Status
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Gene information
General information
TC032570
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002596
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 22299748..22313295
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032570
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03411

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032570 RA
      MNAYRGRGR GRGYFRGAK TNPPPSMRS PRNPPLNTN 
      QSISHDNNC CNGISIVLE LDLDSTSFL NELKSMWNL 
      LKKSPPVVN KLHQYFHLC ENPYEHTIR LMYNCQEFN 
      SAKSKSLPF FIIEEFKIW LSVHRNKVV HLLTPKLKI 
      DVFKIISKQ NAQNLTKLV VDVYEMAQD GEIFLDIIK 
      CMIERKRYK EACQSAVLF NLQDKFSVE DFLLPLILQ 
      DKLYGIDDF LTVSPRHQV ELVTLLDST LGRTSVRDA 
      LASYVFNLD VPDIKWDKL HAKPLKKLI TRLVKMFKL 
      PTNITPNLN KRRNEGALQ FLLHKRFVE NSFGDESWK 
      EMVQEAIGE DEELQRELV AQVSTYGAV AEALWWAHF 
      YNVDKQHWP YNVRMLEEN PDEERLHQR NILPEEESW 
      GYDEVQNTE PVEYHKFPL PFSSIHLID SEESFERFL 
      DGGLQDVEV VGIDCEWKP NFGSQKNEL ALMQIASRK 
      NVFILDIIS IGTKVPHLW QELGKFLFN NCDILKLGF 
      GFTSDILMI KHSLPELNF TPKQVGFLD LLSLWKLLE 
      KYPKVVLPY EVQGSGPSL GTLVNQCLG RPLDKSDQF 
      SNWEKRPLR NSQLVYAAL DAYCLIEVY DVIKGCCEK 
      AEFPFDETC YNLMTNEKA PKKKAKKPV QKKPKPLQA 
      DEEIAQPPS PHSSQVPAA SIKVVCDTM LQGLGKNLR 
      RCGIDTAIL ENYMDHMEC VRYAQDEQR YILTKGNVF 
      NKLYGYVPL GHCLRVNSD NVDEQLKEF VDYYKVNVT 
      VNDVFSVCQ SCNGRSFIK VSRSTMLAL TQSQNSLQY 
      VPPDYDNDI DEATGFSSD DDFDFEPGP PVQTTRKWD 
      LYSDEKLDV GLCQTRLGA KIQVATIPD GVLEKTELF 
      YVCEHCGKI FWDGSHLER VLTGRLQGI VQ
    CDS sequence
    • >TC032570 RA
      atgaacgcc tacaggggg agaggaaga ggcaggggt 
      tatttccgg ggtgcgaaa accaaccca ccacctagc 
      atgagaagc ccccggaac ccaccattg aacacaaac 
      cagagcata tcacatgac aataattgt tgcaatgga 
      ataagcatc gttttagag ttagatctc gacagtacc 
      agtttttta aatgaattg aaaagcatg tggaatttg 
      ttgaagaaa agtccgcca gttgtaaac aaattgcac 
      caatatttt catctatgt gaaaatccg tatgaacac 
      acgataaga ttaatgtac aattgtcag gaatttaac 
      agcgcaaag agcaagtct ttgccgttt tttattata 
      gaagagttt aaaatttgg ttgtctgta catcggaat 
      aaagttgta catctttta acgcctaag ttgaaaatt 
      gacgtgttt aaaattatc tcaaaacag aatgcacaa 
      aatctgacg aagcttgta gtggatgtg tatgagatg 
      gctcaagat ggggaaata tttctggac ataatcaag 
      tgtatgatt gaacgaaaa aggtacaaa gaggcttgc 
      caaagcgct gtgctattt aatttacaa gacaaattt 
      tctgtggaa gattttctg cttcctctc attttgcaa 
      gataaatta tacggcatc gatgatttc ttgaccgtt 
      agtccccga catcaagtt gaattggtc acactttta 
      gattctacc ttaggtagg acgtcagtt agggatgcc 
      ttagcgtcg tatgtgttc aatttagat gttcctgat 
      atcaaatgg gataaactg catgcaaaa cccttgaaa 
      aagttaata actagactt gttaaaatg tttaagctt 
      ccaacaaac ataactcca aacttaaat aaacgaaga 
      aacgaaggt gctttacag tttttgttg cacaagagg 
      tttgtcgaa aattcgttc ggtgacgaa agttggaaa 
      gagatggtt caggaggct atcggagaa gatgaagaa 
      ttacaaagg gagcttgta gcccaagtt agcacctat 
      ggggcagtt gctgaggct ttatggtgg gctcatttt 
      tacaatgtt gataagcaa cattggcct tataatgtg 
      aggatgctt gaagaaaac ccagatgag gaaaggttg 
      catcaaaga aatatatta cctgaggag gagtcgtgg 
      ggttacgac gaagtacaa aataccgaa cctgtggaa 
      tatcataaa tttccgtta cctttctct agtattcat 
      ttgatagat tctgaagaa tcatttgag cgattttta 
      gacggtgga ctacaagac gtcgaagta gtaggaatt 
      gactgtgaa tggaaacca aattttggt agccaaaaa 
      aatgagtta gctttaatg caaatagca tctagaaaa 
      aacgtgttc attcttgat ataatcagt ataggaacc 
      aaagtgccg catttatgg caagaactc ggcaaattc 
      ctttttaat aattgtgat attttaaaa ttaggcttt 
      gggttcacc agtgatatc ttaatgatt aaacattct 
      ttacctgaa cttaatttc actccgaaa caagttgga 
      ttcttagat ttgttgtca ctttggaag ctgctggag 
      aagtacccc aaagttgtg ttgccatat gaagtacaa 
      ggtagtggg cccagtttg ggcacttta gtgaatcaa 
      tgtttgggc cgtcctttg gacaagtct gaccaattc 
      tcaaactgg gaaaaacgc cctttgagg aacagtcag 
      ttagtctat gctgcactt gacgcttat tgcctgatt 
      gaagtgtac gatgttatc aagggttgt tgcgaaaaa 
      gctgagttt cccttcgac gaaacgtgt tataatcta 
      atgacaaac gagaaggcg ccgaaaaag aaggccaag 
      aaaccggtc caaaaaaaa ccaaagcct cttcaagct 
      gatgaagag atagcgcaa cctccatct cctcactca 
      agtcaagtg ccagctgcg tcgattaaa gttgtctgt 
      gatacaatg ttacaaggt ttaggtaag aatctgcga 
      aggtgtgga attgatact gccattctt gaaaattat 
      atggaccat atggagtgt gttagatat gctcaagat 
      gaacaacga tatattttg acgaaggga aatgttttt 
      aataaatta tacggatat gtgccacta ggacattgt 
      cttcgagtc aacagtgat aacgtcgac gaacagctc 
      aaggagttc gttgattat tataaggtc aacgtgaca 
      gtgaatgat gtgttcagc gtgtgtcag tcgtgcaat 
      ggccgaagc tttattaaa gtatcacgg tcaacgatg 
      ttggcctta acacaatcg caaaattcc ctacaatat 
      gtcccccct gactacgat aacgacata gacgaagca 
      actggtttt tcaagtgat gatgacttc gattttgaa 
      cctggacca ccagttcag acaacaaga aaatgggat 
      ctttattcg gatgaaaag ctcgatgtt gggttatgt 
      cagactcga cttggcgca aaaatacaa gtagcaacg 
      atccctgac ggtgtttta gaaaaaacg gaattattt 
      tatgtgtgt gaacattgc ggaaaaatt ttctgggac 
      ggcagtcac ttggaaagg gttctaacc ggaagactt 
      caaggaatt gtgcaatga
    mRNA sequence
    • >TC032570 RA
      cgtccctaa gcaagaatt cgaaatatt tcctgtttg 
      atccaccgg gaatattga catgtctcc tccagttgt 
      acaaattta ttcgtttta aacccggaa gtggattaa 
      agtaatgca ggaaagtgg atgttaata gggacagtt 
      tgtacttac cggtatctt atcgcttat ctacaaatc 
      agcttacct gagcatttt gtaacattt gacgttgac 
      agcgcgaga atgaacgcc tacaggggg agaggaaga 
      ggcaggggt tatttccgg ggtgcgaaa accaaccca 
      ccacctagc atgagaagc ccccggaac ccaccattg 
      aacacaaac cagagcata tcacatgac aataattgt 
      tgcaatgga ataagcatc gttttagag ttagatctc 
      gacagtacc agtttttta aatgaattg aaaagcatg 
      tggaatttg ttgaagaaa agtccgcca gttgtaaac 
      aaattgcac caatatttt catctatgt gaaaatccg 
      tatgaacac acgataaga ttaatgtac aattgtcag 
      gaatttaac agcgcaaag agcaagtct ttgccgttt 
      tttattata gaagagttt aaaatttgg ttgtctgta 
      catcggaat aaagttgta catctttta acgcctaag 
      ttgaaaatt gacgtgttt aaaattatc tcaaaacag 
      aatgcacaa aatctgacg aagcttgta gtggatgtg 
      tatgagatg gctcaagat ggggaaata tttctggac 
      ataatcaag tgtatgatt gaacgaaaa aggtacaaa 
      gaggcttgc caaagcgct gtgctattt aatttacaa 
      gacaaattt tctgtggaa gattttctg cttcctctc 
      attttgcaa gataaatta tacggcatc gatgatttc 
      ttgaccgtt agtccccga catcaagtt gaattggtc 
      acactttta gattctacc ttaggtagg acgtcagtt 
      agggatgcc ttagcgtcg tatgtgttc aatttagat 
      gttcctgat atcaaatgg gataaactg catgcaaaa 
      cccttgaaa aagttaata actagactt gttaaaatg 
      tttaagctt ccaacaaac ataactcca aacttaaat 
      aaacgaaga aacgaaggt gctttacag tttttgttg 
      cacaagagg tttgtcgaa aattcgttc ggtgacgaa 
      agttggaaa gagatggtt caggaggct atcggagaa 
      gatgaagaa ttacaaagg gagcttgta gcccaagtt 
      agcacctat ggggcagtt gctgaggct ttatggtgg 
      gctcatttt tacaatgtt gataagcaa cattggcct 
      tataatgtg aggatgctt gaagaaaac ccagatgag 
      gaaaggttg catcaaaga aatatatta cctgaggag 
      gagtcgtgg ggttacgac gaagtacaa aataccgaa 
      cctgtggaa tatcataaa tttccgtta cctttctct 
      agtattcat ttgatagat tctgaagaa tcatttgag 
      cgattttta gacggtgga ctacaagac gtcgaagta 
      gtaggaatt gactgtgaa tggaaacca aattttggt 
      agccaaaaa aatgagtta gctttaatg caaatagca 
      tctagaaaa aacgtgttc attcttgat ataatcagt 
      ataggaacc aaagtgccg catttatgg caagaactc 
      ggcaaattc ctttttaat aattgtgat attttaaaa 
      ttaggcttt gggttcacc agtgatatc ttaatgatt 
      aaacattct ttacctgaa cttaatttc actccgaaa 
      caagttgga ttcttagat ttgttgtca ctttggaag 
      ctgctggag aagtacccc aaagttgtg ttgccatat 
      gaagtacaa ggtagtggg cccagtttg ggcacttta 
      gtgaatcaa tgtttgggc cgtcctttg gacaagtct 
      gaccaattc tcaaactgg gaaaaacgc cctttgagg 
      aacagtcag ttagtctat gctgcactt gacgcttat 
      tgcctgatt gaagtgtac gatgttatc aagggttgt 
      tgcgaaaaa gctgagttt cccttcgac gaaacgtgt 
      tataatcta atgacaaac gagaaggcg ccgaaaaag 
      aaggccaag aaaccggtc caaaaaaaa ccaaagcct 
      cttcaagct gatgaagag atagcgcaa cctccatct 
      cctcactca agtcaagtg ccagctgcg tcgattaaa 
      gttgtctgt gatacaatg ttacaaggt ttaggtaag 
      aatctgcga aggtgtgga attgatact gccattctt 
      gaaaattat atggaccat atggagtgt gttagatat 
      gctcaagat gaacaacga tatattttg acgaaggga 
      aatgttttt aataaatta tacggatat gtgccacta 
      ggacattgt cttcgagtc aacagtgat aacgtcgac 
      gaacagctc aaggagttc gttgattat tataaggtc 
      aacgtgaca gtgaatgat gtgttcagc gtgtgtcag 
      tcgtgcaat ggccgaagc tttattaaa gtatcacgg 
      tcaacgatg ttggcctta acacaatcg caaaattcc 
      ctacaatat gtcccccct gactacgat aacgacata 
      gacgaagca actggtttt tcaagtgat gatgacttc 
      gattttgaa cctggacca ccagttcag acaacaaga 
      aaatgggat ctttattcg gatgaaaag ctcgatgtt 
      gggttatgt cagactcga cttggcgca aaaatacaa 
      gtagcaacg atccctgac ggtgtttta gaaaaaacg 
      gaattattt tatgtgtgt gaacattgc ggaaaaatt 
      ttctgggac ggcagtcac ttggaaagg gttctaacc 
      ggaagactt caaggaatt gtgcaatga atgcgtttt 
      taaatctat cttatggtg gtattttat tacttaaat 
      cagcttagt taaatgtca tttcagttt tctaaccat 
      tatggaata aaattataa tatttttat tgagtaagg 
      taacacgac ctgtcttat ttgcatgtg gagtagtac 
      aaaagtgat ctgtaaata tgggacaga taatttttt 
      aaggtgccg ttgtagctt cgtggaaag tatgcaaat 
      accagttca ttttttaat tgtaaatat ggaaaattg 
      cttacctaa taagttagg tacaatgtt tttagtaac 
      gaataattt aattttttt tataattag gttttttgc 
      tgttgggtt gctagggaa gtctctaga cattgttgc 
      aaaaagtta tgagtatta ttgatacat attagctgt 
      ttcaaaact ataaaaacg ccaacgttg cattttacg 
      gaattattt ttttaacta agattgata aaaatgtaa 
      aagttaatg attaaatct ctcattgaa agtataaat 
      tacattagc tattatgct tttgaagtg cacgaataa 
      tttataaca tctaatttt gggagaaaa tgcgacgtt 
      ggcattttt atagttttg aaacagcta atagtaatg 
      atataattt ttttactat ttccaatta gagtaacta 
      aaaccggta tttacatat ttatctact tagtttttt 
      tcttatgtg ttgatgcga ttcgtcttt atatattat 
      taaaaaata aagtaaact tgtttttaa tttgtcttt 
      gtgaccttt gtcccttat attcttgcg gatatggcg 
      cataattgt gtaaacgcc gctttagca gtgttttca 
      cggtctgtc aaaaaagta acctccaaa atgtcataa 
      cataacctt caacctcag tgaaaaaat gtcgcacgt 
      acaaaatac aaaaataga aatatttaa acaaatttg 
      ttaatttcg agtgtcaat tggaagcgt agtattcaa 
      tttgagtga tccagagac actttaatc aacattgtg 
      tgcgagaaa tttcaccaa gacgtaaaa atggtcgtg 
      gcctgtcgt actcaacag ttgataaaa acgggcaaa 
      actgtgact atttcaaag aagttcaat tattcgact 
      agtacatcg ctgggattt agaaattat gtagataca 
      gtatcagtg cctcgaagt gataaaccc agagtaagg 
      ctctatgac tgcaaaaga ttccactct tccagggag 
      agcagaagt acgttagcc aaacttaca ccacaagag 
      gtaattgta atcttctga tatttactt aattata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY