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Gene information
General information
TC002557 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid23: 985297..1004324
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002557
    iBeetle screen iB_03406
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002557 RA
      MKFTEHLCA HITPEWRKQ YINYEEMKA MLYAAVEQA 
      PSAELVEPE ILTRYFAKF DEQFFSYCD KELTKINTF 
      YSEKLAEAT RKYAGLKSD LTEAQDVEY PRKKNSIKN 
      NILRKKNVP AKKIQELKL AFSEFYLSL ILLQNYQNL 
      NFTGFRKIL KKHDKLLNV DVGAKWRAE HVENSHFHT 
      NKDIDRLIR ETECTVTQE LEGGDRQRA MKRLRVPPL 
      GEQQIPWTT FKVGLFSGS FVVLMIAVI ISAIFHKRP 
      DDWRIVCRL YRGPFLIIE FLFLWGINI YGWRSSGVN 
      HVLIFEMDP RNHLSEQHI IEMAAIFGV IWCVSALSF 
      LYSTELSIP AYINPFVLV LLMSAFLFN PTKTLRHEA 
      RFWALRVLG KVLTAPFFY VTFADFWIA DQLNSIVNL 
      FTDIHYFFC FYLTNPSWS VGQDTNYCV EKHMIIRPF 
      MACLPAWFR FAQCLRRYR DTKEAFPHL ANAAKYATS 
      FFVVIFTAL NTAYSEGEA GMMESPFFY LWITASLIS 
      SCYAYTWDI KLDWGLFDS KAGDNKFLR EEIVYSSTW 
      FYYFAIVED FILRFGWAF SMSLTEMGY VHADLMVTI 
      LAPLEVFRR FVWNFFRLE NEHLNNCGK FRAVRDISV 
      APLDTSDQM LIVRMMDEE DGVVHRRKK KSGGKRKDE 
      GRSAEGEST DDMDI
    CDS sequence
    • >TC002557 RA
      atgaaattt actgaacat ctttgtgct catataact 
      ccagaatgg aggaaacaa tacataaat tacgaggag 
      atgaaggct atgttgtac gctgctgtg gaacaagcc 
      ccgtctgcg gaattggtt gagcctgaa attttgacc 
      cgatatttt gcaaaattc gacgaacag tttttctcc 
      tactgtgat aaggaactg acaaagatc aatacattt 
      tattctgaa aaattggcc gaagcgacc cgcaaatac 
      gctggtctg aaaagtgat ctcactgaa gctcaggat 
      gttgaatat ccacgcaag aaaaattct ataaaaaat 
      aatattcta agaaagaaa aacgtacct gcgaaaaaa 
      atacaagag ttgaagtta gcatttagc gaattttat 
      ttaagtcta atactgttg caaaattac cagaattta 
      aattttact gggtttcgg aaaattctg aaaaagcat 
      gataaattg ttaaatgta gacgtaggg gcgaaatgg 
      cgagcggaa catgtggaa aattcgcat tttcatacg 
      aataaagac atagatcga ttaattcga gagactgag 
      tgtacggtt actcaggag cttgaaggg ggcgatcgg 
      caaagggcc atgaaaagg ctcagggtt ccgccctta 
      ggggaacag caaattccg tggacgacg tttaaagtg 
      gggctgttt tcggggtcc tttgtggtg ctcatgatc 
      gcggtgatt atatcggcc atattccat aaacgtcca 
      gacgactgg cgtatcgtc tgtcgcctc taccgaggc 
      cctttcctc ataatcgaa tttttattt ctctggggt 
      attaacatt tacggctgg cggtcttct ggggttaat 
      cacgtattg atatttgaa atggacccc agaaatcat 
      ctttctgaa caacacatt atagaaatg gccgcaatt 
      ttcggtgtt atttggtgt gttagtgcc ctctctttt 
      ttgtacagt accgaatta agcattcca gcgtacatt 
      aacccattt gttcttgta cttttaatg tcagcattt 
      ctattcaat ccgaccaaa actctgagg catgaggcg 
      cgcttttgg gcccttcgc gttctgggt aaagtctta 
      acggctcca tttttctac gtaacattt gccgatttt 
      tggatcgcc gatcagtta aacagtatt gtaaacctt 
      ttcaccgac attcattac tttttttgt ttctatcta 
      accaacccc agttggtca gttggacaa gacacgaat 
      tattgtgtt gaaaagcac atgataata aggcctttt 
      atggcgtgt ttgccggct tggttcaga tttgcacaa 
      tgtttgaga agatataga gatactaag gaagcgttt 
      cctcatttg gctaatgca gcgaaatat gcgacgtca 
      ttctttgtt gtaatcttt acggcccta aacaccgct 
      tattcagaa ggcgaggcc ggaatgatg gagagcccc 
      tttttctat ctttggata acggcgtcg ctgataagt 
      tcttgctac gcatacact tgggatata aaattggac 
      tggggcctc ttcgactca aaagccgga gataacaag 
      ttcctcagg gaggaaatt gtttattcc tcgacgtgg 
      ttttattat ttcgcaata gtcgaggat tttatttta 
      cgattcgga tgggcattt tcgatgtct ttaaccgaa 
      atgggttat gttcacgcc gacctaatg gtaacgatt 
      ttagcgccc ttggaagtt tttaggaga ttcgtctgg 
      aactttttt cgccttgag aacgaacat ctcaataac 
      tgcggaaaa tttagggct gtgagagat atatcagtg 
      gcgcccttg gacacttcg gaccagatg ttgattgtg 
      aggatgatg gatgaggag gatggggtt gtgcacagg 
      aggaaaaag aagtccggg gggaaaagg aaggacgag 
      ggacgatcg gctgaagga gaatcaact gatgacatg 
      gacatttaa
    mRNA sequence
    • >TC002557 RA
      tcagtttca caataataa acaactaaa tgggttaat 
      cgaattcta ataatgaaa tttactgaa catctttgt 
      gctcatata actccagaa tggaggaaa caatacata 
      aattacgag gagatgaag gctatgttg tacgctgct 
      gtggaacaa gccccgtct gcggaattg gttgagcct 
      gaaattttg acccgatat tttgcaaaa ttcgacgaa 
      cagtttttc tcctactgt gataaggaa ctgacaaag 
      atcaataca ttttattct gaaaaattg gccgaagcg 
      acccgcaaa tacgctggt ctgaaaagt gatctcact 
      gaagctcag gatgttgaa tatccacgc aagaaaaat 
      tctataaaa aataatatt ctaagaaag aaaaacgta 
      cctgcgaaa aaaatacaa gagttgaag ttagcattt 
      agcgaattt tatttaagt ctaatactg ttgcaaaat 
      taccagaat ttaaatttt actgggttt cggaaaatt 
      ctgaaaaag catgataaa ttgttaaat gtagacgta 
      ggggcgaaa tggcgagcg gaacatgtg gaaaattcg 
      cattttcat acgaataaa gacatagat cgattaatt 
      cgagagact gagtgtacg gttactcag gagcttgaa 
      gggggcgat cggcaaagg gccatgaaa aggctcagg 
      gttccgccc ttaggggaa cagcaaatt ccgtggacg 
      acgtttaaa gtggggctg ttttcgggg tcctttgtg 
      gtgctcatg atcgcggtg attatatcg gccatattc 
      cataaacgt ccagacgac tggcgtatc gtctgtcgc 
      ctctaccga ggccctttc ctcataatc gaattttta 
      tttctctgg ggtattaac atttacggc tggcggtct 
      tctggggtt aatcacgta ttgatattt gaaatggac 
      cccagaaat catctttct gaacaacac attatagaa 
      atggccgca attttcggt gttatttgg tgtgttagt 
      gccctctct tttttgtac agtaccgaa ttaagcatt 
      ccagcgtac attaaccca tttgttctt gtactttta 
      atgtcagca tttctattc aatccgacc aaaactctg 
      aggcatgag gcgcgcttt tgggccctt cgcgttctg 
      ggtaaagtc ttaacggct ccatttttc tacgtaaca 
      tttgccgat ttttggatc gccgatcag ttaaacagt 
      attgtaaac cttttcacc gacattcat tactttttt 
      tgtttctat ctaaccaac cccagttgg tcagttgga 
      caagacacg aattattgt gttgaaaag cacatgata 
      ataaggcct tttatggcg tgtttgccg gcttggttc 
      agatttgca caatgtttg agaagatat agagatact 
      aaggaagcg tttcctcat ttggctaat gcagcgaaa 
      tatgcgacg tcattcttt gttgtaatc tttacggcc 
      ctaaacacc gcttattca gaaggcgag gccggaatg 
      atggagagc ccctttttc tatctttgg ataacggcg 
      tcgctgata agttcttgc tacgcatac acttgggat 
      ataaaattg gactggggc ctcttcgac tcaaaagcc 
      ggagataac aagttcctc agggaggaa attgtttat 
      tcctcgacg tggttttat tatttcgca atagtcgag 
      gattttatt ttacgattc ggatgggca ttttcgatg 
      tctttaacc gaaatgggt tatgttcac gccgaccta 
      atggtaacg attttagcg cccttggaa gtttttagg 
      agattcgtc tggaacttt tttcgcctt gagaacgaa 
      catctcaat aactgcgga aaatttagg gctgtgaga 
      gatatatca gtggcgccc ttggacact tcggaccag 
      atgttgatt gtgaggatg atggatgag gaggatggg 
      gttgtgcac aggaggaaa aagaagtcc ggggggaaa 
      aggaaggac gagggacga tcggctgaa ggagaatca 
      actgatgac atggacatt taattgcat ttaataaat 
      taaattatt tatttttaa atagagaaa tgtaaacgt 
      tttagagag taataaaga atatggttg tga