Status
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Gene information
General information
TC002584 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 22806346..22819122
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002584
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03409

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002584 RA
      MDTYPDVAE HESSAKRIK LDLRESNIG ELCNGGDFS 
      SYSTSVGGP IDIPEGISP DCDSGYEAS TLESIPTSS 
      HQTSPHTDP GDVPESLSS LSEIFLTPS RTDQADTSQ 
      DSIEADDDN ASTVSEISG LSDLSGQDW KPMAGSMIW 
      IQKQMQNGV NPRTLLSDL GVDLDQVPQ YVDEITLWK 
      LIINMLAEP PRRNKLRHV NTLDDVVRL VKGAQNIIV 
      LTGAGVSVS CGIPDFRSR DGIYVRLAI DFPDLPDPQ 
      AMFDISYFS QDPRPFFKF ARDIYPGKF TPSPCHRFI 
      KMLENYGKL LRNYTQNID TLEKVANIE KVIECHGSF 
      ATATCTKCG HKVTADAIR EIVLAQQIP LCEKCHPGK 
      TSVPCIEEY KENSEEIDY RQLVSSGIM KPDIVFFGE 
      GLPDTFHEA MAQDKTECD LLLVIGSSL KVRPVALIP 
      SSLPPHVPQ ILINREPLP HCHFDVELL GDCDVIVNH 
      LCHLFGGVW KEGVFDDNM LEEASHLLP LQEPSFDMT 
      PPCVKSQTE PNCTDDNLE KASPCLCST KCSSSSTVE 
      KDEELTLEC SCNKQTSII NKQSLLDSD LVNSLDLKS 
      VKERHMSVD SARDSGIGD NSNFTDLET KYDDTSDEN 
      PDLGEYNTN TNTASDPQF TNISNDEAN KTNTYLEST 
      TNTSDLKGF WQPKIKKSL AERLPPKSF YLVKPSRYI 
      FPGAEIYYD PDEKFGYYE GSSSSNNSD SESENGEPV 
      SN
    CDS sequence
    • >TC002584 RA
      atggatacc tatcctgac gttgctgaa cacgaatcc 
      agtgcgaaa cgaattaaa ctcgaccta agagagtcg 
      aatataggt gaattgtgc aatgggggc gatttttct 
      agttattcc acatcagtt ggcggcccg attgacatc 
      cccgaagga atatcacct gattgcgat tccggttat 
      gaagcatcc acgttagaa tcaattcca acttcttca 
      caccagacc tcacctcac actgacccc ggcgatgtc 
      ccagagagt ttatccagt ctttccgaa atatttcta 
      acaccatcc cgaacagat caagcggac acatctcag 
      gacagtatc gaagccgac gatgacaat gcctccaca 
      gttagcgaa ataagcggt ctttcggac ctctctgga 
      caagattgg aaaccaatg gctggcagc atgatttgg 
      attcaaaaa cagatgcaa aacggcgtg aatccccga 
      acgctgctg agcgatctg ggtgtggat ctggatcaa 
      gtgccgcaa tatgtagac gaaataact ctctggaaa 
      ttaattata aacatgttg gccgagccg ccacgacgg 
      aacaaattg cgacatgtt aatactttg gacgatgta 
      gttcgattg gttaaagga gcacaaaat ataatagtt 
      ttaaccggc gctggagtc tctgtttcg tgtggaatt 
      ccagacttt cgatcacgt gatgggatt tacgttcga 
      ctggcgata gattttcca gatcttcca gacccgcaa 
      gcaatgttt gatatcagc tatttcagt caagaccct 
      cggcccttc ttcaaattc gcacgagat atataccca 
      ggaaaattc acgccaagt ccttgtcac agatttata 
      aaaatgttg gaaaactac ggcaaactc ttgcgtaac 
      tacacccaa aacattgac acactcgaa aaagttgca 
      aacatagaa aaagtgatc gaatgtcac ggttcattc 
      gcaaccgcc acttgcaca aaatgtgga cacaaagtc 
      accgctgat gctatcaga gagatagtt ttagcgcag 
      caaattcca ctttgtgaa aaatgtcat ccgggaaaa 
      acttccgtt ccttgtata gaagaatat aaagaaaac 
      agtgaggaa atagattat agacaactt gtgtcttcg 
      ggaatcatg aaaccagac attgtgttc tttggcgag 
      ggacttcca gatacattc cacgaagcg atggctcaa 
      gacaagaca gagtgcgat ttgcttttg gttattgga 
      agttcgttg aaggtgcga cctgttgct ctgattccc 
      agctcgttg cctccgcat gttccacaa attctgatc 
      aatcgggag cctttaccg cattgccac ttcgatgtg 
      gagctgttg ggagattgc gacgtcatc gtcaaccac 
      ttgtgccat ttgtttggc ggtgtttgg aaggaaggc 
      gttttcgac gacaacatg ttggaagaa gcctcgcat 
      ttgcttcca ttgcaagag ccgagtttc gacatgact 
      cctccttgt gttaaaagt caaaccgag ccaaattgt 
      acagatgat aacttagag aaagctagc ccgtgttta 
      tgcagtaca aaatgtagt agttcctca acagtagaa 
      aaagacgaa gagttaacc cttgaatgt agttgcaac 
      aaacaaacg tcaattatt aataagcaa tccttgctg 
      gattcggac cttgtcaat tcattagat ttaaaatcg 
      gtcaaagaa cgccacatg tcagtggat tctgcgcgg 
      gattccggc atcggagac aattcgaat ttcaccgat 
      ttggagacg aaatacgac gacacatcg gatgaaaac 
      ccggatttg ggcgaatac aacacaaac actaacact 
      gctagtgat cctcaattt acaaacata tcgaatgat 
      gaagcgaac aagacgaat acatatttg gaaagtaca 
      acaaacact tcagatctg aaaggtttc tggcaaccg 
      aaaatcaag aaaagcctt gcggaacga ttgccaccg 
      aaatcgttc tatcttgtc aaaccgagc cgatatatt 
      tttccaggc gctgagatt tattacgac cctgatgaa 
      aagtttggt tattatgag ggttcatcg agttcgaac 
      aactctgac agcgagtcc gaaaacggt gaaccagtc 
      agtaattag
    mRNA sequence
    • >TC002584 RA
      ttcgtgtca aagatggat acctatcct gacgttgct 
      gaacacgaa tccagtgcg aaacgaatt aaactcgac 
      ctaagagag tcgaatata ggtgaattg tgcaatggg 
      ggcgatttt tctagttat tccacatca gttggcggc 
      ccgattgac atccccgaa ggaatatca cctgattgc 
      gattccggt tatgaagca tccacgtta gaatcaatt 
      ccaacttct tcacaccag acctcacct cacactgac 
      cccggcgat gtcccagag agtttatcc agtctttcc 
      gaaatattt ctaacacca tcccgaaca gatcaagcg 
      gacacatct caggacagt atcgaagcc gacgatgac 
      aatgcctcc acagttagc gaaataagc ggtctttcg 
      gacctctct ggacaagat tggaaacca atggctggc 
      agcatgatt tggattcaa aaacagatg caaaacggc 
      gtgaatccc cgaacgctg ctgagcgat ctgggtgtg 
      gatctggat caagtgccg caatatgta gacgaaata 
      actctctgg aaattaatt ataaacatg ttggccgag 
      ccgccacga cggaacaaa ttgcgacat gttaatact 
      ttggacgat gtagttcga ttggttaaa ggagcacaa 
      aatataata gttttaacc ggcgctgga gtctctgtt 
      tcgtgtgga attccagac tttcgatca cgtgatggg 
      atttacgtt cgactggcg atagatttt ccagatctt 
      ccagacccg caagcaatg tttgatatc agctatttc 
      agtcaagac cctcggccc ttcttcaaa ttcgcacga 
      gatatatac ccaggaaaa ttcacgcca agtccttgt 
      cacagattt ataaaaatg ttggaaaac tacggcaaa 
      ctcttgcgt aactacacc caaaacatt gacacactc 
      gaaaaagtt gcaaacata gaaaaagtg atcgaatgt 
      cacggttca ttcgcaacc gccacttgc acaaaatgt 
      ggacacaaa gtcaccgct gatgctatc agagagata 
      gttttagcg cagcaaatt ccactttgt gaaaaatgt 
      catccggga aaaacttcc gttccttgt atagaagaa 
      tataaagaa aacagtgag gaaatagat tatagacaa 
      cttgtgtct tcgggaatc atgaaacca gacattgtg 
      ttctttggc gagggactt ccagataca ttccacgaa 
      gcgatggct caagacaag acagagtgc gatttgctt 
      ttggttatt ggaagttcg ttgaaggtg cgacctgtt 
      gctctgatt cccagctcg ttgcctccg catgttcca 
      caaattctg atcaatcgg gagccttta ccgcattgc 
      cacttcgat gtggagctg ttgggagat tgcgacgtc 
      atcgtcaac cacttgtgc catttgttt ggcggtgtt 
      tggaaggaa ggcgttttc gacgacaac atgttggaa 
      gaagcctcg catttgctt ccattgcaa gagccgagt 
      ttcgacatg actcctcct tgtgttaaa agtcaaacc 
      gagccaaat tgtacagat gataactta gagaaagct 
      agcccgtgt ttatgcagt acaaaatgt agtagttcc 
      tcaacagta gaaaaagac gaagagtta acccttgaa 
      tgtagttgc aacaaacaa acgtcaatt attaataag 
      caatccttg ctggattcg gaccttgtc aattcatta 
      gatttaaaa tcggtcaaa gaacgccac atgtcagtg 
      gattctgcg cgggattcc ggcatcgga gacaattcg 
      aatttcacc gatttggag acgaaatac gacgacaca 
      tcggatgaa aacccggat ttgggcgaa tacaacaca 
      aacactaac actgctagt gatcctcaa tttacaaac 
      atatcgaat gatgaagcg aacaagacg aatacatat 
      ttggaaagt acaacaaac acttcagat ctgaaaggt 
      ttctggcaa ccgaaaatc aagaaaagc cttgcggaa 
      cgattgcca ccgaaatcg ttctatctt gtcaaaccg 
      agccgatat atttttcca ggcgctgag atttattac 
      gaccctgat gaaaagttt ggttattat gagggttca 
      tcgagttcg aacaactct gacagcgag tccgaaaac 
      ggtgaacca gtcagtaat tagttctag tttattact 
      taatttgtt ttgtttttg ttattaaat tcttaagca 
      tttt
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