caattagag ttgtgctcc 
      atgttattg gcacttatc aacaccagc tccagtcat 
      aacacagtt tcacaagat gctgattcg gatgcagat 
      ataacaaat tgtgtatat tttttaagg tcaaagttg 
      cctgcccga atgtttaaa actttggtt ggcaatgaa 
      tgtgtttct tgcacctcg gtaatactc catttattg 
      ggtgattct cccgatggt gtgatgcta ccttgttct 
      tcgattact tttgaagaa aagttgaag agtgattat 
      ttggggaag tcgccagct cacaaagag cttctaggc 
      caggctcta atgctagtg attgctttt atggatctt 
      tgtgttcga aaaaaccca gttagtatc aaagctatt 
      gcgcctaat agaaaatga
    mRNA sequence
    • >TC002622 RA
      ggttttttt tcaattact ttctagact ctactcaat 
      agtatatta aataacaag tcaataaag tggagcttt 
      acacgacga ggtgattgt tacgaggcc gacaggccg 
      agtatttta ataacctca atgtactca tttaagaac 
      aagtccgca ataggttat gtttttcaa taattatgt 
      catagcatg ttatgtttg tgagtgaat aaaattcca 
      aaatttcgt tcttttcga catgattta atttatttt 
      cacattgaa caaataaaa cagaaaatt tctaacggg 
      tgacaacat ttccaacaa tgttattta caaaaggac 
      tggccttca agactcgct tgttgtttt cacaaacgg 
      caagatttt taaattcca atttaatgc caaacttct 
      cccaattta gtattgaac caaaattta atgacgccg 
      aacaagaaa aaattttaa atatcctca acgaatcta 
      ataaagatc aactacaat gtttgaacg ttgcacaaa 
      ctaatataa aaagattag aaaattaca aaaagagaa 
      acggaccgt ttaagtcac tcaatgaat tgttggaag 
      tggacggct tgagtgtta aaatgctgg aaaaaatgt 
      gcaaacaat taatttcag attccacta attcaaacg 
      tgaagacaa ctaatgaga ataaaaaaa tgaggcaaa 
      ttttaacac cccaactac cggcgtcca ctcatgtgt 
      caacggcag taggtatac atttagcac caatgggtg 
      taagttggg ctaaattat caggaaatc agcttgagt 
      cttggggct atgaagact ttacgaatc ttcctaaaa 
      agatgtttt ctactgaca cttttaaac tggctttgg 
      gtattttga accaaatcc caacaggcg atgcttaca 
      tttttgaag gaacttcaa gcatggggt tacagggtc 
      aaaaacaat cctcagcag tgcctcctt acactcaac 
      aattagagt tgtgctcca tgttattgg cacttatca 
      acaccagct ccagtcata acacagttt cacaagatg 
      ctgattcgg atgcagata taacaaatt gtgtatatt 
      ttttaaggt caaagttgc ctgcccgaa tgtttaaaa 
      ctttggttg gcaatgaat gtgtttctt gcacctcgg 
      taatactcc atttattgg gtgattctc ccgatggtg 
      tgatgctac cttgttctt cgattactt ttgaagaaa 
      agttgaaga gtgattatt tggggaagt cgccagctc 
      acaaagagc ttctaggcc aggctctaa tgctagtga 
      ttgctttta tggatcttt gtgttcgaa aaaacccag 
      ttagtatca aagctattg cgcctaata gaaaatgaa 
      gtgtaaatt atgtaaata aataaaaaa attagaaaa 
      aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY