| General information | |||
| TC032570
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 22299748..22313295 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032570 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03411 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
remarks: muscle pattern potentially obscured by segmentation defect,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC032570 RA
MNAYRGRGR GRGYFRGAK TNPPPSMRS PRNPPLNTN QSISHDNNC CNGISIVLE LDLDSTSFL NELKSMWNL LKKSPPVVN KLHQYFHLC ENPYEHTIR LMYNCQEFN SAKSKSLPF FIIEEFKIW LSVHRNKVV HLLTPKLKI DVFKIISKQ NAQNLTKLV VDVYEMAQD GEIFLDIIK CMIERKRYK EACQSAVLF NLQDKFSVE DFLLPLILQ DKLYGIDDF LTVSPRHQV ELVTLLDST LGRTSVRDA LASYVFNLD VPDIKWDKL HAKPLKKLI TRLVKMFKL PTNITPNLN KRRNEGALQ FLLHKRFVE NSFGDESWK EMVQEAIGE DEELQRELV AQVSTYGAV AEALWWAHF YNVDKQHWP YNVRMLEEN PDEERLHQR NILPEEESW GYDEVQNTE PVEYHKFPL PFSSIHLID SEESFERFL DGGLQDVEV VGIDCEWKP NFGSQKNEL ALMQIASRK NVFILDIIS IGTKVPHLW QELGKFLFN NCDILKLGF GFTSDILMI KHSLPELNF TPKQVGFLD LLSLWKLLE KYPKVVLPY EVQGSGPSL GTLVNQCLG RPLDKSDQF SNWEKRPLR NSQLVYAAL DAYCLIEVY DVIKGCCEK AEFPFDETC YNLMTNEKA PKKKAKKPV QKKPKPLQA DEEIAQPPS PHSSQVPAA SIKVVCDTM LQGLGKNLR RCGIDTAIL ENYMDHMEC VRYAQDEQR YILTKGNVF NKLYGYVPL GHCLRVNSD NVDEQLKEF VDYYKVNVT VNDVFSVCQ SCNGRSFIK VSRSTMLAL TQSQNSLQY VPPDYDNDI DEATGFSSD DDFDFEPGP PVQTTRKWD LYSDEKLDV GLCQTRLGA KIQVATIPD GVLEKTELF YVCEHCGKI FWDGSHLER VLTGRLQGI VQ
- >TC032570 RA
atgaacgcc tacaggggg agaggaaga ggcaggggt tatttccgg ggtgcgaaa accaaccca ccacctagc atgagaagc ccccggaac ccaccattg aacacaaac cagagcata tcacatgac aataattgt tgcaatgga ataagcatc gttttagag ttagatctc gacagtacc agtttttta aatgaattg aaaagcatg tggaatttg ttgaagaaa agtccgcca gttgtaaac aaattgcac caatatttt catctatgt gaaaatccg tatgaacac acgataaga ttaatgtac aattgtcag gaatttaac agcgcaaag agcaagtct ttgccgttt tttattata gaagagttt aaaatttgg ttgtctgta catcggaat aaagttgta catctttta acgcctaag ttgaaaatt gacgtgttt aaaattatc tcaaaacag aatgcacaa aatctgacg aagcttgta gtggatgtg tatgagatg gctcaagat ggggaaata tttctggac ataatcaag tgtatgatt gaacgaaaa aggtacaaa gaggcttgc caaagcgct gtgctattt aatttacaa gacaaattt tctgtggaa gattttctg cttcctctc attttgcaa gataaatta tacggcatc gatgatttc ttgaccgtt agtccccga catcaagtt gaattggtc acactttta gattctacc ttaggtagg acgtcagtt agggatgcc ttagcgtcg tatgtgttc aatttagat gttcctgat atcaaatgg gataaactg catgcaaaa cccttgaaa aagttaata actagactt gttaaaatg tttaagctt ccaacaaac ataactcca aacttaaat aaacgaaga aacgaaggt gctttacag tttttgttg cacaagagg tttgtcgaa aattcgttc ggtgacgaa agttggaaa gagatggtt caggaggct atcggagaa gatgaagaa ttacaaagg gagcttgta gcccaagtt agcacctat ggggcagtt gctgaggct ttatggtgg gctcatttt tacaatgtt gataagcaa cattggcct tataatgtg aggatgctt gaagaaaac ccagatgag gaaaggttg catcaaaga aatatatta cctgaggag gagtcgtgg ggttacgac gaagtacaa aataccgaa cctgtggaa tatcataaa tttccgtta cctttctct agtattcat ttgatagat tctgaagaa tcatttgag cgattttta gacggtgga ctacaagac gtcgaagta gtaggaatt gactgtgaa tggaaacca aattttggt agccaaaaa aatgagtta gctttaatg caaatagca tctagaaaa aacgtgttc attcttgat ataatcagt ataggaacc aaagtgccg catttatgg caagaactc ggcaaattc ctttttaat aattgtgat attttaaaa ttaggcttt gggttcacc agtgatatc ttaatgatt aaacattct ttacctgaa cttaatttc actccgaaa caagttgga ttcttagat ttgttgtca ctttggaag ctgctggag aagtacccc aaagttgtg ttgccatat gaagtacaa ggtagtggg cccagtttg ggcacttta gtgaatcaa tgtttgggc cgtcctttg gacaagtct gaccaattc tcaaactgg gaaaaacgc cctttgagg aacagtcag ttagtctat gctgcactt gacgcttat tgcctgatt gaagtgtac gatgttatc aagggttgt tgcgaaaaa gctgagttt cccttcgac gaaacgtgt tataatcta atgacaaac gagaaggcg ccgaaaaag aaggccaag aaaccggtc caaaaaaaa ccaaagcct cttcaagct gatgaagag atagcgcaa cctccatct cctcactca agtcaagtg ccagctgcg tcgattaaa gttgtctgt gatacaatg ttacaaggt ttaggtaag aatctgcga aggtgtgga attgatact gccattctt gaaaattat atggaccat atggagtgt gttagatat gctcaagat gaacaacga tatattttg acgaaggga aatgttttt aataaatta tacggatat gtgccacta ggacattgt cttcgagtc aacagtgat aacgtcgac gaacagctc aaggagttc gttgattat tataaggtc aacgtgaca gtgaatgat gtgttcagc gtgtgtcag tcgtgcaat ggccgaagc tttattaaa gtatcacgg tcaacgatg ttggcctta acacaatcg caaaattcc ctacaatat gtcccccct gactacgat aacgacata gacgaagca actggtttt tcaagtgat gatgacttc gattttgaa cctggacca ccagttcag acaacaaga aaatgggat ctttattcg gatgaaaag ctcgatgtt gggttatgt cagactcga cttggcgca aaaatacaa gtagcaacg atccctgac ggtgtttta gaaaaaacg gaattattt tatgtgtgt gaacattgc ggaaaaatt ttctgggac ggcagtcac ttggaaagg gttctaacc ggaagactt caaggaatt gtgcaatga
- >TC032570 RA
cgtccctaa gcaagaatt cgaaatatt tcctgtttg atccaccgg gaatattga catgtctcc tccagttgt acaaattta ttcgtttta aacccggaa gtggattaa agtaatgca ggaaagtgg atgttaata gggacagtt tgtacttac cggtatctt atcgcttat ctacaaatc agcttacct gagcatttt gtaacattt gacgttgac agcgcgaga atgaacgcc tacaggggg agaggaaga ggcaggggt tatttccgg ggtgcgaaa accaaccca ccacctagc atgagaagc ccccggaac ccaccattg aacacaaac cagagcata tcacatgac aataattgt tgcaatgga ataagcatc gttttagag ttagatctc gacagtacc agtttttta aatgaattg aaaagcatg tggaatttg ttgaagaaa agtccgcca gttgtaaac aaattgcac caatatttt catctatgt gaaaatccg tatgaacac acgataaga ttaatgtac aattgtcag gaatttaac agcgcaaag agcaagtct ttgccgttt tttattata gaagagttt aaaatttgg ttgtctgta catcggaat aaagttgta catctttta acgcctaag ttgaaaatt gacgtgttt aaaattatc tcaaaacag aatgcacaa aatctgacg aagcttgta gtggatgtg tatgagatg gctcaagat ggggaaata tttctggac ataatcaag tgtatgatt gaacgaaaa aggtacaaa gaggcttgc caaagcgct gtgctattt aatttacaa gacaaattt tctgtggaa gattttctg cttcctctc attttgcaa gataaatta tacggcatc gatgatttc ttgaccgtt agtccccga catcaagtt gaattggtc acactttta gattctacc ttaggtagg acgtcagtt agggatgcc ttagcgtcg tatgtgttc aatttagat gttcctgat atcaaatgg gataaactg catgcaaaa cccttgaaa aagttaata actagactt gttaaaatg tttaagctt ccaacaaac ataactcca aacttaaat aaacgaaga aacgaaggt gctttacag tttttgttg cacaagagg tttgtcgaa aattcgttc ggtgacgaa agttggaaa gagatggtt caggaggct atcggagaa gatgaagaa ttacaaagg gagcttgta gcccaagtt agcacctat ggggcagtt gctgaggct ttatggtgg gctcatttt tacaatgtt gataagcaa cattggcct tataatgtg aggatgctt gaagaaaac ccagatgag gaaaggttg catcaaaga aatatatta cctgaggag gagtcgtgg ggttacgac gaagtacaa aataccgaa cctgtggaa tatcataaa tttccgtta cctttctct agtattcat ttgatagat tctgaagaa tcatttgag cgattttta gacggtgga ctacaagac gtcgaagta gtaggaatt gactgtgaa tggaaacca aattttggt agccaaaaa aatgagtta gctttaatg caaatagca tctagaaaa aacgtgttc attcttgat ataatcagt ataggaacc aaagtgccg catttatgg caagaactc ggcaaattc ctttttaat aattgtgat attttaaaa ttaggcttt gggttcacc agtgatatc ttaatgatt aaacattct ttacctgaa cttaatttc actccgaaa caagttgga ttcttagat ttgttgtca ctttggaag ctgctggag aagtacccc aaagttgtg ttgccatat gaagtacaa ggtagtggg cccagtttg ggcacttta gtgaatcaa tgtttgggc cgtcctttg gacaagtct gaccaattc tcaaactgg gaaaaacgc cctttgagg aacagtcag ttagtctat gctgcactt gacgcttat tgcctgatt gaagtgtac gatgttatc aagggttgt tgcgaaaaa gctgagttt cccttcgac gaaacgtgt tataatcta atgacaaac gagaaggcg ccgaaaaag aaggccaag aaaccggtc caaaaaaaa ccaaagcct cttcaagct gatgaagag atagcgcaa cctccatct cctcactca agtcaagtg ccagctgcg tcgattaaa gttgtctgt gatacaatg ttacaaggt ttaggtaag aatctgcga aggtgtgga attgatact gccattctt gaaaattat atggaccat atggagtgt gttagatat gctcaagat gaacaacga tatattttg acgaaggga aatgttttt aataaatta tacggatat gtgccacta ggacattgt cttcgagtc aacagtgat aacgtcgac gaacagctc aaggagttc gttgattat tataaggtc aacgtgaca gtgaatgat gtgttcagc gtgtgtcag tcgtgcaat ggccgaagc tttattaaa gtatcacgg tcaacgatg ttggcctta acacaatcg caaaattcc ctacaatat gtcccccct gactacgat aacgacata gacgaagca actggtttt tcaagtgat gatgacttc gattttgaa cctggacca ccagttcag acaacaaga aaatgggat ctttattcg gatgaaaag ctcgatgtt gggttatgt cagactcga cttggcgca aaaatacaa gtagcaacg atccctgac ggtgtttta gaaaaaacg gaattattt tatgtgtgt gaacattgc ggaaaaatt ttctgggac ggcagtcac ttggaaagg gttctaacc ggaagactt caaggaatt gtgcaatga atgcgtttt taaatctat cttatggtg gtattttat tacttaaat cagcttagt taaatgtca tttcagttt tctaaccat tatggaata aaattataa tatttttat tgagtaagg taacacgac ctgtcttat ttgcatgtg gagtagtac aaaagtgat ctgtaaata tgggacaga taatttttt aaggtgccg ttgtagctt cgtggaaag tatgcaaat accagttca ttttttaat tgtaaatat ggaaaattg cttacctaa taagttagg tacaatgtt tttagtaac gaataattt aattttttt tataattag gttttttgc tgttgggtt gctagggaa gtctctaga cattgttgc aaaaagtta tgagtatta ttgatacat attagctgt ttcaaaact ataaaaacg ccaacgttg cattttacg gaattattt ttttaacta agattgata aaaatgtaa aagttaatg attaaatct ctcattgaa agtataaat tacattagc tattatgct tttgaagtg cacgaataa tttataaca tctaatttt gggagaaaa tgcgacgtt ggcattttt atagttttg aaacagcta atagtaatg atataattt ttttactat ttccaatta gagtaacta aaaccggta tttacatat ttatctact tagtttttt tcttatgtg ttgatgcga ttcgtcttt atatattat taaaaaata aagtaaact tgtttttaa tttgtcttt gtgaccttt gtcccttat attcttgcg gatatggcg cataattgt gtaaacgcc gctttagca gtgttttca cggtctgtc aaaaaagta acctccaaa atgtcataa cataacctt caacctcag tgaaaaaat gtcgcacgt acaaaatac aaaaataga aatatttaa acaaatttg ttaatttcg agtgtcaat tggaagcgt agtattcaa tttgagtga tccagagac actttaatc aacattgtg tgcgagaaa tttcaccaa gacgtaaaa atggtcgtg gcctgtcgt actcaacag ttgataaaa acgggcaaa actgtgact atttcaaag aagttcaat tattcgact agtacatcg ctgggattt agaaattat gtagataca gtatcagtg cctcgaagt gataaaccc agagtaagg ctctatgac tgcaaaaga ttccactct tccagggag agcagaagt acgttagcc aaacttaca ccacaagag gtaattgta atcttctga tatttactt aattata
