Status
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Gene information
General information
TC002602 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 22125276..22204395
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002602
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03413

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 7 (22 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 5 (41 dpi)
    Days post injection

    Adult morphology
    adult size decreased - Number of animals showing the phenotype: 8


    motility decreased - Number of animals showing the phenotype: 9 (19 dpi)
    Days post injection

    Fertility
    egg production: number no/almost no eggs -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002602 RA
      MPPTAMRLE SPPCNNNNN ASGAELELL AKLEEANRL 
YESDAKSLN SLSGASGHS RKSSDTSQI SLTSGTSST 
QDRAEDCGE EDLWTTWGR IVNDWENNW KKQNAQIRE 
LVRKGIPLH FRAIVWQLL CSAAEAPEK KLYAEYIKT 
KSPCEKVIR RDIARTYPE HDFFKEKDG LGQESLFNV 
IKAYSLHDR EVGYCQGSG FIVGLLLMQ MPEEEAFAV 
LVKIMQDYR MRDMFKPSM AELGLCMYQ LENLVAEYL 
PDLNQHFQS QNFHTSMYA SSWFLTLFT TALSLPLAC 
RIMDVFLSE GMEIIFKVA LAMLTLGKD ELMSLDMEG 
MLKFFQKEL PVKAEADPE NLMQVAYNM KYNAKKMKK 
LEKEYNVIK TKEQEEMAE LKRLRTENK QLKHRCEML 
EEESKALAD RLVRGQVSR AEEEETTFV VQRELDALR 
HTHLETTHQ LALANEKIR SLSLMMEET HTSRQSSID 
EISLKQEQL QQREEMIEC LQEELVKVR LREAENDAL 
IRDLRARIH ELEEDKKTL REITPDNSV AHLQEELIA 
VKLREAEAN LSLKDLRQR VSELSNQWQ RHLQEHKQE 
PTASGEPHS TPKKLLFWE NRNNETQKL EDELMTTRI 
REMETLTEV KELRLKVME LETQVQVST NQLRRQDEE 
IKKQRDELE KAELRDREA AARLLEEHR RYSDLESKM 
QDELMKARI SDAEKTQAI AELTQKISQ LELKSQEIA 
TEGELRIQS VDDSDRVRE LQDKVADLQ AEVMRLESF 
KRRIIAGGG RLPSERSCS IDSTDDDPK IRLDDPSLR 
LNLVESPTS KSELTFSDM CQNPTIKND NKVDLDPNM 
NTHTTIDAH EEKCAPKV
    CDS sequence
    • >TC002602 RA
      atgcctccg acagccatg aggctcgag agccctcct 
tgcaacaat aataacaac gcatctgga gcagaactt 
gaacttctc gccaaactg gaggaagca aaccgtctt 
tacgagtcc gacgccaag tccctcaat tcgctgagc 
ggggcgtcg gggcactcg aggaaaagt tccgacact 
tcgcaaata tcgctaacc tcgggcacg agctccaca 
caagatcga gctgaggat tgcggtgag gaggatttg 
tggaccact tgggggcgt attgtcaat gattgggag 
aataattgg aagaagcag aatgctcaa atcagggaa 
ttggtgcgg aagggcatt ccgttacat ttcagggcg 
atagtttgg caattgctg tgcagtgca gccgaagct 
cctgaaaag aaactctac gccgaatat atcaagaca 
aaatctccg tgtgaaaaa gtcatccgt cgagacatc 
gcccgcact taccccgag cacgacttc ttcaaagaa 
aaagatggt cttggccaa gaatctctc ttcaacgtc 
atcaaagcc tactcccta cacgatcgg gaagtgggt 
tattgccag ggatcgggt tttatcgtc ggactttta 
cttatgcaa atgccagaa gaggaagct ttcgctgtg 
ctcgttaaa attatgcaa gattaccgc atgcgtgac 
atgttcaaa ccgagtatg gcagaattg ggcctttgt 
atgtaccag ctcgagaac ctggtcgct gaatatttg 
ccagacctc aaccaacat ttccagtca cagaatttc 
cacacttcg atgtatgct tccagttgg tttctgaca 
ctgtttacc accgctttg tctctgccg ctagcgtgc 
aggatcatg gatgttttt ttatcagag ggaatggag 
atcattttc aaagtggca cttgccatg ttgacgttg 
ggcaaagac gaactcatg tcgctcgat atggaggga 
atgttgaag tttttccaa aaagaactt cccgtcaaa 
gccgaagct gacccggaa aaccttatg caagtagcc 
tacaatatg aaatacaat gccaagaaa atgaaaaaa 
ttggaaaaa gaatacaat gtgataaaa acaaaggaa 
caagaggaa atggctgaa ttgaaacgc ttgagaacc 
gaaaacaag cagttgaaa catcgctgc gaaatgttg 
gaggaagaa agcaaagct ctagctgat cgtttggtc 
cgcggtcaa gtcagcaga gctgaagaa gaagaaact 
acttttgta gttcaacgt gaattggac gctttaaga 
cacactcat ttggaaacg acacaccaa ttagcttta 
gcgaacgaa aaaattagg tcattgtct ttgatgatg 
gaagaaact cacacttcg agacaatct tccatcgac 
gaaatttcc ctcaaacaa gagcaactg caacaaagg 
gaagaaatg atcgaatgc ctccaagaa gaactggtc 
aaagtgcga ttacgtgaa gctgaaaac gacgcttta 
attcgcgac ttaagagcc agaatacac gagttggaa 
gaagacaaa aaaacactc cgtgaaata acaccagac 
aatagtgtt gcccacctc caggaagag ctaatcgct 
gtgaagctc cgcgaagct gaagctaac ctctctttg 
aaggatttg cgccaaaga gtgtcagaa cttagcaat 
cagtggcag cgccacctc caggagcac aaacaggag 
ccgactgcc tccggggaa ccacacagc actcccaag 
aaactcctc ttctgggag aaccgaaac aatgagacg 
cagaagttg gaggacgag cttatgacc actaggata 
cgagaaatg gagactttg actgaagtg aaggagttg 
aggcttaag gtgatggag cttgagact caggtgcag 
gtcagtacg aatcagttg agacgccag gatgaggaa 
attaagaag cagagagat gagttggag aaggctgag 
ttgagggat cgggaagct gcggctcgt ttgttggag 
gagcatcgc cggtattcg gatcttgag agtaaaatg 
caagatgag ttgatgaag gcgaggatt agcgatgcg 
gagaaaacg caagctatt gccgagctc acgcaaaag 
atcagccag ctggagttg aagagccag gaaattgcc 
accgaaggc gagcttcga atccagtcg gtcgacgat 
agcgatcga gttagagaa ttgcaagat aaagtggct 
gatcttcaa gccgaggtc atgagattg gaatcgttc 
aagagacga ataatcgcc gggggtggt cgtctgccg 
tcggagagg tcttgttcc atcgacagc accgacgac 
gatcccaag atcagattg gacgatcct tccctgaga 
ttaaactta gtcgagtct cctacctcc aaatccgag 
cttactttt agcgacatg tgtcaaaat cctactata 
aaaaacgac aacaaagta gatctagat cccaacatg 
aatacgcac accactata gatgctcac gaagagaag 
tgtgcccca aaggtctga
    mRNA sequence
    • >TC002602 RA
      catacaatt ttgtaatca gctgggcgg tgacagttc 
gattttgga catttgcgt ggttaggta tcgacgcgt 
gcgacgcta gacacgtca aatacggcg attactgtt 
tttatttat ctcttcgtt tttaattta tttggaaag 
ctttaattt tgtgtgaga actgggaga ttgttaatg 
tgacgatat tgtaaagtt tgattttat ttgtttcga 
attcgtcaa gtggttgtt ggtgaaagt gtgggtgtt 
cttttatgg taaacaaaa acatattct agggttttc 
ggatcattt tagtggtgg tggatcgtt gagctgtct 
cgtggtcga gtccgatgg aggctgcta ctcagcaat 
gcctccgac agccatgag gctcgagag ccctccttg 
caacaataa taacaacgc atctggagc agaacttga 
acttctcgc caaactgga ggaagcaaa ccgtcttta 
cgagtccga cgccaagtc cctcaattc gctgagcgg 
ggcgtcggg gcactcgag gaaaagttc cgacacttc 
gcaaatatc gctaacctc gggcacgag ctccacaca 
agatcgagc tgaggattg cggtgagga ggatttgtg 
gaccacttg ggggcgtat tgtcaatga ttgggagaa 
taattggaa gaagcagaa tgctcaaat cagggaatt 
ggtgcggaa gggcattcc gttacattt cagggcgat 
agtttggca attgctgtg cagtgcagc cgaagctcc 
tgaaaagaa actctacgc cgaatatat caagacaaa 
atctccgtg tgaaaaagt catccgtcg agacatcgc 
ccgcactta ccccgagca cgacttctt caaagaaaa 
agatggtct tggccaaga atctctctt caacgtcat 
caaagccta ctccctaca cgatcggga agtgggtta 
ttgccaggg atcgggttt tatcgtcgg acttttact 
tatgcaaat gccagaaga ggaagcttt cgctgtgct 
cgttaaaat tatgcaaga ttaccgcat gcgtgacat 
gttcaaacc gagtatggc agaattggg cctttgtat 
gtaccagct cgagaacct ggtcgctga atatttgcc 
agacctcaa ccaacattt ccagtcaca gaatttcca 
cacttcgat gtatgcttc cagttggtt tctgacact 
gtttaccac cgctttgtc tctgccgct agcgtgcag 
gatcatgga tgttttttt atcagaggg aatggagat 
cattttcaa agtggcact tgccatgtt gacgttggg 
caaagacga actcatgtc gctcgatat ggagggaat 
gttgaagtt tttccaaaa agaacttcc cgtcaaagc 
cgaagctga cccggaaaa ccttatgca agtagccta 
caatatgaa atacaatgc caagaaaat gaaaaaatt 
ggaaaaaga atacaatgt gataaaaac aaaggaaca 
agaggaaat ggctgaatt gaaacgctt gagaaccga 
aaacaagca gttgaaaca tcgctgcga aatgttgga 
ggaagaaag caaagctct agctgatcg tttggtccg 
cggtcaagt cagcagagc tgaagaaga agaaactac 
ttttgtagt tcaacgtga attggacgc tttaagaca 
cactcattt ggaaacgac acaccaatt agctttagc 
gaacgaaaa aattaggtc attgtcttt gatgatgga 
agaaactca cacttcgag acaatcttc catcgacga 
aatttccct caaacaaga gcaactgca acaaaggga 
agaaatgat cgaatgcct ccaagaaga actggtcaa 
agtgcgatt acgtgaagc tgaaaacga cgctttaat 
tcgcgactt aagagccag aatacacga gttggaaga 
agacaaaaa aacactccg tgaaataac accagacaa 
tagtgttgc ccacctcca ggaagagct aatcgctgt 
gaagctccg cgaagctga agctaacct ctctttgaa 
ggatttgcg ccaaagagt gtcagaact tagcaatca 
gtggcagcg ccacctcca ggagcacaa acaggagcc 
gactgcctc cggggaacc acacagcac tcccaagaa 
actcctctt ctgggagaa ccgaaacaa tgagacgca 
gaagttgga ggacgagct tatgaccac taggatacg 
agaaatgga gactttgac tgaagtgaa ggagttgag 
gcttaaggt gatggagct tgagactca ggtgcaggt 
cagtacgaa tcagttgag acgccagga tgaggaaat 
taagaagca gagagatga gttggagaa ggctgagtt 
gagggatcg ggaagctgc ggctcgttt gttggagga 
gcatcgccg gtattcgga tcttgagag taaaatgca 
agatgagtt gatgaaggc gaggattag cgatgcgga 
gaaaacgca agctattgc cgagctcac gcaaaagat 
cagccagct ggagttgaa gagccagga aattgccac 
cgaaggcga gcttcgaat ccagtcggt cgacgatag 
cgatcgagt tagagaatt gcaagataa agtggctga 
tcttcaagc cgaggtcat gagattgga atcgttcaa 
gagacgaat aatcgccgg gggtggtcg tctgccgtc 
ggagaggtc ttgttccat cgacagcac cgacgacga 
tcccaagat cagattgga cgatccttc cctgagatt 
aaacttagt cgagtctcc tacctccaa atccgagct 
tacttttag cgacatgtg tcaaaatcc tactataaa 
aaacgacaa caaagtaga tctagatcc caacatgaa 
tacgcacac cactataga tgctcacga agagaagtg 
tgccccaaa ggtctgaca acacgactg aaattttag 
gaataagat tttgtagta ttttttgcc tatatatac 
ttttccaat ggtcaatat aacgggatt tgtcattat 
agagtacat actacaaac gaataggaa atttggtgc 
atttaagta agtctaggt cgtcataat ttattgttt 
attttgtat taatatatt tttagattt atgtactta 
accgattct agtgtggaa atttcgtgt attttgtta 
atgtctggt atgtaattt aaaataggg tagaatgtt 
cttgggaat tcaaccaag agtaactta tgaattagt 
gagacaact aggagatgt tagattaga ttttctaat 
attctatct gtgttacaa tggtagtat taaattgag 
agggctgtt tgtttatac taagaatat tttgctgcg 
caaagtcgt tgaaacagt atttttctt tagaagtgg 
acaagttga tgaaccaat caacggtgc tatttttat 
cagttgcaa taattcaaa aaacattcc aaacgttaa 
acatcgaaa gttttattt ttagataga aattaataa 
atactctta gagaaacat attgcacat ttatttgtt 
ttttatttt tataatatt tccttaact tattaatat 
tttccctga aggacagca ataaaaatt gatgttgca 
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