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Gene information
General information
TC002617 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid22: 1748013..1758574
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002617
    iBeetle screen iB_03419
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    procephalic appendages not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    gnathal appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs segment sometimes shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs segment sometimes potentiallyfused - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002617 RA
      MANENVKLK PLGTNKYTD GEGGGDGNS PSSNEDYDP 
      HLYREVENP TTNIETLIH LLKGCLGTG ILAMPEAFK 
      NSGLLNGLV STFLIGALC TYCLHVLVK AQYVMCKRL 
      RVPILSYPQ SMKVALESG PQCLRPFAK YSPLLVDFF 
      LIAYQLGIC CVYIVFVGV NVKVVVDQY LGKASITIY 
      ILCTFIPFL LINCIRNLK LLAPFSTLA NIITLASFG 
      VVCYYVFQD LPDISDRPS FGRLYTYPL FFGTTLFAL 
      EAVGVVIAL ENNMKTPKN FGGYCGVLN IGMVVVTVL 
      YVGLGFIGY WKYGDDVQA SLTLNFPIH EPMAQAISI 
      LYSIAIFIS YGLQGYVPV AIIWNTYIV KRLEGSSHL 
      LAWEYLLRF ACVIVTFVL ALTIPMLGL FISLFGAFC 
      LSALGFAFP AIMEICVYW PDNLGPFRW VLIKDVLLI 
      LVGVVGLLA GSYSCISEM VAEFQRLAA EGN
    CDS sequence
    • >TC002617 RA
      atggcgaac gaaaacgtt aaactgaag cctttgggc 
      accaacaaa tacacggat ggggaagga ggtggagat 
      ggaaattct ccaagttcg aacgaggat tacgaccct 
      cacctttac agggaggtg gagaatcct accacgaat 
      attgagact ctcattcat ttgctcaaa ggctgtcta 
      gggactggc attctggcg atgcccgaa gctttcaaa 
      aactcgggg cttttaaat ggattggtc tcgaccttt 
      ctcattggt gctctttgt acatactgt ttacacgtt 
      ctagttaaa gctcaatat gttatgtgc aaacggctg 
      agagttcca attttgagc tacccgcaa tcgatgaaa 
      gttgcacta gagtcaggt ccacagtgc cttaggcca 
      tttgcgaaa tattcaccc ttgttggtc gatttcttc 
      ttgatagcg taccagtta ggcatctgt tgcgtttac 
      atagtcttc gtcggcgtt aacgttaaa gtcgtagtg 
      gatcaatat ttaggaaaa gcgagtata acgatatac 
      atcctgtgt acctttatt ccattcttg ctgatcaac 
      tgtataaga aatttaaaa ctactagca ccgttctca 
      accctcgca aacatcata actttggct tctttcggt 
      gttgtgtgt tattatgtt ttccaagat ttgccagat 
      attagcgat aggccaagt tttgggcga ctatacacc 
      tatccattg ttctttggt accacttta ttcgcccta 
      gaagcggtc ggagtggtc atagcattg gaaaacaac 
      atgaagaca ccgaaaaat tttgggggt tattgtgga 
      gtcttaaac attggtatg gtggttgtt actgttctt 
      tacgtcggg ttgggattc atcggttat tggaaatac 
      ggtgacgat gttcaagct tccctcact ttaaatttt 
      ccaattcat gagccaatg gctcaagcc atcagcata 
      ctgtactca atcgccata ttcatctct tacggactt 
      caagggtat gttcctgtc gcaatcata tggaacacc 
      tatatcgtg aaaagactt gaaggaagt agccatctt 
      cttgcttgg gagtatcta ttacgcttt gcttgtgtg 
      attgtaacg tttgtgctc gctttgacc atccccatg 
      ctgggcctg ttcatttcc ctcttcggg gctttctgt 
      ttgtctgcg ttgggtttc gcgttcccg gcaatcatg 
      gaaatttgc gtgtactgg cccgacaac ctcggccca 
      ttccgctgg gttctgatc aaggatgtt ctcctcata 
      ctggttggt gttgtggga ctgctcgct gggagctac 
      agttgtata agtgaaatg gtagccgaa tttcagagg 
      ctggctgct gaaggaaat tag
    mRNA sequence
    • >TC002617 RA
      caagccatc agtctatca gagggtcgc gaacgcctg 
      acatcctct gtgtaaatt agattcgtt cgcatatac 
      agttacaag tgttattta cttaattgc taagaagtg 
      cacgcaatt ccagtttgt tagacgctt acaacgaaa 
      atgcgaacc gtctgacga tttctgaga tggcgaacg 
      aaaacgtta aactgaagc ctttgggca ccaacaaat 
      acacggatg gggaaggag gtggagatg gaaattctc 
      caagttcga acgaggatt acgaccctc acctttaca 
      gggaggtgg agaatccta ccacgaata ttgagactc 
      tcattcatt tgctcaaag gctgtctag ggactggca 
      ttctggcga tgcccgaag ctttcaaaa actcggggc 
      ttttaaatg gattggtct cgacctttc tcattggtg 
      ctctttgta catactgtt tacacgttc tagttaaag 
      ctcaatatg ttatgtgca aacggctga gagttccaa 
      ttttgagct acccgcaat cgatgaaag ttgcactag 
      agtcaggtc cacagtgcc ttaggccat ttgcgaaat 
      attcaccct tgttggtcg atttcttct tgatagcgt 
      accagttag gcatctgtt gcgtttaca tagtcttcg 
      tcggcgtta acgttaaag tcgtagtgg atcaatatt 
      taggaaaag cgagtataa cgatataca tcctgtgta 
      cctttattc cattcttgc tgatcaact gtataagaa 
      atttaaaac tactagcac cgttctcaa ccctcgcaa 
      acatcataa ctttggctt ctttcggtg ttgtgtgtt 
      attatgttt tccaagatt tgccagata ttagcgata 
      ggccaagtt ttgggcgac tatacacct atccattgt 
      tctttggta ccactttat tcgccctag aagcggtcg 
      gagtggtca tagcattgg aaaacaaca tgaagacac 
      cgaaaaatt ttgggggtt attgtggag tcttaaaca 
      ttggtatgg tggttgtta ctgttcttt acgtcgggt 
      tgggattca tcggttatt ggaaatacg gtgacgatg 
      ttcaagctt ccctcactt taaattttc caattcatg 
      agccaatgg ctcaagcca tcagcatac tgtactcaa 
      tcgccatat tcatctctt acggacttc aagggtatg 
      ttcctgtcg caatcatat ggaacacct atatcgtga 
      aaagacttg aaggaagta gccatcttc ttgcttggg 
      agtatctat tacgctttg cttgtgtga ttgtaacgt 
      ttgtgctcg ctttgacca tccccatgc tgggcctgt 
      tcatttccc tcttcgggg ctttctgtt tgtctgcgt 
      tgggtttcg cgttcccgg caatcatgg aaatttgcg 
      tgtactggc ccgacaacc tcggcccat tccgctggg 
      ttctgatca aggatgttc tcctcatac tggttggtg 
      ttgtgggac tgctcgctg ggagctaca gttgtataa 
      gtgaaatgg tagccgaat ttcagaggc tggctgctg 
      aaggaaatt agcacaagt gtcaaaatc agtgtattg 
      tgtgtttgc tgtttatgt tatttttat ttgtgtatg 
      tttttcttt gacatggat gatgtagaa attacaatc 
      taagatctg cctttacat ctgtccaag acgttacgc 
      tgtcgcaaa catatcata attgtttcg gtagtctga 
      tgatgtaga agtagtgat gtgcataca ttttttaat 
      gttctgttg ttttaagta acgagttcc agaataaaa 
      gacaaaatt gttgtgttg cgtttgtgt aactgtttg 
      tcctcaatg atacgctca aaacaatca ccaaatacg 
      taggatctt taacaacca acttaaagt aggtaacga 
      gcatatttt tattttagt ctaactatg tctaatgat 
      aatttaaga accgatcta gattttaaa aacaaagct 
      ggagacaat taatgaata gggctattt ttaagttct 
      cgactaaaa cgcacattt atgaacaaa ttcttatgt 
      aattagttg acgatcaat atttataaa taaaattta 
      tttgtaggt gtttgagtt tactaacta ccaactccc 
      aacttgtaa ccaaatcat gctatgttc agaaaacga 
      cgtgaaatt tgtagttca atatttatt taatttcgt 
      tgggataga gatagtttc tactgacaa aatgtctaa 
      aagaagtaa ctaggaatt aagtaatta ggaaatata 
      ggtgatgta gatcaattg agagtcgtt taattgcag 
      tggctttgt agggatagt gtgtggcaa aaattaagt 
      gtaacgaaa ttgtagcaa gccaatgat ttggccata 
      tttttatag tacgttgta gtaacagaa acgatacca 
      tactcattt aaattcgta tcatttttt gaatgttaa 
      gttgctgta aactgctgt gttcgttaa tgtgttaat 
      acaatatac aaaatattc aggtagatg tgcaatttc 
      aggattatt tcagagcgc tgtattgct ggataatga 
      aataagtga atcttgtaa aattatttt ataaaacgt 
      tgttacttt ctaaaaata cattgttgt gtttattgt 
      gactatagt tttttaata aaatgcttg atatgtt