Status
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Gene information
General information
TC002602 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 22125276..22204395
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002602
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03413

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 7 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (41 dpi)
    Days post injection


    Adult morphology
    adult size decreased - Number of animals showing the phenotype: 8


    motility decreased - Number of animals showing the phenotype: 9 (19 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002602 RA
      MPPTAMRLE SPPCNNNNN ASGAELELL AKLEEANRL 
      YESDAKSLN SLSGASGHS RKSSDTSQI SLTSGTSST 
      QDRAEDCGE EDLWTTWGR IVNDWENNW KKQNAQIRE 
      LVRKGIPLH FRAIVWQLL CSAAEAPEK KLYAEYIKT 
      KSPCEKVIR RDIARTYPE HDFFKEKDG LGQESLFNV 
      IKAYSLHDR EVGYCQGSG FIVGLLLMQ MPEEEAFAV 
      LVKIMQDYR MRDMFKPSM AELGLCMYQ LENLVAEYL 
      PDLNQHFQS QNFHTSMYA SSWFLTLFT TALSLPLAC 
      RIMDVFLSE GMEIIFKVA LAMLTLGKD ELMSLDMEG 
      MLKFFQKEL PVKAEADPE NLMQVAYNM KYNAKKMKK 
      LEKEYNVIK TKEQEEMAE LKRLRTENK QLKHRCEML 
      EEESKALAD RLVRGQVSR AEEEETTFV VQRELDALR 
      HTHLETTHQ LALANEKIR SLSLMMEET HTSRQSSID 
      EISLKQEQL QQREEMIEC LQEELVKVR LREAENDAL 
      IRDLRARIH ELEEDKKTL REITPDNSV AHLQEELIA 
      VKLREAEAN LSLKDLRQR VSELSNQWQ RHLQEHKQE 
      PTASGEPHS TPKKLLFWE NRNNETQKL EDELMTTRI 
      REMETLTEV KELRLKVME LETQVQVST NQLRRQDEE 
      IKKQRDELE KAELRDREA AARLLEEHR RYSDLESKM 
      QDELMKARI SDAEKTQAI AELTQKISQ LELKSQEIA 
      TEGELRIQS VDDSDRVRE LQDKVADLQ AEVMRLESF 
      KRRIIAGGG RLPSERSCS IDSTDDDPK IRLDDPSLR 
      LNLVESPTS KSELTFSDM CQNPTIKND NKVDLDPNM 
      NTHTTIDAH EEKCAPKV
    CDS sequence
    • >TC002602 RA
      atgcctccg acagccatg aggctcgag agccctcct 
      tgcaacaat aataacaac gcatctgga gcagaactt 
      gaacttctc gccaaactg gaggaagca aaccgtctt 
      tacgagtcc gacgccaag tccctcaat tcgctgagc 
      ggggcgtcg gggcactcg aggaaaagt tccgacact 
      tcgcaaata tcgctaacc tcgggcacg agctccaca 
      caagatcga gctgaggat tgcggtgag gaggatttg 
      tggaccact tgggggcgt attgtcaat gattgggag 
      aataattgg aagaagcag aatgctcaa atcagggaa 
      ttggtgcgg aagggcatt ccgttacat ttcagggcg 
      atagtttgg caattgctg tgcagtgca gccgaagct 
      cctgaaaag aaactctac gccgaatat atcaagaca 
      aaatctccg tgtgaaaaa gtcatccgt cgagacatc 
      gcccgcact taccccgag cacgacttc ttcaaagaa 
      aaagatggt cttggccaa gaatctctc ttcaacgtc 
      atcaaagcc tactcccta cacgatcgg gaagtgggt 
      tattgccag ggatcgggt tttatcgtc ggactttta 
      cttatgcaa atgccagaa gaggaagct ttcgctgtg 
      ctcgttaaa attatgcaa gattaccgc atgcgtgac 
      atgttcaaa ccgagtatg gcagaattg ggcctttgt 
      atgtaccag ctcgagaac ctggtcgct gaatatttg 
      ccagacctc aaccaacat ttccagtca cagaatttc 
      cacacttcg atgtatgct tccagttgg tttctgaca 
      ctgtttacc accgctttg tctctgccg ctagcgtgc 
      aggatcatg gatgttttt ttatcagag ggaatggag 
      atcattttc aaagtggca cttgccatg ttgacgttg 
      ggcaaagac gaactcatg tcgctcgat atggaggga 
      atgttgaag tttttccaa aaagaactt cccgtcaaa 
      gccgaagct gacccggaa aaccttatg caagtagcc 
      tacaatatg aaatacaat gccaagaaa atgaaaaaa 
      ttggaaaaa gaatacaat gtgataaaa acaaaggaa 
      caagaggaa atggctgaa ttgaaacgc ttgagaacc 
      gaaaacaag cagttgaaa catcgctgc gaaatgttg 
      gaggaagaa agcaaagct ctagctgat cgtttggtc 
      cgcggtcaa gtcagcaga gctgaagaa gaagaaact 
      acttttgta gttcaacgt gaattggac gctttaaga 
      cacactcat ttggaaacg acacaccaa ttagcttta 
      gcgaacgaa aaaattagg tcattgtct ttgatgatg 
      gaagaaact cacacttcg agacaatct tccatcgac 
      gaaatttcc ctcaaacaa gagcaactg caacaaagg 
      gaagaaatg atcgaatgc ctccaagaa gaactggtc 
      aaagtgcga ttacgtgaa gctgaaaac gacgcttta 
      attcgcgac ttaagagcc agaatacac gagttggaa 
      gaagacaaa aaaacactc cgtgaaata acaccagac 
      aatagtgtt gcccacctc caggaagag ctaatcgct 
      gtgaagctc cgcgaagct gaagctaac ctctctttg 
      aaggatttg cgccaaaga gtgtcagaa cttagcaat 
      cagtggcag cgccacctc caggagcac aaacaggag 
      ccgactgcc tccggggaa ccacacagc actcccaag 
      aaactcctc ttctgggag aaccgaaac aatgagacg 
      cagaagttg gaggacgag cttatgacc actaggata 
      cgagaaatg gagactttg actgaagtg aaggagttg 
      aggcttaag gtgatggag cttgagact caggtgcag 
      gtcagtacg aatcagttg agacgccag gatgaggaa 
      attaagaag cagagagat gagttggag aaggctgag 
      ttgagggat cgggaagct gcggctcgt ttgttggag 
      gagcatcgc cggtattcg gatcttgag agtaaaatg 
      caagatgag ttgatgaag gcgaggatt agcgatgcg 
      gagaaaacg caagctatt gccgagctc acgcaaaag 
      atcagccag ctggagttg aagagccag gaaattgcc 
      accgaaggc gagcttcga atccagtcg gtcgacgat 
      agcgatcga gttagagaa ttgcaagat aaagtggct 
      gatcttcaa gccgaggtc atgagattg gaatcgttc 
      aagagacga ataatcgcc gggggtggt cgtctgccg 
      tcggagagg tcttgttcc atcgacagc accgacgac 
      gatcccaag atcagattg gacgatcct tccctgaga 
      ttaaactta gtcgagtct cctacctcc aaatccgag 
      cttactttt agcgacatg tgtcaaaat cctactata 
      aaaaacgac aacaaagta gatctagat cccaacatg 
      aatacgcac accactata gatgctcac gaagagaag 
      tgtgcccca aaggtctga
    mRNA sequence
    • >TC002602 RA
      catacaatt ttgtaatca gctgggcgg tgacagttc 
      gattttgga catttgcgt ggttaggta tcgacgcgt 
      gcgacgcta gacacgtca aatacggcg attactgtt 
      tttatttat ctcttcgtt tttaattta tttggaaag 
      ctttaattt tgtgtgaga actgggaga ttgttaatg 
      tgacgatat tgtaaagtt tgattttat ttgtttcga 
      attcgtcaa gtggttgtt ggtgaaagt gtgggtgtt 
      cttttatgg taaacaaaa acatattct agggttttc 
      ggatcattt tagtggtgg tggatcgtt gagctgtct 
      cgtggtcga gtccgatgg aggctgcta ctcagcaat 
      gcctccgac agccatgag gctcgagag ccctccttg 
      caacaataa taacaacgc atctggagc agaacttga 
      acttctcgc caaactgga ggaagcaaa ccgtcttta 
      cgagtccga cgccaagtc cctcaattc gctgagcgg 
      ggcgtcggg gcactcgag gaaaagttc cgacacttc 
      gcaaatatc gctaacctc gggcacgag ctccacaca 
      agatcgagc tgaggattg cggtgagga ggatttgtg 
      gaccacttg ggggcgtat tgtcaatga ttgggagaa 
      taattggaa gaagcagaa tgctcaaat cagggaatt 
      ggtgcggaa gggcattcc gttacattt cagggcgat 
      agtttggca attgctgtg cagtgcagc cgaagctcc 
      tgaaaagaa actctacgc cgaatatat caagacaaa 
      atctccgtg tgaaaaagt catccgtcg agacatcgc 
      ccgcactta ccccgagca cgacttctt caaagaaaa 
      agatggtct tggccaaga atctctctt caacgtcat 
      caaagccta ctccctaca cgatcggga agtgggtta 
      ttgccaggg atcgggttt tatcgtcgg acttttact 
      tatgcaaat gccagaaga ggaagcttt cgctgtgct 
      cgttaaaat tatgcaaga ttaccgcat gcgtgacat 
      gttcaaacc gagtatggc agaattggg cctttgtat 
      gtaccagct cgagaacct ggtcgctga atatttgcc 
      agacctcaa ccaacattt ccagtcaca gaatttcca 
      cacttcgat gtatgcttc cagttggtt tctgacact 
      gtttaccac cgctttgtc tctgccgct agcgtgcag 
      gatcatgga tgttttttt atcagaggg aatggagat 
      cattttcaa agtggcact tgccatgtt gacgttggg 
      caaagacga actcatgtc gctcgatat ggagggaat 
      gttgaagtt tttccaaaa agaacttcc cgtcaaagc 
      cgaagctga cccggaaaa ccttatgca agtagccta 
      caatatgaa atacaatgc caagaaaat gaaaaaatt 
      ggaaaaaga atacaatgt gataaaaac aaaggaaca 
      agaggaaat ggctgaatt gaaacgctt gagaaccga 
      aaacaagca gttgaaaca tcgctgcga aatgttgga 
      ggaagaaag caaagctct agctgatcg tttggtccg 
      cggtcaagt cagcagagc tgaagaaga agaaactac 
      ttttgtagt tcaacgtga attggacgc tttaagaca 
      cactcattt ggaaacgac acaccaatt agctttagc 
      gaacgaaaa aattaggtc attgtcttt gatgatgga 
      agaaactca cacttcgag acaatcttc catcgacga 
      aatttccct caaacaaga gcaactgca acaaaggga 
      agaaatgat cgaatgcct ccaagaaga actggtcaa 
      agtgcgatt acgtgaagc tgaaaacga cgctttaat 
      tcgcgactt aagagccag aatacacga gttggaaga 
      agacaaaaa aacactccg tgaaataac accagacaa 
      tagtgttgc ccacctcca ggaagagct aatcgctgt 
      gaagctccg cgaagctga agctaacct ctctttgaa 
      ggatttgcg ccaaagagt gtcagaact tagcaatca 
      gtggcagcg ccacctcca ggagcacaa acaggagcc 
      gactgcctc cggggaacc acacagcac tcccaagaa 
      actcctctt ctgggagaa ccgaaacaa tgagacgca 
      gaagttgga ggacgagct tatgaccac taggatacg 
      agaaatgga gactttgac tgaagtgaa ggagttgag 
      gcttaaggt gatggagct tgagactca ggtgcaggt 
      cagtacgaa tcagttgag acgccagga tgaggaaat 
      taagaagca gagagatga gttggagaa ggctgagtt 
      gagggatcg ggaagctgc ggctcgttt gttggagga 
      gcatcgccg gtattcgga tcttgagag taaaatgca 
      agatgagtt gatgaaggc gaggattag cgatgcgga 
      gaaaacgca agctattgc cgagctcac gcaaaagat 
      cagccagct ggagttgaa gagccagga aattgccac 
      cgaaggcga gcttcgaat ccagtcggt cgacgatag 
      cgatcgagt tagagaatt gcaagataa agtggctga 
      tcttcaagc cgaggtcat gagattgga atcgttcaa 
      gagacgaat aatcgccgg gggtggtcg tctgccgtc 
      ggagaggtc ttgttccat cgacagcac cgacgacga 
      tcccaagat cagattgga cgatccttc cctgagatt 
      aaacttagt cgagtctcc tacctccaa atccgagct 
      tacttttag cgacatgtg tcaaaatcc tactataaa 
      aaacgacaa caaagtaga tctagatcc caacatgaa 
      tacgcacac cactataga tgctcacga agagaagtg 
      tgccccaaa ggtctgaca acacgactg aaattttag 
      gaataagat tttgtagta ttttttgcc tatatatac 
      ttttccaat ggtcaatat aacgggatt tgtcattat 
      agagtacat actacaaac gaataggaa atttggtgc 
      atttaagta agtctaggt cgtcataat ttattgttt 
      attttgtat taatatatt tttagattt atgtactta 
      accgattct agtgtggaa atttcgtgt attttgtta 
      atgtctggt atgtaattt aaaataggg tagaatgtt 
      cttgggaat tcaaccaag agtaactta tgaattagt 
      gagacaact aggagatgt tagattaga ttttctaat 
      attctatct gtgttacaa tggtagtat taaattgag 
      agggctgtt tgtttatac taagaatat tttgctgcg 
      caaagtcgt tgaaacagt atttttctt tagaagtgg 
      acaagttga tgaaccaat caacggtgc tatttttat 
      cagttgcaa taattcaaa aaacattcc aaacgttaa 
      acatcgaaa gttttattt ttagataga aattaataa 
      atactctta gagaaacat attgcacat ttatttgtt 
      ttttatttt tataatatt tccttaact tattaatat 
      tttccctga aggacagca ataaaaatt gatgttgca 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY