| General information | |||
| TC032565
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 21484900..21498509 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC032565 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03418 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
remarks: animals lost,
- >TC032565 RA
MSGKLLLVV LLTFSANSH AYYYQDKIE YYVQLITGA FRNALTTVL PTDSYQYFS GEVRRSYGS FDFVVIGAG AAGAVIANR LTEVEDWNV LVLEAGGYG NDFSDIPDM YWPIEFTDF NWGYNSTPQ RTACLGLID QECFYPRGR GVGGSTLIN GLIYSRGHK TDFDHWGRL VGNDRWSYR SVLQYFKKS ENFVYRDYT QPIEPEYHG TNGYWQVEH HLPRSPQLD VFLDANREM GLGVADYNA NRLGASSAQ LNTAFGRRM DTGKAFIRS VLKRPNLKV LTGSFVTRI VIDKFTRSA VGVEFTHGG SNYFVRAKK EVILSAGAF NTPQLLMLS GIGPGYHLQ ELGIEVIQD LEVGSTLRD NPTFYGVAF QTNYTEPIE PLENYIEQY FQGVGPLAI PGNNQGVGF YESSYTRGT GIPDLEFMF IPAVASTIL QQRAFRLTD QTYNDVYQF QDVGSTFGV YVIVLHSKS VGTVRLRSR DPFQFPLID ANFLSDPEN KDINVLYEG VQLLMQMAQ TRAFRSMDA TLAGGQLSA CSQYEFLSR EYWYCAIRQ LTINVYHPL GTCPMGRDP REGAVVDSE LKVFGIKKL RVADSILTG SFVTRIVID KFTRSAVRV EFTHGGSNY FVRAKKEVI LSAGAFNTP QLLMLSGIG PGYHLQDLG IEVIQDLEV GSTLRDNPT FYGVAFQTN YTEPIEPLE NYIEQYFQG VGPLAIPGN NQGVGFYES SYTRGTGIP DLEFMFIPA VASTILQQR AFRLTDQTY NDVYRFQDV GSTFGVYVI VLHSKSVGT VRRRSRDPF QFPLIDANF LSDPENKDI NVLYEGVQL LMQMAQTRA FRSMDATLA GGQLSACSQ YEFLSREYW YCAIRQLTI NVYHPLGTC PMGRDPREG AVVDSELKV FGIKKLRVA DSSVFPFAL AGHPTAPSV MVGEQMGDI LKEKYKYND NYYDVFHDY L
- >TC032565 RA
atgagtgga aaactgctc ctggtggtg ttactaaca ttttccgcg aattcgcac gcttattac taccaagac aaaatcgaa tattatgtg cagttgata acgggtgcg tttcgcaac gccctgacc actgtgtta cccacggac tcctaccag tatttttcc ggcgaagtc agaagaagc tatggaagc ttcgacttt gtggtcatt ggagccgga gcggctggt gccgtgatt gcgaatcgt ctgactgaa gtggaggat tggaacgtt cttgttctt gaagccggc ggatatgga aatgatttt tccgacatt cctgatatg tactggcca atcgaattc acggatttc aactggggc tacaacagc acgccgcag cgaactgct tgcttaggt ctaatcgac caggaatgt ttctacccc cgaggaaga ggcgtcggt ggctcaacc ctaatcaac ggtctaatc tactcccgc ggccacaaa accgacttc gaccactgg ggccgactg gtcggaaac gaccgttgg tcgtaccgt agcgtcctc cagtacttc aaaaaatcg gaaaacttc gtataccgc gactacacc caaccgatc gaacccgaa taccatgga acaaacggc tactggcaa gtcgaacac cacttaccc agaagccca caactggac gttttcctg gacgccaac agagagatg ggtttaggc gtcgccgac tacaatgcc aacagattg ggcgcctca agtgcccaa ctgaacaca gctttcggc agacggatg gacacaggc aaagctttt atccggtct gttttaaaa cgcccgaac ttgaaagta ctaaccggg agtttcgtc acaagaatt gtcattgat aagtttaca cgtagtgct gtgggagtt gaattcact catggaggg agcaattat ttcgtaagg gcgaagaag gaggtgatt ttgtcagcg ggggctttc aatacgcct caattgttg atgttgtct gggataggg ccagggtac cacttacaa gaactagga attgaggtg atccaggat ctggaagtt gggagtacg ctcagggat aaccctact ttttacggg gttgcgttc cagacgaat tacaccgaa ccgattgaa cctctcgag aattatatc gaacagtac tttcaagga gttggtcct ttagccatc cctggaaat aaccaagga gtaggtttt tacgaatca agttatacc agaggtacc ggaattccg gatctcgaa ttcatgttt atcccagct gtggcttca acaatttta caacaaaga gccttcagg ttaaccgac caaacttac aacgacgtg taccagttc caggacgtg ggaagtacc ttcggtgtt tacgtcata gttttgcat tcaaaatct gtcggaact gtccgactc aggagcagg gatcccttc cagttcccg ctgattgac gcaaatttt ctctcagat cctgaaaat aaagacata aacgtgctt tatgaagga gtacagtta cttatgcaa atggcccaa accagggct ttcaggtca atggacgca actttggct ggaggtcag ttgtcggct tgtagccag tatgagttt ttgagtaga gagtactgg tactgtgcc attcgacaa cttaccatc aatgtgtac catccgctg ggtacttgt cccatggga agggatcca agggaagga gctgtggtg gattcggaa ttgaaggtt ttcgggatc aagaagtta agagtggct gattcaata ctaaccggg agtttcgtc acaagaatt gtgatcgat aagtttaca cgtagtgct gtgagagtt gaattcact catggaggg agcaattat ttcgtaagg gcgaagaag gaggtgatt ttgtcagcg ggggctttc aatacgcct caattgttg atgttgtct gggataggg ccagggtac cacttacaa gatctagga attgaggtt atccaggat ctggaagtt gggagtacg ctcagggat aaccctact ttttacggg gttgcgttc caaacaaat tacaccgaa ccaattgaa cctctcgag aattatatc gaacagtac tttcaagga gttggtcct ttagccatc cctggaaat aaccaagga gtaggtttt tacgaatca agttatacg agaggtacc ggaattccg gatctcgaa ttcatgttt atcccagct gtggcttca acaatttta caacaaaga gccttcagg ttaaccgac caaacttac aacgacgtg taccggttc caagacgtg ggaagtacc ttcggtgtt tacgtcata gttttgcat tcaaaatcg gtcggaact gtccgacgc aggagcagg gatcccttc cagttcccg ctgattgac gcaaatttt ctctcagat cccgaaaat aaagacata aacgtgctt tatgaagga gtacagtta cttatgcaa atggcccag accagggcc ttcaggtca atggacgca actttggct ggaggtcag ttgtcggct tgtagccag tatgagttt ttgagtaga gagtactgg tactgtgcc attcgacaa cttaccatc aatgtgtac catccgctg ggtacttgt cccatggga agggatcca agggaagga gctgtggtg gattcggaa ttgaaggtt ttcgggatc aagaagtta agagtggct gattcaagt gtgtttccg tttgctctg gctggtcat ccaactgca ccttcggtt atggttggc gaacaaatg ggtgatata cttaaagaa aagtataaa tacaatgac aattattat gatgttttt catgattat ttgtag
- >TC032565 RA
ttttagtat gtgctggtt cgtttaaga atgagtgga aaactgctc ctggtggtg ttactaaca ttttccgcg aattcgcac gcttattac taccaagac aaaatcgaa tattatgtg cagttgata acgggtgcg tttcgcaac gccctgacc actgtgtta cccacggac tcctaccag tatttttcc ggcgaagtc agaagaagc tatggaagc ttcgacttt gtggtcatt ggagccgga gcggctggt gccgtgatt gcgaatcgt ctgactgaa gtggaggat tggaacgtt cttgttctt gaagccggc ggatatgga aatgatttt tccgacatt cctgatatg tactggcca atcgaattc acggatttc aactggggc tacaacagc acgccgcag cgaactgct tgcttaggt ctaatcgac caggaatgt ttctacccc cgaggaaga ggcgtcggt ggctcaacc ctaatcaac ggtctaatc tactcccgc ggccacaaa accgacttc gaccactgg ggccgactg gtcggaaac gaccgttgg tcgtaccgt agcgtcctc cagtacttc aaaaaatcg gaaaacttc gtataccgc gactacacc caaccgatc gaacccgaa taccatgga acaaacggc tactggcaa gtcgaacac cacttaccc agaagccca caactggac gttttcctg gacgccaac agagagatg ggtttaggc gtcgccgac tacaatgcc aacagattg ggcgcctca agtgcccaa ctgaacaca gctttcggc agacggatg gacacaggc aaagctttt atccggtct gttttaaaa cgcccgaac ttgaaagta ctaaccggg agtttcgtc acaagaatt gtcattgat aagtttaca cgtagtgct gtgggagtt gaattcact catggaggg agcaattat ttcgtaagg gcgaagaag gaggtgatt ttgtcagcg ggggctttc aatacgcct caattgttg atgttgtct gggataggg ccagggtac cacttacaa gaactagga attgaggtg atccaggat ctggaagtt gggagtacg ctcagggat aaccctact ttttacggg gttgcgttc cagacgaat tacaccgaa ccgattgaa cctctcgag aattatatc gaacagtac tttcaagga gttggtcct ttagccatc cctggaaat aaccaagga gtaggtttt tacgaatca agttatacc agaggtacc ggaattccg gatctcgaa ttcatgttt atcccagct gtggcttca acaatttta caacaaaga gccttcagg ttaaccgac caaacttac aacgacgtg taccagttc caggacgtg ggaagtacc ttcggtgtt tacgtcata gttttgcat tcaaaatct gtcggaact gtccgactc aggagcagg gatcccttc cagttcccg ctgattgac gcaaatttt ctctcagat cctgaaaat aaagacata aacgtgctt tatgaagga gtacagtta cttatgcaa atggcccaa accagggct ttcaggtca atggacgca actttggct ggaggtcag ttgtcggct tgtagccag tatgagttt ttgagtaga gagtactgg tactgtgcc attcgacaa cttaccatc aatgtgtac catccgctg ggtacttgt cccatggga agggatcca agggaagga gctgtggtg gattcggaa ttgaaggtt ttcgggatc aagaagtta agagtggct gattcaata ctaaccggg agtttcgtc acaagaatt gtgatcgat aagtttaca cgtagtgct gtgagagtt gaattcact catggaggg agcaattat ttcgtaagg gcgaagaag gaggtgatt ttgtcagcg ggggctttc aatacgcct caattgttg atgttgtct gggataggg ccagggtac cacttacaa gatctagga attgaggtt atccaggat ctggaagtt gggagtacg ctcagggat aaccctact ttttacggg gttgcgttc caaacaaat tacaccgaa ccaattgaa cctctcgag aattatatc gaacagtac tttcaagga gttggtcct ttagccatc cctggaaat aaccaagga gtaggtttt tacgaatca agttatacg agaggtacc ggaattccg gatctcgaa ttcatgttt atcccagct gtggcttca acaatttta caacaaaga gccttcagg ttaaccgac caaacttac aacgacgtg taccggttc caagacgtg ggaagtacc ttcggtgtt tacgtcata gttttgcat tcaaaatcg gtcggaact gtccgacgc aggagcagg gatcccttc cagttcccg ctgattgac gcaaatttt ctctcagat cccgaaaat aaagacata aacgtgctt tatgaagga gtacagtta cttatgcaa atggcccag accagggcc ttcaggtca atggacgca actttggct ggaggtcag ttgtcggct tgtagccag tatgagttt ttgagtaga gagtactgg tactgtgcc attcgacaa cttaccatc aatgtgtac catccgctg ggtacttgt cccatggga agggatcca agggaagga gctgtggtg gattcggaa ttgaaggtt ttcgggatc aagaagtta agagtggct gattcaagt gtgtttccg tttgctctg gctggtcat ccaactgca ccttcggtt atggttggc gaacaaatg ggtgatata cttaaagaa aagtataaa tacaatgac aattattat gatgttttt catgattat ttgtagatt tgtagttgt gtaggtatt taattttaa taaaatatc tagtcaaat aaaggagta gtgggaggg gcattttgg gacaattat tggactttt tggtacaaa atctgattt tgtgtttaa ctactacaa tgaaaacct ttttgagac tgagaccag aaaaacatt taaatacag tgtactaat taatttaca atggtgttg taattttaa ttagtaaaa tagagaatg ttacttttg gatcagagt gaaaaaagg tcaaaaata tttcaaatt ctaaagact agcaaaaat tgcacacac gcatatata gttataaaa agtttgaac agctcaaaa tatattcac atgtctgga ccatgccaa ttgttaatt aagaacata ttatttatt tataaccga aatttatta gctctacgt aatctcgga ttttttgat ttggacact ttaaaagtc atcaaaaat aagccccca aaaattaac aaaaaagtc gaaatagct aattttttg acaaacaaa actttggat aaattaaac tttaaatat aggataacc ttatttgaa aataacaaa aagaggaca aagaaaatg caagaaaaa taaagaggg atgagtcac attatttct gaatagcaa atattacgt ataattttt ttaatcaat aaactcttt atttcaac
