Status
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Gene information
General information
TC032565
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002613,TC002614 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 21484900..21498509
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032565
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03418

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032565 RA
      MSGKLLLVV LLTFSANSH AYYYQDKIE YYVQLITGA 
FRNALTTVL PTDSYQYFS GEVRRSYGS FDFVVIGAG 
AAGAVIANR LTEVEDWNV LVLEAGGYG NDFSDIPDM 
YWPIEFTDF NWGYNSTPQ RTACLGLID QECFYPRGR 
GVGGSTLIN GLIYSRGHK TDFDHWGRL VGNDRWSYR 
SVLQYFKKS ENFVYRDYT QPIEPEYHG TNGYWQVEH 
HLPRSPQLD VFLDANREM GLGVADYNA NRLGASSAQ 
LNTAFGRRM DTGKAFIRS VLKRPNLKV LTGSFVTRI 
VIDKFTRSA VGVEFTHGG SNYFVRAKK EVILSAGAF 
NTPQLLMLS GIGPGYHLQ ELGIEVIQD LEVGSTLRD 
NPTFYGVAF QTNYTEPIE PLENYIEQY FQGVGPLAI 
PGNNQGVGF YESSYTRGT GIPDLEFMF IPAVASTIL 
QQRAFRLTD QTYNDVYQF QDVGSTFGV YVIVLHSKS 
VGTVRLRSR DPFQFPLID ANFLSDPEN KDINVLYEG 
VQLLMQMAQ TRAFRSMDA TLAGGQLSA CSQYEFLSR 
EYWYCAIRQ LTINVYHPL GTCPMGRDP REGAVVDSE 
LKVFGIKKL RVADSILTG SFVTRIVID KFTRSAVRV 
EFTHGGSNY FVRAKKEVI LSAGAFNTP QLLMLSGIG 
PGYHLQDLG IEVIQDLEV GSTLRDNPT FYGVAFQTN 
YTEPIEPLE NYIEQYFQG VGPLAIPGN NQGVGFYES 
SYTRGTGIP DLEFMFIPA VASTILQQR AFRLTDQTY 
NDVYRFQDV GSTFGVYVI VLHSKSVGT VRRRSRDPF 
QFPLIDANF LSDPENKDI NVLYEGVQL LMQMAQTRA 
FRSMDATLA GGQLSACSQ YEFLSREYW YCAIRQLTI 
NVYHPLGTC PMGRDPREG AVVDSELKV FGIKKLRVA 
DSSVFPFAL AGHPTAPSV MVGEQMGDI LKEKYKYND 
NYYDVFHDY L
    CDS sequence
    • >TC032565 RA
      atgagtgga aaactgctc ctggtggtg ttactaaca 
ttttccgcg aattcgcac gcttattac taccaagac 
aaaatcgaa tattatgtg cagttgata acgggtgcg 
tttcgcaac gccctgacc actgtgtta cccacggac 
tcctaccag tatttttcc ggcgaagtc agaagaagc 
tatggaagc ttcgacttt gtggtcatt ggagccgga 
gcggctggt gccgtgatt gcgaatcgt ctgactgaa 
gtggaggat tggaacgtt cttgttctt gaagccggc 
ggatatgga aatgatttt tccgacatt cctgatatg 
tactggcca atcgaattc acggatttc aactggggc 
tacaacagc acgccgcag cgaactgct tgcttaggt 
ctaatcgac caggaatgt ttctacccc cgaggaaga 
ggcgtcggt ggctcaacc ctaatcaac ggtctaatc 
tactcccgc ggccacaaa accgacttc gaccactgg 
ggccgactg gtcggaaac gaccgttgg tcgtaccgt 
agcgtcctc cagtacttc aaaaaatcg gaaaacttc 
gtataccgc gactacacc caaccgatc gaacccgaa 
taccatgga acaaacggc tactggcaa gtcgaacac 
cacttaccc agaagccca caactggac gttttcctg 
gacgccaac agagagatg ggtttaggc gtcgccgac 
tacaatgcc aacagattg ggcgcctca agtgcccaa 
ctgaacaca gctttcggc agacggatg gacacaggc 
aaagctttt atccggtct gttttaaaa cgcccgaac 
ttgaaagta ctaaccggg agtttcgtc acaagaatt 
gtcattgat aagtttaca cgtagtgct gtgggagtt 
gaattcact catggaggg agcaattat ttcgtaagg 
gcgaagaag gaggtgatt ttgtcagcg ggggctttc 
aatacgcct caattgttg atgttgtct gggataggg 
ccagggtac cacttacaa gaactagga attgaggtg 
atccaggat ctggaagtt gggagtacg ctcagggat 
aaccctact ttttacggg gttgcgttc cagacgaat 
tacaccgaa ccgattgaa cctctcgag aattatatc 
gaacagtac tttcaagga gttggtcct ttagccatc 
cctggaaat aaccaagga gtaggtttt tacgaatca 
agttatacc agaggtacc ggaattccg gatctcgaa 
ttcatgttt atcccagct gtggcttca acaatttta 
caacaaaga gccttcagg ttaaccgac caaacttac 
aacgacgtg taccagttc caggacgtg ggaagtacc 
ttcggtgtt tacgtcata gttttgcat tcaaaatct 
gtcggaact gtccgactc aggagcagg gatcccttc 
cagttcccg ctgattgac gcaaatttt ctctcagat 
cctgaaaat aaagacata aacgtgctt tatgaagga 
gtacagtta cttatgcaa atggcccaa accagggct 
ttcaggtca atggacgca actttggct ggaggtcag 
ttgtcggct tgtagccag tatgagttt ttgagtaga 
gagtactgg tactgtgcc attcgacaa cttaccatc 
aatgtgtac catccgctg ggtacttgt cccatggga 
agggatcca agggaagga gctgtggtg gattcggaa 
ttgaaggtt ttcgggatc aagaagtta agagtggct 
gattcaata ctaaccggg agtttcgtc acaagaatt 
gtgatcgat aagtttaca cgtagtgct gtgagagtt 
gaattcact catggaggg agcaattat ttcgtaagg 
gcgaagaag gaggtgatt ttgtcagcg ggggctttc 
aatacgcct caattgttg atgttgtct gggataggg 
ccagggtac cacttacaa gatctagga attgaggtt 
atccaggat ctggaagtt gggagtacg ctcagggat 
aaccctact ttttacggg gttgcgttc caaacaaat 
tacaccgaa ccaattgaa cctctcgag aattatatc 
gaacagtac tttcaagga gttggtcct ttagccatc 
cctggaaat aaccaagga gtaggtttt tacgaatca 
agttatacg agaggtacc ggaattccg gatctcgaa 
ttcatgttt atcccagct gtggcttca acaatttta 
caacaaaga gccttcagg ttaaccgac caaacttac 
aacgacgtg taccggttc caagacgtg ggaagtacc 
ttcggtgtt tacgtcata gttttgcat tcaaaatcg 
gtcggaact gtccgacgc aggagcagg gatcccttc 
cagttcccg ctgattgac gcaaatttt ctctcagat 
cccgaaaat aaagacata aacgtgctt tatgaagga 
gtacagtta cttatgcaa atggcccag accagggcc 
ttcaggtca atggacgca actttggct ggaggtcag 
ttgtcggct tgtagccag tatgagttt ttgagtaga 
gagtactgg tactgtgcc attcgacaa cttaccatc 
aatgtgtac catccgctg ggtacttgt cccatggga 
agggatcca agggaagga gctgtggtg gattcggaa 
ttgaaggtt ttcgggatc aagaagtta agagtggct 
gattcaagt gtgtttccg tttgctctg gctggtcat 
ccaactgca ccttcggtt atggttggc gaacaaatg 
ggtgatata cttaaagaa aagtataaa tacaatgac 
aattattat gatgttttt catgattat ttgtag
    mRNA sequence
    • >TC032565 RA
      ttttagtat gtgctggtt cgtttaaga atgagtgga 
aaactgctc ctggtggtg ttactaaca ttttccgcg 
aattcgcac gcttattac taccaagac aaaatcgaa 
tattatgtg cagttgata acgggtgcg tttcgcaac 
gccctgacc actgtgtta cccacggac tcctaccag 
tatttttcc ggcgaagtc agaagaagc tatggaagc 
ttcgacttt gtggtcatt ggagccgga gcggctggt 
gccgtgatt gcgaatcgt ctgactgaa gtggaggat 
tggaacgtt cttgttctt gaagccggc ggatatgga 
aatgatttt tccgacatt cctgatatg tactggcca 
atcgaattc acggatttc aactggggc tacaacagc 
acgccgcag cgaactgct tgcttaggt ctaatcgac 
caggaatgt ttctacccc cgaggaaga ggcgtcggt 
ggctcaacc ctaatcaac ggtctaatc tactcccgc 
ggccacaaa accgacttc gaccactgg ggccgactg 
gtcggaaac gaccgttgg tcgtaccgt agcgtcctc 
cagtacttc aaaaaatcg gaaaacttc gtataccgc 
gactacacc caaccgatc gaacccgaa taccatgga 
acaaacggc tactggcaa gtcgaacac cacttaccc 
agaagccca caactggac gttttcctg gacgccaac 
agagagatg ggtttaggc gtcgccgac tacaatgcc 
aacagattg ggcgcctca agtgcccaa ctgaacaca 
gctttcggc agacggatg gacacaggc aaagctttt 
atccggtct gttttaaaa cgcccgaac ttgaaagta 
ctaaccggg agtttcgtc acaagaatt gtcattgat 
aagtttaca cgtagtgct gtgggagtt gaattcact 
catggaggg agcaattat ttcgtaagg gcgaagaag 
gaggtgatt ttgtcagcg ggggctttc aatacgcct 
caattgttg atgttgtct gggataggg ccagggtac 
cacttacaa gaactagga attgaggtg atccaggat 
ctggaagtt gggagtacg ctcagggat aaccctact 
ttttacggg gttgcgttc cagacgaat tacaccgaa 
ccgattgaa cctctcgag aattatatc gaacagtac 
tttcaagga gttggtcct ttagccatc cctggaaat 
aaccaagga gtaggtttt tacgaatca agttatacc 
agaggtacc ggaattccg gatctcgaa ttcatgttt 
atcccagct gtggcttca acaatttta caacaaaga 
gccttcagg ttaaccgac caaacttac aacgacgtg 
taccagttc caggacgtg ggaagtacc ttcggtgtt 
tacgtcata gttttgcat tcaaaatct gtcggaact 
gtccgactc aggagcagg gatcccttc cagttcccg 
ctgattgac gcaaatttt ctctcagat cctgaaaat 
aaagacata aacgtgctt tatgaagga gtacagtta 
cttatgcaa atggcccaa accagggct ttcaggtca 
atggacgca actttggct ggaggtcag ttgtcggct 
tgtagccag tatgagttt ttgagtaga gagtactgg 
tactgtgcc attcgacaa cttaccatc aatgtgtac 
catccgctg ggtacttgt cccatggga agggatcca 
agggaagga gctgtggtg gattcggaa ttgaaggtt 
ttcgggatc aagaagtta agagtggct gattcaata 
ctaaccggg agtttcgtc acaagaatt gtgatcgat 
aagtttaca cgtagtgct gtgagagtt gaattcact 
catggaggg agcaattat ttcgtaagg gcgaagaag 
gaggtgatt ttgtcagcg ggggctttc aatacgcct 
caattgttg atgttgtct gggataggg ccagggtac 
cacttacaa gatctagga attgaggtt atccaggat 
ctggaagtt gggagtacg ctcagggat aaccctact 
ttttacggg gttgcgttc caaacaaat tacaccgaa 
ccaattgaa cctctcgag aattatatc gaacagtac 
tttcaagga gttggtcct ttagccatc cctggaaat 
aaccaagga gtaggtttt tacgaatca agttatacg 
agaggtacc ggaattccg gatctcgaa ttcatgttt 
atcccagct gtggcttca acaatttta caacaaaga 
gccttcagg ttaaccgac caaacttac aacgacgtg 
taccggttc caagacgtg ggaagtacc ttcggtgtt 
tacgtcata gttttgcat tcaaaatcg gtcggaact 
gtccgacgc aggagcagg gatcccttc cagttcccg 
ctgattgac gcaaatttt ctctcagat cccgaaaat 
aaagacata aacgtgctt tatgaagga gtacagtta 
cttatgcaa atggcccag accagggcc ttcaggtca 
atggacgca actttggct ggaggtcag ttgtcggct 
tgtagccag tatgagttt ttgagtaga gagtactgg 
tactgtgcc attcgacaa cttaccatc aatgtgtac 
catccgctg ggtacttgt cccatggga agggatcca 
agggaagga gctgtggtg gattcggaa ttgaaggtt 
ttcgggatc aagaagtta agagtggct gattcaagt 
gtgtttccg tttgctctg gctggtcat ccaactgca 
ccttcggtt atggttggc gaacaaatg ggtgatata 
cttaaagaa aagtataaa tacaatgac aattattat 
gatgttttt catgattat ttgtagatt tgtagttgt 
gtaggtatt taattttaa taaaatatc tagtcaaat 
aaaggagta gtgggaggg gcattttgg gacaattat 
tggactttt tggtacaaa atctgattt tgtgtttaa 
ctactacaa tgaaaacct ttttgagac tgagaccag 
aaaaacatt taaatacag tgtactaat taatttaca 
atggtgttg taattttaa ttagtaaaa tagagaatg 
ttacttttg gatcagagt gaaaaaagg tcaaaaata 
tttcaaatt ctaaagact agcaaaaat tgcacacac 
gcatatata gttataaaa agtttgaac agctcaaaa 
tatattcac atgtctgga ccatgccaa ttgttaatt 
aagaacata ttatttatt tataaccga aatttatta 
gctctacgt aatctcgga ttttttgat ttggacact 
ttaaaagtc atcaaaaat aagccccca aaaattaac 
aaaaaagtc gaaatagct aattttttg acaaacaaa 
actttggat aaattaaac tttaaatat aggataacc 
ttatttgaa aataacaaa aagaggaca aagaaaatg 
caagaaaaa taaagaggg atgagtcac attatttct 
gaatagcaa atattacgt ataattttt ttaatcaat 
aaactcttt atttcaac

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY