General information | |||
TC002602 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 22125276..22204395 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC002602 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03413 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 7 (22 dpi)
adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
adult lethality - Number of animals showing the phenotype: 5 (41 dpi)
motility decreased - Number of animals showing the phenotype: 9 (19 dpi)
- >TC002602 RA
MPPTAMRLE SPPCNNNNN ASGAELELL AKLEEANRL YESDAKSLN SLSGASGHS RKSSDTSQI SLTSGTSST QDRAEDCGE EDLWTTWGR IVNDWENNW KKQNAQIRE LVRKGIPLH FRAIVWQLL CSAAEAPEK KLYAEYIKT KSPCEKVIR RDIARTYPE HDFFKEKDG LGQESLFNV IKAYSLHDR EVGYCQGSG FIVGLLLMQ MPEEEAFAV LVKIMQDYR MRDMFKPSM AELGLCMYQ LENLVAEYL PDLNQHFQS QNFHTSMYA SSWFLTLFT TALSLPLAC RIMDVFLSE GMEIIFKVA LAMLTLGKD ELMSLDMEG MLKFFQKEL PVKAEADPE NLMQVAYNM KYNAKKMKK LEKEYNVIK TKEQEEMAE LKRLRTENK QLKHRCEML EEESKALAD RLVRGQVSR AEEEETTFV VQRELDALR HTHLETTHQ LALANEKIR SLSLMMEET HTSRQSSID EISLKQEQL QQREEMIEC LQEELVKVR LREAENDAL IRDLRARIH ELEEDKKTL REITPDNSV AHLQEELIA VKLREAEAN LSLKDLRQR VSELSNQWQ RHLQEHKQE PTASGEPHS TPKKLLFWE NRNNETQKL EDELMTTRI REMETLTEV KELRLKVME LETQVQVST NQLRRQDEE IKKQRDELE KAELRDREA AARLLEEHR RYSDLESKM QDELMKARI SDAEKTQAI AELTQKISQ LELKSQEIA TEGELRIQS VDDSDRVRE LQDKVADLQ AEVMRLESF KRRIIAGGG RLPSERSCS IDSTDDDPK IRLDDPSLR LNLVESPTS KSELTFSDM CQNPTIKND NKVDLDPNM NTHTTIDAH EEKCAPKV
- >TC002602 RA
atgcctccg acagccatg aggctcgag agccctcct tgcaacaat aataacaac gcatctgga gcagaactt gaacttctc gccaaactg gaggaagca aaccgtctt tacgagtcc gacgccaag tccctcaat tcgctgagc ggggcgtcg gggcactcg aggaaaagt tccgacact tcgcaaata tcgctaacc tcgggcacg agctccaca caagatcga gctgaggat tgcggtgag gaggatttg tggaccact tgggggcgt attgtcaat gattgggag aataattgg aagaagcag aatgctcaa atcagggaa ttggtgcgg aagggcatt ccgttacat ttcagggcg atagtttgg caattgctg tgcagtgca gccgaagct cctgaaaag aaactctac gccgaatat atcaagaca aaatctccg tgtgaaaaa gtcatccgt cgagacatc gcccgcact taccccgag cacgacttc ttcaaagaa aaagatggt cttggccaa gaatctctc ttcaacgtc atcaaagcc tactcccta cacgatcgg gaagtgggt tattgccag ggatcgggt tttatcgtc ggactttta cttatgcaa atgccagaa gaggaagct ttcgctgtg ctcgttaaa attatgcaa gattaccgc atgcgtgac atgttcaaa ccgagtatg gcagaattg ggcctttgt atgtaccag ctcgagaac ctggtcgct gaatatttg ccagacctc aaccaacat ttccagtca cagaatttc cacacttcg atgtatgct tccagttgg tttctgaca ctgtttacc accgctttg tctctgccg ctagcgtgc aggatcatg gatgttttt ttatcagag ggaatggag atcattttc aaagtggca cttgccatg ttgacgttg ggcaaagac gaactcatg tcgctcgat atggaggga atgttgaag tttttccaa aaagaactt cccgtcaaa gccgaagct gacccggaa aaccttatg caagtagcc tacaatatg aaatacaat gccaagaaa atgaaaaaa ttggaaaaa gaatacaat gtgataaaa acaaaggaa caagaggaa atggctgaa ttgaaacgc ttgagaacc gaaaacaag cagttgaaa catcgctgc gaaatgttg gaggaagaa agcaaagct ctagctgat cgtttggtc cgcggtcaa gtcagcaga gctgaagaa gaagaaact acttttgta gttcaacgt gaattggac gctttaaga cacactcat ttggaaacg acacaccaa ttagcttta gcgaacgaa aaaattagg tcattgtct ttgatgatg gaagaaact cacacttcg agacaatct tccatcgac gaaatttcc ctcaaacaa gagcaactg caacaaagg gaagaaatg atcgaatgc ctccaagaa gaactggtc aaagtgcga ttacgtgaa gctgaaaac gacgcttta attcgcgac ttaagagcc agaatacac gagttggaa gaagacaaa aaaacactc cgtgaaata acaccagac aatagtgtt gcccacctc caggaagag ctaatcgct gtgaagctc cgcgaagct gaagctaac ctctctttg aaggatttg cgccaaaga gtgtcagaa cttagcaat cagtggcag cgccacctc caggagcac aaacaggag ccgactgcc tccggggaa ccacacagc actcccaag aaactcctc ttctgggag aaccgaaac aatgagacg cagaagttg gaggacgag cttatgacc actaggata cgagaaatg gagactttg actgaagtg aaggagttg aggcttaag gtgatggag cttgagact caggtgcag gtcagtacg aatcagttg agacgccag gatgaggaa attaagaag cagagagat gagttggag aaggctgag ttgagggat cgggaagct gcggctcgt ttgttggag gagcatcgc cggtattcg gatcttgag agtaaaatg caagatgag ttgatgaag gcgaggatt agcgatgcg gagaaaacg caagctatt gccgagctc acgcaaaag atcagccag ctggagttg aagagccag gaaattgcc accgaaggc gagcttcga atccagtcg gtcgacgat agcgatcga gttagagaa ttgcaagat aaagtggct gatcttcaa gccgaggtc atgagattg gaatcgttc aagagacga ataatcgcc gggggtggt cgtctgccg tcggagagg tcttgttcc atcgacagc accgacgac gatcccaag atcagattg gacgatcct tccctgaga ttaaactta gtcgagtct cctacctcc aaatccgag cttactttt agcgacatg tgtcaaaat cctactata aaaaacgac aacaaagta gatctagat cccaacatg aatacgcac accactata gatgctcac gaagagaag tgtgcccca aaggtctga
- >TC002602 RA
catacaatt ttgtaatca gctgggcgg tgacagttc gattttgga catttgcgt ggttaggta tcgacgcgt gcgacgcta gacacgtca aatacggcg attactgtt tttatttat ctcttcgtt tttaattta tttggaaag ctttaattt tgtgtgaga actgggaga ttgttaatg tgacgatat tgtaaagtt tgattttat ttgtttcga attcgtcaa gtggttgtt ggtgaaagt gtgggtgtt cttttatgg taaacaaaa acatattct agggttttc ggatcattt tagtggtgg tggatcgtt gagctgtct cgtggtcga gtccgatgg aggctgcta ctcagcaat gcctccgac agccatgag gctcgagag ccctccttg caacaataa taacaacgc atctggagc agaacttga acttctcgc caaactgga ggaagcaaa ccgtcttta cgagtccga cgccaagtc cctcaattc gctgagcgg ggcgtcggg gcactcgag gaaaagttc cgacacttc gcaaatatc gctaacctc gggcacgag ctccacaca agatcgagc tgaggattg cggtgagga ggatttgtg gaccacttg ggggcgtat tgtcaatga ttgggagaa taattggaa gaagcagaa tgctcaaat cagggaatt ggtgcggaa gggcattcc gttacattt cagggcgat agtttggca attgctgtg cagtgcagc cgaagctcc tgaaaagaa actctacgc cgaatatat caagacaaa atctccgtg tgaaaaagt catccgtcg agacatcgc ccgcactta ccccgagca cgacttctt caaagaaaa agatggtct tggccaaga atctctctt caacgtcat caaagccta ctccctaca cgatcggga agtgggtta ttgccaggg atcgggttt tatcgtcgg acttttact tatgcaaat gccagaaga ggaagcttt cgctgtgct cgttaaaat tatgcaaga ttaccgcat gcgtgacat gttcaaacc gagtatggc agaattggg cctttgtat gtaccagct cgagaacct ggtcgctga atatttgcc agacctcaa ccaacattt ccagtcaca gaatttcca cacttcgat gtatgcttc cagttggtt tctgacact gtttaccac cgctttgtc tctgccgct agcgtgcag gatcatgga tgttttttt atcagaggg aatggagat cattttcaa agtggcact tgccatgtt gacgttggg caaagacga actcatgtc gctcgatat ggagggaat gttgaagtt tttccaaaa agaacttcc cgtcaaagc cgaagctga cccggaaaa ccttatgca agtagccta caatatgaa atacaatgc caagaaaat gaaaaaatt ggaaaaaga atacaatgt gataaaaac aaaggaaca agaggaaat ggctgaatt gaaacgctt gagaaccga aaacaagca gttgaaaca tcgctgcga aatgttgga ggaagaaag caaagctct agctgatcg tttggtccg cggtcaagt cagcagagc tgaagaaga agaaactac ttttgtagt tcaacgtga attggacgc tttaagaca cactcattt ggaaacgac acaccaatt agctttagc gaacgaaaa aattaggtc attgtcttt gatgatgga agaaactca cacttcgag acaatcttc catcgacga aatttccct caaacaaga gcaactgca acaaaggga agaaatgat cgaatgcct ccaagaaga actggtcaa agtgcgatt acgtgaagc tgaaaacga cgctttaat tcgcgactt aagagccag aatacacga gttggaaga agacaaaaa aacactccg tgaaataac accagacaa tagtgttgc ccacctcca ggaagagct aatcgctgt gaagctccg cgaagctga agctaacct ctctttgaa ggatttgcg ccaaagagt gtcagaact tagcaatca gtggcagcg ccacctcca ggagcacaa acaggagcc gactgcctc cggggaacc acacagcac tcccaagaa actcctctt ctgggagaa ccgaaacaa tgagacgca gaagttgga ggacgagct tatgaccac taggatacg agaaatgga gactttgac tgaagtgaa ggagttgag gcttaaggt gatggagct tgagactca ggtgcaggt cagtacgaa tcagttgag acgccagga tgaggaaat taagaagca gagagatga gttggagaa ggctgagtt gagggatcg ggaagctgc ggctcgttt gttggagga gcatcgccg gtattcgga tcttgagag taaaatgca agatgagtt gatgaaggc gaggattag cgatgcgga gaaaacgca agctattgc cgagctcac gcaaaagat cagccagct ggagttgaa gagccagga aattgccac cgaaggcga gcttcgaat ccagtcggt cgacgatag cgatcgagt tagagaatt gcaagataa agtggctga tcttcaagc cgaggtcat gagattgga atcgttcaa gagacgaat aatcgccgg gggtggtcg tctgccgtc ggagaggtc ttgttccat cgacagcac cgacgacga tcccaagat cagattgga cgatccttc cctgagatt aaacttagt cgagtctcc tacctccaa atccgagct tacttttag cgacatgtg tcaaaatcc tactataaa aaacgacaa caaagtaga tctagatcc caacatgaa tacgcacac cactataga tgctcacga agagaagtg tgccccaaa ggtctgaca acacgactg aaattttag gaataagat tttgtagta ttttttgcc tatatatac ttttccaat ggtcaatat aacgggatt tgtcattat agagtacat actacaaac gaataggaa atttggtgc atttaagta agtctaggt cgtcataat ttattgttt attttgtat taatatatt tttagattt atgtactta accgattct agtgtggaa atttcgtgt attttgtta atgtctggt atgtaattt aaaataggg tagaatgtt cttgggaat tcaaccaag agtaactta tgaattagt gagacaact aggagatgt tagattaga ttttctaat attctatct gtgttacaa tggtagtat taaattgag agggctgtt tgtttatac taagaatat tttgctgcg caaagtcgt tgaaacagt atttttctt tagaagtgg acaagttga tgaaccaat caacggtgc tatttttat cagttgcaa taattcaaa aaacattcc aaacgttaa acatcgaaa gttttattt ttagataga aattaataa atactctta gagaaacat attgcacat ttatttgtt ttttatttt tataatatt tccttaact tattaatat tttccctga aggacagca ataaaaatt gatgttgca a
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
