Status
Please wait ...
Gene information
General information
TC002603 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid22: 2440835..2442454
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002603
    iBeetle screen iB_03414
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002603 RA
      MFVNKVHDQ KVELLRAVF RNLYLKEKC DYLDSECKM 
      LAEQILSSQ VTREKRNES KADLENEFN SLKLLQDEK 
      MHELAVANE KIVALSSVL ENSKLSLSE SKSMSEVLD 
      VKNEMIKCL QEELVQARL AAAEDWALI RELRQVRDE 
      LEKDKEMLT KTKPETIAR LQEELMTAK ILETQYSVE 
      FAEIRKHLE KIKAEIVAH KTECHLKPE GRIKLFARG 
      AKWVTDDEK KDTKEIEKE IKELEKEKS FLQNKIPDL 
      QAKIDVLSK HIKFKDEER RKLQEKLDS VKTKYQSIE 
      SITLERRRQ CEHLEHSAS EKVHIIRNS CSEKKQNID 
      DLSNKLGEI SDRRQSITK ELKQKELTT IQGLEEKIR 
      ILEQRIKQL NAEEEEWRE SAIITENDL SP
    CDS sequence
    • >TC002603 RA
      atgtttgtc aataaagtg cacgaccaa aaagtagaa 
      ctactgcgc gctgttttt cgtaatttg tatctgaaa 
      gaaaagtgc gattacttg gacagcgaa tgcaagatg 
      ctagccgaa caaatcctc tcaagtcag gtgactcgg 
      gagaaacgt aatgaatct aaagccgat ctagagaac 
      gaattcaat agtttgaag cttttgcaa gacgaaaaa 
      atgcacgaa ttggcggtt gccaacgag aaaattgtt 
      gcactatct tcagtattg gaaaattcg aaattatct 
      ctgagtgag agcaaatcg atgagtgag gttctggac 
      gtaaagaat gagatgata aagtgttta caagaggaa 
      ttagttcaa gcgcgatta gcggcggcc gaagattgg 
      gctctaatc cgagaattg agacaagtt agagacgaa 
      ttggaaaaa gataaagaa atgttaact aagacaaag 
      ccggaaact attgcacga cttcaggag gaactaatg 
      actgctaaa atattagag acgcaatat tcggtcgaa 
      tttgccgaa attagaaaa catttggag aaaattaaa 
      gccgaaatt gttgcccat aaaacggaa tgccatttg 
      aagcctgaa ggtaggatc aaattattc gcaagaggg 
      gcaaaatgg gtgactgat gatgaaaag aaagacact 
      aaagagata gaaaaagaa ataaaagaa ttggaaaaa 
      gaaaaatcg tttcttcaa aataaaatt cccgatctg 
      caagctaaa atcgatgtt ttgagcaaa cacataaag 
      ttcaaagac gaagaacgt cgcaaactg caagaaaaa 
      ttagacagt gtgaagaca aaataccaa agtattgag 
      agcataact ctggaacgg aggagacaa tgtgaacat 
      ttagagcac agtgcgtct gaaaaagta cacataata 
      cgtaatagt tgtagcgag aagaagcaa aacattgat 
      gatctttct aataaactt ggcgagata tcggaccga 
      agacagagt attacaaag gaattgaaa caaaaggag 
      ttaacgact attcaaggc cttgaggaa aaaattcgt 
      attttagaa caaagaatt aaacagtta aatgcagag 
      gaagaagaa tggcgtgaa tcagcgatt attacggaa 
      aatgatttg tctccttga
    mRNA sequence
    • >TC002603 RA
      agataaggt actgttgca atggctaaa taaaaatgt 
      ttgtcaata aagtgcacg accaaaaag tagaactac 
      tgcgcgctg tttttcgta atttgtatc tgaaagaaa 
      agtgcgatt acttggaca gcgaatgca agatgctag 
      ccgaacaaa tcctctcaa gtcaggtga ctcgggaga 
      aacgtaatg aatctaaag ccgatctag agaacgaat 
      tcaatagtt tgaagcttt tgcaagacg aaaaaatgc 
      acgaattgg cggttgcca acgagaaaa ttgttgcac 
      tatcttcag tattggaaa attcgaaat tatctctga 
      gtgagagca aatcgatga gtgaggttc tggacgtaa 
      agaatgaga tgataaagt gtttacaag aggaattag 
      ttcaagcgc gattagcgg cggccgaag attgggctc 
      taatccgag aattgagac aagttagag acgaattgg 
      aaaaagata aagaaatgt taactaaga caaagccgg 
      aaactattg cacgacttc aggaggaac taatgactg 
      ctaaaatat tagagacgc aatattcgg tcgaatttg 
      ccgaaatta gaaaacatt tggagaaaa ttaaagccg 
      aaattgttg cccataaaa cggaatgcc atttgaagc 
      ctgaaggta ggatcaaat tattcgcaa gaggggcaa 
      aatgggtga ctgatgatg aaaagaaag acactaaag 
      agatagaaa aagaaataa aagaattgg aaaaagaaa 
      aatcgtttc ttcaaaata aaattcccg atctgcaag 
      ctaaaatcg atgttttga gcaaacaca taaagttca 
      aagacgaag aacgtcgca aactgcaag aaaaattag 
      acagtgtga agacaaaat accaaagta ttgagagca 
      taactctgg aacggagga gacaatgtg aacatttag 
      agcacagtg cgtctgaaa aagtacaca taatacgta 
      atagttgta gcgagaaga agcaaaaca ttgatgatc 
      tttctaata aacttggcg agatatcgg accgaagac 
      agagtatta caaaggaat tgaaacaaa aggagttaa 
      cgactattc aaggccttg aggaaaaaa ttcgtattt 
      tagaacaaa gaattaaac agttaaatg cagaggaag 
      aagaatggc gtgaatcag cgattatta cggaaaatg 
      atttgtctc cttgaattt gattgtgtt gatgttttt 
      gaagtgttc tgaacgttt tatggccaa attttacca 
      tcttgccgt aacagtaat tgatttttg tattttttg 
      tataaacgt acgattgac taaataaat aataaaaaa 
      gtat