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Gene information
General information
TC002621 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid22: 1502322..1517720
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002621
    iBeetle screen iB_03422
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Pupal morphology
    head orientation irregular - Number of animals showing the phenotype: 10

    wings distal bent - Number of animals showing the phenotype: 10
    longitudinal dorsal thoracic muscles fluorescence decreased - Number of animals showing the phenotype: 10

    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 7

    elytra distal bent - Number of animals showing the phenotype: 4


    motility decreased - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    ovary without detectable defects - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002621 RA
      MNPTLWPQD VPFPPDEGK EEVASTYGD GDTNSQNDR 
      SGCFSPNGV SGNLQYPAY FKDAKGMNH SVFGSSTNF 
      GALQYLKQQ QAGKVLPDV IQHGRSAGM PLYVRCPIC 
      QKEFKQKST LLQHGCIHI ESRPYPCPE CGKRFRQQS 
      HLTQHLRIH TNEKPYGCV YCGRNFRQR TILNQHLRI 
      HTGEKPYKC GQCGKDFRQ KAILDQHTR THQGDRPFC 
      CPMPNCRRR FATEPEVKK HIDNHMNPH AAKTRRDST 
      GSDGKLTAN GMLPRGLTP TTVVKPELY FPQCYAPSF 
      NPPTSVAQF QAANPEFKP PNALPTQ
    CDS sequence
    • >TC002621 RA
      atgaacccc acactctgg ccgcaagac gtgccgttc 
      ccgcccgac gagggcaag gaggaggtg gcgtcgacg 
      tacggcgac ggagacacc aactcgcaa aacgaccgc 
      tccggctgt ttctccccc aacggcgtt tccggcaac 
      ttgcagtac cccgcgtac tttaaagac gccaaaggc 
      atgaaccac tctgtgttc gggtcgagt acgaacttt 
      ggtgcgttg caatacctc aagcagcag caagcgggc 
      aaagtcctg cccgatgtc atccagcac gggcgctcg 
      gcggggatg cccctctac gtgcggtgt cccatctgc 
      cagaaggag ttcaagcag aagtcgacg ctgctgcag 
      cacgggtgt atacatatc gagtcgagg ccgtatccg 
      tgcccggaa tgcgggaag aggttccga cagcagtcg 
      catctcacg cagcacttg aggattcat acgaacgaa 
      aaaccctac gggtgcgtc tactgcggc aggaatttc 
      agacagagg accattttg aatcagcat ttgaggata 
      cacaccggg gagaagccg tacaagtgt ggacagtgc 
      gggaaggac tttcggcag aaggcgatc ctggatcag 
      cacacgagg acgcatcaa ggtgaccga ccattctgt 
      tgcccgatg cccaactgc cggcgccgc ttcgccacc 
      gagcccgag gtcaagaag cacatcgac aaccacatg 
      aacccgcat gctgcgaag acgcgccgc gactccacc 
      ggctccgac ggcaagctg acagccaat gggatgctg 
      ccacgcggc ctcaccccc accaccgtg gtcaagccc 
      gagctgtac ttcccccag tgttacgcg cccagcttc 
      aaccctcca accagcgta gcccagttc caggctgcg 
      aacccggaa ttcaagccg cccaacgcg ctgccgacc 
      cagtga
    mRNA sequence
    • >TC002621 RA
      tgcaccaca gtggacaca aaggaatga aactgtttt 
      tgtttctga gacgtgagc cctcacctg gtgttcaag 
      aacggcatg aaccccaca ctctggccg caagacgtg 
      ccgttcccg cccgacgag ggcaaggag gaggtggcg 
      tcgacgtac ggcgacgga gacaccaac tcgcaaaac 
      gaccgctcc ggctgtttc tcccccaac ggcgtttcc 
      ggcaacttg cagtacccc gcgtacttt aaagacgcc 
      aaaggcatg aaccactct gtgttcggg tcgagtacg 
      aactttggt gcgttgcaa tacctcaag cagcagcaa 
      gcgggcaaa gtcctgccc gatgtcatc cagcacggg 
      cgctcggcg gggatgccc ctctacgtg cggtgtccc 
      atctgccag aaggagttc aagcagaag tcgacgctg 
      ctgcagcac gggtgtata catatcgag tcgaggccg 
      tatccgtgc ccggaatgc gggaagagg ttccgacag 
      cagtcgcat ctcacgcag cacttgagg attcatacg 
      aacgaaaaa ccctacggg tgcgtctac tgcggcagg 
      aatttcaga cagaggacc attttgaat cagcatttg 
      aggatacac accggggag aagccgtac aagtgtgga 
      cagtgcggg aaggacttt cggcagaag gcgatcctg 
      gatcagcac acgaggacg catcaaggt gaccgacca 
      ttctgttgc ccgatgccc aactgccgg cgccgcttc 
      gccaccgag cccgaggtc aagaagcac atcgacaac 
      cacatgaac ccgcatgct gcgaagacg cgccgcgac 
      tccaccggc tccgacggc aagctgaca gccaatggg 
      atgctgcca cgcggcctc acccccacc accgtggtc 
      aagcccgag ctgtacttc ccccagtgt tacgcgccc 
      agcttcaac cctccaacc agcgtagcc cagttccag 
      gctgcgaac ccggaattc aagccgccc aacgcgctg 
      ccgacccag tgactaacg gcgagctgg caactgggt 
      ccgtgtatt gggtgaccc tttgccagt ctatggact 
      gaacccgac acttagttg caggcttaa cggactcat 
      ccgctagtt aagttatat atttattgg tgatttcaa 
      tgaaatatc gacgagata ttttgctga gtgattatc 
      acatggtgc tgagaacca ctacactgg ttcactgta 
      acatacgga atgtagcac ttaacgcga taagtatac 
      atactataa atatatgta aaatgtaaa agtatttta 
      aattttatc cagacgtgt cctgttcac atagtggca 
      atactttta agaacaaag tactttcat aaggacagt 
      ctgggacct ggccgacga cttacttac acgatttta 
      gacacgttg aattttgta gaaaaatca ttcatactt 
      gaatcgctt atttaagaa aaaataaga gattttggt 
      acaagaatt atatttaca caacttgaa acaaccgaa 
      aatttttag tctttaaat tagcgtcat tttttagca 
      acttttttc aaattgacc gcctctggg aatcttaac 
      tttcgtttt ttgtctgag ctcagaatt cacattttc 
      ccgtttttt ttttctaaa atgcaaaaa tacctagat 
      gatgcaaca gagacaaac ttatcagca gaattttaa 
      cgttttaat gttaagact cctacgcct cttgtagtg 
      aattcctaa attttaatt tgaagaagg aatacttca 
      tttttcaga gctgaatac aaaattttg ccctttttt 
      aaaatctta taggaaggc tgtactttc aaagaacag 
      tgtttttgc attttttgg actaaacta gagcataat 
      tt