Status
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Gene information
General information
TC002550 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 23595206..23649160
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002550
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03401

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands partially colour darker - Number of animals showing the phenotype: 7 (13 dpi)
    Days post injection



    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 7
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002550 RA
      MLLDMENHS TLLSLNMSP FNANSGDSP AQASSPMPQ 
      YGSPPVAYS SCQQSMGLP HHQPPMMMS QNMNNLDAS 
      YIFSSGASA ISGVDMGAS YQISGPATS LTAGDLPDT 
      KDGIEELCP VCGDKVSGY HYGLLTCES CKGFFKRTV 
      QNKKVYTCV AERSCHIDK TQRKRCPYC RFQKCLEVG 
      MKLEAVRAD RMRGGRNKF GPMYKRDRA RKLQMMRQR 
      QLAAQTLRG SSLGDAMYS SQPGTSPFA NIHIKQEIQ 
      IPQVSSLTS SPDSSPSPI AVALGQVNT SNLVQQASS 
      QQPALQIVG VPGGPSSMV MGPDNKLWG SANSTTTSP 
      HSLSPKAFQ FDTVVQGGS APPSSKVSP LIRDFVQAI 
      DDREWQNSL YTLLQNQTY NQCEVDLFE LMCKVLDQN 
      LFSQVDWAR NSVFFKDLK VDDQMKLLQ HSWSDMLVL 
      DHMHQRMHN NLPDEMTLH NGQKFDLLS LGLLGVPSL 
      ADHFTDITA KLQELKFDV SDYICVKFL LLLNPDVRG 
      ITNKKHVQE GYEQVQQAL LEYTVTCYP QIQDKFNKM 
      MQLLPEIHS LATRGEEHL YHKHCSGSA PTQTLLMEM 
      LHAKRK
    • >TC002550 RB
      MLLDMENHS TLLSLNMSP FNANSGDSP AQASSPMPQ 
      YGSPPVAYS SCQQSMGLP HHQPPMMMS QNMNNLDAS 
      YIFSSGASA ISGVDMGAS YQISGPATS LTAGDLPDT 
      KDGIEELCP VCGDKVSGY HYGLLTCES CKGFFKRTV 
      QNKKVYTCV AERSCHIDK TQRKRCPYC RFQKCLEVG 
      MKLEAVRAD RMRGGRNKF GPMYKRDRA RKLQMMRQR 
      QLAAQTLRG SSLGDAMYS SQPGTSPFA NIHIKQEIQ 
      IPQVSSLTS SPDSSPSPI AVALGQVNT SNLVQQASS 
      QQPALQIVG VPGGPSSMV MGPDNKLWG SANSTTTSP 
      HSLSPKAFQ FDTVVQGGS APPSSKVSP LIRDFVQAI 
      DDREWQNSL YTLLQNQTY NQCEVDLFE LMCKVLDQN 
      LFSQVDWAR NSVFFKDLK VDDQMKLLQ HSWSDMLVL 
      DHMHQRMHN NLPDEMTLH NGQKFDLLS LGLLGVPSL 
      ADHFTDITA KLQELKFDV SDYICVKFL LLLNPVYFL 
      DVRGITNKK HVQEGYEQV QQALLEYTV TCYPQIQDK 
      FNKMMQLLP EIHSLATRG EEHLYHKHC SGSAPTQTL 
      LMEMLHAKR K
    CDS sequence
    • >TC002550 RA
      atgttactg gacatggag aaccactcg acgttgtta 
      tctctgaac atgtccccg ttcaacgcc aactcgggg 
      gactcgccc gcccaggcc tccagcccc atgccccag 
      tacggctcg cccccggtg gcctacagc agctgccag 
      cagtccatg gggctgccg caccaccag ccccccatg 
      atgatgtcc cagaacatg aataaccta gacgcctct 
      tatatcttt tcttcaggg gccagcgcc atctccggc 
      gtcgacatg ggcgccagc taccagatt tccgggccg 
      gcgacgtcg ctcacagcc ggcgacctc ccagacacg 
      aaggacggc atcgaggag ctctgtccg gtctgcggg 
      gataaggtc tccgggtac cactacggc ctcctcacg 
      tgtgaatct tgcaaggga tttttcaaa cgaactgtt 
      caaaataag aaagtttac acgtgcgtg gcggaaaga 
      agttgtcac atagacaaa acgcaaagg aaaaggtgt 
      ccctactgc cggtttcaa aagtgctta gaagtcggg 
      atgaaactt gaagctgtc cgagcagac cgaatgaga 
      ggcggcagg aacaaattc ggtccgatg tacaaacgc 
      gaccgtgcc cgaaaactg caaatgatg cgacaacga 
      cagctggcg gcgcaaacg ctacgggga agctctctc 
      ggtgacgcc atgtacagc agccagccc ggaacatca 
      cccttcgcc aacatccac atcaagcaa gaaatccaa 
      attccgcaa gtgtcatct ctaacatcg tcgcccgac 
      tcgtcgccg agccccatc gccgtcgcc ctgggccag 
      gtcaacacc tccaacctg gtgcagcag gcgtccagc 
      cagcagccg gcgctgcag atcgtcggg gtgcccggg 
      gggccctcc tccatggtg atggggccc gacaacaag 
      ctctggggc tccgccaac tccaccacc acctcgccc 
      cactcgctc agccccaag gcgttccag ttcgacacg 
      gtggtgcaa gggggcagc gcgccgccc tccagtaaa 
      gtgtcgccg cttatcagg gactttgtg caagccatc 
      gacgatcgc gagtggcaa aactctttg tatacttta 
      ctccagaac caaacgtat aatcagtgt gaagtggac 
      ttatttgaa cttatgtgt aaagttttg gaccaaaat 
      ctgttctct caagtcgat tgggcgagg aattctgtg 
      ttctttaag gatctcaag gtggatgac caaatgaaa 
      ctactccaa cattcgtgg tcggatatg ctggttctg 
      gatcacatg caccaacgc atgcacaac aatcttcct 
      gacgaaatg acccttcac aacggtcag aagttcgat 
      ttgttaagt ttaggactc ttaggcgtg cccagttta 
      gcagaccac ttcactgac atcaccgcc aaactgcaa 
      gaactcaaa ttcgacgtt agcgactac atctgtgtt 
      aaatttctg ttgctgctt aaccccgat gtgcgagga 
      atcacaaac aagaaacat gtacaagaa ggttacgaa 
      caagttcaa caggcttta ctcgaatat acggtgacg 
      tgttaccca caaatccag gacaagttc aacaagatg 
      atgcaactg ctccctgag attcacagc ttggcaacg 
      cgcggcgaa gagcacctc taccacaag cactgcagc 
      ggtagcgcg ccaactcag acgctcctg atggaaatg 
      ctccacgcg aaaagaaaa taa
    • >TC002550 RB
      atgttactg gacatggag aaccactcg acgttgtta 
      tctctgaac atgtccccg ttcaacgcc aactcgggg 
      gactcgccc gcccaggcc tccagcccc atgccccag 
      tacggctcg cccccggtg gcctacagc agctgccag 
      cagtccatg gggctgccg caccaccag ccccccatg 
      atgatgtcc cagaacatg aataaccta gacgcctct 
      tatatcttt tcttcaggg gccagcgcc atctccggc 
      gtcgacatg ggcgccagc taccagatt tccgggccg 
      gcgacgtcg ctcacagcc ggcgacctc ccagacacg 
      aaggacggc atcgaggag ctctgtccg gtctgcggg 
      gataaggtc tccgggtac cactacggc ctcctcacg 
      tgtgaatct tgcaaggga tttttcaaa cgaactgtt 
      caaaataag aaagtttac acgtgcgtg gcggaaaga 
      agttgtcac atagacaaa acgcaaagg aaaaggtgt 
      ccctactgc cggtttcaa aagtgctta gaagtcggg 
      atgaaactt gaagctgtc cgagcagac cgaatgaga 
      ggcggcagg aacaaattc ggtccgatg tacaaacgc 
      gaccgtgcc cgaaaactg caaatgatg cgacaacga 
      cagctggcg gcgcaaacg ctacgggga agctctctc 
      ggtgacgcc atgtacagc agccagccc ggaacatca 
      cccttcgcc aacatccac atcaagcaa gaaatccaa 
      attccgcaa gtgtcatct ctaacatcg tcgcccgac 
      tcgtcgccg agccccatc gccgtcgcc ctgggccag 
      gtcaacacc tccaacctg gtgcagcag gcgtccagc 
      cagcagccg gcgctgcag atcgtcggg gtgcccggg 
      gggccctcc tccatggtg atggggccc gacaacaag 
      ctctggggc tccgccaac tccaccacc acctcgccc 
      cactcgctc agccccaag gcgttccag ttcgacacg 
      gtggtgcaa gggggcagc gcgccgccc tccagtaaa 
      gtgtcgccg cttatcagg gactttgtg caagccatc 
      gacgatcgc gagtggcaa aactctttg tatacttta 
      ctccagaac caaacgtat aatcagtgt gaagtggac 
      ttatttgaa cttatgtgt aaagttttg gaccaaaat 
      ctgttctct caagtcgat tgggcgagg aattctgtg 
      ttctttaag gatctcaag gtggatgac caaatgaaa 
      ctactccaa cattcgtgg tcggatatg ctggttctg 
      gatcacatg caccaacgc atgcacaac aatcttcct 
      gacgaaatg acccttcac aacggtcag aagttcgat 
      ttgttaagt ttaggactc ttaggcgtg cccagttta 
      gcagaccac ttcactgac atcaccgcc aaactgcaa 
      gaactcaaa ttcgacgtt agcgactac atctgtgtt 
      aaatttctg ttgctgctt aaccccgta tatttttta 
      gatgtgcga ggaatcaca aacaagaaa catgtacaa 
      gaaggttac gaacaagtt caacaggct ttactcgaa 
      tatacggtg acgtgttac ccacaaatc caggacaag 
      ttcaacaag atgatgcaa ctgctccct gagattcac 
      agcttggca acgcgcggc gaagagcac ctctaccac 
      aagcactgc agcggtagc gcgccaact cagacgctc 
      ctgatggaa atgctccac gcgaaaaga aaataa
    mRNA sequence
    • >TC002550 RA
      tgcgatgtt gtaagtgtg tgtgtgtgt gtatgtatg 
      acttcacaa cagcactaa catagacct ggttcatgg 
      gaaagtggg tcaccggtc ccgagtcac tttcactta 
      ttacatggg ctcctaatc attttccat tgtaacccc 
      gtgctgcca acacgtctc tcggaaggg gattcgcag 
      ttgaggcgc tcttggacg gtccgcgct ggtcagttt 
      gctcactga agtccgtcg agacggagg tgccgttgt 
      tccactggt gcgtgtgtg ttgtgtgcc tgctccacc 
      ggattactg tgctgtgca gtgtgcttg ctgatgctc 
      caccatgcc cttaaacta cttctcctt cgtggtgac 
      ctaaccgaa cgctcgtcc ctccgacca gtccttacc 
      ccgttgtac tgtttgtga aatgttact ggacatgga 
      gaaccactc gacgttgtt atctctgaa catgtcccc 
      gttcaacgc caactcggg ggactcgcc cgcccaggc 
      ctccagccc catgcccca gtacggctc gcccccggt 
      ggcctacag cagctgcca gcagtccat ggggctgcc 
      gcaccacca gccccccat gatgatgtc ccagaacat 
      gaataacct agacgcctc ttatatctt ttcttcagg 
      ggccagcgc catctccgg cgtcgacat gggcgccag 
      ctaccagat ttccgggcc ggcgacgtc gctcacagc 
      cggcgacct cccagacac gaaggacgg catcgagga 
      gctctgtcc ggtctgcgg ggataaggt ctccgggta 
      ccactacgg cctcctcac gtgtgaatc ttgcaaggg 
      atttttcaa acgaactgt tcaaaataa gaaagttta 
      cacgtgcgt ggcggaaag aagttgtca catagacaa 
      aacgcaaag gaaaaggtg tccctactg ccggtttca 
      aaagtgctt agaagtcgg gatgaaact tgaagctgt 
      ccgagcaga ccgaatgag aggcggcag gaacaaatt 
      cggtccgat gtacaaacg cgaccgtgc ccgaaaact 
      gcaaatgat gcgacaacg acagctggc ggcgcaaac 
      gctacgggg aagctctct cggtgacgc catgtacag 
      cagccagcc cggaacatc acccttcgc caacatcca 
      catcaagca agaaatcca aattccgca agtgtcatc 
      tctaacatc gtcgcccga ctcgtcgcc gagccccat 
      cgccgtcgc cctgggcca ggtcaacac ctccaacct 
      ggtgcagca ggcgtccag ccagcagcc ggcgctgca 
      gatcgtcgg ggtgcccgg ggggccctc ctccatggt 
      gatggggcc cgacaacaa gctctgggg ctccgccaa 
      ctccaccac cacctcgcc ccactcgct cagccccaa 
      ggcgttcca gttcgacac ggtggtgca agggggcag 
      cgcgccgcc ctccagtaa agtgtcgcc gcttatcag 
      ggactttgt gcaagccat cgacgatcg cgagtggca 
      aaactcttt gtatacttt actccagaa ccaaacgta 
      taatcagtg tgaagtgga cttatttga acttatgtg 
      taaagtttt ggaccaaaa tctgttctc tcaagtcga 
      ttgggcgag gaattctgt gttctttaa ggatctcaa 
      ggtggatga ccaaatgaa actactcca acattcgtg 
      gtcggatat gctggttct ggatcacat gcaccaacg 
      catgcacaa caatcttcc tgacgaaat gacccttca 
      caacggtca gaagttcga tttgttaag tttaggact 
      cttaggcgt gcccagttt agcagacca cttcactga 
      catcaccgc caaactgca agaactcaa attcgacgt 
      tagcgacta catctgtgt taaatttct gttgctgct 
      taaccccga tgtgcgagg aatcacaaa caagaaaca 
      tgtacaaga aggttacga acaagttca acaggcttt 
      actcgaata tacggtgac gtgttaccc acaaatcca 
      ggacaagtt caacaagat gatgcaact gctccctga 
      gattcacag cttggcaac gcgcggcga agagcacct 
      ctaccacaa gcactgcag cggtagcgc gccaactca 
      gacgctcct gatggaaat gctccacgc gaaaagaaa 
      ataacggcc gtccgcctc ttcgttttc gttaccata 
      tctgtgtgc aataccagc ttacaacac taatgtaca 
      taggttact ggaaatctc gtagttgtt gaaattgac 
      gatgccttc atgaagtac aaagagcta gggcggcca 
      ggccgcacc gccaccaca tatttttat aaaatagag 
      aaaaagcgg ccacagcac cctgctcct ccgttgttt 
      aatttatga taaatgtta cgttgattt aatgagttt 
      tataagaac gacacacac tgacacaca cacaaaaca 
      cattccaga tgtgctctg gagggattc aagggcatc 
      ctagcgtag ggtagcgta gcttttaag cgcgagtga 
      caaacaaac gaacgaagc gaatgagtg attggttgg 
      ttattgcag atcaagtgc actctcttc aacgtttta 
      atcttagta cgtgtgggg gtcgatttg ccccttgtt 
      agcttaacc aaggtgatg gactaacag ctgtaattt 
      tatgtaatt atagagtta tacatacta tatatacac 
      agtttatta ctagttatt aagtttaat tattatatt 
      ataaatata tttgattat gttacataa ttatatgaa 
      tgttaatat tatgtgaga gcaaggtac tgtggtcta 
      acctgtgtt attgttatc attaaattg gtaaatact 
      actgattgt ttgtgttgt gcaggctac ctctaaagt 
      ctccttcgc gtggggccg ctgtgcgag ccccaatgc 
      gctggtcag cggagtcta tttattatc tgttagagt 
      atcttgccc tgctgtgac atgcaaaag gccctgagc 
      ttactattg attgatcag atatatatt tatatataa 
      aatacgtac acttattta agcagtgtg attgagaag 
      atgtatata taatttatg tggatcaaa gtgacttgt 
      ttgtagaaa taaatcaca gtgaattaa
    • >TC002550 RB
      tgcgatgtt gtaagtgtg tgtgtgtgt gtatgtatg 
      acttcacaa cagcactaa catagacct ggttcatgg 
      gaaagtggg tcaccggtc ccgagtcac tttcactta 
      ttacatggg ctcctaatc attttccat tgtaacccc 
      gtgctgcca acacgtctc tcggaaggg gattcgcag 
      ttgaggcgc tcttggacg gtccgcgct ggtcagttt 
      gctcactga agtccgtcg agacggagg tgccgttgt 
      tccactggt gcgtgtgtg ttgtgtgcc tgctccacc 
      ggattactg tgctgtgca gtgtgcttg ctgatgctc 
      caccatgcc cttaaacta cttctcctt cgtggtgac 
      ctaaccgaa cgctcgtcc ctccgacca gtccttacc 
      ccgttgtac tgtttgtga aatgttact ggacatgga 
      gaaccactc gacgttgtt atctctgaa catgtcccc 
      gttcaacgc caactcggg ggactcgcc cgcccaggc 
      ctccagccc catgcccca gtacggctc gcccccggt 
      ggcctacag cagctgcca gcagtccat ggggctgcc 
      gcaccacca gccccccat gatgatgtc ccagaacat 
      gaataacct agacgcctc ttatatctt ttcttcagg 
      ggccagcgc catctccgg cgtcgacat gggcgccag 
      ctaccagat ttccgggcc ggcgacgtc gctcacagc 
      cggcgacct cccagacac gaaggacgg catcgagga 
      gctctgtcc ggtctgcgg ggataaggt ctccgggta 
      ccactacgg cctcctcac gtgtgaatc ttgcaaggg 
      atttttcaa acgaactgt tcaaaataa gaaagttta 
      cacgtgcgt ggcggaaag aagttgtca catagacaa 
      aacgcaaag gaaaaggtg tccctactg ccggtttca 
      aaagtgctt agaagtcgg gatgaaact tgaagctgt 
      ccgagcaga ccgaatgag aggcggcag gaacaaatt 
      cggtccgat gtacaaacg cgaccgtgc ccgaaaact 
      gcaaatgat gcgacaacg acagctggc ggcgcaaac 
      gctacgggg aagctctct cggtgacgc catgtacag 
      cagccagcc cggaacatc acccttcgc caacatcca 
      catcaagca agaaatcca aattccgca agtgtcatc 
      tctaacatc gtcgcccga ctcgtcgcc gagccccat 
      cgccgtcgc cctgggcca ggtcaacac ctccaacct 
      ggtgcagca ggcgtccag ccagcagcc ggcgctgca 
      gatcgtcgg ggtgcccgg ggggccctc ctccatggt 
      gatggggcc cgacaacaa gctctgggg ctccgccaa 
      ctccaccac cacctcgcc ccactcgct cagccccaa 
      ggcgttcca gttcgacac ggtggtgca agggggcag 
      cgcgccgcc ctccagtaa agtgtcgcc gcttatcag 
      ggactttgt gcaagccat cgacgatcg cgagtggca 
      aaactcttt gtatacttt actccagaa ccaaacgta 
      taatcagtg tgaagtgga cttatttga acttatgtg 
      taaagtttt ggaccaaaa tctgttctc tcaagtcga 
      ttgggcgag gaattctgt gttctttaa ggatctcaa 
      ggtggatga ccaaatgaa actactcca acattcgtg 
      gtcggatat gctggttct ggatcacat gcaccaacg 
      catgcacaa caatcttcc tgacgaaat gacccttca 
      caacggtca gaagttcga tttgttaag tttaggact 
      cttaggcgt gcccagttt agcagacca cttcactga 
      catcaccgc caaactgca agaactcaa attcgacgt 
      tagcgacta catctgtgt taaatttct gttgctgct 
      taaccccgt atatttttt agatgtgcg aggaatcac 
      aaacaagaa acatgtaca agaaggtta cgaacaagt 
      tcaacaggc tttactcga atatacggt gacgtgtta 
      cccacaaat ccaggacaa gttcaacaa gatgatgca 
      actgctccc tgagattca cagcttggc aacgcgcgg 
      cgaagagca cctctacca caagcactg cagcggtag 
      cgcgccaac tcagacgct cctgatgga aatgctcca 
      cgcgaaaag aaaataacg gccgtccgc ctcttcgtt 
      ttcgttacc atatctgtg tgcaatacc agcttacaa 
      cactaatgt acataggtt actggaaat ctcgtagtt 
      gttgaaatt gacgatgcc ttcatgaag tacaaagag 
      ctagggcgg ccaggccgc accgccacc acatatttt 
      tataaaata gagaaaaag cggccacag caccctgct 
      cctccgttg tttaattta tgataaatg ttacgttga 
      tttaatgag ttttataag aacgacaca cactgacac 
      acacacaaa acacattcc agatgtgct ctggaggga 
      ttcaagggc atcctagcg tagggtagc gtagctttt 
      aagcgcgag tgacaaaca aacgaacga agcgaatga 
      gtgattggt tggttattg cagatcaag tgcactctc 
      ttcaacgtt ttaatctta gtacgtgtg ggggtcgat 
      ttgcccctt gttagctta accaaggtg atggactaa 
      cagctgtaa ttttatgta attatagag ttatacata 
      ctatatata cacagttta ttactagtt attaagttt 
      aattattat attataaat atatttgat tatgttaca 
      taattatat gaatgttaa tattatgtg agagcaagg 
      tactgtggt ctaacctgt gttattgtt atcattaaa 
      ttggtaaat actactgat tgtttgtgt tgtgcaggc 
      tacctctaa agtctcctt cgcgtgggg ccgctgtgc 
      gagccccaa tgcgctggt cagcggagt ctatttatt 
      atctgttag agtatcttg ccctgctgt gacatgcaa 
      aaggccctg agcttacta ttgattgat cagatatat 
      atttatata taaaatacg tacacttat ttaagcagt 
      gtgattgag aagatgtat atataattt atgtggatc 
      aaagtgact tgtttgtag aaataaatc acagtgaat 
      taa
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