Status
Please wait ...
Gene information
General information
TC002552 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 23558040..23573980
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002552
    iBeetle screen iB_03403

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): more than 80%
    larval musculature mostly fluorescence not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 87.5% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    wings not closed - Number of animals showing the phenotype: 8

    metathoracic leg orientation irregular - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002552 RA
      MWLAVLLFF VFVVTYVIQ QVKKPPNFP PGPYWLPFV 
      GNLPQLKKL SKKLGGQHL ALSHLAKEY NTNVLGLKL 
      GKDYVVTVF TYPLIRDVL ISEEFEGRP DNFFLRLRC 
      MGQKRGITC TEGEEWNTL RNFVNRHLR NLGFGKKPM 
      EKMIQDEIC DILTILKKD GSDIQVDKF LAPCVLSVI 
      WTLITGEKI SRENNQLDE LLDLFNLRS KAFDMTGGT 
      LTQYPWLRY FLPEWSGFN LIKSVNTSL KNLFMKYIR 
      EHKDGWFEG RNEDLIYRY ISEMKTNTE ASKYFTDDH 
      LVMVCVDLF IGGAQTTSR TLGFAFLMM IMYPEVQKK 
      VQEQIDKHY DKNSSIEYS DRYKLPYVE AVLLETIRY 
      RYVVPIGGP RRVTKNTTL DGYYLPKNT TVLISFYSI 
      NNDPENWQN PEIFNPERF LDEKGSLLP DEKLIPFAL 
      GRRRCVGEI LAKNCIFLL FVEILRRYN VSLAPGSKP 
      PTGKPIAGI TLSPESYRV KFTERSL
    CDS sequence
    • >TC002552 RA
      atgtggctt gcagttttg ttgtttttc gtttttgtg 
      gtgacttat gtgatacaa caagtgaaa aaaccacct 
      aattttcct ccaggtcct tactggtta ccattcgtt 
      ggtaacctc ccccagctc aagaaactc tcaaaaaaa 
      ctaggcgga caacacctc gcgttatca catctagcc 
      aaagaatac aacaccaac gtcttaggc ctcaaatta 
      ggaaaggac tatgttgta acagttttt acatatccc 
      ctcatacgg gacgtccta attagcgaa gaattcgaa 
      ggaagaccc gacaacttc tttctacgt cttcggtgc 
      atgggccaa aaaagaggc atcacttgc accgaaggc 
      gaagaatgg aacacgtta cgaaacttt gtaaatcgc 
      cacttgcga aacttaggt ttcgggaaa aaaccaatg 
      gaaaaaatg atccaagac gagatttgc gacattcta 
      acaattctg aaaaaggac gggtccgac attcaagtc 
      gataaattt ttggctcct tgtgtccta agtgttatt 
      tggacgtta attacggga gaaaaaatt tcaagggaa 
      aataaccaa ttggacgaa ttgttggat ttgtttaat 
      ttgaggtcg aaagcgttt gatatgact ggaggtact 
      ctaacacag tatccctgg ttaaggtat tttttgccc 
      gaatggagt ggcttcaat ttgataaag tcggtaaac 
      acgagcttg aaaaatttg tttatgaaa tatattcgc 
      gagcataaa gatgggtgg tttgagggg agaaacgaa 
      gacttgata tatcgctac atttccgaa atgaagacc 
      aatacagaa gcctccaaa tatttcaca gatgatcat 
      ttggttatg gtctgtgtg gacctattt ataggcggt 
      gcccaaacc actagtcgg actttaggg tttgctttc 
      cttatgatg attatgtat ccagaagtg cagaaaaaa 
      gtgcaagag caaattgat aagcattat gataagaat 
      tcatccatt gagtattct gatcgttac aagttacct 
      tatgttgaa gcagtatta ttagaaaca ataagatat 
      cggtacgta gttccaatc gggggaccc cgaagagtc 
      acaaaaaac accacatta gatggctac tatttacca 
      aagaacacg actgtttta attagtttt tattcaatc 
      aacaacgac cccgaaaac tggcaaaac cccgaaatt 
      tttaacccg gaacgtttt ctagacgag aaaggttca 
      ttacttccc gacgaaaaa ctaatccct tttgcttta 
      gggcgtaga cgttgtgtt ggcgaaatc ttagcaaaa 
      aactgcatt tttttgttg tttgttgaa attttgcga 
      cggtataat gtgtcttta gcccccggt tcgaagcct 
      ccgactgga aaacctata gcaggaatt actttatct 
      ccagaaagc tacagagtt aagtttacg gaacggtcg 
      ttataa
    mRNA sequence
    • >TC002552 RA
      cagccgttt agttcgagt acgagtctg cagatgtct 
      accatgttt gcgtttgtc gaccgtttg cctggcttt 
      tggagataa aaccattcg agggacgag tgcaaagca 
      tacttacct ggcgtaggg gttaccgtg atcaagaag 
      gcggttccc ccagggtga ggcccttcc attgcactg 
      aggtcgggc tgacccctg cgattatcc ctaatgcgg 
      ataactcgg gcgcacaat ttttggcga acatttata 
      gtattgaag cgttttata ttgtacagc tgacacaaa 
      tattaacag ttttataca gctgagcta aaagtaccg 
      cctacaaat tggttcaag tgtggacta gacacacca 
      agtcgttgt aaccagcaa ccttgcttt agtaatagt 
      cgaggcttt accttacga tgttcgcca aatcacttt 
      cgtaacttg attcatctt tatcgacaa aggaattta 
      aaatgtggc ttgcagttt tgttgtttt tcgtttttg 
      tggtgactt atgtgatac aacaagtga aaaaaccac 
      ctaattttc ctccaggtc cttactggt taccattcg 
      ttggtaacc tcccccagc tcaagaaac tctcaaaaa 
      aactaggcg gacaacacc tcgcgttat cacatctag 
      ccaaagaat acaacacca acgtcttag gcctcaaat 
      taggaaagg actatgttg taacagttt ttacatatc 
      ccctcatac gggacgtcc taattagcg aagaattcg 
      aaggaagac ccgacaact tctttctac gtcttcggt 
      gcatgggcc aaaaaagag gcatcactt gcaccgaag 
      gcgaagaat ggaacacgt tacgaaact ttgtaaatc 
      gccacttgc gaaacttag gtttcggga aaaaaccaa 
      tggaaaaaa tgatccaag acgagattt gcgacattc 
      taacaattc tgaaaaagg acgggtccg acattcaag 
      tcgataaat ttttggctc cttgtgtcc taagtgtta 
      tttggacgt taattacgg gagaaaaaa tttcaaggg 
      aaaataacc aattggacg aattgttgg atttgttta 
      atttgaggt cgaaagcgt ttgatatga ctggaggta 
      ctctaacac agtatccct ggttaaggt attttttgc 
      ccgaatgga gtggcttca atttgataa agtcggtaa 
      acacgagct tgaaaaatt tgtttatga aatatattc 
      gcgagcata aagatgggt ggtttgagg ggagaaacg 
      aagacttga tatatcgct acatttccg aaatgaaga 
      ccaatacag aagcctcca aatatttca cagatgatc 
      atttggtta tggtctgtg tggacctat ttataggcg 
      gtgcccaaa ccactagtc ggactttag ggtttgctt 
      tccttatga tgattatgt atccagaag tgcagaaaa 
      aagtgcaag agcaaattg ataagcatt atgataaga 
      attcatcca ttgagtatt ctgatcgtt acaagttac 
      cttatgttg aagcagtat tattagaaa caataagat 
      atcggtacg tagttccaa tcgggggac cccgaagag 
      tcacaaaaa acaccacat tagatggct actatttac 
      caaagaaca cgactgttt taattagtt tttattcaa 
      tcaacaacg accccgaaa actggcaaa accccgaaa 
      tttttaacc cggaacgtt ttctagacg agaaaggtt 
      cattacttc ccgacgaaa aactaatcc cttttgctt 
      tagggcgta gacgttgtg ttggcgaaa tcttagcaa 
      aaaactgca tttttttgt tgtttgttg aaattttgc 
      gacggtata atgtgtctt tagcccccg gttcgaagc 
      ctccgactg gaaaaccta tagcaggaa ttactttat 
      ctccagaaa gctacagag ttaagttta cggaacggt 
      cgttataaa tatgtattg aacttagca aggttaaat 
      actcgaatg atagccaaa tgtaaaata gtgacgtca 
      gacacgtgt tctagtcct acatgttgt acattccga 
      atgcttgga gacggatcg agttatgta atagtggcc 
      tgtttaaag ttcactgtc aataaaaac tgattaatt 
      ag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY